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Team:EPF Lausanne/Parts
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<div id="parts"> | <div id="parts"> | ||
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| - | + | ||
<h1 class="cntr">PARTS</h1> | <h1 class="cntr">PARTS</h1> | ||
| + | <br/> | ||
| - | + | <section id="dna"> | |
<h3 class="section-heading">DNA parts submitted by the 2014 EPFL iGEM team</h3> | <h3 class="section-heading">DNA parts submitted by the 2014 EPFL iGEM team</h3> | ||
<p class="lead"> | <p class="lead"> | ||
| - | Our team submitted a total of | + | Our team submitted a total of 55 Biobricks (part 51 does not exist).</p> |
| - | + | <p class="lead"> | |
| + | In addition, 4 microfluidic designs have also been submitted to the registry.</p> | ||
<table class="table table-striped table-hover" id="biobricks_list"> | <table class="table table-striped table-hover" id="biobricks_list"> | ||
<tr> | <tr> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486001</td> | <td class="biobrick_name">BBa_K1486001</td> | ||
| - | <td> | + | <td>Arabinose promoter + CpxR</td> |
| - | <td> | + | <td>Transcription factor</td> |
<td> </td> | <td> </td> | ||
<td>Bacteria</td> | <td>Bacteria</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486002</td> | <td class="biobrick_name">BBa_K1486002</td> | ||
| - | <td> | + | <td> Arabinose promoter + sfGFP-CpxR [Nterm]</td> |
<td>Expresses fused protein</td> | <td>Expresses fused protein</td> | ||
<td>Test CpxR expression & Ara promoter</td> | <td>Test CpxR expression & Ara promoter</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486003</td> | <td class="biobrick_name">BBa_K1486003</td> | ||
| - | <td>Flexible linker</td> | + | <td>Flexible linker 2x (GGGS)</td> |
<td>Attaches two proteins together</td> | <td>Attaches two proteins together</td> | ||
<td> </td> | <td> </td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486004</td> | <td class="biobrick_name">BBa_K1486004</td> | ||
| - | <td>Flexible linker</td> | + | <td>Flexible linker 2x (GGGGS)</td> |
<td>Attaches two proteins together</td> | <td>Attaches two proteins together</td> | ||
<td> </td> | <td> </td> | ||
| Line 140: | Line 185: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486005</td> | <td class="biobrick_name">BBa_K1486005</td> | ||
| - | <td> | + | <td>Arabinose promoter + sfGFP-CpxR [Cterm]</td> |
<td>Expresses fused protein</td> | <td>Expresses fused protein</td> | ||
<td>Test CpxR expression & Ara promoter</td> | <td>Test CpxR expression & Ara promoter</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486008</td> | <td class="biobrick_name">BBa_K1486008</td> | ||
| - | <td> | + | <td>CpxR & Split IFP1.4 [Cterm + Cterm]</td> |
<td>Two CpxR CDS, each C terminus attached to a moiety of IFP</td> | <td>Two CpxR CDS, each C terminus attached to a moiety of IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486009</td> | <td class="biobrick_name">BBa_K1486009</td> | ||
| - | <td> | + | <td>CpxR & Split IFP1.4 [Nterm + Nterm]</td> |
<td>Two CpxR CDS, each N terminus attached to a moiety of IFP</td> | <td>Two CpxR CDS, each N terminus attached to a moiety of IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
| Line 175: | Line 220: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486010</td> | <td class="biobrick_name">BBa_K1486010</td> | ||
| - | <td> | + | <td>CpxR & Split IFP1.4 [Nterm + Cterm]</td> |
<td>Two CpxR CDS, each attached to a moiety of IFP</td> | <td>Two CpxR CDS, each attached to a moiety of IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486011</td> | <td class="biobrick_name">BBa_K1486011</td> | ||
| - | <td> | + | <td>CpxR & Split IFP1.4 [Cterm + Nterm]</td> |
<td>Two CpxR CDS, each attached to a moiety of IFP</td> | <td>Two CpxR CDS, each attached to a moiety of IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486012</td> | <td class="biobrick_name">BBa_K1486012</td> | ||
