Team:Hong Kong HKUST/wetlab/safety
From 2014.igem.org
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<div class= "content_area_one_row"> | <div class= "content_area_one_row"> | ||
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<h3>Biobrick Safety</h3> | <h3>Biobrick Safety</h3> | ||
<p> | <p> | ||
- | The team project will be using some non-virulent parts from a non-virulent strain of Streptococcus pneumoniae. | + | The team project will be using some non-virulent parts from a non-virulent strain of <i>Streptococcus pneumoniae</i>. |
- | To avoid any possible infection, we will not work on any form of live S. pneumoniae, but we will only work on | + | To avoid any possible infection, we will not work on any form of live <i>S. pneumoniae</i>, but we will only work on |
- | its genomic DNA where we requested from another laboratory.We are also using lab strains E. coli routinely used | + | its genomic DNA where we requested from another laboratory. We are also using lab strains <i>E. coli</i> routinely used |
in cloning like DH10B and DH5alpha which are safe to operate. | in cloning like DH10B and DH5alpha which are safe to operate. | ||
</p> | </p> | ||
<br> | <br> | ||
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<thead> | <thead> | ||
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<th>Risk Group Source</th> | <th>Risk Group Source</th> | ||
<th>Disease risk to humans?</th> | <th>Disease risk to humans?</th> | ||
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<th>How did you acquire it?</th> | <th>How did you acquire it?</th> | ||
<th>How will you use it?</th> | <th>How will you use it?</th> | ||
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</tr> | </tr> | ||
</thead> | </thead> | ||
- | <tbody> | + | <tbody style="text-align:center"> |
<tr> | <tr> | ||
<td> | <td> | ||
- | E.coli (DH10B) | + | <i>E.coli</i> (DH10B) |
</td> | </td> | ||
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<a href="http://tools.invitrogen.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf"> | <a href="http://tools.invitrogen.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf"> | ||
- | http://tools.invitrogen.com/content/sfs/msds/ | + | http://tools.invitrogen<br> |
+ | .com/content/sfs/msds/2<br> | ||
+ | 013/500015_MTR-NALT_EN.<br> | ||
+ | pdf</a> | ||
</td> | </td> | ||
<td> | <td> | ||
- | It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin | + | It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. |
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This organism is our chassis. | This organism is our chassis. | ||
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</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | E.coli (DH5alpha) | + | <i>E.coli</i> (DH5alpha) |
</td> | </td> | ||
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<a href="https://microbewiki.kenyon.edu/index.php/DH5-Alpha_E.coli"> | <a href="https://microbewiki.kenyon.edu/index.php/DH5-Alpha_E.coli"> | ||
- | https://microbewiki. | + | https://microbewiki.ken<br> |
+ | yon.edu/index.php/DH5-A<br> | ||
+ | lpha_E.coli</a> | ||
</td> | </td> | ||
<td> | <td> | ||
- | It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin | + | It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. |
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</td> | </td> | ||
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This organism is our chassis. | This organism is our chassis. | ||
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</td> | </td> | ||
</tr> | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <table id="safety_table" border="2px" align="center"> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Part number/name</th> | ||
+ | <th>Natural function of part</th> | ||
+ | <th>How did you acquire it?</th> | ||
+ | <th>How will you use it?</th> | ||
+ | <th>Notes</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody style="text-align:center"> | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | + | <i>comD</i> from <i>S. pneumoniae</i> | |
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- | This part produces a membrane-bound histidine-kinase receptor, ComD which enables competence-stimulating peptide (CSP) to bind. This serves as a detector of Streptococcus | + | This part produces a membrane-bound histidine-kinase receptor, ComD which enables competence-stimulating peptide (CSP) to bind. This serves as a detector of Streptococcus pneumoniae. |
</td> | </td> | ||
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- | + | <i>comE</i> from <i>S. pneumoniae</i> | |
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- | + | <i>comX</i> from <i>S. pneumoniae</i> </td> | |
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part DNA that encodes an alternative sigma factor that directs the transcription of late competence (com) genes. | part DNA that encodes an alternative sigma factor that directs the transcription of late competence (com) genes. | ||
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- | + | <i>comW</i> from <i>S. pneumoniae</i> | |
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collecting these chemical wastes and sending them to a centralized chemical waste disposal | collecting these chemical wastes and sending them to a centralized chemical waste disposal | ||
center within our university, where they will be processed by professionals in chemical waste treatment. | center within our university, where they will be processed by professionals in chemical waste treatment. | ||
- | The only living biological material that could escaped from the lab would be our E. coli host for DNA cloning and | + | The only living biological material that could escaped from the lab would be our <i>E. coli</i> host for DNA cloning and |
parts characterization, and there is little risk associated with their leakage because we are using DH10B as the | parts characterization, and there is little risk associated with their leakage because we are using DH10B as the | ||
major cloning host in which the strain is auxotrophic for leucine, and therefore rendering it unlikely to propagation | major cloning host in which the strain is auxotrophic for leucine, and therefore rendering it unlikely to propagation | ||
- | outside laboratory environment. The strains of E. coli we use in lab, DH10B and DH5alpha, are also routinely used lab | + | outside laboratory environment. The strains of <i>E. coli</i> we use in lab, DH10B and DH5alpha, are also routinely used lab |
strains that are innocuous and non-virulent. | strains that are innocuous and non-virulent. | ||
</p> | </p> | ||
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<h3>Researcher Safety</h3> | <h3>Researcher Safety</h3> | ||
