Team:Aachen/Notebook/Wetlab/April
From 2014.igem.org
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<ul class="menusmall-grid"> | <ul class="menusmall-grid"> | ||
- | |||
- | + | <!-- <li style="width:106px;margin-left: 12px;margin-right: 12px;" > | |
- | <a class="menulink" href="https://2014.igem.org/Team:Aachen/ | + | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/March" style="color:black"> |
- | <div class="menusmall-item menusmall-info" ><div class="menukachel" | + | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to March</div></div> |
- | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/ | + | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/7/7a/Aachen_14-10-10_March_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> |
+ | </div> | ||
+ | </a> | ||
+ | </li> --> | ||
+ | |||
+ | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > | ||
+ | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/April" style="color:black"> | ||
+ | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to April</div></div> | ||
+ | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/2/2d/Aachen_14-10-10_April_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> | ||
</div> | </div> | ||
</a> | </a> | ||
</li> | </li> | ||
- | <li> | + | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > |
- | <a class="menulink" href="https://2014.igem.org/Team:Aachen/ | + | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/May" style="color:black"> |
- | <div class="menusmall-item menusmall-info" ><div class="menukachel"> | + | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to May</div></div> |
- | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/ | + | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/67/Aachen_14-10-10_May_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> |
</div> | </div> | ||
</a> | </a> | ||
</li> | </li> | ||
- | <li> | + | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > |
- | <a class="menulink" href="https://2014.igem.org/Team:Aachen/ | + | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/June" style="color:black"> |
- | <div class="menusmall-item menusmall-info" ><div class="menukachel"> | + | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to June</div></div> |
- | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/ | + | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/1/1d/Aachen_14-10-10_June_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> |
</div> | </div> | ||
</a> | </a> | ||
</li> | </li> | ||
- | <li> | + | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > |
- | <a class="menulink" href="https://2014.igem.org/Team:Aachen/ | + | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/July" style="color:black"> |
- | <div class="menusmall-item menusmall-info" ><div class="menukachel"> | + | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to July</div></div> |
- | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/ | + | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/1/19/Aachen_14-10-10_July_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> |
</div> | </div> | ||
</a> | </a> | ||
</li> | </li> | ||
- | + | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > | |
- | <a class="menulink" href="https://2014.igem.org/Team:Aachen/ | + | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/August" style="color:black"> |
- | <div class="menusmall-item menusmall-info" ><div class="menukachel"> | + | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to August</div></div> |
- | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/ | + | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/4/4e/Aachen_14-10-10_August_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> |
</div> | </div> | ||
</a> | </a> | ||
</li> | </li> | ||
- | </ | + | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > |
- | </ | + | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/September" style="color:black"> |
- | </ | + | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to September</div></div> |
+ | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/d/d4/Aachen_14-10-10_September_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> | ||
+ | </div> | ||
+ | </a> | ||
+ | </li> | ||
+ | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > | ||
+ | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/October" style="color:black"> | ||
+ | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to October</div></div> | ||
+ | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/60/Aachen_14-10-10_October_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> | ||
+ | </div> | ||
+ | </a> | ||
+ | </li> | ||
- | < | + | </ul> |
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</center> | </center> | ||
+ | </html> | ||
= April = | = April = | ||
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'''Organization''' | '''Organization''' | ||
- | + | Getting all kind of lab equipment | |
* key cards and keys for the lab | * key cards and keys for the lab | ||
* cooled centifuge | * cooled centifuge | ||
- | * space in - | + | * space reservation in -80°C freezer |
- | * bottles for 70 | + | * bottles for 70% ethanol |
* ... (problems of a first year team ^^) | * ... (problems of a first year team ^^) | ||
== 12th == | == 12th == | ||
- | * | + | * chemically competent ''E. coli'' NEB Top10, DH5α and BL21 were made |
== 15th == | == 15th == | ||
* 800 ml LB for plates with 1.5% agar and kanamycin (50 µg/L) were prepared | * 800 ml LB for plates with 1.5% agar and kanamycin (50 µg/L) were prepared | ||
- | * | + | * test of transformation efficiency of our competent cells |
** Colonies on the agar plates were counted after transformation with 1 µl DNA (147 ng/µl) | ** Colonies on the agar plates were counted after transformation with 1 µl DNA (147 ng/µl) | ||
** Cells had been incubated in SOC or LB medium | ** Cells had been incubated in SOC or LB medium | ||
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| '''DH5α''' || SOC: 30 LB: 10 ||SOC: 7 LB: 1 || SOC: 1 LB: 2 || SOC: 2 LB: 1 || | | '''DH5α''' || SOC: 30 LB: 10 ||SOC: 7 LB: 1 || SOC: 1 LB: 2 || SOC: 2 LB: 1 || | ||
