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Team:Hannover/Primer
From 2014.igem.org
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| - | <h1>Primer</h1><p class="text"> Here you find a list | + | <h1><a href="https://2014.igem.org/Team:Hannover/Protocols">Protocols</a> / Primer</h1><p class="text"> Here you find a list including the oligonucleotides used.</p> |
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| - | <tr><td><b> | + | <tr><td><b>Name</b></td><td><b><p class="text" >Sequence in 5' → 3' Direction</p></b></td></tr> |
| - | <tr><td>01_pORE_E3_2x35S fwd</td><td>agcgtcggcgaggctaaaaagaacaaatgt</td></tr> | + | <tr><td>01_pORE_E3_2x35S fwd</td><td><p class="text" style="text-transform:lowercase;">agcgtcggcgaggctaaaaagaacaaatgt</p></td></tr> |
| - | <tr><td>02_pORE_E3_2x35S rev</td><td><p class="text" style="text-transform:lowercase;">ggaaGGATCCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAA | + | <tr><td>02_pORE_E3_2x35S rev</td><td><p class="text" style="text-transform:lowercase;">ggaaGGATCCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAA</p></td></tr> |
<tr><td>03_Oligo1_RCF[21]</td><td><p class="text" style="text-transform:lowercase;">GATCGAATTCAGATCTGGATCCtaaCTCGAG</p></td></tr> | <tr><td>03_Oligo1_RCF[21]</td><td><p class="text" style="text-transform:lowercase;">GATCGAATTCAGATCTGGATCCtaaCTCGAG</p></td></tr> | ||
<tr><td>04_Oligo2_RCF[21]</td><td><p class="text" style="text-transform:lowercase;">CGCGCTCGAGttaGGATCCAGATCTGAATTC</p></td></tr> | <tr><td>04_Oligo2_RCF[21]</td><td><p class="text" style="text-transform:lowercase;">CGCGCTCGAGttaGGATCCAGATCTGAATTC</p></td></tr> | ||
<tr><td>05_F1_gfp_EMP</td><td><p class="text" style="text-transform:lowercase;">AGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTC</p></td></tr> | <tr><td>05_F1_gfp_EMP</td><td><p class="text" style="text-transform:lowercase;">AGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTC</p></td></tr> | ||
<tr><td>06_R1_gfp_EMP</td><td><p class="text" style="text-transform:lowercase;">CCGGACTTGTCATCGTCGTCCTTGTAGTCTTTGTATAGTTCATCCATGCCATGTGTAATCCCAG</p></td></tr> | <tr><td>06_R1_gfp_EMP</td><td><p class="text" style="text-transform:lowercase;">CCGGACTTGTCATCGTCGTCCTTGTAGTCTTTGTATAGTTCATCCATGCCATGTGTAATCCCAG</p></td></tr> | ||
| - | <tr><td>07_R2_gfp_EMP</td><td>agcgtcggcgaggctaaaaagaacaaatgt</p></td></tr> | + | <tr><td>07_R2_gfp_EMP</td><td><p class="text" style="text-transform:lowercase;">agcgtcggcgaggctaaaaagaacaaatgt</p></td></tr> |
<tr><td>08_TMBP_pASK_fwd</td><td><p class="text" style="text-transform:lowercase;">ggaaGAATTCgGACGAtGATGAtAAGGTGGTTATCAACGGCGTCAAGT</p></td></tr> | <tr><td>08_TMBP_pASK_fwd</td><td><p class="text" style="text-transform:lowercase;">ggaaGAATTCgGACGAtGATGAtAAGGTGGTTATCAACGGCGTCAAGT</p></td></tr> | ||
<tr><td>09_TMBP_pASK_rev</td><td><p class="text" style="text-transform:lowercase;">ggaaCCATGGgtTTGTCgTCGTCaTCCTTACAGCTGCAAGATGATCCGC</p></td></tr> | <tr><td>09_TMBP_pASK_rev</td><td><p class="text" style="text-transform:lowercase;">ggaaCCATGGgtTTGTCgTCGTCaTCCTTACAGCTGCAAGATGATCCGC</p></td></tr> | ||
