Team:CSU Fort Collins/Notebook/HVP/Jun
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- | <div id='banner'><center><img src='https://static.igem.org/mediawiki/2014/b/b2/Team-CSU_Notebook.jpg' style='width:100%; margin-top:-300px'/> | + | <div id='banner'><center><img src='https://static.igem.org/mediawiki/2014/b/b2/Team-CSU_Notebook.jpg' style='width:100%; margin-top:-300px'/><a href='https://2014.igem.org'><img src='https://static.igem.org/mediawiki/2014/d/d7/Sysuchina_igemdeLogo.png' style="width:80px; position:absolute; top: 10px; right: 20px; opacity:0.95;"/></a> |
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<div id='cssmenu'> | <div id='cssmenu'> | ||
<ul> | <ul> | ||
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<ul> | <ul> | ||
<li><a href='/Team:CSU_Fort_Collins/Members/'><span>Members</span></a></li> | <li><a href='/Team:CSU_Fort_Collins/Members/'><span>Members</span></a></li> | ||
- | <li class='last'><a href='/Team:CSU_Fort_Collins/ | + | <li><a href='/Team:CSU_Fort_Collins/Sponsors/'><span>Sponsors</span></a></li> |
+ | <li class='last'><a href='/Team:CSU_Fort_Collins/Acknowledgements/'><span>Acknowledgements</span></a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
- | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/'><span>Breakdown</span></a> | + | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul'><span>Breakdown</span></a> |
<ul> | <ul> | ||
- | <li><a href=" | + | <li><a href="/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul"><span>July</span></a></li> |
- | <li><a href=' | + | <li><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Aug'><span>August</span></a></li> |
- | <li class='last'><a href=" | + | <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/Breakdown/Sep"><span>September</span></a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
- | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/HVP/'><span>High-Value Product</span></a> | + | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/HVP/Jun'><span>High-Value Product</span></a> |
<ul> | <ul> | ||
- | <li><a href=" | + | <li><a href="/Team:CSU_Fort_Collins/Notebook/HVP/Jun"><span>June</span></a></li> |
- | + | <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/HVP/Jul"><span>July</span></a></li> | |
- | + | ||
- | <li class='last'><a href=" | + | |
</ul> | </ul> | ||
</li> | </li> | ||
- | <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/'><span>Kill Switch</span></a> | + | <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/Jul'><span>Kill Switch</span></a> |
<ul> | <ul> | ||
- | <li><a href=" | + | <li><a href="/Team:CSU_Fort_Collins/Notebook/KillSwitch/Jul"><span>July</span></a></li> |
- | + | <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/KillSwitch/Sep"><span>September</span></a></li> | |
- | <li class='last'><a href=" | + | |
</ul> | </ul> | ||
</li> | </li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
- | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/ | + | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Collab/'><span>Human Practices</span></a> |
<ul> | <ul> | ||
<li><a href='/Team:CSU_Fort_Collins/Collab/'><span>Collaboration</span></a></li> | <li><a href='/Team:CSU_Fort_Collins/Collab/'><span>Collaboration</span></a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
- | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/ | + | <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Parts/'><span>Achievements</span></a> |
<ul> | <ul> | ||
<li><a href='/Team:CSU_Fort_Collins/Parts/'><span>Parts</span></a></li> | <li><a href='/Team:CSU_Fort_Collins/Parts/'><span>Parts</span></a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
- | <li><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li> | + | <li class='last'><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="month-group"> | <div class="month-group"> | ||
<a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jun'>Jun</a> | <a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jun'>Jun</a> | ||
+ | </div> | ||
<p>pMBI Assembly</p> | <p>pMBI Assembly</p> | ||
- | |||
<div class="month-group"> | <div class="month-group"> | ||
<a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul'>July</a> | <a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul'>July</a> | ||
+ | </div> | ||
<p>pMBI and pMevT Assembly</p> | <p>pMBI and pMevT Assembly</p> | ||
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</div> | </div> | ||
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<h4>Monday, June 9 - Monday, June 16</h4> | <h4>Monday, June 9 - Monday, June 16</h4> | ||
- | Researched the mevalonate pathway in E. coli and sequenced the genes to be inserted into puc19. Designed primers as specified in the <a href='/Team:CSU_Fort_Collins/Notebook/Protocols=Gibson'>Gibson Assembly Protocol</a> for transformation of the mevalonate pathway from yeast into <u>E. coli</u>. | + | Researched the mevalonate pathway in <u>E. coli</u> and sequenced the genes to be inserted into puc19. Designed primers as specified in the <a href='/Team:CSU_Fort_Collins/Notebook/Protocols=Gibson'>Gibson Assembly Protocol</a> for transformation of the mevalonate pathway from yeast into <u>E. coli</u>. |
<h4>Thursday, June 19</h4> | <h4>Thursday, June 19</h4> | ||
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<h4>Wednesday, June 25</h4> | <h4>Wednesday, June 25</h4> | ||
- | Took a small sample of E. coli with puc19 from a previous colony and suspended in LB Broth. Shook at 225 rpm in incubator at 37° C overnight to culture. | + | Took a small sample of <u>E. coli</u> with puc19 from a previous colony and suspended in LB Broth. Shook at 225 rpm in incubator at 37° C overnight to culture. |
<h4>Thursday, June 26</h4> | <h4>Thursday, June 26</h4> | ||
- | Completed a miniprep of E. coli with puc19 from the overnight culture. Followed <a href='/Team:CSU_Fort_Collins/Notebook/Protocol/Miniprep'>plasmid miniprep protocol</a> as given in the Informatics (Life Technologies) kit. | + | Completed a miniprep of <u>E. coli</u> with puc19 from the overnight culture. Followed <a href='/Team:CSU_Fort_Collins/Notebook/Protocol/Miniprep'>plasmid miniprep protocol</a> as given in the Informatics (Life Technologies) kit. |
<h4>Friday, June 27</h4> | <h4>Friday, June 27</h4> |
Latest revision as of 20:17, 17 October 2014
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