| - | <td>CpxR | + | <td>CpxR-IFP[1] under pBAD</td> |
<td>CpxR with the Nterm moiety of IFP attached at its C terminus</td> | <td>CpxR with the Nterm moiety of IFP attached at its C terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486013</td> | <td class="biobrick_name">BBa_K1486013</td> | ||
| - | <td>CpxR | + | <td>CpxR-IFP[2] under pBAD</td> |
<td>CpxR with the Cterm moiety of IFP attached at its C terminus</td> | <td>CpxR with the Cterm moiety of IFP attached at its C terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486014</td> | <td class="biobrick_name">BBa_K1486014</td> | ||
| - | <td>IFP[1] | + | <td>IFP[1]-CpxR under pBAD</td> |
<td>CpxR with the Nterm moiety of IFP attached at its N terminus</td> | <td>CpxR with the Nterm moiety of IFP attached at its N terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486015</td> | <td class="biobrick_name">BBa_K1486015</td> | ||
| - | <td>IFP[2] | + | <td>IFP[2]-CpxR under pBAD</td> |
<td>CpxR with the Cterm moiety of IFP attached at its N terminus</td> | <td>CpxR with the Cterm moiety of IFP attached at its N terminus</td> | ||
<td>Intermediate & control</td> | <td>Intermediate & control</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486018</td> | <td class="biobrick_name">BBa_K1486018</td> | ||
| - | <td> | + | <td>Arabinose promoter + fLuc[1] + fLuc[2]</td> |
<td>Split firefly luciferase under arabinose promoter</td> | <td>Split firefly luciferase under arabinose promoter</td> | ||
<td>Control</td> | <td>Control</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486021</td> | <td class="biobrick_name">BBa_K1486021</td> | ||
| - | <td> | + | <td>Arabinose promoter + rLuc[1] + rLuc[2]</td> |
<td>Split renilla luciferase under arabinose promoter</td> | <td>Split renilla luciferase under arabinose promoter</td> | ||
<td>Control</td> | <td>Control</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486022</td> | <td class="biobrick_name">BBa_K1486022</td> | ||
| - | <td> | + | <td> Renilla Luciferase</td> |
<td>Full renilla luciferase</td> | <td>Full renilla luciferase</td> | ||
<td>Control</td> | <td>Control</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486023</td> | <td class="biobrick_name">BBa_K1486023</td> | ||
| - | <td>Yeast | + | <td>Yeast optimized superfolder GFP</td> |
| - | <td> | + | <td>Yeast optimised superfolder GFP</td> |
<td>Reporter</td> | <td>Reporter</td> | ||
<td>Yeast</td> | <td>Yeast</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486024</td> | <td class="biobrick_name">BBa_K1486024</td> | ||
| - | <td> | + | <td>Yeast kanamycin resistance</td> |
| - | <td>Yeast kanamycin resistance | + | <td>Yeast kanamycin resistance</td> |
<td>Selection marker</td> | <td>Selection marker</td> | ||
<td>Yeast</td> | <td>Yeast</td> | ||
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<td>ADH1 terminator</td> | <td>ADH1 terminator</td> | ||
<td>Terminator</td> | <td>Terminator</td> | ||
| - | <td> </td> | + | <td>Abortion of the transcription at the end of our DNA sequences</td> |
<td>Yeast</td> | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486026</td> | <td class="biobrick_name">BBa_K1486026</td> | ||
| - | <td> | + | <td>sfGFP + Kanamycin resistance for yeast </td> |
| - | <td> | + | <td>Tag</td> |
| - | <td>Control the expression of | + | <td>Control for the expression of Pbs2</td> |
<td>Yeast</td> | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486027</td> | <td class="biobrick_name">BBa_K1486027</td> | ||
| - | <td> | + | <td>R.reniformis luciferase + ADH1 terminator + Kanamycin resistance</td> |
| - | <td> | + | <td>Tag</td> |
| - | <td> </td> | + | <td>Control for the expression of Pbs2 </td> |
<td>Yeast</td> | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486028</td> | <td class="biobrick_name">BBa_K1486028</td> | ||
| - | <td>Yeast sfGFP | + | <td>Yeast optimized sfGFP N-terminus (1-214)</td> |
| - | <td> | + | <td>Yeast optimized sfGFP N-terminus (1-214)</td> |
