Team:Tuebingen/Notebook/Protocols/gelextraction

From 2014.igem.org

(Difference between revisions)
(Created page with "{{:Team:Tuebingen/Templates/TueWikiBase}} <html> <head> </head> <body> <div id="TueContent"> <h3>Agarose gel electrophoresis</h3> </div> </body> </html>")
m
 
(One intermediate revision not shown)
Line 10: Line 10:
<div id="TueContent">
<div id="TueContent">
-
<h3>Agarose gel electrophoresis</h3>
+
<h1>Protocols</h1>
 +
 
 +
<h3>Agarose gel extraction</h3>
 +
 
 +
<h4>Procedure</h4>
 +
 
 +
<ol>
 +
  <li>Perform the gel extraction according to <a href="http://www.thermoscientificbio.com/uploadedFiles/Resources/k069-product-information.pdf">Thermo Scientific GeneJET Gel Extraction Kit Manual</a>. </li>
 +
 
 +
  <li>Elute in 30 μl buffer </li>
 +
 
 +
  <li>Run eluate twice through the column in order to increase DNA yields </li>
 +
 
 +
  <li>Perform gel electrophoresis to control the gel extraction. </li>
 +
 
 +
  <li>Store at -20 °C  </li>
 +
</ol>
 +
 
 +
 
</div>
</div>

Latest revision as of 15:25, 17 October 2014


Protocols

Agarose gel extraction

Procedure

  1. Perform the gel extraction according to Thermo Scientific GeneJET Gel Extraction Kit Manual.
  2. Elute in 30 μl buffer
  3. Run eluate twice through the column in order to increase DNA yields
  4. Perform gel electrophoresis to control the gel extraction.
  5. Store at -20 °C

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/gelextraction"