Team:Heidelberg

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<div class="container">
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<a href="https://2014.igem.org" style="position:absolute;z-index:1;"><img src="/wiki/images/5/5d/IGEM_logo_white.png" style="height:70px;" alt="iGEM Logo" /></a>
<a href="https://2014.igem.org" style="position:absolute;z-index:1;"><img src="/wiki/images/5/5d/IGEM_logo_white.png" style="height:70px;" alt="iGEM Logo" /></a>
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<div class="col-lg-6 col-md-6 col-sm-12 impressiv">
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<h3>iGEM TEAM <span>HEIDELBERG</span> 2014</h3>
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<h3 class="normal-medium-text">iGEM TEAM <span>HEIDELBERG</span> 2014</h3>
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<h1>THE RING<br />OF <span>FIRE</span></h1>
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<h1 class="very-large-text">THE RING<br />OF <span>FIRE</span></h1>
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<p>Welcome to the proteins of tomorrow.<br/>This is the iGEM team Heidelberg‘s wiki page.</p>
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<p> Click <a href="/Team:Heidelberg/Project#Abstract">here</a> to view our abstract.
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<p style="font-size:30px"> Click <a href="/Team:Heidelberg/Project#Abstract">here</a> to view our abstract.
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Scroll down to explore our project.
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Scroll down to EXPLORE our project.
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<h1 style="text-align:right;"><span class="red-text" style="font-size:1.3em;">PLACEHOLDER</span></h1>
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<p><span class="larger red-text">Nature</span> has made many curious inventions. One of these are</p>
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<p><span class="red-text" style="font-size: 1.5em;">Nature</span> has made many curious inventions. One of these are circular proteins, which are conventional peptides that neither have a beginning, nor an ending</p>
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<h1 class="very-large-text red-text bold" style="text-align:right;"><a href="https://2014.igem.org/Team:Heidelberg/Toolbox/Circularization">CIRCULAR PROTEINS</a></h1>
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<p>Apart from other features, these proteins are extremely stable against
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<p>which are unconventional peptides that neither have a beginning, nor an ending</p><br>
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  high temperatures, pH and proteases.</p>
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<p class="normal-medium-text bold">These proteins are extremely <span class="red-text">resistant</span> against
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<p style="font-weight:bold;">We seeked to apply this principle of circularization in Synthetic Biology and create a way of rendering any protein heatstable.</p>
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  <span class="red-text">high temperatures</span>, pH changes and proteases.</p>
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<p class="normal-medium-text align-right">We established protein <a href="/Team:Heidelberg/Toolbox/Circularization"> circularization </a> as a new<br>powerful tool for Synthetic Biology and set the foundations<br>to render any protein heat stable.</p>
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<h2 style="text-align:center; font-weight: bold; margin-bottom:0;">Let us take you to the next level of bioengineering ...</h2>
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<h1 class="large-text bold">Wondering how we circularize?</h1>
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<h1 style="text-align: center; margin-top:0; font-size:3em; margin-bottom: 15px;"><span class="light-red-text">USING THE</span> Mechanism <span class="light-red-text">OF</span> SPLIT-INTEINS</h1>
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<h2 class="normal-medium-text align-right bold">Let us introduce you to the next generation of bioengineering...</h2>
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<h2 class="align-right" style="font-size: 40px; font-weight:normal">come DISCOVER the <a href="/Team:Heidelberg/Project/Background">MECHANISM of SPLIT INTEINS</a></h2><br><br>
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<p>Intein splicing is a natural process that excises one part of a protein and leaves the remaining parts irreersibly attached.</p>
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<p>When attaching split inteins to the ends of a normal protein, the splicing reaction connects the beginning to the ending and forms a circular protein.</p>
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<p class=""><span class="larger">Inteins excise themselves out of proteins and in doing so the remaining flanking parts are irreversibly joined</span></p>
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<p><span class="larger">–&nbsp;an effective mechanism to circularize proteins.</span></p>
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<a href="/Team:Heidelberg/Toolbox/Circularization">
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<p class="normal-medium-text">But messing with protein structure can be disastrous,<br><span class="medium-text">you can only win with good <a class="bold" href="/Team:Heidelberg/Modeling">MODELING</a>.</span></p><br><br>
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<img src="/wiki/images/5/58/Heidelberg_Toolbox_Circularization.png" id="circ-icon" class="toolbox-icon">&nbsp;CIRCULARIZATION
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<p class="normal-medium-text"><span>Find out the <span class="bold">EXCITING THEORY</span><br>behind <span class="bold">RIGID LINKERS</span> and their <span class="bold">ANGLES</span></span></p>
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<br>