<td> </td> | <td> </td> | ||
<td>Yeast</td> | <td>Yeast</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486029</td> | <td class="biobrick_name">BBa_K1486029</td> | ||
| - | <td>sfGFP | + | <td>Yeast Optimized sfGFP-N + ADH1 terminator + Kanamycin resistance</td> |
| - | <td> | + | <td>N terminal moiety of split sfGFP</td> |
| - | <td> </td> | + | <td>Complementation Assay </td> |
<td>Yeast</td> | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486030</td> | <td class="biobrick_name">BBa_K1486030</td> | ||
| - | <td> | + | <td>rLucN + ADH1 terminator + Kanamycin resistance</td> |
| - | <td> | + | <td>N terminal moiety of split rLuc</td> |
| - | <td> </td> | + | <td>Complementation Assay </td> |
<td>Yeast</td> | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486031</td> | <td class="biobrick_name">BBa_K1486031</td> | ||
| - | <td> | + | <td>CaUra3 selection marker</td> |
| - | <td> | + | <td>selection marker</td> |
<td>Confer resistance to Uracil-deprived medium</td> | <td>Confer resistance to Uracil-deprived medium</td> | ||
<td>Yeast</td> | <td>Yeast</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486032</td> | <td class="biobrick_name">BBa_K1486032</td> | ||
| - | <td> | + | <td>sfGFP + ADH1 terminator + Ura3 cassette</td> |
| - | <td> | + | <td>Tag</td> |
| - | <td>Control the expression of hog1</td> | + | <td>Control for the expression of hog1</td> |
<td>Yeast</td> | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486033</td> | <td class="biobrick_name">BBa_K1486033</td> | ||
| - | <td> | + | <td>sfGFP + ADH1 terminator + Ura3 cassette</td> |
| - | <td> | + | <td>Tag</td> |
| - | <td>Control the expression of hog1</td> | + | <td>Control for the expression of hog1</td> |
<td>Yeast</td> | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486034</td> | <td class="biobrick_name">BBa_K1486034</td> | ||
| - | <td> | + | <td>Yeast optimized superfolder GFP C-terminus (215-238)</td> |
| - | <td>C | + | <td>Yeast optimized superfolder GFP C-terminus (215-238)</td> |
<td> </td> | <td> </td> | ||
<td>Yeast</td> | <td>Yeast</td> | ||
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<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486035</td> | <td class="biobrick_name">BBa_K1486035</td> | ||
| - | <td> | + | <td>Yeast Optimized sfGFP-C + ADH1 terminator + CaUra3 Cassette</td> |
| - | <td> | + | <td>C terminal moiety of split sfGFP</td> |
| - | <td> </td> | + | <td>Complementation Assay </td> |
<td>Yeast</td> | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486036</td> | <td class="biobrick_name">BBa_K1486036</td> | ||
| - | <td> | + | <td>rLucC + ADH1 terminator + CaUra3 cassette</td> |
| - | <td> | + | <td>C terminal moiety of split rLuc</td> |
| - | <td> </td> | + | <td>Complementation Assay </td> |
<td>Yeast</td> | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486037</td> | <td class="biobrick_name">BBa_K1486037</td> | ||
| + | <td>13 amino acids linker [GGGS GGGGS GGGS]</td> | ||
<td>linker</td> | <td>linker</td> | ||
| - | <td> | + | <td>Attach Pbs2 or Hog1 to our different tags and splits </td> |
| - | + | ||
<td>Yeast</td> | <td>Yeast</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486038</td> | <td class="biobrick_name">BBa_K1486038</td> | ||
| - | <td> | + | <td>sfGFPN</td> |
<td>N terminus moiety of split superfolder GFP</td> | <td>N terminus moiety of split superfolder GFP</td> | ||
<td> </td> | <td> </td> | ||
| Line 378: | Line 423: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486039</td> | <td class="biobrick_name">BBa_K1486039</td> | ||
| - | <td> | + | <td>sfGFPC</td> |
<td>C terminus moiety of split superfolder GFP</td> | <td>C terminus moiety of split superfolder GFP</td> | ||
<td> </td> | <td> </td> | ||
| Line 385: | Line 430: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486040</td> | <td class="biobrick_name">BBa_K1486040</td> | ||
| - | <td> | + | <td>sfGFPN + CpxR</td> |
