Team:CityU HK/project/result

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  <li><a href='https://2014.igem.org/Team:CityU_HK/project/result'><span>Result Summary</span></a></li>
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  <li><a href='https://2014.igem.org/Team:CityU_HK/project/result_fad'><span>FadD & FadL Module</span></a></li>
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  <li><a href='https://2014.igem.org/Team:CityU_HK/project/result_tesA'><span>'TesA Module</span></a></li>
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  <li class='last'><a href='https://2014.igem.org/Team:CityU_HK/project/result_desaturase'><span>Desaturase Module</span></a></li>
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<div><img src="https://static.igem.org/mediawiki/2014/b/bc/CityU_HK_result-banner.png" width="100%"></div>
<h1 id="title"> Result Summary </h1>
<h1 id="title"> Result Summary </h1>
<h2 class="sub">Experimental Achievements from 15 June – 3 October 2014 :</h2>
<h2 class="sub">Experimental Achievements from 15 June – 3 October 2014 :</h2>
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<p class="content">Successfully constructed a Biobrick for a tetracycline regulated fadL-fadD expression system (<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472606</a>).<br><br>
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Successfully constructed and characterized a T7-regulated fadL-fadD  system in the pSNAP-tag® (T7)-2 expression vector.<br><br>
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Successfully constructed a Biobrick containing a “leaderless” tesA coding sequence (‘tesA) (<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a>).<br><br>
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<p class="content">Successfully constructed a Biobrick for a tetracycline regulated <i>fadL-fadD</i> expression system (<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>).<br><br>
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Successfully constructed a translational unit of the ‘tesA gene in pAB1A2. <br><br>
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Successfully constructed and characterized a T7-regulated <i>fadL-fadD</i> system in the pSNAP-tag® (T7)-2 expression vector.<br><br>
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Successfully constructed an arabinose-regulated ‘tesA expression system in pSB1C3.<br><br>
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Successfully constructed a Biobrick containing a “leaderless” <i>tesA</i> coding sequence (<i>‘tesA</i>) (<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a>).<br><br>
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Successfully constructed a translational unit of the <i>‘tesA</i> gene in pAB1A2. <br><br>
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Successfully constructed an arabinose-regulated <i>‘tesA</i> expression system in pSB1C3.<br><br>
Successfully constructed a Biobrick containing a Δ15 desaturase gene that is codon-optimized for protein expression in E.coli (<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a>).<br><br>
Successfully constructed a Biobrick containing a Δ15 desaturase gene that is codon-optimized for protein expression in E.coli (<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a>).<br><br>
Successfully constructed a translational unit of a Δ9 desaturase gene in pSB1A2 that is codon-optimized for E. coli.<br><br>
Successfully constructed a translational unit of a Δ9 desaturase gene in pSB1A2 that is codon-optimized for E. coli.<br><br>
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Successfully constructed a translational unit of a Δ9-Δ12-Δ15 desaturase gene cluster in pSB1A2.
Successfully constructed a translational unit of a Δ9-Δ12-Δ15 desaturase gene cluster in pSB1A2.
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<!--==================== footer ===========================-->
 
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      <h3>Sponsors</h3>
 
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      <a href="http://www.cityu.edu.hk/" target="_blank"><img src="https://static.igem.org/mediawiki/2014/9/9c/CityU_HK_citylogo.png" width="95"></a>
 
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    <a href="http://www6.cityu.edu.hk/bhdbapp/deptweb/index.html" target="_blank"><img src="https://static.igem.org/mediawiki/2014/9/98/CityU_HK_bchlogo.png" width="80"></a>
 
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    <img src="https://static.igem.org/mediawiki/2014/a/aa/CityU_HK_Invitrogen_Logo.jpg" width="100">
 
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    <img src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_New_England_Biolabs_sponsor.png" width="135">
 
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    <img src="https://static.igem.org/mediawiki/2014/2/22/IDT-Logo.jpg" width="125">
 
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      <h3>Stay connected!</h3>
 
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      <p>email: <a href="#">cityuhk.igem@gmail.com</a></p>
 
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      <a href="https://www.facebook.com/igem.2014CityU"><img src="https://static.igem.org/mediawiki/2014/3/31/CityU_HK_fb_logo.png" width="50" height="48"></a>
 
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      <a href="https://twitter.com/CityUHK_iGEM"><img src="http://ibo2014.org/wordpress/wp-content/themes/ibo2014/img/navbar/twitter_icon.png" width="49" height="51"></a>
 
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<p>Copyright &copy; iGEM CityU HK 2014. All Rights Reserved</p>
 
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Latest revision as of 02:31, 17 October 2014

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Result Summary

Experimental Achievements from 15 June – 3 October 2014 :

Successfully constructed a Biobrick for a tetracycline regulated fadL-fadD expression system (BBa_K1472606).

Successfully constructed and characterized a T7-regulated fadL-fadD system in the pSNAP-tag® (T7)-2 expression vector.

Successfully constructed a Biobrick containing a “leaderless” tesA coding sequence (‘tesA) (BBa_K1472601).

Successfully constructed a translational unit of the ‘tesA gene in pAB1A2.

Successfully constructed an arabinose-regulated ‘tesA expression system in pSB1C3.

Successfully constructed a Biobrick containing a Δ15 desaturase gene that is codon-optimized for protein expression in E.coli (BBa_K1472610).

Successfully constructed a translational unit of a Δ9 desaturase gene in pSB1A2 that is codon-optimized for E. coli.

Successfully constructed a translational unit of a Δ12 desaturase gene in pSB1A2 that is codon-optimized for E. coli.

Successfully constructed a translational unit of a Δ15 desaturase gene in pSB1A2 that is codon-optimized for E. coli.

Successfully constructed a translational unit of a Δ9-Δ12 desaturase gene cluster in pSB1A2.

Successfully constructed a translational unit of a Δ9-Δ12-Δ15 desaturase gene cluster in pSB1A2.



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