Team:GeorgiaTech/Parts
From 2014.igem.org
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<th width="20%">Name</th><th width="20%">Type</th><th width="50%">Description</th><th width="10%">Length</th> | <th width="20%">Name</th><th width="20%">Type</th><th width="50%">Description</th><th width="10%">Length</th> | ||
- | <tr><td>BBa_K1539001<td>Part<td>Coding sequence has a high efficiency promoter followed by a high efficiency RBS to express fluorescent protein mCherry.<td> | + | <tr><td>BBa_K1539001<td>Part<td>Coding sequence has a high efficiency promoter followed by a high efficiency RBS to express fluorescent protein mCherry.<td>814 bp |
- | <tr><td>BBa_K1539002<td>Part<td>Coding sequence has a low efficiency promoter followed by a low efficiency RBS to express fluorescent protein mCherry.<td> | + | <tr><td>BBa_K1539002<td>Part<td>Coding sequence has a low efficiency promoter followed by a low efficiency RBS to express fluorescent protein mCherry.<td>814 bp |
+ | <tr><td>BBa_K1539003<td>Part<td>This coding sequence is for the beta subunit for Protein A (composed of three subunits) of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli.<td>1170 bp | ||
+ | <tr><td>BBa_K1539005<td>Part<td>This coding sequence is for the gamma subunit for Protein A (composed of three subunits) of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli.<td>513 bp | ||
+ | <tr><td>BBa_K1539008<td>Part<td>This coding sequence is for the gene sMMO B, encoding Protein B of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli.<td>426 bp | ||
+ | <tr><td>BBa_K1539013<td>Part<td>This coding sequence is for the gene sMMO C, encoding Protein C of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli.<td>1047 bp | ||
+ | <tr><td>BBa_K1539021<td>Device<td>This primer is used to insert a high efficiency RBS to transcribe genes starting with ATG in E. coli.<td>46 bp | ||
+ | <tr><td>BBa_K1539034<td>Device<td>This primer is used to insert a low efficiency RBS to transcribe genes starting with ATG in E. coli.<td>46 bp | ||
+ | <tr><td>BBa_K1539055<td>Device<td>This primer is used to insert a high efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design.<td>58 bp | ||
+ | <tr><td>BBa_K1539089<td>Device<td>This primer is used to insert a low efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design.<td>58 bp | ||
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Latest revision as of 05:26, 15 October 2014
Biobricks and Devices
Name | Type | Description | Length |
---|---|---|---|
BBa_K1539001 | Part | Coding sequence has a high efficiency promoter followed by a high efficiency RBS to express fluorescent protein mCherry. | 814 bp |
BBa_K1539002 | Part | Coding sequence has a low efficiency promoter followed by a low efficiency RBS to express fluorescent protein mCherry. | 814 bp |
BBa_K1539003 | Part | This coding sequence is for the beta subunit for Protein A (composed of three subunits) of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli. | 1170 bp |
BBa_K1539005 | Part | This coding sequence is for the gamma subunit for Protein A (composed of three subunits) of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli. | 513 bp |
BBa_K1539008 | Part | This coding sequence is for the gene sMMO B, encoding Protein B of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli. | 426 bp |
BBa_K1539013 | Part | This coding sequence is for the gene sMMO C, encoding Protein C of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli. | 1047 bp |
BBa_K1539021 | Device | This primer is used to insert a high efficiency RBS to transcribe genes starting with ATG in E. coli. | 46 bp |
BBa_K1539034 | Device | This primer is used to insert a low efficiency RBS to transcribe genes starting with ATG in E. coli. | 46 bp |
BBa_K1539055 | Device | This primer is used to insert a high efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design. | 58 bp |
BBa_K1539089 | Device | This primer is used to insert a low efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design. | 58 bp |