<td>N terminus moiety of split sfGFP attached to CpxR</td> | <td>N terminus moiety of split sfGFP attached to CpxR</td> | ||
<td> </td> | <td> </td> | ||
| Line 392: | Line 437: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486041</td> | <td class="biobrick_name">BBa_K1486041</td> | ||
| - | <td> | + | <td>sfGFPC + CpxR</td> |
<td>C terminus moiety of split sfGFP attached to CpxR</td> | <td>C terminus moiety of split sfGFP attached to CpxR</td> | ||
<td> </td> | <td> </td> | ||
| Line 399: | Line 444: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486042</td> | <td class="biobrick_name">BBa_K1486042</td> | ||
| - | <td> | + | <td>Leucine Zipper</td> |
<td>Monomer of leucine zipper TF</td> | <td>Monomer of leucine zipper TF</td> | ||
<td> </td> | <td> </td> | ||
| Line 406: | Line 451: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486043</td> | <td class="biobrick_name">BBa_K1486043</td> | ||
| - | <td> | + | <td>Leucine Zipper + split rLuc</td> |
<td>Two Leucine Zipper monomers, each attached to a different split rLuc moiety</td> | <td>Two Leucine Zipper monomers, each attached to a different split rLuc moiety</td> | ||
<td>Control for split rLuc assays</td> | <td>Control for split rLuc assays</td> | ||
| Line 413: | Line 458: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486044</td> | <td class="biobrick_name">BBa_K1486044</td> | ||
| - | <td> | + | <td>IFP[1] Mutated</td> |
<td>Biobrick-compatible IFP[1]</td> | <td>Biobrick-compatible IFP[1]</td> | ||
<td> </td> | <td> </td> | ||
| Line 420: | Line 465: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486045</td> | <td class="biobrick_name">BBa_K1486045</td> | ||
| - | <td> | + | <td>IFP[2] Mutated</td> |
<td>Biobrick-compatible IFP[2]</td> | <td>Biobrick-compatible IFP[2]</td> | ||
<td> </td> | <td> </td> | ||
| Line 458: | Line 503: | ||
<td>Reporter of CpxR</td> | <td>Reporter of CpxR</td> | ||
<td>Test the direction of the complete CpxR promoter</td> | <td>Test the direction of the complete CpxR promoter</td> | ||
| + | <td>Bacteria</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="biobrick_name">BBa_K1486052</td> | ||
| + | <td>Spacer</td> | ||
| + | <td>40 bases placed between constructs</td> | ||
| + | <td>Separate two constructs in the same plasmid</td> | ||
<td>Bacteria</td> | <td>Bacteria</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486053</td> | <td class="biobrick_name">BBa_K1486053</td> | ||
| - | <td> | + | <td>10 aa linker</td> |
<td>10 amino-acid linker</td> | <td>10 amino-acid linker</td> | ||
<td>Attach CheY/Z to split luciferases</td> | <td>Attach CheY/Z to split luciferases</td> | ||
| Line 469: | Line 521: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486054</td> | <td class="biobrick_name">BBa_K1486054</td> | ||
| - | <td>CheY/CheZ rLuc</td> | + | <td>CheY/CheZ + split rLuc</td> |
<td>CheY and CheZ, each attached to a moiety of split renilla luciferase</td> | <td>CheY and CheZ, each attached to a moiety of split renilla luciferase</td> | ||
<td>Positive control for the split rLuc</td> | <td>Positive control for the split rLuc</td> | ||
| Line 476: | Line 528: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486055</td> | <td class="biobrick_name">BBa_K1486055</td> | ||
| - | <td>CheY/CheZ fLuc</td> | + | <td>CheY/CheZ + split fLuc</td> |
<td>CheY and CheZ, each attached to a moiety of split firefly luciferase</td> | <td>CheY and CheZ, each attached to a moiety of split firefly luciferase</td> | ||
<td>Positive control for the split fLuc</td> | <td>Positive control for the split fLuc</td> | ||
| Line 483: | Line 535: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486056</td> | <td class="biobrick_name">BBa_K1486056</td> | ||
| - | <td> | + | <td>CpxR & Split IFP1.4 [Cterm + Cterm][2]</td> |
<td>Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP</td> | <td>Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP</td> | ||
<td>Characterize CpxR dimerization</td> | <td>Characterize CpxR dimerization</td> | ||
