Team:HZAU-China/Output

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             <a href="https://igem.org/Team.cgi?year=2014&team_name=HZAU-China"><img src="https://static.igem.org/mediawiki/2014/b/bb/Hazuteamlogo918.png" alt="HZAU-China" /></a>
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             <a href="https://igem.org/Team.cgi?year=2014&team_name=HZAU-China"><img src="https://static.igem.org/mediawiki/2014/4/4c/Hzau-home-lllogo.png" alt="HZAU-China" /></a>
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                 <li class="selected dropdown"><a href="https://2014.igem.org/Team:HZAU-China/Project">Project</a>
                 <li class="selected dropdown"><a href="https://2014.igem.org/Team:HZAU-China/Project">Project</a>
    <ul>  
    <ul>  
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Project"><span>-</span>Background</a></li>
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Design"><span>-</span>Overview</a></li>
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<li><a href="https://2014.igem.org/Team:HZAU-China/Design"><span>-</span>Design overview</a></li>
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                        <li><a href="https://2014.igem.org/Team:HZAU-China/Background"><span>-</span>Background</a></li>
<li><a href="https://2014.igem.org/Team:HZAU-China/Input"><span>-</span>Input module</a></li>
<li><a href="https://2014.igem.org/Team:HZAU-China/Input"><span>-</span>Input module</a></li>
<li><a href="https://2014.igem.org/Team:HZAU-China/Processing"><span>-</span>Processing module</a></li>
<li><a href="https://2014.igem.org/Team:HZAU-China/Processing"><span>-</span>Processing module</a></li>
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<li class="dropdown"><a href="https://2014.igem.org/Team:HZAU-China/Review">Wetlab</a>
<li class="dropdown"><a href="https://2014.igem.org/Team:HZAU-China/Review">Wetlab</a>
                     <ul>  
                     <ul>  
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Review"><span>-</span>Overview</a></li>
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Overview"><span>-</span>Overview</a></li>
<li><a href="https://2014.igem.org/Team:HZAU-China/Construction"><span>-</span>Construction</a></li>
<li><a href="https://2014.igem.org/Team:HZAU-China/Construction"><span>-</span>Construction</a></li>
<li><a href="https://2014.igem.org/Team:HZAU-China/Characterization"><span>-</span>Characterization</a></li>
<li><a href="https://2014.igem.org/Team:HZAU-China/Characterization"><span>-</span>Characterization</a></li>
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                        <li><a href="https://2014.igem.org/Team:HZAU-China/Help"><span>-</span>Help each other</a></li>
                         <li><a href="https://2014.igem.org/Team:HZAU-China/Protocol"><span>-</span>Protocol</a></li>
                         <li><a href="https://2014.igem.org/Team:HZAU-China/Protocol"><span>-</span>Protocol</a></li>
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Labnotes"><span>-</span>Labnotes</a></li>  
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Labnotes"><span>-</span>Labnotes</a></li>      
                     </ul>
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                 <li class="dropdown"><a href="https://2014.igem.org/Team:HZAU-China/Modeling">Modeling</a>
                 <li class="dropdown"><a href="https://2014.igem.org/Team:HZAU-China/Modeling">Modeling</a>
                     <ul>  
                     <ul>  
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Modeling"><span>-</span>Overview</a></li>
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/MOverview"><span>-</span>Overview</a></li>
                         <li><a href="https://2014.igem.org/Team:HZAU-China/Biological"><span>-</span>Biological processes</a></li>
                         <li><a href="https://2014.igem.org/Team:HZAU-China/Biological"><span>-</span>Biological processes</a></li>
                         <li><a href="https://2014.igem.org/Team:HZAU-China/Comparison"><span>-</span>Comparison between different designs</a></li>
                         <li><a href="https://2014.igem.org/Team:HZAU-China/Comparison"><span>-</span>Comparison between different designs</a></li>
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<li class="dropdown"><a href="https://2014.igem.org/Team:HZAU-China/Achievements">Achievements</a>
<li class="dropdown"><a href="https://2014.igem.org/Team:HZAU-China/Achievements">Achievements</a>
    <ul>                         
    <ul>                         
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Achievements"><span>-</span>Judging</a></li>
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Achievements"><span>-</span>Judgement</a></li>
<li><a href="https://2014.igem.org/Team:HZAU-China/Parts"><span>-</span>Parts</a></li>
<li><a href="https://2014.igem.org/Team:HZAU-China/Parts"><span>-</span>Parts</a></li>
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<li><a href="https://2014.igem.org/Team:HZAU-China/Collaboration"><span>-</span>Collaboration</a></li>
<li><a href="https://2014.igem.org/Team:HZAU-China/Collaboration"><span>-</span>Collaboration</a></li>
                         <li><a href="https://2014.igem.org/Team:HZAU-China/Meetup"><span>-</span>Meetup</a></li>
                         <li><a href="https://2014.igem.org/Team:HZAU-China/Meetup"><span>-</span>Meetup</a></li>