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<p><span class="larger">We developed <a href="/Ream:Heidelberg/Software/Linker_Software">CRAUT</a>,<br>
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a comprehensive software which identifies<br>
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the optimal path to connect a protein’s termini<br>
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preserving structure and function.</span></p>
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<a href="/Team:Heidelberg/Toolbox/Oligomerization">
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<p><span class="larger">Look at our extensive wet-lab SCREENING to improve and calibrate our software using <a class="red-text" href="/Team:Heidelberg/Project/Linker_Screening">lambda lysozyme</a>!</span></p>
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<img src="/wiki/images/4/40/Oligomerization.png" id="oligo-icon" class="toolbox-icon">&nbsp;OLIGOMERIZATION
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<img src="/wiki/images/8/87/Heidelberg_Toolbox_Fusion.png" id="fusion-icon" class="toolbox-icon">&nbsp;FUSION
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<p class="align-right"><span class="larger">As calculations on protein structures are costly<br>we involved the rest of the world with</span></p>
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<h1 class="align-right bold very-large-text dark-red-text"><span style="font-size:1.5em;"><a href="/Team:Heidelberg/Human_Practice/igemathome">iGEM@home</a></span></h1>
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<h3 class="medium-text bold">Empowering science!</h3><br>
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<img src="/wiki/images/c/c2/Heidelberg_Toolbox_On-Off.png" id="onoff-icon" class="toolbox-icon">&nbsp;ON/OFF
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<p class="normal-medium-text"><a href="/Team:Heidelberg/Software/igemathome">NEW PLATFORM</a> for distributed computing.</p>
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<p><span class="larger">Volunteers provide the idle calacity of their home computers.</span></p>
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<p class=""><span class="larger">And, with an established user base with more than <span class="larger red-text">1000</span> computers, we effectively <a href="/Team:Heidelberg/Human_Practice/igemathome" >bring synthetic biology to society</a> in a new way!</span></p>
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<a href="/Team:Heidelberg/Human_Practice/igemathome" ><img style="position:relative; top: -35px;" class="img-responsive" src="/wiki/images/5/52/Heidelberg_Frontpage_igemathome_cloud.png"></a>
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<img src="/wiki/images/0/04/Heidelberg_Toolbox_Purification.png" id="purification-icon" class="toolbox-icon">&nbsp;PURIFICATION
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<h1 style="text-align: right; font-size: 3em;" >... and show you the  WORLD of <br />
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<span class="red-text">post-translational MODIFCATION</span>
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</h1>
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<p>The iGEM Team Heidelberg has developed an intein toolbox for the iGEM community to easily modify your protein in a standarized method.</p>
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<p>Our toolbox contains several tools which are supergeil. Here you can find out more about our Toolbox.</p>
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<p>In addition to that all tools are inducible by light. Using the LOV system we built a solid method for regulation of the intein trans-splicing reaction our toolbox consiting on. Click here to get more informations about Induction.</p>
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<a href="/Team:Heidelberg/Project/PCR_2.0" ><img class="img-responsive" src="/wiki/images/a/a6/Heidelberg_Project_Dnmt1.png"></a>
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<h1 class="dark-grey-text" style="text-align: right;"><span style="font-size: 0.8em;">circular <span class="red-text">heat-stable</span></span><br>DNMT1</h1>
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<h1 class="large-text">Our Application:</h1>
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<p>Wouldn´t it be great to amplify DNA in a normal PCR maintaining the epigenetic information coded in methylation patterns?</p>
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<h1 class="dark-grey-text large-text" style="text-align: right;"><span class="medium-large-text">circular <a href="/Team:Heidelberg/Project/PCR_2.0">heat-stable</a></span><br>DNA-methyltransferase</h1>
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<p>The problem: DNMT I, an enzyme which is responsible for the establishment and maintenance of the individual methylation pattern of different cell types, is not heat stable.
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<br><br><p><span class="larger">Wouldn´t it be great to amplify DNA preserving the encoded epigenetic information?</span></p>
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For iGEM 2014 we therefore create a PCR 2.0 with heat-stable DNMT I by circularization.
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<p><span class="larger">This could lead to an ENTIRELY NEW way of performing PCRs with a <a href="/Team:Heidelberg/Project/PCR_2.0">heat stable methyltransferase</a>!</span></p>
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<p>&nbsp;<br /><span class="larger">We'd like to call it</span></p>  
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<span class="title-dnmt1">PCR 2.0</span>
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<a href="/Team:Heidelberg/Project/PCR_2.0" class="title-dnmt1">PCR 2.0</a>
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<h1 style="text-align: right; color: black; padding-right: 130px;" ><span style="font-size: 0.8em;">circular <span style="color:white;">heat-stable</span></span><br><span style="font-size:1.3em;">Xylanase</span></h1>
 