| Line 490: | Line 542: | ||
</table> | </table> | ||
| + | </section> | ||
<br /><br /> | <br /><br /> | ||
| + | <section id="microfluidics"> | ||
<h3 class="section-heading">Microfluidics parts (chips created)</h3> | <h3 class="section-heading">Microfluidics parts (chips created)</h3> | ||
<p class="lead"> | <p class="lead"> | ||
| Line 499: | Line 553: | ||
<p class="lead">When designing the chips, the team took into account the future users and the current iGEM classification of parts. We considered it best to construct our chips as composite microfluidic parts, so their sub-parts could be used and combined in multiple ways. The flow and control layers can be separated and reused, as well as all the basic structures (chamber + connecting channel), nodes, array parts,...</p> | <p class="lead">When designing the chips, the team took into account the future users and the current iGEM classification of parts. We considered it best to construct our chips as composite microfluidic parts, so their sub-parts could be used and combined in multiple ways. The flow and control layers can be separated and reused, as well as all the basic structures (chamber + connecting channel), nodes, array parts,...</p> | ||
| - | + | <p>By clicking on each of the designs' name, you can download the original files.</p> | |
<!-- send all lines here: https://2014.igem.org/Team:EPF_Lausanne/Microfluidics/Designing --> | <!-- send all lines here: https://2014.igem.org/Team:EPF_Lausanne/Microfluidics/Designing --> | ||
<table class="table table-striped table-hover" id="chips_list"> | <table class="table table-striped table-hover" id="chips_list"> | ||
| Line 507: | Line 561: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>SmashColi</td> | + | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/b/b7/SmashColi_iGEM_EPFL_2014.zip">SmashColi</a></td> |
| - | <td>To be able to separate the chip in 4 different compartments and apply 4 different | + | <td>To be able to separate the chip in 4 different compartments and apply 4 different pressures on each row of chambers.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>BioPad</td> | + | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/3/33/TheBioPad_iGEM_EPFL_2014.zip">The BioPad</a></td> |
| - | <td> | + | <td>A large and simple microfluidic chip containing 6400 chambers in which the cells are contained in. Each chamber acts as a pixel for the BioPad project.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/b/b3/CleanColi_iGEM_EPFL_2014.zip">CleanColi</a></td> |
| - | <td> | + | <td>As a result of our Safety page, we decided to create a chip that is able to seal the bacteria in the chip, preventing them to leave the chip.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>FilterColi</td> | + | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/7/79/FilterColi_iGEM_EPFL_2014.zip">FilterColi</a></td> |
| - | <td> | + | <td>To successfully immerse cells in a certain solution, this chip was designed to flow in the new medium in the chambers instead of doing it by diffusion.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| + | </section> | ||
<br /><br /> | <br /><br /> | ||
| - | |||
</div> | </div> | ||
</div> | </div> | ||
| + | </div> | ||
</div> | </div> | ||
| + | <div class="col col-md-3"> | ||
| + | <nav id="affix-nav" class="sidebar hidden-sm hidden-xs"> | ||
| + | <ul class="nav sidenav box" data-spy="affix" data-offset-top="200" data-offset-bottom="400"> | ||
| + | <li class="active"><a href="#dna">DNA Parts</a></li> | ||
| + | <li><a href="#microfluidics">Microfluidics Parts</a></li> | ||
| + | </ul> | ||
| + | </nav> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
<!-- END ABSTRACT --> | <!-- END ABSTRACT --> | ||
Latest revision as of 01:45, 18 October 2014
PARTS
DNA parts submitted by the 2014 EPFL iGEM team
Our team submitted a total of 55 Biobricks (part 51 does not exist).