|- | |- | ||
- | | '''NEB | + | | '''NEB 10β''' || SOC: 170 LB: 135 ||SOC: 100 LB: 79 || SOC: 25 LB: 22 || SOC: 9 LB: 9 || SOC: 1 LB:0 |
|- | |- | ||
|} | |} | ||
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* BL21 cells were centrifuged and plated; only 128 colonies grew in total indicating a very bad efficiency (~482.11). | * BL21 cells were centrifuged and plated; only 128 colonies grew in total indicating a very bad efficiency (~482.11). | ||
- | Following formula was used to calculate the efficiency: | + | Following formula was used to calculate the efficiency: ''Efficiency = (CFU /µg DNA) / Dilution'' |
- | + | ||
* for DH5α: medial efficiency in SOC: 1864.36, and in LB: 440.68 | * for DH5α: medial efficiency in SOC: 1864.36, and in LB: 440.68 | ||
* for NEB: medial efficiency in SOC: 6779.66, and in LB: 4698.30 | * for NEB: medial efficiency in SOC: 6779.66, and in LB: 4698.30 | ||
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'''Pcq lab PCR''' advanced primus 25, 96 | '''Pcq lab PCR''' advanced primus 25, 96 | ||
- | < | + | <div style="float:left;margin-right:1em;"> |
{| class="wikitable" | {| class="wikitable" | ||
! !! Lenght [bp] !! % GC !! T<sub>m</sub> !! T<sub>A</sub> | ! !! Lenght [bp] !! % GC !! T<sub>m</sub> !! T<sub>A</sub> | ||
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| '''REACh2_N''' || 320 || 59 || 82 || 54 | | '''REACh2_N''' || 320 || 59 || 82 || 54 | ||
|- | |- | ||
- | |} | + | |} </div> |
- | </ | + | <div style="float:left;margin-right:1em;"> |
- | + | ||
- | + | ||
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- | < | + | |
{| class="wikitable" | {| class="wikitable" | ||
! Parameter !! Duration !! Temp [°C] | ! Parameter !! Duration !! Temp [°C] | ||
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|- | |- | ||
| Store||forever||8 | | Store||forever||8 | ||
- | |} | + | |} </div> |
- | </ | + | |
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# RFC25_F RFC25_R | # RFC25_F RFC25_R | ||
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</center> | </center> | ||
- | '''Anneling''': | + | '''Anneling''': 52°C |
'''Elongation''': 20 sec | '''Elongation''': 20 sec | ||
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* '''SOE2''' | * '''SOE2''' | ||
- | + | <html> | |
<center> | <center> | ||
- | + | <ul class="menusmall-grid"> | |
- | + | ||
- | + | <!-- <li style="width:106px;margin-left: 12px;margin-right: 12px;" > | |
+ | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/March" style="color:black"> | ||
+ | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to March</div></div> | ||
+ | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/7/7a/Aachen_14-10-10_March_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> | ||
+ | </div> | ||
+ | </a> | ||
+ | </li> --> | ||
+ | |||
+ | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > | ||
+ | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/April" style="color:black"> | ||
+ | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to April</div></div> | ||
+ | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/2/2d/Aachen_14-10-10_April_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> | ||
+ | </div> | ||
+ | </a> | ||
+ | </li> | ||
+ | |||
+ | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > | ||
+ | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/May" style="color:black"> | ||
+ | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to May</div></div> | ||
+ | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/67/Aachen_14-10-10_May_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> | ||
+ | </div> | ||
+ | </a> | ||
+ | </li> | ||
+ | |||
+ | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > | ||
+ | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/June" style="color:black"> | ||
+ | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to June</div></div> | ||
+ | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/1/1d/Aachen_14-10-10_June_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> | ||
+ | </div> | ||
+ | </a> | ||
+ | </li> | ||
+ | |||
+ | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > | ||
+ | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/July" style="color:black"> | ||
+ | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to July</div></div> | ||
+ | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/1/19/Aachen_14-10-10_July_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> | ||
+ | </div> | ||
+ | </a> | ||
+ | </li> | ||
+ | |||
+ | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > | ||
+ | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/August" style="color:black"> | ||
+ | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to August</div></div> | ||
+ | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/4/4e/Aachen_14-10-10_August_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> | ||
+ | </div> | ||
+ | </a> | ||
+ | </li> | ||
+ | |||
+ | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > | ||
+ | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/September" style="color:black"> | ||
+ | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to September</div></div> | ||
+ | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/d/d4/Aachen_14-10-10_September_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> | ||
+ | </div> | ||
+ | </a> | ||
+ | </li> | ||
+ | |||
+ | <li style="width:106px;margin-left: 12px;margin-right: 12px;" > | ||
+ | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/October" style="color:black"> | ||
+ | <div class="menusmall-item menusmall-info" style="height:100px; width: 100px;" ><div class="menukachel" style="top: 30%; font-size: 16px;">Go to October</div></div> | ||
+ | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/60/Aachen_14-10-10_October_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%; height:100px; width: 100px;"> | ||
+ | </div> | ||
+ | </a> | ||
+ | </li> | ||
+ | |||
+ | </ul> | ||
</center> | </center> | ||
+ | </html> | ||
{{Team:Aachen/Footer}} | {{Team:Aachen/Footer}} |
Latest revision as of 17:53, 17 October 2014
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