Team:Hong Kong HKUST/riboregulator/parts

From 2014.igem.org

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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379015">BBa_K1379015</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379015">BBa_K1379015</a></td>  
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  <td>Lock 3C RBS</td>       
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  <td>RBS</td>       
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  <td>B0032 derivate for Lock 3C</td>    
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  <td>RBS(Lock 3c)</td>    
  <td>12</td>
  <td>12</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379018">BBa_K1379018</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379018">BBa_K1379018</a></td>  
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  <td>HKUST Lock 1</td>       
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  <td>RNA</td>       
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  <td>Cis-Repressor HKUST Lock 1</td>    
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  <td>HKUST Lock 1</td>    
  <td>45</td>
  <td>45</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379019">BBa_K1379019</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379019">BBa_K1379019</a></td>  
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  <td>HKUST Key 1</td>       
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  <td>RNA</td>       
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  <td>Trans-Activator for HKUST Key 1</td>    
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  <td>HKUST Key 1</td>    
  <td>82</td>
  <td>82</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379020">BBa_K1379020</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379020">BBa_K1379020</a></td>  
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  <td>Cis-repressed GFP Lock 1</td>       
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  <td>Intermediate</td>       
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  <td>Cis-repressed GFP Generator Lock 1</td>    
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  <td>Lock 1-GFP-B0015</td>    
  <td>903</td>
  <td>903</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379022">BBa_K1379022</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379022">BBa_K1379022</a></td>  
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  <td>Cis-repressed GFP Generator Lock 3</td>       
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  <td>Intermediate</td>       
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  <td>Cis-repressed GFP Generator Lock 3 </td>    
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  <td>Lock 3-GFP-B0015</td>    
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  <td>905</td>
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  <td>903</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379023">BBa_K1379023</a></td>
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  <td>Intermediate</td>     
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  <td>Key 3-B0015</td>  
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  <td>231</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379024">BBa_K1379024</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379024">BBa_K1379024</a></td>  
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  <td>Cis-repressed GFP Lock 3C</td>       
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  <td>Intermediate</td>       
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  <td>Cis-repressed GFP Generator Lock 3C</td>    
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  <td>Lock 3c-GFP-B0015</td>    
  <td>905</td>
  <td>905</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379026">BBa_K1379026</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379026">BBa_K1379026</a></td>  
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  <td>Cis-repressed GFP Lock MCR</td>       
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  <td>Intermediate</td>       
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  <td>Cis-repressed GFP Generator MCR</td>    
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  <td>K175031-GFP-B0015</td>    
  <td>910</td>
  <td>910</td>
</tr>  
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379027">BBa_K1379027</a></td>
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  <td>Intermediate</td>     
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  <td>K175032-B0015</td>  
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  <td>221</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379028">BBa_K1379028</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379028">BBa_K1379028</a></td>  
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  <td>Cis-repressed GFP HKUST Lock 1</td>       
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  <td>Intermediate</td>       
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  <td>Cis-repressed GFP Generator HKUST Lock 1</td>    
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  <td>HKUST Lock 1-GFP-B0015</td>    
  <td>908</td>
  <td>908</td>
</tr>  
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379029">BBa_K1379029</a></td>
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  <td>Intermediate</td>     
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  <td>HKUST Key 1-B0015</td>  
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  <td>219</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379030">BBa_K1379030</a></td>
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  <td>Measurement</td>     
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  <td>J23102-Lock 1-GFP-B0015</td>  
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  <td>946</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379032">BBa_K1379032</a></td>
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  <td>Measurement</td>     
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  <td>J23102-Lock 3-GFP-B0015</td>  
 +
  <td>948</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379033">BBa_K1379033</a></td>
 +
  <td>Intermediate</td>     
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  <td>pBad/araC-Key 3</td>  
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  <td>1312</td>
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</tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379034">BBa_K1379034</a></td>
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  <td>Measurement</td>     
 +
  <td>J23102-Lock 3c-GFP-B0015</td>  
 +
  <td>948</td>
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</tr>
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 +
                                <tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379035">BBa_K1379035</a></td>
 +
  <td>Intermediate</td>     
 +
  <td>pBad/araC-Key 3c</td>  
 +
  <td>1312</td>
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</tr>
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 +
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379036">BBa_K1379036</a></td>
 +
  <td>Measurement</td>     
 +
  <td>J23102-K175031-GFP-B0015</td>  
 +
  <td>953</td>
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</tr>
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 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379037">BBa_K1379037</a></td>
 +
  <td>Intermediate</td>     
 +
  <td>pBad/araC-K175032</td>  
 +
  <td>1302</td>
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</tr>
 +
 
