Team:TU Eindhoven/Notebook

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<html>
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<title>TU Eindhoven iGEM Team</title>
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<link rel="stylesheet" type="text/css" href="https://2014.igem.org/Team:TU_Eindhoven/Notebook-css?action=raw&ctype=text/css"/>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:TU_Eindhoven/Notebook&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<h2>Notebook</h2>
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</div>
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</div>
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<div id="stickyheader" class="menu">
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      <div class="slider">
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        <ul>
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<li class="category active"><a href="#january">JANUARY</a></li>
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          <li class="category"><a href="#february">FEBRUARY</a></li>
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          <li class="category"><a href="#march">MARCH</a></li>
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          <li class="category"><a href="#april">APRIL</a></li>
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  <li class="category"><a href="#may">MAY</a></li>
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          <li class="category"><a href="#june">JUNE</a></li>
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          <li class="category"><a href="#july">JULY</a></li>
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          <li class="category"><a href="#august">AUGUST</a></li>
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          <li class="category"><a href="#september">SEPTEMBER</a></li>
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          <li class="category"><a href="#october">OCTOBER</a></li>
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    <div id="january" class="container january">
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      <h1 id="header1">January</h1>
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<p id="para">-Background research<br>
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</p>
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    </div>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<br><br>
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<a href="https://2014.igem.org/Team:TU_Eindhoven"style="color:#000000">Home </a> </td>
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    <div id="february" class="container february">
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<a href="https://2014.igem.org/Team:TU_Eindhoven/Team"style="color:#000000"> Team </a> </td>
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      <h1 id="header1">February</h1>
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<p id="para">-Background research<br>
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</p>
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    </div>
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<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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<br><br>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=TU_Eindhoven"style="color:#000000"> Official Team Profile </a></td>
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    <div id="march" class="container march">
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<a href="https://2014.igem.org/Team:TU_Eindhoven/Project"style="color:#000000"> Project</a></td>
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      <h1 id="header1">March</h1>
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<p id="para">
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-Selection of topic<br>
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-Further research<br>
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-Selection of projec<br>
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</p>
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    </div>
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<br><br>
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<a href="https://2014.igem.org/Team:TU_Eindhoven/Parts"style="color:#000000"> Parts</a></td>
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    <div id="april" class="container april">
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<a href="https://2014.igem.org/Team:TU_Eindhoven/Modeling"style="color:#000000"> Modeling</a></td>
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      <h1 id="header1">April</h1>
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<p id="para">-Further research <br>
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</p>
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    </div>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<br><br>
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<a href="https://2014.igem.org/Team:TU_Eindhoven/Notebook"style="color:#000000"> Notebook</a></td>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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    <div id="may" class="container may">
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<a href="https://2014.igem.org/Team:TU_Eindhoven/Safety"style=" color:#000000"> Safety </a></td>
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      <h1 id="header1">May</h1>
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<p id="para"><b><u>General Activities</b></u><br>
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Regular team meetings<br>
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Looking for sponsors<br>
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Month planning<br>
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</p>
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    </div>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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<br><br>
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<a href="https://2014.igem.org/Team:TU_Eindhoven/Attributions"style="color:#000000"> Attributions </a></td>
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    <div id="june" class="container june">
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      <h1 id="header1">June</h1>
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<p id="para"><b><u>Wetlab</u></b><br>
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- Arrival of the biobricks<br>
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- The designs for the plasmids are approved and ordered Primers<br>
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- Preparation of culture media, agar plates, antibiotics and glycerol<br>
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- Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX<br>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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    <div id="july" class="container july">
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      <h1 id="header1">July</h1>
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<p id="para"><b><u>Wetlab</b></u><br>
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<b>Week 27: 30 June – 6 July</b><br>
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Plasmid amplification  and culturing BBa_K811005(Penn) + CPX<br>
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Vector and insert Digestions<br>
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BBa_K811005(Penn) + CPX insert ligate pET29a <br>
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Colony PCR <br>
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<br>
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<b>Week 28: 7 July – 13 July</b><br>
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Colony PCR <br>
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Site directed mutagenesis of CPX <br>
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<br>
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<b>Week 29: 14 July – 20 July</b><br>
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Site directed mutagenesis of CPX <br>
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Sequencing of CPX and BBa_K811005(Penn)<br>
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<br>
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<b>Week 30: 21 July – 27 July</b><br>
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Sequencing results came in and were as expected<br>
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Protein expression <br>
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FACS – DBCO-PEG4-5/6-TAMRA <br>
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<br>
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<b>Week 31: 28 July – August 3</b><br>
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Protein expression<br>
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FACS – DBCO-PEG10kDa <br>
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</p>
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    </div>
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<br><br>
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    <div id="august" class="container august">
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      <h1 id="header1">August</h1>
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<p id="para"><b><u>Wetlab</b></u><br>
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<b>Week 32: 4 August – 10 August</b><br>
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Protein expression <br>
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FACS – Antibody titration <br>
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Protein expression curve <br>
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<br>
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<b>Week 33: 11 August – 17 August</b><br>
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FACS - DBCO PEG-5kDa fluorescent <br>
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<br>
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<b>Week 34: 18 August – 24 August</b><br>
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Start Bio-bricking<br>
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Start silencing mutagenesis <br>
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<br>
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<b>Week 35: 25 August – 31 August</b><br>
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</p>
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    </div>
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<br><br>
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    <div id="september" class="container september">
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      <h1 id="header1">September</h1>
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<p id="para"><b><u>Wetlab</b></u><br>
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<b>Week 36: 1 September – 7 September</b><br>
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<br>
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<b>Week 37: 8 September – 14 September</b><br>
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<br>
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<b>Week 38: 15 September – 21 September</b><br>
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<br>
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<b>Week 39: 22 September – 28 September</b><br>
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</p>
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    </div>
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<br><br>
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    <div id="october" class="container october">
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      <h1 id="header1">October</h1>
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<p id="para"><b><u>Wetlab</b></u><br>
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<b>Week 40: 29 September – 5 October</b><br>
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<br>
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<b>Week 41: 6 October – 12 October</b><br>
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<br>
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<b>Week 42: 13 October – 19 October</b><br>
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</p>
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Latest revision as of 12:58, 25 August 2014

