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Team:TU Darmstadt/Notebook/Methods
From 2014.igem.org
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| - | <ul class="menu"><li class="first"><a href=" | + | <ul class="menu"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt" >Home</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Project" >Project</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results" >Results</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/PolicyandPractices" >Policy & Practices</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Achievemts" >Achievements</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a><ul class="menu2"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal" >Labjournal</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials</a></li><li class="active last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods" >Methods</a><ul class="menu3"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Agarose gel electrophoresis" >Agarose gel electrophoresis</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Dephosphorylation" >Dephosphorylation</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/DNA_ligation" >DNA ligation</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/DNA_quantification_with_NanoDrop" >DNA quantification with NanoDrop</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/PCR_ purification" >PCR purification</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/PCR_with_Pfu_polymerase" >PCR with Pfu polymerase</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Plasmid_preparation" >Plasmid preparation</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Restriction_digest" >Restriction digest</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/SDS-PAGE" >SDS-PAGE</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Bacterial_cell_culture" >Bacterial cell culture</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Cell_counting_plating" >Cell counting/plating</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Chemically_competent cells" >Chemically competent cells</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Colony_PCR_with_Taq_polymerase" >Colony PCR with Taq polymerase</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Glycerine_stocks" >Glycerine stocks</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Heat_shock_transformation" >Heat shock transformation</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Protein_expression" >Protein expression</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/37_hydrochloric_acid" >37% hydrochloric acid extraction</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Dichloromethane_extraction" >Dichloromethane extraction</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Methanol_extraction" >Methanol extraction</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Ethanol_extraction" >Ethanol extraction</a></li></ul></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Team" >Team</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Gallery" >Gallery</a></li></ul> |
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<!--TYPO3SEARCH_begin--><div id="c82" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Methods</h1></div><header><p>This page outlines our standardized protocols we used in the iGEM project 2014. If we changed one of the protocols for a specific reason, we mentioned it in the laboratory notebook. | <!--TYPO3SEARCH_begin--><div id="c82" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Methods</h1></div><header><p>This page outlines our standardized protocols we used in the iGEM project 2014. If we changed one of the protocols for a specific reason, we mentioned it in the laboratory notebook. | ||
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<p>For DNA | <p>For DNA | ||
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Agarose_gel_electrophoresis" title="Opens internal link in current window" class="internal-link">Agarose gel electrophoresis</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Dephosphorylation" title="Opens internal link in current window" class="internal-link">Dephosphorylation</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/DNA_ligation" title="Opens internal link in current window" class="internal-link">DNA ligation</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/DNA_quantification_with_NanoDrop" title="Opens internal link in current window" class="internal-link">DNA quantification with NanoDrop</a> |
</p> | </p> | ||
<p>- <a href="http://www.eurofinsgenomics.eu/media/892645/samplesubmissionguide_valuereadtube.pdf" title="Link to eurofinsgenomics.eu" target="_blank" class="external-link-new-window">DNA sequencing</a> | <p>- <a href="http://www.eurofinsgenomics.eu/media/892645/samplesubmissionguide_valuereadtube.pdf" title="Link to eurofinsgenomics.eu" target="_blank" class="external-link-new-window">DNA sequencing</a> | ||
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/PCR_purification" title="Opens internal link in current window" class="internal-link">PCR purification</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/PCR_with_Pfu_polymerase" title="Opens internal link in current window" class="internal-link">PCR with Pfu polymerase</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Plasmid_preparation" title="Opens internal link in current window" class="internal-link">Plasmid preparation</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Restriction_digest" title="Opens internal link in current window" class="internal-link">Restriction digest</a> |
</p> | </p> | ||
<p>For Proteins | <p>For Proteins | ||
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/SDS-PAGE" title="Opens internal link in current window" class="internal-link">SDS-PAGE</a> |
</p> | </p> | ||
| - | <p> | + | <p></p> |
<p><b>In vivo methods</b> | <p><b>In vivo methods</b> | ||
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Bacterial_cell_culture" title="Opens internal link in current window" class="internal-link">Bacterial cell culture</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Cell_counting_plating" title="Opens internal link in current window" class="internal-link">Cell counting/plating</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Chemically_competent_cells" title="Opens internal link in current window" class="internal-link">Chemically competent cells</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Colony_PCR_with_Taq_polymerase" title="Opens internal link in current window" class="internal-link">Colony PCR with Taq polymerase</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Glycerine_stocks" title="Opens internal link in current window" class="internal-link">Glycerine stocks</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Heat_shock_transformation" title="Opens internal link in current window" class="internal-link">Heat shock transformation</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Protein_expression" title="Opens internal link in current window" class="internal-link">Protein expression</a></p></header><ul style="line-height: 1.5em; list-style: none; margin: 0.3em 50px 0px; padding: 0px; text-align: justify; "></ul><p style="margin: 0px 50px 15px; line-height: 1.5em; text-align: justify; color: white; font-family: 'Arial regular'; font-size: medium; "</p></div><div id="c245" class="csc-default"><p><b>Anthocyanin extraction methods</b> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/37_hydrochloric_acid" title="Opens internal link in current window" class="internal-link">37% hydrochloric acid</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Dichloromethane" title="Opens internal link in current window" class="internal-link">Dichloromethane</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Methanol" title="Opens internal link in current window" class="internal-link">Methanol</a> |
</p> | </p> | ||
| - | <p>- <a href=" | + | <p>- <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods/Ethanol" title="Opens internal link in current window" class="internal-link">Ethanol</a> |
</p> | </p> | ||
<p> </p> | <p> </p> | ||
Latest revision as of 00:28, 18 October 2014
Methods
This page outlines our standardized protocols we used in the iGEM project 2014. If we changed one of the protocols for a specific reason, we mentioned it in the laboratory notebook.
In vitro methods
For DNA
- DNA quantification with NanoDrop
For Proteins
- SDS-PAGE
In vivo methods