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<tr><td>14_CBD in pSB1C3 fwd</td><td><p class="text" style="text-transform:lowercase;">aaGAATTCGCGGCCGCTTCTAGATGACCAGTTCTATGTCAGTAGAATTTT</p></td></tr> | <tr><td>14_CBD in pSB1C3 fwd</td><td><p class="text" style="text-transform:lowercase;">aaGAATTCGCGGCCGCTTCTAGATGACCAGTTCTATGTCAGTAGAATTTT</p></td></tr> | ||
<tr><td>15_CBD in pSB1C3 rev</td><td><p class="text" style="text-transform:lowercase;">aaCTGCAGCGGCCGCTACTAGTAttattaaCGGCCTGACGCAAATCC</p></td></tr> | <tr><td>15_CBD in pSB1C3 rev</td><td><p class="text" style="text-transform:lowercase;">aaCTGCAGCGGCCGCTACTAGTAttattaaCGGCCTGACGCAAATCC</p></td></tr> | ||
| - | <tr><td>16_VF2</td><td>tgccacctgacgtctaagaa</td></tr> | + | <tr><td>16_VF2</td><td><p class="text" style="text-transform:lowercase;">tgccacctgacgtctaagaa</p></td></tr> |
| - | <tr><td>17_VR</td><td>attaccgcctttgagtgagc</td></tr> | + | <tr><td>17_VR</td><td><p class="text" style="text-transform:lowercase;">attaccgcctttgagtgagc</p></td></tr> |
<tr><td>T7</td><td><p class="text" style="text-transform:lowercase;">CGACTCACTATAGGGCG</p></td></tr> | <tr><td>T7</td><td><p class="text" style="text-transform:lowercase;">CGACTCACTATAGGGCG</p></td></tr> | ||
<tr><td>BackSeq-pGII</td><td><p class="text" style="text-transform:lowercase;">attaccggcctttgagtgagc</p></td></tr> | <tr><td>BackSeq-pGII</td><td><p class="text" style="text-transform:lowercase;">attaccggcctttgagtgagc</p></td></tr> | ||
</table> | </table> | ||
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Latest revision as of 23:36, 17 October 2014
Protocols / Primer
Here you find a list including the oligonucleotides used.
| Name | Sequence in 5' → 3' Direction |
| 01_pORE_E3_2x35S fwd | agcgtcggcgaggctaaaaagaacaaatgt |
| 02_pORE_E3_2x35S rev | ggaaGGATCCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAA |
| 03_Oligo1_RCF[21] | GATCGAATTCAGATCTGGATCCtaaCTCGAG |
| 04_Oligo2_RCF[21] | CGCGCTCGAGttaGGATCCAGATCTGAATTC |
| 05_F1_gfp_EMP | AGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTC |
| 06_R1_gfp_EMP | CCGGACTTGTCATCGTCGTCCTTGTAGTCTTTGTATAGTTCATCCATGCCATGTGTAATCCCAG |
| 07_R2_gfp_EMP | agcgtcggcgaggctaaaaagaacaaatgt |
| 08_TMBP_pASK_fwd | ggaaGAATTCgGACGAtGATGAtAAGGTGGTTATCAACGGCGTCAAGT |
| 09_TMBP_pASK_rev | ggaaCCATGGgtTTGTCgTCGTCaTCCTTACAGCTGCAAGATGATCCGC |
| 10_Expa4 in pSB1C3 fwd | aaGAATTCGCGGCCGCTTCTAGatggctattaaactagcaattc |
| 11_Expa4 in pSB1C3 rev | aaCTGCAGCGGCCGCTACTAGTAagcgtcggcgaggct |
| 12_TMBP in pSB1C3 fwd | aaGAATTCGCGGCCGCTTCTAGATGGTGGTTATCAACGGCGTCAAGT |
| 13_TMBP in pSB1C3 rev | aaCTGCAGCGGCCGCTACTAGTAttattaCTTACAGCTGCAAGATGATCCGC |
| 14_CBD in pSB1C3 fwd | aaGAATTCGCGGCCGCTTCTAGATGACCAGTTCTATGTCAGTAGAATTTT |
| 15_CBD in pSB1C3 rev | aaCTGCAGCGGCCGCTACTAGTAttattaaCGGCCTGACGCAAATCC |
| 16_VF2 | tgccacctgacgtctaagaa |
| 17_VR | attaccgcctttgagtgagc |
| T7 | CGACTCACTATAGGGCG |
| BackSeq-pGII | attaccggcctttgagtgagc |