In addition, 4 microfluidic designs have also been submitted to the registry.
| Biobrick | What it is | Function | Why do we use it? | Group |
|---|---|---|---|---|
| BBa_K1486000 | CpxR coding sequence | Transcription factor | To make most of our biobricks! | Bacteria |
| BBa_K1486001 | Arabinose promoter + CpxR | Transcription factor | Bacteria | |
| BBa_K1486002 | Arabinose promoter + sfGFP-CpxR [Nterm] | Expresses fused protein | Test CpxR expression & Ara promoter | Bacteria |
| BBa_K1486003 | Flexible linker 2x (GGGS) | Attaches two proteins together | Bacteria | |
| BBa_K1486004 | Flexible linker 2x (GGGGS) | Attaches two proteins together | Bacteria | |
| BBa_K1486005 | Arabinose promoter + sfGFP-CpxR [Cterm] | Expresses fused protein | Test CpxR expression & Ara promoter | Bacteria |
| BBa_K1486006 | IFP[1] | N terminus of split IFP | Bacteria | |
| BBa_K1486007 | IFP[2] | C terminus of split IFP | Bacteria | |
| BBa_K1486008 | CpxR & Split IFP1.4 [Cterm + Cterm] | Two CpxR CDS, each C terminus attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486009 | CpxR & Split IFP1.4 [Nterm + Nterm] | Two CpxR CDS, each N terminus attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486010 | CpxR & Split IFP1.4 [Nterm + Cterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486011 | CpxR & Split IFP1.4 [Cterm + Nterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization | Bacteria |
| BBa_K1486012 | CpxR-IFP[1] under pBAD | CpxR with the Nterm moiety of IFP attached at its C terminus | Intermediate & control | Bacteria |
| BBa_K1486013 | CpxR-IFP[2] under pBAD | CpxR with the Cterm moiety of IFP attached at its C terminus | Intermediate & control | Bacteria |
| BBa_K1486014 | IFP[1]-CpxR under pBAD | CpxR with the Nterm moiety of IFP attached at its N terminus | Intermediate & control | Bacteria |
| BBa_K1486015 | IFP[2]-CpxR under pBAD | CpxR with the Cterm moiety of IFP attached at its N terminus | Intermediate & control | Bacteria |
| BBa_K1486016 | fLuc[1] | N terminus moiety of the firefly luciferase | Bacteria | |
| BBa_K1486017 | fLuc[2] | C terminus moiety of the firefly luciferase | Bacteria | |
| BBa_K1486018 | Arabinose promoter + fLuc[1] + fLuc[2] | Split firefly luciferase under arabinose promoter | Control | Bacteria |
| BBa_K1486019 | rLuc[1] | C terminus moiety of the renilla luciferase | Bacteria | |
| BBa_K1486020 | rLuc[2] | N terminus moiety of the renilla luciferase | Bacteria | |
| BBa_K1486021 | Arabinose promoter + rLuc[1] + rLuc[2] | Split renilla luciferase under arabinose promoter | Control | Bacteria |
| BBa_K1486022 | Renilla Luciferase | Full renilla luciferase | Control | Bacteria |
| BBa_K1486023 | Yeast optimized superfolder GFP | Yeast optimised superfolder GFP | Reporter | Yeast |
| BBa_K1486024 | Yeast kanamycin resistance | Yeast kanamycin resistance | Selection marker | Yeast |
| BBa_K1486025 | ADH1 terminator | Terminator | Abortion of the transcription at the end of our DNA sequences | Yeast |
| BBa_K1486026 | sfGFP + Kanamycin resistance for yeast | Tag | Control for the expression of Pbs2 | Yeast |
| BBa_K1486027 | R.reniformis luciferase + ADH1 terminator + Kanamycin resistance | Tag | Control for the expression of Pbs2 | Yeast |
| BBa_K1486028 | Yeast optimized sfGFP N-terminus (1-214) | Yeast optimized sfGFP N-terminus (1-214) | Yeast | |
| BBa_K1486029 | Yeast Optimized sfGFP-N + ADH1 terminator + Kanamycin resistance | N terminal moiety of split sfGFP | Complementation Assay | Yeast |
| BBa_K1486030 | rLucN + ADH1 terminator + Kanamycin resistance | N terminal moiety of split rLuc | Complementation Assay | Yeast |
| BBa_K1486031 | CaUra3 selection marker | selection marker | Confer resistance to Uracil-deprived medium | Yeast |
| BBa_K1486032 | sfGFP + ADH1 terminator + Ura3 cassette | Tag | Control for the expression of hog1 | Yeast |
| BBa_K1486033 | sfGFP + ADH1 terminator + Ura3 cassette | Tag | Control for the expression of hog1 | Yeast |
