Team:NRP-UEA-Norwich/Safety RA

From 2014.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 17: Line 17:
     <link href="//maxcdn.bootstrapcdn.com/font-awesome/4.1.0/css/font-awesome.min.css" rel="stylesheet">
     <link href="//maxcdn.bootstrapcdn.com/font-awesome/4.1.0/css/font-awesome.min.css" rel="stylesheet">
     <style type="text/css">
     <style type="text/css">
-
       body{font-family:'Open Sans',sans-serif;font-size:13px}header{box-shadow:0 6px 6px rgba(0,0,0,.3)}header .container{height:80px}header .logo{padding:10px 0 10px 80px}header .igem{position:absolute;top:20px;right:-50px;z-index:100}header .igem img{width:40px;height:40px}header ul{float:right;list-style:none}header ul li{display:inline-block;white-space:nowrap}header ul li a{display:block;padding:0 15px;height:80px;line-height:80px;color:#000;text-decoration:none!important;transition:background-color .2s linear}header ul li a:active,header ul li a:hover,header ul li a:visited{background-color:#ecffeb;text-decoration:none!important;color:#000}header ul.dropdown-menu li{display:block}header ul.dropdown-menu li a{height:auto;padding:6px 20px;text-decoration:none}section{padding:50px 0}section h1{font-weight:300;font-size:48px;padding:0 0 50px}.dropdown-menu{margin:0}ul.menu li.dropdown:hover>ul.dropdown-menu{display:block}.bx-wrapper img{display:block;max-width:100%;min-width:970px;margin:0 auto}.welcome{padding:80px 0;background:url(https://static.igem.org/mediawiki/2014/0/06/Uea_header-bg.png)}.about{background:url(https://static.igem.org/mediawiki/2014/d/d7/Uea_body-bg.png)}.about h2{font-size:18px;font-weight:700}.about p.project{font-size:14px;padding:10px}.about p.project:last-of-type{padding-bottom:40px}.about .row:first-of-type{margin-bottom:40px}.about .col-md-4 i{transition:color .2s linear}.about .col-md-4:hover i{color:#2ECC71}.team{background:url(https://static.igem.org/mediawiki/2014/d/d7/Uea_body-bg.png);transition:all .3s linear;padding:0 0 40px}.team .img-responsive{border-radius:20em;-webkit-border-radius:20em;-moz-border-radius:20em;-webkit-filter:grayscale(100%);-moz-filter:grayscale(100%);-ms-filter:grayscale(100%);filter:grayscale(100%);filter:gray;transition:all .3s linear;max-width:200px;margin:0 auto}.team .col-md-4:hover .img-responsive{-webkit-filter:none;-moz-filter:none;-ms-filter:none;filter:none}.team .row:first-of-type{margin-bottom:40px}.team h2{font-size:18px;font-weight:700;color:#019875}.team h3{font-size:13px;font-weight:700;color:#019875}.team .col-md-3{padding:0 30px}.team .meetourteam{margin-bottom:60px}.social{background:url(https://static.igem.org/mediawiki/2014/d/d7/Uea_body-bg.png)}.sponsors .col-md-2{display:table;height:90px}.sponsors .middle{display:table-cell;vertical-align:middle}.sponsors .row:first-of-type{margin-bottom:40px}.general{padding-top:0;background:url(https://static.igem.org/mediawiki/2014/d/d7/Uea_body-bg.png)}.title{background:url(https://static.igem.org/mediawiki/2014/0/06/Uea_header-bg.png)}.title h1,.title h2{text-align:center}.title h1{margin-top:0;padding:40px 0;margin-bottom:40px}.title