<p> | <p> | ||
- | Tacking risk of organism, only non-virulent part was extracted from genomic DNA of non-virulent S. pneumoniae strain | + | Tacking risk of organism, only non-virulent part was extracted from genomic DNA of non-virulent <i>S. pneumoniae</i> strain |
obtained from Professor Patrick Chiu Yat Woo at Hong Kong University. | obtained from Professor Patrick Chiu Yat Woo at Hong Kong University. | ||
For chassis, only Biosafety Level 1 organisms (DH10B and DH5alpha) are dealt in the lab. | For chassis, only Biosafety Level 1 organisms (DH10B and DH5alpha) are dealt in the lab. | ||
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The Hong Kong University of Science and Technology, HKUST. | The Hong Kong University of Science and Technology, HKUST. | ||
We, team members, have not directly discussed our project with the director of HSEO but, our supervisors have. | We, team members, have not directly discussed our project with the director of HSEO but, our supervisors have. | ||
- | After discussing the risks, we decided to operate only on the genomic DNA of a non-virulent strain of | + | After discussing the risks, we decided to operate only on the genomic DNA of a non-virulent strain of <i>S. pneumoniae</i> and |
we are not handling the live bacteria itself. | we are not handling the live bacteria itself. | ||
<br> | <br> | ||
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<p> | <p> | ||
The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) | The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) | ||
- | can be directed to:< | + | can be directed to: |
- | <a href="http://www.ab.ust.hk/hseo/training.htm">http://www.ab.ust.hk/hseo/training.htm</a> | + | <br> |
+ | <a href="http://www.ab.ust.hk/hseo/training.htm">http://www.ab.ust.hk/hseo/training.htm</a></p> | ||
<br> | <br> | ||
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+ | <br> | ||
Chapter 9 Biological Safety. (2006, November 1). Retrieved October 3, 2014, from | Chapter 9 Biological Safety. (2006, November 1). Retrieved October 3, 2014, from | ||
<br> | <br> | ||
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+ | Life Technologies. (12 Mar 2013). Safety Data Sheet. Retrieved October 10, 2014, from | ||
+ | <br> | ||
+ | <a href="http://tools.lifetechnologies.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf"> | ||
+ | http://tools.lifetechnologies.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf | ||
+ | </a> | ||
+ | <br> | ||
+ | <br> | ||
+ | Microbe Wiki. (14 August 2013). DH5-Alpha <i>E. coli</i>. Retrieved October 10, 2014, from | ||
+ | <br> | ||
+ | <a href="https://microbewiki.kenyon.edu/index.php/DH5-Alpha_E.coli"> | ||
+ | https://microbewiki.kenyon.edu/index.php/DH5-Alpha_E.coli | ||
+ | </a> | ||
</p> | </p> | ||
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}} | }} |
Latest revision as of 01:37, 18 October 2014
Safety
Biobrick SafetyThe team project will be using some non-virulent parts from a non-virulent strain of Streptococcus pneumoniae. To avoid any possible infection, we will not work on any form of live S. pneumoniae, but we will only work on its genomic DNA where we requested from another laboratory. We are also using lab strains E. coli routinely used in cloning like DH10B and DH5alpha which are safe to operate.
Environmental and General Public SafetyImproper disposal of biological waste might result in leak of biological materials to the environment, which might cause contamination, and improper disposal might also cause infection to those who process the wastes. However, there is little biological risk associated with our project in the first place, as described above because we are using innocuous bacteria strains that are unlikely to propagate in the environment. Despite being so, we autoclave all solid biological wastes before they are discarded as landfill garbage. We also treat liquid waste with 10% bleach to ensure that no biological waste could escape to the environment. Commonly used chemicals are mostly buffers and therefore pose little threat and risk to the environment. For chemicals that are hazardous, they could cause damage to other people if leaked out. We avoid these risks by collecting these chemical wastes and sending them to a centralized chemical waste disposal center within our university, where they will be processed by professionals in chemical waste treatment. The only living biological material that could escaped from the lab would be our E. coli host for DNA cloning and parts characterization, and there is little risk associated with their leakage because we are using DH10B as the major cloning host in which the strain is auxotrophic for leucine, and therefore rendering it unlikely to propagation outside laboratory environment. The strains of E. coli we use in lab, DH10B and DH5alpha, are also routinely used lab strains that are innocuous and non-virulent. Researcher SafetyTacking risk of organism, only non-virulent part was extracted from genomic DNA of non-virulent S. pneumoniae strain obtained from Professor Patrick Chiu Yat Woo at Hong Kong University. For chassis, only Biosafety Level 1 organisms (DH10B and DH5alpha) are dealt in the lab. All members are trained to deliver safe lab practices. In dealing with toxic chemicals such as DMSO, we wear lab coats and gloves and execute under fume hood. All experiments are conducted in a lab premise under authorized supervision. Risks with handling toxic chemicals, including DMSO, phenol and chloroform, which include chemical burns and damage to the health of team members in the long run, have been addressed. We wear lab coats and gloves whenever we handle these chemicals to minimize exposure to these chemicals, and we carry out bulk management of these chemicals inside a well ventilated fume hood. Risks associated with UV exposure during gel documentation and excision are also minimized by wearing face shields and gloves until the UV light is off. University Safety
Health, Safety & Environment Office (HSEO) is responsible for safety regulations at our institution,
The Hong Kong University of Science and Technology, HKUST.
We, team members, have not directly discussed our project with the director of HSEO but, our supervisors have.
After discussing the risks, we decided to operate only on the genomic DNA of a non-virulent strain of S. pneumoniae and
we are not handling the live bacteria itself.
Safety Training Courses
This year's HKUST team members have received safety training over the summer. Topics that we have learned about
in safety training are as following:
The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST)
can be directed to:
Reference
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