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Humanities"><span>-</span>Humanities and Aesthetics</a></li>
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Humanities"><span>-</span>Humanities</a></li>
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Economics"><span>-</span>Economics and Law</a></li>
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Economics"><span>-</span>Social Sciences</a></li>
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Pubic outreach"><span>-</span>Pubic outreach</a></li>
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                         <li><a href="https://2014.igem.org/Team:HZAU-China/Pubic outreach"><span>-</span>Public outreach</a></li>
                         <li><a href="https://2014.igem.org/Team:HZAU-China/Safety"><span>-</span>Safety</a></li>
                         <li><a href="https://2014.igem.org/Team:HZAU-China/Safety"><span>-</span>Safety</a></li>
                         <li><a href="https://2014.igem.org/Team:HZAU-China/Social media"><span>-</span>Social media</a></li>   
                         <li><a href="https://2014.igem.org/Team:HZAU-China/Social media"><span>-</span>Social media</a></li>   
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             <li>Project</li>
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    <li>Output module</li>
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<h3 style="text-align:center">Output Module</h3> 
               <p class="highlighttext">In the first stage, the output module is the fluorescent indicator that can monitor the real-time processes of our system. Once its function is confirmed, we can use other functional parts as output to solve real world problems.</p>
               <p class="highlighttext">In the first stage, the output module is the fluorescent indicator that can monitor the real-time processes of our system. Once its function is confirmed, we can use other functional parts as output to solve real world problems.</p>
<h5>RNA level fluorescence</h5>
<h5>RNA level fluorescence</h5>
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               <p class="highlighttext">Beside fluorescent proteins, we try to use an RNA-fluorophore complex (Paige et al., 2011) to monitor the real-time processes. The complex contains RNA aptamers and some corresponding fluorophores. We synthesized the fluorophore 3,5-dimethoxy-4- hydroxybenzylidene imidazolinone (DHMBI), because several aptamers were identified that exhibited markedly different spectral properties when they bound to DHMBI. We also synthesized the 13-2min sequence, one of the aptamers that can interact with DHMBI, with a modified tRNA scaffold, which can stabilized the structure.</p>
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               <p class="highlighttext">Beside fluorescent proteins, we try to use an RNA-fluorophore complex (Paige <span style="font-style:italic;">et al.</span>, 2011) to monitor the real-time processes. The complex contains RNA aptamers and some corresponding fluorophores. We synthesized the fluorophore 3,5-dimethoxy-4-hydroxybenzylidene imidazolinone (DHMBI), because several aptamers were identified that exhibited markedly different spectral properties when they bound to DHMBI. We also synthesized the 13-2min sequence, one of the aptamers that can interact with DHMBI, with a modified tRNA scaffold, which can stabilize the structure.</p>
<img src="https://static.igem.org/mediawiki/2014/d/d4/Hzau-project-5_meitu_1.jpg"  width="600px" class="img-center"/>
<img src="https://static.igem.org/mediawiki/2014/d/d4/Hzau-project-5_meitu_1.jpg"  width="600px" class="img-center"/>
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<p class="figuretext">Figure 1: our resulting image under the fluorescent microscope</p>
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<p class="figuretext">Figure 1. The resulting image under the fluorescent microscope.</p>
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<h5>References</h5>
 
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<p class="highlighttext">Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642-646.</p>
 
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<h5>References</h5>
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<p class="highlighttext">Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642-646.</p>
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Latest revision as of 03:36, 18 October 2014

<!DOCTYPE html> 2014HZAU-China

Output module

Output Module

In the first stage, the output module is the fluorescent indicator that can monitor the real-time processes of our system. Once its function is confirmed, we can use other functional parts as output to solve real world problems.

RNA level fluorescence

Beside fluorescent proteins, we try to use an RNA-fluorophore complex (Paige et al., 2011) to monitor the real-time processes. The complex contains RNA aptamers and some corresponding fluorophores. We synthesized the fluorophore 3,5-dimethoxy-4-hydroxybenzylidene imidazolinone (DHMBI), because several aptamers were identified that exhibited markedly different spectral properties when they bound to DHMBI. We also synthesized the 13-2min sequence, one of the aptamers that can interact with DHMBI, with a modified tRNA scaffold, which can stabilize the structure.

Figure 1. The resulting image under the fluorescent microscope.

References

Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642-646.

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