h2{font-size:18px;color:#999;margin:0 0 50px;padding-bottom:40px}.general p{margin:0 0 40px}.fa-8x{font-size:8em;color:#019875}.row .boxed-headline{width:100%;height:200px;background-color:#90C695}.container .overview{padding-bottom:40px}footer{color:#444;background-color:#FFFFF;padding:50px 0}footer ul.sponsors{float:left;width:100%;padding:0;margin:20px 0 0;list-style-type:none}footer ul.sponsors li{display:inline;padding:0 20px}footer ul.sponsors li img{width:90px}#top-section{border:0;height:14px;z-index:100;top:0;position:fixed;width:975px;left:50%;margin-left:-487px;background-color:transparent}#content,#globalWrapper{width:100%;height:100%;border:0;background-color:transparent;margin:0;padding:0;font-size:100%}#catlinks,#contentSub,#footer-box,#p-logo,#search-controls,#sitesub,.firstHeading,.printfooter,.visualClear{display:none}h1,h2,h3,h4,h5,h6{border-bottom:none}#bodyContent h1,#bodyContent h2{margin-bottom:40px}h3{color:#00B16A}
+
       body{font-family:'Open Sans',sans-serif;font-size:13px}header{box-shadow:0 6px 6px rgba(0,0,0,.3)}header .container{height:80px}header .logo{padding:10px 0 10px 80px}header .igem{position:absolute;top:20px;right:-50px;z-index:100}header .igem img{width:40px;height:40px}header ul{float:right;list-style:none}header ul li{display:inline-block;white-space:nowrap}header ul li a{display:block;padding:0 15px;height:80px;line-height:80px;color:#000;text-decoration:none!important;transition:background-color .2s linear}header ul li a:active,header ul li a:hover,header ul li a:visited{background-color:#ecffeb;text-decoration:none!important;color:#000}header ul.dropdown-menu li{display:block}header ul.dropdown-menu li a{height:auto;padding:6px 20px;text-decoration:none}section{padding:50px 0}section h1{font-weight:300;font-size:48px;padding:0 0 50px}.dropdown-menu{margin:0}ul.menu li.dropdown:hover>ul.dropdown-menu{display:block}.bx-wrapper img{display:block;max-width:100%;min-width:970px;margin:0 auto}.welcome{padding:80px 0;background:url(https://static.igem.org/mediawiki/2014/0/06/Uea_header-bg.png)}.about{background:url(https://static.igem.org/mediawiki/2014/d/d7/Uea_body-bg.png)}.about h2{font-size:18px;font-weight:700}.about p.project{font-size:14px;padding:10px}.about p.project:last-of-type{padding-bottom:40px}.about .row:first-of-type{margin-bottom:40px}.about .col-md-4 i{transition:color .2s linear}.about .col-md-4:hover i{color:#2ECC71}.team{background:url(https://static.igem.org/mediawiki/2014/d/d7/Uea_body-bg.png);transition:all .3s linear;padding:0 0 40px}.team .img-responsive{border-radius:20em;-webkit-border-radius:20em;-moz-border-radius:20em;-webkit-filter:grayscale(100%);-moz-filter:grayscale(100%);-ms-filter:grayscale(100%);filter:grayscale(100%);filter:gray;transition:all .3s linear;max-width:200px;margin:0 auto}.team .col-md-4:hover .img-responsive{-webkit-filter:none;-moz-filter:none;-ms-filter:none;filter:none}.team .row:first-of-type{margin-bottom:40px}.team h2{font-size:18px;font-weight:700;color:#019875}.team h3{font-size:13px;font-weight:700;color:#019875}.team .col-md-3{padding:0 30px}.team .meetourteam{margin-bottom:60px}.social{background:url(https://static.igem.org/mediawiki/2014/d/d7/Uea_body-bg.png)}.sponsors .col-md-2{display:table;height:90px}.sponsors .middle{display:table-cell;vertical-align:middle}.sponsors .row:first-of-type{margin-bottom:40px}.general{padding-top:0;background:url(https://static.igem.org/mediawiki/2014/d/d7/Uea_body-bg.png)}.title{background:url(https://static.igem.org/mediawiki/2014/0/06/Uea_header-bg.png)}.title h1,.title h2{text-align:center}.title h1{margin-top:0;padding:40px 0;margin-bottom:40px}.title h2{font-size:18px;color:#999;margin:0 0 50px;padding-bottom:40px}.general p{margin:0 0 40px}.fa-8x{font-size:8em;color:#019875}.row .boxed-headline{width:100%;height:200px;background-color:#90C695}.container .overview{padding-bottom:40px}footer{color:#444;background-color:#FFFFF;padding:50px 0}footer ul.sponsors{float:left;width:100%;padding:0;margin:20px 0 0;list-style-type:none}footer ul.sponsors li{display:inline;padding:0 20px}footer ul.sponsors li img{width:90px}#top-section{border:0;height:14px;z-index:100;top:0;position:fixed;width:975px;left:50%;margin-left:-487px;background-color:transparent}#content,#globalWrapper{width:100%;height:100%;border:0;background-color:transparent;margin:0;padding:0;font-size:100%}#catlinks,#contentSub,#footer-box,#p-logo,#search-controls,#sitesub,.firstHeading,.printfooter,.visualClear{display:none}h1,h2,h3,h4,h5,h6{border-bottom:none}#bodyContent h1,#bodyContent h2{margin-bottom:40px}h3{color:#019875;font-size:18px;font-weight:700}.col-md-1,.col-md-10,.col-md-11,.col-md-12,.col-md-2,.col-md-3,.col-md-4,.col-md-5,.col-md-6,.col-md-7,.col-md-8,.col-md-9{float:left}.col-md-12{width:100%}.col-md-11{width:91.66666667%}.col-md-10{width:83.33333333%}.col-md-9{width:75%}.col-md-8{width:66.66666667%}.col-md-7{width:58.33333333%}.col-md-6{width:50%}.col-md-5{width:41.66666667%}.col-md-4{width:33.33333333%}.col-md-3{width:25%}.col-md-2{width:16.66666667%}.col-md-1{width:8.33333333%}.container{min-width:970px}ul.bxslider{margin:0 !important}
     </style>
     </style>
   </head>
   </head>
Line 75: Line 75:
                   <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_CUT">The CUT event</a></li>
                   <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_CUT">The CUT event</a></li>
                   <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_School-Events">The Hewett School</a></li>
                   <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_School-Events">The Hewett School</a></li>
-
                   <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_Science-Cafe">Science Cafe</a></li>
+
                   <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_Science-Cafe">Science Café</a></li>
                   <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_Ethics">Ethics of Public Consultation</a></li>
                   <li><a href="https://2014.igem.org/Team:NRP-UEA-Norwich/HP_Ethics">Ethics of Public Consultation</a></li>
                 </ul>
                 </ul>
Line 104: Line 104:
        
