Team:CSU Fort Collins/Notebook/Biosensor/Jul

From 2014.igem.org

(Difference between revisions)
 
(3 intermediate revisions not shown)
Line 590: Line 590:
.page a{
.page a{
     border-bottom: dotted 2px;
     border-bottom: dotted 2px;
 +
}
 +
#banner a:hover{
 +
opacity:0.8;
}
}
Line 600: Line 603:
<body style='margin:0'>
<body style='margin:0'>
-
<div id='banner'><center><img src='http://oi57.tinypic.com/1498eg5.jpg' style='width:100%; margin-top:-200px'/>
+
<div id='banner'><center><img src='https://static.igem.org/mediawiki/2014/b/b2/Team-CSU_Notebook.jpg' style='width:100%; margin-top:-300px'/><a href='https://2014.igem.org/Main_Page'><img src='https://static.igem.org/mediawiki/2014/d/d7/Sysuchina_igemdeLogo.png' style="width:80px; position:absolute; top: 10px; right: 20px; opacity:0.95;"/></a>
</center></div>
</center></div>
Line 617: Line 620:
       <ul>
       <ul>
         <li><a href='/Team:CSU_Fort_Collins/Members/'><span>Members</span></a></li>
         <li><a href='/Team:CSU_Fort_Collins/Members/'><span>Members</span></a></li>
-
         <li class='last'><a href='/Team:CSU_Fort_Collins/Sponsors/'><span>Sponsors</span></a></li>
+
        <li><a href='/Team:CSU_Fort_Collins/Sponsors/'><span>Sponsors</span></a></li>
 +
         <li class='last'><a href='/Team:CSU_Fort_Collins/Acknowledgements/'><span>Acknowledgements</span></a></li>
       </ul>
       </ul>
   </li>
   </li>
Line 634: Line 638:
       <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/DailyNotes'><span>Daily Notes</span></a>
       <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/DailyNotes'><span>Daily Notes</span></a>
         <ul>
         <ul>
-
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/'><span>Biosensor</span></a>
+
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Jun'><span>Biosensor</span></a>
             <ul>
             <ul>
-
               <li><a href="#"><span>June</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/Biosensor/Jun"><span>June</span></a></li>
-
               <li><a href="#"><span>July</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/Biosensor/Jul"><span>July</span></a></li>
-
              <li><a href='#'><span>August</span></a></li>
+
               <li class='last'><a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Aug'><span>August</span></a></li>
-
               <li class='last'><a href="#"><span>September</span></a></li>
+
             </ul>
             </ul>
         </li>
         </li>
-
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/'><span>Breakdown</span></a>
+
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul'><span>Breakdown</span></a>
             <ul>
             <ul>
-
               <li><a href="#"><span>July</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/Breakdown/Jul"><span>July</span></a></li>
-
               <li><a href='#'><span>August</span></a></li>
+
               <li><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/Aug'><span>August</span></a></li>
-
               <li class='last'><a href="#"><span>September</span></a></li>
+
               <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/Breakdown/Sep"><span>September</span></a></li>
             </ul>
             </ul>
         </li>
         </li>
-
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/HVP/'><span>High-Value Product</span></a>
+
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/HVP/Jun'><span>High-Value Product</span></a>
             <ul>
             <ul>
-
               <li><a href="#"><span>June</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/HVP/Jun"><span>June</span></a></li>
-
              <li><a href="#"><span>July</span></a></li>
+
               <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/HVP/Jul"><span>July</span></a></li>
-
              <li><a href='#'><span>August</span></a></li>
+
-
               <li class='last'><a href="#"><span>September</span></a></li>
+
             </ul>
             </ul>
         </li>
         </li>
-
         <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/'><span>Kill Switch</span></a>
+
         <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/Jul'><span>Kill Switch</span></a>
             <ul>
             <ul>
-
               <li><a href="#"><span>July</span></a></li>
+
               <li><a href="/Team:CSU_Fort_Collins/Notebook/KillSwitch/Jul"><span>July</span></a></li>
-
              <li><a href='#'><span>August</span></a></li>
+
               <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/KillSwitch/Sep"><span>September</span></a></li>
-
               <li class='last'><a href="#"><span>September</span></a></li>
+
             </ul>
             </ul>
         </li>
         </li>
Line 668: Line 668:
   </ul>
   </ul>
   </li>
   </li>
-
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/HumPrac/'><span>Human Practices</span></a>
+
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Collab/'><span>Human Practices</span></a>
       <ul>
       <ul>
         <li><a href='/Team:CSU_Fort_Collins/Collab/'><span>Collaboration</span></a></li>
         <li><a href='/Team:CSU_Fort_Collins/Collab/'><span>Collaboration</span></a></li>
Line 674: Line 674:
       </ul>
       </ul>
   </li>
   </li>
-
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Achievements/'><span>Achievements</span></a>
+
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Parts/'><span>Achievements</span></a>
       <ul>
       <ul>
         <li><a href='/Team:CSU_Fort_Collins/Parts/'><span>Parts</span></a></li>
         <li><a href='/Team:CSU_Fort_Collins/Parts/'><span>Parts</span></a></li>
Line 680: Line 680:
       </ul>
       </ul>
   </li>
   </li>
-
   <li><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li>
+
   <li class='last'><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li>
</ul>
</ul>
</div>
</div>
Line 697: Line 697:
       <div class="month-group">
       <div class="month-group">
         <a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Jun'>June</a>
         <a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Jun'>June</a>
-
        <p>PAR, PLR, and PFL Assembly</p>
 
