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Team:Cornell/notebook/protocols
From 2014.igem.org
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<div class="row"> | <div class="row"> | ||
| - | + | <div class="col-md-9 col-xs-12"> | |
| - | <div class="col-md- | + | <div class="thumbnail"> |
| + | <img src="https://static.igem.org/mediawiki/2014/6/60/Cornell_IMG_4991_small.jpg"> | ||
| + | </div> | ||
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| + | <div class="col-md-3 col-xs-6" style="margin-top: 70px;"> | ||
<h3>DNA Purification</h3> | <h3>DNA Purification</h3> | ||
| - | <ul | + | <ul> |
<li> | <li> | ||
<a href="https://static.igem.org/mediawiki/2012/3/35/Ezna_Miniprep.pdf" target="_blank">EZNA Miniprep</a> | <a href="https://static.igem.org/mediawiki/2012/3/35/Ezna_Miniprep.pdf" target="_blank">EZNA Miniprep</a> | ||
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<a href="https://static.igem.org/mediawiki/2012/2/2d/Qiagen_Miniprep.pdf" target="_blank">Qiagen Miniprep</a> | <a href="https://static.igem.org/mediawiki/2012/2/2d/Qiagen_Miniprep.pdf" target="_blank">Qiagen Miniprep</a> | ||
</li> | </li> | ||
| - | <li> | + | <li> |
<a href="https://static.igem.org/mediawiki/2013/7/79/Column_Purification.pdf" target="_blank">Column Purification</a> | <a href="https://static.igem.org/mediawiki/2013/7/79/Column_Purification.pdf" target="_blank">Column Purification</a> | ||
</li> | </li> | ||
</ul> | </ul> | ||
| - | + | <h3>PCR Methods</h3> | |
| - | + | <ul> | |
| - | + | <li> | |
| - | + | <a href="https://static.igem.org/mediawiki/2013/3/30/Q5_PCR.pdf" target="_blank">Q5 PCR</a> | |
| - | + | ||
| - | <a href="https://static.igem.org/mediawiki/ | + | |
</li> | </li> | ||
<li> | <li> | ||
| - | <a href="https://static.igem.org/mediawiki/2012/ | + | <a href="https://static.igem.org/mediawiki/2012/2/26/Phusion_PCR.pdf" target="_blank">Phusion PCR</a> |
</li> | </li> | ||
<li> | <li> | ||
| - | <a href="https://static.igem.org/mediawiki/2012/ | + | <a href="https://static.igem.org/mediawiki/2012/4/4b/Taq_PCR.pdf" target="_blank">Taq PCR</a> |
</li> | </li> | ||
<li> | <li> | ||
| - | <a href="https://static.igem.org/mediawiki/2012/ | + | <a href="https://static.igem.org/mediawiki/2012/3/33/Vent_PCR.pdf" target="_blank">Vent PCR</a> |
</li> | </li> | ||
| - | + | </ul> | |
| + | </div> | ||
</div> | </div> | ||
| - | + | <div class="row"> | |
| - | + | <div class="col-md-3 col-xs-4"> | |
| - | + | <h3>Metal and Growth Quantification</h3> | |
| - | + | <ul> | |
| - | + | ||
| - | + | ||
<li> | <li> | ||
| - | <a href="https://static.igem.org/mediawiki/ | + | <a href="https://static.igem.org/mediawiki/2014/f/f6/Cornell_Growth_Experiment_Protocol.pdf" target="_blank">Growth Experiments</a> |
</li> | </li> | ||
<li> | <li> | ||
| - | <a href="https://static.igem.org/mediawiki/ | + | <a href="https://static.igem.org/mediawiki/2014/e/e3/Cornell_Batch_Metal_Sequestration_Experiments.pdf" target="_blank">Batch Metal Sequestration Experiments</a> |
</li> | </li> | ||
| - | + | <li> | |
| - | <a href="https://static.igem.org/mediawiki/ | + | <a href="https://static.igem.org/mediawiki/2014/1/14/Cornell_Phen_Green_SK_Metal_Sequestration_Experiments.pdf" target="_blank">Kinetic Metal Sequestration Experiments</a> |