| BBa_K1486034 | Yeast optimized superfolder GFP C-terminus (215-238) | Yeast optimized superfolder GFP C-terminus (215-238) | Yeast | |
| BBa_K1486035 | Yeast Optimized sfGFP-C + ADH1 terminator + CaUra3 Cassette | C terminal moiety of split sfGFP | Complementation Assay | Yeast |
| BBa_K1486036 | rLucC + ADH1 terminator + CaUra3 cassette | C terminal moiety of split rLuc | Complementation Assay | Yeast |
| BBa_K1486037 | 13 amino acids linker [GGGS GGGGS GGGS] | linker | Attach Pbs2 or Hog1 to our different tags and splits | Yeast |
| BBa_K1486038 | sfGFPN | N terminus moiety of split superfolder GFP | Bacteria | |
| BBa_K1486039 | sfGFPC | C terminus moiety of split superfolder GFP | Bacteria | |
| BBa_K1486040 | sfGFPN + CpxR | N terminus moiety of split sfGFP attached to CpxR | Bacteria | |
| BBa_K1486041 | sfGFPC + CpxR | C terminus moiety of split sfGFP attached to CpxR | Bacteria | |
| BBa_K1486042 | Leucine Zipper | Monomer of leucine zipper TF | Bacteria | |
| BBa_K1486043 | Leucine Zipper + split rLuc | Two Leucine Zipper monomers, each attached to a different split rLuc moiety | Control for split rLuc assays | Bacteria |
| BBa_K1486044 | IFP[1] Mutated | Biobrick-compatible IFP[1] | Bacteria | |
| BBa_K1486045 | IFP[2] Mutated | Biobrick-compatible IFP[2] | Bacteria | |
| BBa_K1486046 | CpxR promoter FW | CpxR binding-region in forward direction | Bacteria | |
| BBa_K1486047 | CpxR promoter RV | CpxR binding-region in reverse direction | Bacteria | |
| BBa_K1486048 | CpxR reporter | Calgary's CpxR reporter repaired (sequence was missing) | To see when CpxR is active | Bacteria |
| BBa_K1486049 | CpxR promoter FW + RFP | Reporter of CpxR | Test the direction of the complete CpxR promoter | Bacteria |
| BBa_K1486050 | CpxR promoter RV + RFP | Reporter of CpxR | Test the direction of the complete CpxR promoter | Bacteria |
| BBa_K1486052 | Spacer | 40 bases placed between constructs | Separate two constructs in the same plasmid | Bacteria |
| BBa_K1486053 | 10 aa linker | 10 amino-acid linker | Attach CheY/Z to split luciferases | Bacteria |
| BBa_K1486054 | CheY/CheZ + split rLuc | CheY and CheZ, each attached to a moiety of split renilla luciferase | Positive control for the split rLuc | Bacteria |
| BBa_K1486055 | CheY/CheZ + split fLuc | CheY and CheZ, each attached to a moiety of split firefly luciferase | Positive control for the split fLuc | Bacteria |
| BBa_K1486056 | CpxR & Split IFP1.4 [Cterm + Cterm][2] | Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP | Characterize CpxR dimerization | Bacteria |
Microfluidics parts (chips created)
Our team designed and made 4 microfluidic chips. At the beginning, we also used the MITOMI chip.
When designing the chips, the team took into account the future users and the current iGEM classification of parts. We considered it best to construct our chips as composite microfluidic parts, so their sub-parts could be used and combined in multiple ways. The flow and control layers can be separated and reused, as well as all the basic structures (chamber + connecting channel), nodes, array parts,...
By clicking on each of the designs' name, you can download the original files.
| Name | Main Function |
|---|---|
| SmashColi | To be able to separate the chip in 4 different compartments and apply 4 different pressures on each row of chambers. |
| The BioPad | A large and simple microfluidic chip containing 6400 chambers in which the cells are contained in. Each chamber acts as a pixel for the BioPad project. |
| CleanColi | As a result of our Safety page, we decided to create a chip that is able to seal the bacteria in the chip, preventing them to leave the chip. |
| FilterColi | To successfully immerse cells in a certain solution, this chip was designed to flow in the new medium in the chambers instead of doing it by diffusion. |
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