       </div>
       </div>
 +
        <p>PAR, PLR, and PFL Assembly</p>
       <div class="month-group">
       <div class="month-group">
         <a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Jul'>July</a>
         <a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Jul'>July</a>
-
        <p>PLR, PFL, and PFL2 Assembly</p>
 
       </div>
       </div>
 +
        <p>PLR, PFL, and PFL2 Assembly</p>
       <div class="month-group">
       <div class="month-group">
         <a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Aug'>August</a>
         <a href='/Team:CSU_Fort_Collins/Notebook/Biosensor/Aug'>August</a>
 +
</div>
         <p>Scientists vs. the PCR Machine</p>
         <p>Scientists vs. the PCR Machine</p>
-
      </div>
 
   </div>
   </div>
 +
   <div class='page'>
   <div class='page'>

Latest revision as of 20:14, 17 October 2014

Biosensor Notes - July

Biosensor Daily Notes

Show Table of Contents
Hide Table of Contents


← Full Table of Contents
June

PAR, PLR, and PFL Assembly

July

PLR, PFL, and PFL2 Assembly

August

Scientists vs. the PCR Machine

JULY

Monday, June 30 - Friday, July 4

Assembled PFL 2 promoter and amplified via PCR. Ligated PLR promoter and attempted transformation with PAR promoter. Plated PAR promoter containing competent cells, but nothing grew. Plated again and still nothing grew. Ran restriction enzyme digest for PFL promoter. Ran ligation on PFL promoter and restriction enzyme digest on PFL2 promoter.

Monday, July 7 - Friday, July 11

Ligated for PFL 2 promoter. Ran a transformation for all four promoters; PLR, PAR, PFL, PFL2. Plates did not show sign of growth until Saturday.

Monday, July 14 - Friday, July 18

PLR, PFL, and PFL2 had cultures grow on their respective plates. PAR had no cultures grow. Made overnight cultures from PFL, PFL2, PLR and ran a colony PCR from the same samples we used for the overnight cultures. Ran our PCR products through a 0.7% agarose gel and no bands were present. Ran another colony PCR with PFL and PLR while incubating PFL2 to allow it to grow. Gel with PFL and PLR showed no bands.

Monday, July 21 - Friday, July 25

Miniprepped 4 samples; two from PFL and two from PLR. Amplified extracted DNA via PCR. Gel with PCR products showed no bands. Made a new set of overnight cultures from the same plates as before, but different colonies.

Monday, July 28 - Friday, August 1

Ran a restriction enzyme digest on Savannah's miniprep products. Made 1X TAE. Ran a gel with restriction enzyme digest products, no bands appeared. Nanodropped our miniprep results to determine concentration of DNA. Selected one sample from each promoter that had an adequate amount of DNA and ran a gel, which showed no bands. Plated PAR in order to culture cells, which plates showed no sign of later.



Previous Next