Team:Tongji/lab-archives-zh

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                  <li ><a  href="https://2014.igem.org/Team:Tongji/lab-archives"  >English</a></li>
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          <p class="lead">点击“下载”按钮，您可以查看我们的重要实验方案。</p>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/81/Tongji-Notebook.png" alt="notebook">
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            <div class="caption">
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              <h5>PCR方案</h5>
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              <p>XynB，ArfB和ManA1的PCR方案（英文）</p>
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              <p><a  onclick=window.parent.location.href="https://static.igem.org/mediawiki/2014/d/d2/IGEM_Tongji_Protocol_PCR.pdf" class="btn btn-primary">下载</a></p>
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            <div class="caption">
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              <h5>细菌培养方案</h5>
 +
              <p><em>Clostridium stercorarium</em>的培养方案（英文）</p>
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              <div class="nelson">
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              <p><a  onclick=window.parent.location.href="https://static.igem.org/mediawiki/2014/9/93/IGEM_Tongji_Protocol_CSCultivation.pdf" class="btn btn-primary">下载</a></p>
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            <h1>记录</h1>
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          <p class="lead">点击“下载”按钮，您可以查看我们的重要实验记录。</p>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/81/Tongji-Notebook.png" alt="notebook">
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            <div class="caption">
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              <h5>载体构建</h5>
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              <p>表达载体构建报告</p>
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              <div class="nelson">
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              <p><a  href="https://static.igem.org/mediawiki/2014/5/51/Report_for_expression_vector_construction2.pdf" class="btn btn-primary">下载</a></p>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/81/Tongji-Notebook.png" alt="notebook">
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            <div class="caption">
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              <h5>表达验证</h5>
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              <p>大肠杆菌蛋白表达报告</p>
 +
              <div class="nelson">
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              <p><a href="https://static.igem.org/mediawiki/2014/0/07/Report_for_protein_expression_in_Ecoli2.pdf" class="btn btn-primary">下载</a></p>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/81/Tongji-Notebook.png" alt="notebook">
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            <div class="caption">
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              <h5>功能验证</h5>
 +
              <p>DNS试剂反应报告</p>
 +
              <div class="nelson">
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              <p><a href="https://static.igem.org/mediawiki/2014/7/7a/Report_for_enzyme_activity.pdf" class="btn btn-primary">下载</a></p>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/81/Tongji-Notebook.png" alt="notebook">
 +
            <div class="caption">
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              <h5>抗耐高温</h5>
 +
              <p>xynB抗耐高温报告</p>
 +
              <div class="nelson">
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              <p><a href="https://static.igem.org/mediawiki/2014/7/7a/Report_for_enzyme_activity.pdf" class="btn btn-primary">下载</a></p>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/81/Tongji-Notebook.png" alt="notebook">
 +
            <div class="caption">
 +
              <h5>脱色效果验证</h5>
 +
              <p>xynB脱色效果简易验证</p>
 +
              <div class="nelson">
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              <p><a href="https://static.igem.org/mediawiki/2014/5/5e/Report_for_measuring_xynBs_bleaching_effect.pdf" class="btn btn-primary">下载</a></p>
 +
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        <!-- Contents
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            <h1>成果</h1>
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          </div>
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          <p class="lead">我们为您准备了两种版本的基因零件：其中一种是官方版本，使用了官方规定的载体。它与其他零件兼容，普世通用，但是表达效率可能比较低。另一种是我们内部优化的版本，使用pET载体。据我们实验员观察，它在BL21(DE3) <em>E.coli</em> 中表达十分高效。 <strong>您可以直接到官方基因零件部门请求我们的官方版零件，也可以直接向我们的客服代表<a id="Jin" href="#" rel="tooltip" data-placement="top" data-original-title="需收取必要的包装邮寄费，请通过这个邮箱联系我们: jiangliang2012@hotmail.com"><strong>索要</strong></a>我们的内部优化版零件。</strong>  </p>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/88/Parts-Tongji.png" alt="notebook">
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            <div class="caption">
 +
              <h5>pET22b(+)_x1 图谱</h5>
 +
              <p>装载XynB的pET22b(+)载体（无C端His-tag）</p>
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              <div class="nelson">
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                <!-- Button to trigger modal -->
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              <p><a href="#myModal" role="button" class="btn btn-primary" data-toggle="modal">预览</a></p>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/88/Parts-Tongji.png" alt="notebook">
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            <div class="caption">
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              <h5>pET22b(+)_x2 图谱</h5>
 +
              <p>装载XynB基因的pET22b(+)载体（有C端His-tag）</p>
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              <div class="nelson">
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              <p><a href="#myModal2" role="button" class="btn btn-primary" data-toggle="modal">预览</a></p>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/88/Parts-Tongji.png" alt="notebook">
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            <div class="caption">
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              <h5>pET22b(+)_ArfB 图谱</h5>
 +
              <p>装载ArfB基因的pET22b(+)载体（无C端His-tag）</p>
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              <div class="nelson">
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/88/Parts-Tongji.png" alt="notebook">
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            <div class="caption">
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              <h5>pET22b(+)_ManA1 图谱</h5>
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              <p>装载ManA1基因的pET22b(+)载体（无C端His-tag）</p>
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              <div class="nelson">
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                <!-- Button to trigger modal -->
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              <p><a href="#myModal4" role="button" class="btn btn-primary" data-toggle="modal">Preview</a></p>
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          <!-- Modal -->
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              <h3 id="myModalLabel">pET22b(+)_x1 图谱</h3>
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              <img src="https://static.igem.org/mediawiki/2014/3/3d/TongjiXynB1.png">
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              <button class="btn" data-dismiss="modal" aria-hidden="true">关闭</button>
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              <h3 id="myModalLabel">pET22b(+)_x2 图谱</h3>
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              <img src="https://static.igem.org/mediawiki/2014/6/68/TongjiXynB2.png">
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              <h3 id="myModalLabel">pET22b(+)_ArfB 图谱</h3>
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              <img src="https://static.igem.org/mediawiki/2014/8/8c/TongjiArfB.png">
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              <h3 id="myModalLabel">pET22b(+)_ManA1 图谱</h3>
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            </div>
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            <div class="modal-body">
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              <img src="https://static.igem.org/mediawiki/2014/a/a5/TongjiManA1.png">
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 +
            <div class="caption">
 +
              <h5>pSB1C3_XynB 图谱</h5>
 +
              <p>装载XynB基因的pSB1C3载体 </p>
 +
              <div class="nelson">
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                <!-- Button to trigger modal -->
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              <p><a href="#myModal5" role="button" class="btn btn-primary" data-toggle="modal">预览</a></p>
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            <div class="caption">
 +
              <h5>pSB1C3_ArfB 图谱</h5>
 +
              <p>装载ArfB基因的pSB1C3载体 </p>
 +
              <div class="nelson">
 +
                <!-- Button to trigger modal -->
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              <p><a href="#myModal6" role="button" class="btn btn-primary" data-toggle="modal">预览</a></p>
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            <div class="caption">
 +
              <h5>pSB1C3_ManA1 图谱</h5>
 +
              <p>装载ManA1基因的pSB1C3载体 </p>
 +
              <div class="nelson">
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                <!-- Button to trigger modal -->
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              <p><a href="#myModal7" role="button" class="btn btn-primary" data-toggle="modal">预览</a></p>
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              <h3 id="myModalLabel">pSB1C3_XynB 图谱</h3>
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              <h3 id="myModalLabel">pSB1C3_ArfB 图谱</h3>
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              <h3 id="myModalLabel">pSB1C3_ManA1 图谱</h3>
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Latest revision as of 03:45, 18 October 2014