        
       </div>
       </div>
-
      <div class="container">
 
-
     
 
-
      </div>
 
-
    </section>
 
  <div class="container">  
  <div class="container">  
-
<h3><b>COSHH Forms</b></h3>
+
<center>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2014/a/ad/Teaminthelab.jpg" width=690; height=500/>
 +
<p>
 +
</p>
 +
<h3><font color="019875"><b>COSHH Forms</b></font></h3>
-
We have developed 4 COSHH (Control of Substances Hazardous to Health) forms to cover <a href="https://static.igem.org/mediawiki/parts/1/11/NRP-UEA_COSHH_1.pdf" target="_blank">Bacterial Procedures</a>, <a href="https://static.igem.org/mediawiki/parts/e/eb/NRP-UEA_COSHH_2.pdf" target="_blank">SDS-Polyacrylamide Gel Electrophoresis</a> prcedures, <a href="https://static.igem.org/mediawiki/parts/1/16/NRP-UEA_COSHH_3.pdf" target="_blank">DNA Purification</a> from bacteria and analysis of DNA, and <a href="https://static.igem.org/mediawiki/parts/b/b3/NRP-UEA_COSHH_4.pdf" target="_blank">Agarose Gels and Ethidium Bromide Staining</a>. All student team members were required to read, sign and date each COSHH form before any laboratory work was allowed to begin.
+
We have developed 4 COSHH (Control of Substances Hazardous to Health) forms to cover <a href="https://static.igem.org/mediawiki/parts/1/11/NRP-UEA_COSHH_1.pdf" target="_blank">Bacterial Procedures</a>, <a href="https://static.igem.org/mediawiki/parts/e/eb/NRP-UEA_COSHH_2.pdf" target="_blank">SDS-Polyacrylamide Gel Electrophoresis</a> procedures, <a href="https://static.igem.org/mediawiki/parts/1/16/NRP-UEA_COSHH_3.pdf" target="_blank">DNA Purification from bacteria and analysis of DNA</a>, and <a href="https://static.igem.org/mediawiki/parts/b/b3/NRP-UEA_COSHH_4.pdf" target="_blank">Agarose Gels and Ethidium Bromide Staining</a>. All student team members were required to read, sign and date each COSHH form before any laboratory work was allowed to begin.
<p>
<p>
</p>
</p>
-
<h3><b>Risk Assessment</b></h3>
+
<h3><font color="019875"><b>Risk Assessment</b></font></h3>
-
A Genetic Modification risk assessment was submitted to the University of East Anglia to cover plant-based genetic modification brought about by <i>Agrobacterium tumefaciens</i> mediated expression in <i>Nicotiana benthamiana</i>. It is obvious from viewing this <a href="https://static.igem.org/mediawiki/parts/9/9e/NRP-UEA_GM_Risk_Assessment.pdf" target="_blank">Risk Assessment</a> that it has not yet been fully reviewed by the Safety Committee. The form was submitted to the Safety Committee at the beginning of August 2014. As this is the case, no meaningful plant experiments were carried out at UEA. Plant experiments were carried out at TSL instead under their safety regulations.
+
A Genetic Modification risk assessment was submitted to the University of East Anglia to cover plant-based genetic modification brought about by <i>Agrobacterium tumefaciens</i> mediated expression in <i>Nicotiana benthamiana</i>. It is obvious from viewing this <a href="https://static.igem.org/mediawiki/parts/9/9e/NRP-UEA_GM_Risk_Assessment.pdf" target="_blank">Risk Assessment</a> that it has not yet been fully reviewed by the Safety Committee. The form was submitted to the Safety Committee at the beginning of August 2014. Since permission to conduct genetic modification of plants at UEA was not provided by the end of our summer project, no meaningful plant experiments were carried out at UEA. Instead, plant experiments were carried out at TSL following the safety regulations of the host laboratory and institute.
<p>
<p>

Latest revision as of 20:39, 17 October 2014

NRP UEA Norwich iGEM 2014

Risk Assessments

COSHH Forms

We have developed 4 COSHH (Control of Substances Hazardous to Health) forms to cover Bacterial Procedures, SDS-Polyacrylamide Gel Electrophoresis procedures, DNA Purification from bacteria and analysis of DNA, and Agarose Gels and Ethidium Bromide Staining. All student team members were required to read, sign and date each COSHH form before any laboratory work was allowed to begin.

Risk Assessment

A Genetic Modification risk assessment was submitted to the University of East Anglia to cover plant-based genetic modification brought about by Agrobacterium tumefaciens mediated expression in Nicotiana benthamiana. It is obvious from viewing this Risk Assessment that it has not yet been fully reviewed by the Safety Committee. The form was submitted to the Safety Committee at the beginning of August 2014. Since permission to conduct genetic modification of plants at UEA was not provided by the end of our summer project, no meaningful plant experiments were carried out at UEA. Instead, plant experiments were carried out at TSL following the safety regulations of the host laboratory and institute.

A big thank you to our sponsors