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Team:Berlin/Project
From 2014.igem.org
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<ul class="main-menue-ul"> | <ul class="main-menue-ul"> | ||
<a href="https://2014.igem.org/Team:Berlin" class="main-menue-links"><li>Home</li></a> | <a href="https://2014.igem.org/Team:Berlin" class="main-menue-links"><li>Home</li></a> | ||
| - | <a href="https://2014.igem.org/Team:Berlin/Project" class="main-menue-links"><li>Project</li></a> | + | <a href="https://2014.igem.org/Team:Berlin/Project" class="main-menue-links"><li class="active">Project</li></a> |
<a href="https://2014.igem.org/Team:Berlin/Team" class="main-menue-links"><li>Team</li></a> | <a href="https://2014.igem.org/Team:Berlin/Team" class="main-menue-links"><li>Team</li></a> | ||
<a href="https://2014.igem.org/Team:Berlin/Safety" class="main-menue-links"><li>Safety</li></a> | <a href="https://2014.igem.org/Team:Berlin/Safety" class="main-menue-links"><li>Safety</li></a> | ||
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<a href="https://2014.igem.org/Team:Berlin/Workshop" class="main-menue-links"><li>Workshop</li></a> | <a href="https://2014.igem.org/Team:Berlin/Workshop" class="main-menue-links"><li>Workshop</li></a> | ||
| - | <a href="https://2014.igem.org/Team:Berlin/Blog"><li>Blog</li></a> | + | <a href="https://2014.igem.org/Team:Berlin/Blog" class="main-menue-links"><li>Blog</li></a> |
<a href="https://igem.org/Main_Page" class="igem-link"><li><img class="igem-logo" src="https://static.igem.org/mediawiki/2014/d/d8/Team_Berlin_igem_logo.png"></li></a> | <a href="https://igem.org/Main_Page" class="igem-link"><li><img class="igem-logo" src="https://static.igem.org/mediawiki/2014/d/d8/Team_Berlin_igem_logo.png"></li></a> | ||
</ul> | </ul> | ||
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<li><a href="https://2014.igem.org/Team:Berlin/Team">Team</a></li> | <li><a href="https://2014.igem.org/Team:Berlin/Team">Team</a></li> | ||
<li><a href="https://2014.igem.org/Team:Berlin/Safety">Safety</a></li> | <li><a href="https://2014.igem.org/Team:Berlin/Safety">Safety</a></li> | ||
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<li><a href="https://2014.igem.org/Team:Berlin/Workshop">Workshop</a></li> | <li><a href="https://2014.igem.org/Team:Berlin/Workshop">Workshop</a></li> | ||
<li><a href="https://2014.igem.org/Team:Berlin/Blog">Blog</a></li> | <li><a href="https://2014.igem.org/Team:Berlin/Blog">Blog</a></li> | ||
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<div class="row featurette" id="main-featurette-row"> | <div class="row featurette" id="main-featurette-row"> | ||
| - | <div class="start-text-container"> | + | <div class="start-text-container-project"> |
| - | <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" /> | + | <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Explore our Project:</h2> |
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<!--<a href="#" class="more-informations-white">More informations</a>--> | <!--<a href="#" class="more-informations-white">More informations</a>--> | ||
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<!--- ////////////////// DESCRIPTION////////////////// --> | <!--- ////////////////// DESCRIPTION////////////////// --> | ||
<div class="container"> | <div class="container"> | ||
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| - | + | <div class="col-xs-3 submenue-project" style="text-align:left;"> | |
| - | + | <a href="https://2014.igem.org/Team:Berlin/Project" class="sub-link-project"> 1. What is it all about?</a><br/><br/> | |
| - | + | <a href="https://2014.igem.org/Team:Berlin/Project/Activities" class="sub-link-project"> 2. Project-related Activities</a><br/><br/> | |
| - | + | <a href="https://2014.igem.org/Team:Berlin/Project/Detailed-Description" class="sub-link-project">3. Detailed Description</a><br/><br/> | |
| - | + | <a href="https://2014.igem.org/Team:Berlin/Project/Results" class="sub-link-project">4. Our Results</a><br/><br/> | |
| - | + | <a href="https://2014.igem.org/Team:Berlin/Project/Summary" class="sub-link-project">5. Lab Summary</a><br/><br/> | |
| - | + | <a href="https://2014.igem.org/Team:Berlin/Project/Journal" class="sub-link-project">6. Lab Journal</a><br/><br/> | |
| - | + | <a href="https://2014.igem.org/Team:Berlin/Project/Property" class="sub-link-project">7. Intellectual Property</a><br/><br/> | |
| + | <br/> | ||
| + | </div> | ||
| - | + | <div class="col-xs-11 col-sm-9 blog-text" style="margin-bottom:40px;"> | |
| - | <div class="col-xs- | + | |