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<p>Xylanase is an important enzyme for the pulp and paper industry.</p>
 
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<p>Bla bla</p>
 
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<p>In future Xylanase could be used for the production of biofuel.</p>
 
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<span class="title-xylanase">INDUSTRY</span>
 
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<img src="/wiki/images/5/58/Heidelberg_Toolbox_Circularization.png" id="circ-icon" class="toolbox-icon">&nbsp;&nbsp;CIRCULARIZATION
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<h1 style="text-align: left;" ><span style="font-size:1.3em;">LINK it!</span></h1>
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<a href="https://2014.igem.org/Team:Heidelberg/Project/Toolbox#Oligomerization">
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<p>Could every protein becomes heat stable by circularization, even if it´s the most complex of all?</p>
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<img src="/wiki/images/4/40/Oligomerization.png" id="oligo-icon" class="toolbox-icon">&nbsp;&nbsp;OLIGOMERIZATION
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<p>Circularization is a narrow path between gaining heat-stability and loosing function due to deformation. We developed a linker software, which predict the perfect linker depending on the folding structure of every protein.</p>
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<p>In an extensive linker screening our software was improved and calibrated using the lambda phage lysozyme.</p>
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<a href="https://2014.igem.org/Team:Heidelberg/Project/Toolbox#Fusion_and_Tagging">
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<img src="/wiki/images/8/87/Heidelberg_Toolbox_Fusion.png" id="fusion-icon" class="toolbox-icon">&nbsp;&nbsp;FUSION
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<a href="https://2014.igem.org/Team:Heidelberg/Project/Toolbox#Purification">
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<img src="/wiki/images/0/04/Heidelberg_Toolbox_Purification.png" id="purification-icon" class="toolbox-icon">&nbsp;&nbsp;PURIFICATION
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<h2 class="normal-medium-text align-right">One more thing.<br>Inteins are capable of much more!</h2>
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<h1 class="medium-large-text align-right bold">We show you the world of</h1>
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<h1 class="medium-text align-right red-text bold" style="font-size: 41px;">post-translational MODIFICATIONS</h1><br><br>
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<p class="align-right "><span class="larger">We created an <a href="/Team:Heidelberg/Project/Toolbox">INTEIN TOOLBOX</a> so you can easily modify your protein in a standardized way.</span></p>
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<p class="align-right"><span class="larger">Take a look at our <a href="/Team:Heidelberg/Parts/RFC">RFC</a>!</span></p>
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<p class="align-right"><span class="larger">Explore all our tools!</span></p>
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<h1>Who are we?</h1>
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<p>We are the iGEM Team Heidelberg 2014 consisting of 12 highly motivated bachelor and master students studying at Heidelberg University.</p>
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<h1 style="text-align: left; color:#DE4230;" ><span style="font-size:1.5em; font-weights:bold;">CALCULATE it!</span></h1>
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<p>For our project we got great feedback and support from our supervisors.</p>
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<p>Take a look at our <a href="/Team:Heidelberg/Team">Teampage</a>!</p>
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<h1>Thank you!</h1>
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<p>We want thank all people who helped us and supported our work in the lab.</p>
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<p><br/>After calculating eleven days and the breakdown of both computational and mental power we decided to spread the modeling of the linkers.</p>
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<p>The iGEM Team Heidelberg developed <a href="/Team:Heidelberg/Software/igemathome">iGEM@home</a>, a software to divide extensive computing task into many packages and to distribute them to many computers. Now over 1.000 volunteers are calculating for us when their are idle.</p>
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Latest revision as of 23:43, 16 October 2014

iGEM Logo
Ring of fire Image

iGEM TEAM HEIDELBERG 2014

THE RING
OF FIRE

Click here to view our abstract.
Scroll down to EXPLORE our project.

Nature has made many curious inventions. One of these are

CIRCULAR PROTEINS

which are unconventional peptides that neither have a beginning, nor an ending


These proteins are extremely resistant against high temperatures, pH changes and proteases.

We established protein circularization as a new
powerful tool for Synthetic Biology and set the foundations
to render any protein heat stable.

Wondering how we circularize?

Let us introduce you to the next generation of bioengineering...

come DISCOVER the MECHANISM of SPLIT INTEINS



Inteins excise themselves out of proteins and in doing so the remaining flanking parts are irreversibly joined

– an effective mechanism to circularize proteins.

But messing with protein structure can be disastrous,
you can only win with good MODELING.



Find out the EXCITING THEORY
behind RIGID LINKERS and their ANGLES


We developed CRAUT,
a comprehensive software which identifies
the optimal path to connect a protein’s termini
preserving structure and function.

Look at our extensive wet-lab SCREENING to improve and calibrate our software using lambda lysozyme!

As calculations on protein structures are costly
we involved the rest of the world with

iGEM@home

Empowering science!


NEW PLATFORM for distributed computing.

Volunteers provide the idle calacity of their home computers.

And, with an established user base with more than 1000 computers, we effectively bring synthetic biology to society in a new way!

Our Application:

circular heat-stable
DNA-methyltransferase



Wouldn´t it be great to amplify DNA preserving the encoded epigenetic information?

This could lead to an ENTIRELY NEW way of performing PCRs with a heat stable methyltransferase!

 
We'd like to call it

One more thing.
Inteins are capable of much more!

We show you the world of

post-translational MODIFICATIONS



We created an INTEIN TOOLBOX so you can easily modify your protein in a standardized way.

Take a look at our RFC!

Explore all our tools!

Who are we?

We are the iGEM Team Heidelberg 2014 consisting of 12 highly motivated bachelor and master students studying at Heidelberg University.

For our project we got great feedback and support from our supervisors.

Take a look at our Teampage!

Thank you!

We want thank all people who helped us and supported our work in the lab.

Retrieved from "http://2014.igem.org/Team:Heidelberg"