</li> | </li> | ||
| - | </ul> | + | </ul> |
| - | </div | + | </div> |
| - | + | <div class="col-md-3 col-xs-4"> | |
| - | + | ||
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| - | <div class="col-md- | + | |
<h3>Plasmid Insertion</h3> | <h3>Plasmid Insertion</h3> | ||
| - | <ul | + | <ul> |
| - | + | <li> | |
<a href="https://static.igem.org/mediawiki/2012/0/07/Ecoli_electroporation.pdf" target="_blank"><i>E.coli</i> Electroporation</a> | <a href="https://static.igem.org/mediawiki/2012/0/07/Ecoli_electroporation.pdf" target="_blank"><i>E.coli</i> Electroporation</a> | ||
</li> | </li> | ||
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<a href="https://static.igem.org/mediawiki/2014/9/99/Cornell_iGEM_Chemically_Competent_Stocks.pdf" target="_blank">Preparing Chemically Compotent <i>E.coli</i></a> | <a href="https://static.igem.org/mediawiki/2014/9/99/Cornell_iGEM_Chemically_Competent_Stocks.pdf" target="_blank">Preparing Chemically Compotent <i>E.coli</i></a> | ||
</li> | </li> | ||
| - | + | </ul> | |
| - | </div> | + | </div> |
| - | + | <div class="col-md-3 col-xs-4"> | |
| - | + | <h3>DNA Assessment</h3> | |
| - | + | <ul> | |
| - | + | <li> | |
<a href="https://static.igem.org/mediawiki/2012/6/6c/DNA_Gel_Electrophoresis.pdf" target="_blank">DNA Gel Electrophoresis</a> | <a href="https://static.igem.org/mediawiki/2012/6/6c/DNA_Gel_Electrophoresis.pdf" target="_blank">DNA Gel Electrophoresis</a> | ||
</li> | </li> | ||
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<a href="https://static.igem.org/mediawiki/2012/0/03/Sequencing_Preparation.pdf" target="_blank">Preparation of DNA for Sequencing</a> | <a href="https://static.igem.org/mediawiki/2012/0/03/Sequencing_Preparation.pdf" target="_blank">Preparation of DNA for Sequencing</a> | ||
</li> | </li> | ||
| - | + | </ul> | |
| - | </div> | + | </div> |
| - | + | <div class="col-md-3 col-xs-4"> | |
| - | + | <h3>Plasmid Editing</h3> | |
| - | + | <ul> | |
| - | + | <li> | |
| - | <a href="https://static.igem.org/mediawiki/ | + | <a href="https://static.igem.org/mediawiki/2012/a/af/Double_Digest.pdf" target="_blank">Double Digest</a> |
</li> | </li> | ||
<li> | <li> | ||
| - | <a href="https://static.igem.org/mediawiki/ | + | <a href="https://static.igem.org/mediawiki/2012/f/fb/Single_Digest.pdf" target="_blank">Single Digest</a> |
</li> | </li> | ||
| - | + | <li> | |
| - | <a href="https://static.igem.org/mediawiki/ | + | <a href="https://static.igem.org/mediawiki/2012/a/a5/Site_Directed_Mutagenesis.pdf" target="_blank">Site Directed Mutagenesis</a> |
</li> | </li> | ||
| - | + | ||
| - | </ | + | <li> |
| - | </ | + | <a href="https://static.igem.org/mediawiki/2014/e/ef/Vector_Dephosphorylation.pdf" target="_blank">Vector Dephosphorylation</a> |
| - | </ | + | </li> |
| - | + | ||
| - | </div> | + | <li> |
| + | <a href="https://static.igem.org/mediawiki/2012/a/a7/T4_Ligase_Ligation.pdf" target="_blank">T4 Ligase Ligation</a> | ||
| + | </li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | </div> | ||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 02:51, 18 October 2014
Protocols