<div class="project-number">1</div><div class="project-headline-float"><h2 class="green-text project-headline"> What is it all about?</h2></div> | <div class="project-number">1</div><div class="project-headline-float"><h2 class="green-text project-headline"> What is it all about?</h2></div> | ||
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<p> | <p> | ||
<a name="description"> </a> | <a name="description"> </a> | ||
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| - | + | <strong>iGEM Berlin 2014: A remote control for E. coli</strong><br/> | |
| - | + | <p> | |
| - | + | As the first iGEM team from Berlin, we decided to construct a simple BioBrick that enables synthetic biologists to remotely control the movement of E. coli. A seemingly simple and non-invasive mechanism for this remote control is the use of magnetic fields. By altering the iron homeostasis of E. coli, we want to increase the total iron level of the cytosol. By sequestering iron in a ferritin protein, iron crystals are formed and the cell is detoxified. We also worked with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to create various metal nanoparticles as an alternative strategy. Furthermore, we searched for the optimal conditions which yielded the most efficient formation of magnetic nanoparticles in E. coli. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used to remote control the cellular movement of E. coli.</p><br/> | |
| - | + | The following animation visualizes the concept of using ferritin iron storage proteins as a magnetism mediating module.<br/> | |
| - | + | <br/> | |
| - | + | <iframe src="//player.vimeo.com/video/109249906?byline=0&portrait=0&color=0ecd28" width="700" height="394" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen></iframe> | |
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| - | + | This animation was developed by Florian Renner who is an science interested and very talented graphic designer based in Munich. We thank Florian for his amazing job he did voluntarily for iGEM Berlin helping us to overcome the limitations of our budget. | |
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<img src="https://static.igem.org/mediawiki/2014/7/79/Team_Berlin_tuberlin_logo.png" class="img-responsive grayscale"> | <img src="https://static.igem.org/mediawiki/2014/7/79/Team_Berlin_tuberlin_logo.png" class="img-responsive grayscale"> | ||
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| + | <div class="col-sm-2 col-sm-offset-1 col-xs-3"> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/c/cb/Team_Berlin_florian_renner_logo.png" class="img-responsive grayscale"> | ||
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<a href="http://igem.berlin/team.php"><li>Team |</li></a> | <a href="http://igem.berlin/team.php"><li>Team |</li></a> | ||
<a href="http://igem.berlin/safety.php">Safety |</li></a> | <a href="http://igem.berlin/safety.php">Safety |</li></a> | ||
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<a href="http://igem.berlin/workshop2014_neu.php"><li>Workshop |</li></a> | <a href="http://igem.berlin/workshop2014_neu.php"><li>Workshop |</li></a> | ||
<a href="http://igem.berlin/blog.php"><li>Blog</li></a> | <a href="http://igem.berlin/blog.php"><li>Blog</li></a> | ||
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Latest revision as of 21:59, 17 October 2014
Explore our Project:
Explore our Project:1
What is it all about?
iGEM Berlin 2014: A remote control for E. coli
As the first iGEM team from Berlin, we decided to construct a simple BioBrick that enables synthetic biologists to remotely control the movement of E. coli. A seemingly simple and non-invasive mechanism for this remote control is the use of magnetic fields. By altering the iron homeostasis of E. coli, we want to increase the total iron level of the cytosol. By sequestering iron in a ferritin protein, iron crystals are formed and the cell is detoxified. We also worked with other metal binding proteins such as metallothioneins and phytochelatin synthases in order to create various metal nanoparticles as an alternative strategy. Furthermore, we searched for the optimal conditions which yielded the most efficient formation of magnetic nanoparticles in E. coli. Once we have discovered the best way to magnetize E. coli bacteria, we will build and characterize suitable BioBricks that can be used to remote control the cellular movement of E. coli.
The following animation visualizes the concept of using ferritin iron storage proteins as a magnetism mediating module.
This animation was developed by Florian Renner who is an science interested and very talented graphic designer based in Munich. We thank Florian for his amazing job he did voluntarily for iGEM Berlin helping us to overcome the limitations of our budget.
Sponsors
