Team:GeorgiaTech

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    <LI class="column" id="HOME"><a href="https://2014.igem.org/Team:GeorgiaTech"style="color:#FFE740"> Home</a></LI>
 
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    <LI class="column" id="PROJECT"><a href="https://2014.igem.org/Team:GeorgiaTech/Project"style="color:#FFE740"> Project</a>
 
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        <LI><A href="https://2014.igem.org/Team:GeorgiaTech/Parts"style="color:#FFE740">Biobricks</A></LI>       
 
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    <LI><A href="https://2014.igem.org/Team:GeorgiaTech/Modeling"style="color:#FFE740">Modeling</A></LI></UL></LI></LI>   
 
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    <LI class="column" id="SAFETY"><a href="https://2014.igem.org/Team:GeorgiaTech/Safety"style="color:#FFE740"> Safety</a></LI>
 
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    <LI class="column" id="NOTEBOOK" ><a href="https://2014.igem.org/Team:GeorgiaTech/Notebook"style="color:#FFE740"> Notebook</a>   
 
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    <LI><A href="https://2014.igem.org/Team:GeorgiaTech/Notebook/Protocols"style="color:#FFE740">Protocols</A></LI>       
 
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    <LI class="column" id="TEAM"><a href="https://2014.igem.org/Team:GeorgiaTech/Team"style="color:#FFE740"> Team </a>
 
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            <LI><A href="https://2014.igem.org/Team:GeorgiaTech/Team/Bios"style="color:#FFE740">Member</A></LI>
 
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            <LI><A href="https://igem.org/Team.cgi?year=2014&team_name=GeorgiaTech"style="color:#FFE740">Official Team Profile</A></LI></UL></LI>
 
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     <h2 class="onBlack">Doesn't rubber come from trees?</h2>
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     <h2 class="onBlack">How does dissolved methane from fracking endanger our water?</h2>
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Let your eyes (and mouse) wander to these trees to discover our ideas on how to help the environment and change the future of rubber production. Take a look at our short <strong>project description</strong> below.
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Look at this running stream and imagine how it would appear when contaminated with methane gas. If you pictured it as a flowing stream of seltzer water, then you're not far off.
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The growing demand for natural rubber causes deforestation of the rainforest and occupation of arable lands, due to the establishment of new plantations. If producing rubber by bacteria succeeds, production of natural rubber will not be limited to the regions where the rubber tree can grow. Rather, rubber can be produced even in barren lands.  
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The demand for natural gas to fuel growing industries has led to the development and increased use of fracking techniques to extract methane and other natural gases from shale veins deep in the Earth's crust. In the extraction process, up to several million gallons of fracking fluid are pumped into the well, all of which must be later treated for methane and other hydrocarbon contaminants.  
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Our project aims to enable a common bacteria to produce natural rubber while grown under controlled conditions.
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Our project aims to enable E. coli to produce soluble methane monooxygenase (sMMO) a protein capable of converting methane into less volatile methanol, which can then be treated by further remediation pathways or extracted for commercial use.
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Natural rubber is composed of molecules consisting of the substance IPP linked together like a chain. The common bacteria that we use (E. coli) already possesses the ability to produce the IPP, but it lacks the enzyme to connect the IPP links together into a chain.
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We introduced the enzyme that the rubber tree has for connecting the links into the bacteria. Furthermore, we introduced genes that allow the bacteria further production of the IPP links.  
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     <h2 class="onBlack">No, rubber is made in the lab.</h2>
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     <h2 class="onBlack">How can synthetic biology eliminate the methane threat?</h2>
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Just days before the deadline of iGEM 2013, we got the peaks that our entire summer built towards: Two small bumps on the NMR indicating the presence of bacterially produced rubber in our strain of E. coli. With a mad scramble to the finish line, our initial indication was reinforced. Click anywhere along this text to start the interactive tour, which will guide you along the path to rubber.
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Our lab is equipped with the tools to synthesize new genetic sequences and generate transgenic bacteria capable of taking methane out of the water, one bubble at a time.
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<h1 text-align="center">Our Generous Sponsors</h1>
<h1 text-align="center">Our Generous Sponsors</h1>
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         <LI class="column" id="Macrogen" style="left: 40px;"><p><a href="http://www.macrogenusa.com/"><img src="https://static.igem.org/mediawiki/2014/3/34/Macrogen.jpg" width="274px" height="100px"></a></p></LI>
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         <LI class="column" id="IDT" style="left: 40px;"><p><div><a href="http://www.idtdna.com/site"><img src="https://static.igem.org/mediawiki/2014/2/22/IDT-Logo.jpg" width="241px" height="100px"></a></LI>
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        <LI class="column" id="Qiagen" style="left: 40px;"><p><div><a href="http://www.qiagen.com/"><img src="https://static.igem.org/mediawiki/2014/9/91/Qiagen_logo.JPG" width="129px" height="100px"></a></LI>
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Latest revision as of 18:40, 17 October 2014

How does dissolved methane from fracking endanger our water?

Look at this running stream and imagine how it would appear when contaminated with methane gas. If you pictured it as a flowing stream of seltzer water, then you're not far off.

The demand for natural gas to fuel growing industries has led to the development and increased use of fracking techniques to extract methane and other natural gases from shale veins deep in the Earth's crust. In the extraction process, up to several million gallons of fracking fluid are pumped into the well, all of which must be later treated for methane and other hydrocarbon contaminants.

Our project aims to enable E. coli to produce soluble methane monooxygenase (sMMO) a protein capable of converting methane into less volatile methanol, which can then be treated by further remediation pathways or extracted for commercial use.

Our Generous Sponsors

Our Creative Commons Image Suppliers

  • Fire Background

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