Team:KIT-Kyoto/Test/Notebook/Labnote

From 2014.igem.org

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<li class="btn"><a href="">October</a></li>
<li class="btn"><a href="">October</a></li>
</ul>
</ul>
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<li class="btn"><a href="">Protcol</a></li>
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<li class="btn"><a href="/Team:KIT-Kyoto/Notebook/Protocol">Protcol</a></li>
<li class="btn"><a href="">DesignNote</a></li>
<li class="btn"><a href="">DesignNote</a></li>
</ul>
</ul>
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<table class="materials">
<table class="materials">
<tr>
<tr>
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<td rowspan="2">Host</td>
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<th rowspan="2">Host</th>
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<td colspan="2">Sample</td>
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<th colspan="2">Sample</th>
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<td rowspan="2">Enzyme</td>
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<th rowspan="2">Enzyme</th>
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<td rowspan="2">Buffer</td>
+
<th rowspan="2">Buffer</th>
</tr>
</tr>
<tr>
<tr>
-
<td>Vector</td>
+
<th>Vector</th>
-
<td>Insert</td>
+
<th>Insert</th>
</tr>
</tr>
<tr>
<tr>
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</p>
</p>
<!--本文終わり-->
<!--本文終わり-->
 +
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<!-- タイトル -->
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<h3>June 10, 2014</h3>
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<!-- タイトル終わり -->
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<!-- 本文開始 -->
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<p class="sentence">
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<!-- 実験者名 -->
 +
OKAMOTO<br>
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<!-- 実験者名終わり -->
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<!--Miniprep-->
 +
<strong>Miniprep</strong>
 +
<table class="materials">
 +
<tr>
 +
<th rowspan="2">Host</th>
 +
<th colspan="2">Sample</th>
 +
</tr>
 +
<tr>
 +
<th>Vector</th>
 +
<th>Insert</th>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</TD>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS62</td>
 +
</tr>
 +
</table>
 +
<!--Miniprep終わり-->
 +
 +
<br>
 +
 +
<!--Restriction Digest-->
 +
<strong>Restriction Digest</strong>
 +
<table class="materials">
 +
<tr>
 +
<th rowspan="2">Host</th>
 +
<th colspan="2">Sample</th>
 +
<th rowspan="2">Enzyme</th>
 +
<th rowspan="2">Buffer</th>
 +
</tr>
 +
<tr>
 +
<th>Vector</th>
 +
<th>Insert</th>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
</table>
 +
<!--Ristriction Digest-->
 +
 +
<br>
 +
</p>
 +
<!--本文終わり-->
 +

Latest revision as of 06:23, 2 October 2014

LabNote

June

June 10, 2014

OKAMOTO
Miniprep

Host Sample
Vector Insert
BL21(DE3) pGEX6P-2 CuMTSE1
BL21(DE3) pGEX6P-2 CuMTSE2
BL21(DE3) pGEX6P-2 CuMTS3
BL21(DE3) pGEX6P-2 CuMTS62

Restriction Digest
Host Sample Enzyme Buffer
Vector Insert
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 Sal1, Not1 Buffer H

June 10, 2014

OKAMOTO
Miniprep

Host Sample
Vector Insert
BL21(DE3) pGEX6P-2 CuMTSE1
BL21(DE3) pGEX6P-2 CuMTSE2
BL21(DE3) pGEX6P-2 CuMTS3
BL21(DE3) pGEX6P-2 CuMTS62

Restriction Digest
Host Sample Enzyme Buffer
Vector Insert
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 Sal1, Not1 Buffer H

July

7/25



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October




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