 +
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379038">BBa_K1379038</a></td>
 +
  <td>Measurement</td>     
 +
  <td>J23102-HKUST Lock 1-GFP-B0015</td>  
 +
  <td>951</td>
 +
</tr>
 +
 
 +
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 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379039">BBa_K1379039</a></td>
 +
  <td>Intermediate</td>     
 +
  <td>pBad/araC-HKUST Key 1</td>  
 +
  <td>1300</td>
 +
</tr>                         
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<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379046">BBa_K1379046</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379046">BBa_K1379046</a></td>  
-
  <td>Lock 1 RBS GFP generator</td>       
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  <td>Intermediate</td>       
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  <td>GFP generator using a Lock 1 modified RBS</td>    
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  <td>RBS(Lock 1)-GFP-B0015</td>    
  <td>873</td>
  <td>873</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379047">BBa_K1379047</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379047">BBa_K1379047</a></td>  
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  <td>Lock 3C RBS GFP generator</td>       
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  <td>Intermediate</td>       
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  <td>GFP generator using a Lock 3C modified RBS</td>    
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  <td>RBS(Lock 3c)-GFP-B0015</td>    
  <td>875</td>
  <td>875</td>
</tr>  
</tr>  
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a></td>
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  <td>Inducible pBad/araC promoter</td>     
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  <td>Inducible pBad/araC promoter</td>  
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-
  <td>1210</td>
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<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01010">BBa_J01010</a></td>
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  <td>Riboregulator Lock 1</td>     
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-
  <td>Biobrick version of Isaacs' riboregulator cis repressed lock, crR12.</td>  
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-
  <td>40</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175031">BBa_K175031</a></td>
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  <td>Lock for medium RBS (B0032) from the lock/key library TUD09</td>     
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-
  <td>This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS.</td>  
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-
  <td>47</td>
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</tr>
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<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175032">BBa_K175032</a></td>
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  <td>Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09</td>     
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-
  <td>This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031).</td>  
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-
  <td>84</td>
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</tr>
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-
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-
<tr>
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-
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23080">BBa_J23080</a></td>
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-
  <td>B0034 derived RBS to match spacing of J01122</td>     
+
-
  <td>RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122.</td>  
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-
  <td>10</td>
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-
</tr>
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-
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<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379048">BBa_K1379048</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379048">BBa_K1379048</a></td>  
-
  <td>Cis repressed GFP, with Trans-activator</td>       
+
  <td>Measurement</td>       
-
  <td>Repression measurement device 1 with trans activator</td>    
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  <td>J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015</td>    
  <td>2403</td>
  <td>2403</td>
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Latest revision as of 22:06, 17 October 2014



Riboregulator Parts

Name Type Description Length
BBa_K1379015 RBS RBS(Lock 3c) 12
BBa_K1379018 RNA HKUST Lock 1 45
BBa_K1379019 RNA HKUST Key 1 82
BBa_K1379020 Intermediate Lock 1-GFP-B0015 903
BBa_K1379022 Intermediate Lock 3-GFP-B0015 903
BBa_K1379023 Intermediate Key 3-B0015 231
BBa_K1379024 Intermediate Lock 3c-GFP-B0015 905
BBa_K1379026 Intermediate K175031-GFP-B0015 910
BBa_K1379027 Intermediate K175032-B0015 221
BBa_K1379028 Intermediate HKUST Lock 1-GFP-B0015 908
BBa_K1379029 Intermediate HKUST Key 1-B0015 219
BBa_K1379030 Measurement J23102-Lock 1-GFP-B0015 946
BBa_K1379032 Measurement J23102-Lock 3-GFP-B0015 948
BBa_K1379033 Intermediate pBad/araC-Key 3 1312
BBa_K1379034 Measurement J23102-Lock 3c-GFP-B0015 948
BBa_K1379035 Intermediate pBad/araC-Key 3c 1312
BBa_K1379036 Measurement J23102-K175031-GFP-B0015 953
BBa_K1379037 Intermediate pBad/araC-K175032 1302
BBa_K1379038 Measurement J23102-HKUST Lock 1-GFP-B0015 951
BBa_K1379039 Intermediate pBad/araC-HKUST Key 1 1300
BBa_K1379046 Intermediate RBS(Lock 1)-GFP-B0015 873
BBa_K1379047 Intermediate RBS(Lock 3c)-GFP-B0015 875
BBa_K1379048 Measurement J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015 2403

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