iGEM Tongji 2014

实验室档案馆

方案

点击“下载”按钮，您可以查看我们的重要实验方案。

notebook
PCR方案

XynB，ArfB和ManA1的PCR方案（英文）

下载


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细菌培养方案

Clostridium stercorarium的培养方案（英文）

下载


记录

点击“下载”按钮，您可以查看我们的重要实验记录。

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载体构建

表达载体构建报告

下载


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表达验证

大肠杆菌蛋白表达报告

下载


notebook
功能验证

DNS试剂反应报告

下载


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抗耐高温

xynB抗耐高温报告

下载


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脱色效果验证

xynB脱色效果简易验证

下载


成果

我们为您准备了两种版本的基因零件：其中一种是官方版本，使用了官方规定的载体。它与其他零件兼容，普世通用，但是表达效率可能比较低。另一种是我们内部优化的版本，使用pET载体。据我们实验员观察，它在BL21(DE3) E.coli 中表达十分高效。 您可以直接到官方基因零件部门请求我们的官方版零件，也可以直接向我们的客服代表索要我们的内部优化版零件。

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pET22b(+)_x1 图谱

装载XynB的pET22b(+)载体（无C端His-tag）

预览


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pET22b(+)_x2 图谱

装载XynB基因的pET22b(+)载体（有C端His-tag）

预览


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pET22b(+)_ArfB 图谱

装载ArfB基因的pET22b(+)载体（无C端His-tag）

预览


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pET22b(+)_ManA1 图谱

装载ManA1基因的pET22b(+)载体（无C端His-tag）

Preview


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pSB1C3_XynB 图谱

装载XynB基因的pSB1C3载体

预览


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pSB1C3_ArfB 图谱

装载ArfB基因的pSB1C3载体

预览


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pSB1C3_ManA1 图谱

装载ManA1基因的pSB1C3载体

预览


同济大学, 2014

Retrieved from "http://2014.igem.org/Team:Tongji/lab-archives-zh"