Team:Hong Kong HKUST/wetlab/protocols

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<h3>Protocols</h3>
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<h3 style="text-align:center">Protocols</h3>
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<p>We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique
<p>We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique
  can be found here!
  can be found here!
</p>
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<h4> Mutagenesis</h4>
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<a href="https://static.igem.org/mediawiki/2014/1/19/Dephosphorylation.pdf">
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<p> Protocol description/picture here. </p>
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<h4> <b>Dephosphorylation</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/2/25/19._Ethanol_Precipitation.pdf">
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<h4> Western Blot </h4>
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<p> Protocol description/picture here. </p>
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<h4> <b>Ethanol Precipitation</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/6/64/Gibson_assembly_master_mix-_assembly.pdf">
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<h4> LB agar and LB plates </h4>
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<p> Protocol description/picture here. </p>
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<h4> <b>Gibson Assembly</b> </h4>
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<h4> Ligation </h4>
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<a href="https://static.igem.org/mediawiki/2014/e/e9/10._Ligation.pdf">
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<p> Protocol description/picture here. </p>
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<h4> <b>Ligation</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/f/f3/Liquid_mediums_and_Agarose_and_Plates.pdf">
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<h4> Ethanol Precipitation</h4>
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<p> Protocol description/picture here. </p>
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<h4> <b>Liquid Mediums and Agarose Plates</b> </h4>
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<p>Protocol description/picture here. </p>
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<h4> <b>M9 Minimal Medium</b> </h4>
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<h4> Gibson Assembly </h4>
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<a href="https://static.igem.org/mediawiki/2014/2/2a/Mutagenesis_protocol_1_%28multiple_sites%29.pdf">
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<p> Protocol description/picture here. </p>
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<h4> <b>Mutagenesis 1 <br>(Multiple Sites)</b></h4>
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<h4> Polymerase Chain Reaction (PCR)</h4>
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<p> Protocol description/picture here. </p>
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<h4> <b>Mutagenesis 2 <br>(Single Site)</b> </h4>
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<h4> <b>PCR (routine <i>Taq</i>)</b></h4>
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<h4> <b>PCR (Phusion)</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/c/c5/Protocol_for_Vent_PCR.pdf">
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<h4> <b>PCR (Vent)</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/7/76/RPU_Measurement_Method_.pdf">
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<h4> <b>RPU Measurement</b> </h4>
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Latest revision as of 14:52, 17 October 2014



Protocols

We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique can be found here!

Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement

Retrieved from "http://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/protocols"