TU Eindhoven iGEM Team

Notebook

January

-Background research



February

-Background research



March

-Selection of topic
-Further research
-Selection of projec



April

-Further research



May

General Activities
Regular team meetings
Looking for sponsors
Month planning



June

Wetlab
- Arrival of the biobricks
- The designs for the plasmids are approved and ordered Primers
- Preparation of culture media, agar plates, antibiotics and glycerol
- Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX



July

Wetlab
Week 27: 30 June – 6 July
Plasmid amplification and culturing BBa_K811005(Penn) + CPX
Vector and insert Digestions
BBa_K811005(Penn) + CPX insert ligate pET29a
Colony PCR

Week 28: 7 July – 13 July
Colony PCR
Site directed mutagenesis of CPX

Week 29: 14 July – 20 July
Site directed mutagenesis of CPX
Sequencing of CPX and BBa_K811005(Penn)

Week 30: 21 July – 27 July
Sequencing results came in and were as expected
Protein expression
FACS – DBCO-PEG4-5/6-TAMRA

Week 31: 28 July – August 3
Protein expression
FACS – DBCO-PEG10kDa



August

Wetlab
Week 32: 4 August – 10 August
Protein expression
FACS – Antibody titration
Protein expression curve

Week 33: 11 August – 17 August
FACS - DBCO PEG-5kDa fluorescent

Week 34: 18 August – 24 August
Start Bio-bricking
Start silencing mutagenesis

Week 35: 25 August – 31 August



September

Wetlab
Week 36: 1 September – 7 September

Week 37: 8 September – 14 September

Week 38: 15 September – 21 September

Week 39: 22 September – 28 September



October

Wetlab
Week 40: 29 September – 5 October

Week 41: 6 October – 12 October

Week 42: 13 October – 19 October



Created by iGEM Team TU Eindhoven 2014

Retrieved from "http://2014.igem.org/Team:TU_Eindhoven/Notebook"