Team:Hong Kong HKUST/wetlab

From 2014.igem.org

(Difference between revisions)
 
(17 intermediate revisions not shown)
Line 1: Line 1:
-
<!DOCTYPE html>
+
{{Team:Hong_Kong_HKUST/shell |
<html>
<html>
<head>
<head>
-
<link rel="stylesheet" href="https://2014.igem.org/wiki/index.php?title=Template:Team:Hong_Kong_HKUST/anti-main.css&action=raw&ctype=text/css" type="text/css" >
+
-
<link rel="stylesheet" href="https://2014.igem.org/wiki/index.php?title=Template:Team:Hong_Kong_HKUST/indexpage.css&action=raw&ctype=text/css" type="text/css" >
+
<style type="text/css">
-
<link rel="stylesheet" href="https://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/Achievements_page.css&action=raw&ctype=text/css" type="text/css" >
+
-
 
+
#quick_link_wetlab {
-
<script src="http://ajax.aspnetcdn.com/ajax/jQuery/jquery-1.11.1.min.js"></script>
+
font-size:50px;
-
<script src="https://2014.igem.org/wiki/index.php?title=Template:Team:Hong_Kong_HKUST/access-menu.js&action=raw&ctype=text/css"></script>
+
text-align:center;
-
 
+
align:center;
 +
}
 +
 +
</style>
 +
 +
 +
</head>
</head>
 +
</html>|
 +
<html>
 +
<body>
<body>
-
<nav role="navigation">
+
-
<ul class="access-menu">
+
-
<li><a href="#">Home</a></li>
+
-
<li>
+
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor">Pneumosensor</a>
+
-
<ul class="access-submenu">
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/module_one">Sensing</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/module_two">Expressing</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/parts">Parts</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/data">Data</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/characterization">Characterization</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/results">Results</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/future_work">Future Work</a></li>
+
-
</ul>
+
-
</li>
+
-
<li>
+
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator">Riboregulator</a>
+
-
<ul class="access-submenu">
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/CR_TA_Feature_Page">Feature Page</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/RNA_devices_catalog">Catalog Page</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/parts">Parts</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/data">Data</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization">Characterization</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/results">Results</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/future_work">Future Work</a></li>
+
-
</ul>
+
-
</li>
+
-
<li>
+
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice">Human Practice</a>
+
-
<ul class="access-submenu">
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit">Start-up Kit</a></li>
+
-
<li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/handbook" >--Handbook</a></li>
+
-
<li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/report" >--Report</a></li>
+
-
<li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/database" >--Database</a></li>
+
-
<li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/interview" >--Interview</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/outreach">Outreach</a></li>
+
-
<li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/outreach/Workshop" >--Workshop</a></li>
+
-
<li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/outreach/talks" >--Talk</a></li>
+
-
<li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/outreach/isf_academy" >--ISF Academy</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/safety_and_ethics">Safety and Ethics</a></li>
+
-
</ul>
+
-
</li>
+
-
<li>
+
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team">Team</a>
+
-
<ul class="access-submenu">
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/members">Members</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/advisors">Advisers</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/instructors">Instructors</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/attribution">Attributions</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/acknowledgement">Acknowledgement</a></li>
+
-
<li><a href="https://igem.org/Team.cgi?year=2014">Official Team Profile</a></li>
+
-
</ul>
+
-
</li>
+
-
<li>
+
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab">Wetlab</a>
+
-
<ul class="access-submenu">
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/notebook">Notebook</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/protocols">Protocols</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/safety">Safety</a></li>
+
-
</ul>
+
-
</li>
+
-
<li>
+
-
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/Achievements">Achievements</a>
+
-
<ul class="access-submenu">
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/acheivement/medal_requirement">Medal Requirements</a></li>
+
-
<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/acheivement/deliverable">Deliverable</a></li>
+
-
</ul>
+
-
</li>
+
-
<li class="access_logo">
+
-
<a href="https://2014.igem.org/Main_Page"><img src= "https://static.igem.org/mediawiki/2014/5/55/Hkust_logo.gif"></a>
+
-
</li>
+
-
<li class="access_logo">
+
-
<a href="http://www.ust.hk/"><img src= "https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"></a>
+
-
</li>
+
-
+
-
 
+
-
</ul>
+
-
</nav>
+
-
<!-- ================ do not touch any thing above this, dont even think about it =========================-->
+
-
+
<div id="content_container">
<div id="content_container">
-
<div class= "banner_area">
+
<div class='content_1'>
-
<img src= 'https://static.igem.org/mediawiki/2014/3/3c/HKUST_2014_pneumosensor_banner.jpg' />
+
<h3 style="text-align:center">Wetlab</h3>
-
<!--banner should be larger and height should be more than 400px-->
+
<table class="content_table" align= "center" valign= "top">
-
</div>
+
<tr class= "content_row">
-
<hr>
+
<td class= "content_cell">
-
<div id="description_area">
+
<div class= "content_area_one_row">
-
<h2>Wetlab</h2>
+
<p>The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely:  
<p>The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely:  
-
(1) (Detecting Module) ComD & ComE, (2) (Detecting Module) P<sub><i>comCDE</i></sub>, (3) (Regulation Module) ComX & ComW, and  
+
(1) (Detection Module) ComD & ComE, (2) (Detection Module) P<sub><i>comCDE</i></sub>, (3) (&sigma;<sup>X</sup> promoters Module) &sigma;<sup>X</sup> & ComW, and  
-
(4) (Regulation Module) combox. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively  
+
(4) (&sigma;<sup>X</sup> promoters Module) P<sub>celA</sub> and P<sub>comFA</sub>. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively  
and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows.  
and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows.  
-
The BioBrick, researcher, environmental and university safety issues can be found in Safety page. In terms of project process,
+
The BioBrick, researchers, environmental and university safety issues can be found in Safety page. In terms of project process,
some experiments could not be completed due to time constrains and they are outlined in Future Work page.  
some experiments could not be completed due to time constrains and they are outlined in Future Work page.  
</p>
</p>
 +
</div>
 +
</td>
 +
</tr>
 +
</table>
-
</div>
 
<div id= 'project_buttons'>
<div id= 'project_buttons'>
<table id = "project_buttons_table" align= "center" >
<table id = "project_buttons_table" align= "center" >
-
<tr class= "start_up_link_row">
+
<tr class= "start_up_link_row" align="center">
-
+
<td class= "start_up_link_button">
-
<td class= "start_up_link_button">
+
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/notebook"><div class= "start_up_link_container">  
-
<a href="#"><div class= "start_up_link_container">  
+
<div id="quick_link_wetlab">
-
<h4> Protocol</h4>
+
Notebook
-
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
+
<hr>
-
visibly touching the cheekbone below the eye. </p>
+
<img src="https://static.igem.org/mediawiki/2014/2/28/NotebookHKUST.png" width="200" height="200">
 +
</div>
</div>
</div>
</td>
</td>
-
<td class= "start_up_link_button">
+
<td class= "start_up_link_button">
-
<a href="#"><div class= "start_up_link_container">  
+
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/protocols"><div class= "start_up_link_container">  
-
<h4> Notebook </h4>
+
<div id="quick_link_wetlab">
-
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
+
Protocols
-
visibly touching the cheekbone below the eye. </p>
+
<hr>
 +
<img src="https://static.igem.org/mediawiki/2014/2/26/HKUST_Quicklink_Icon_Protocols.png" width="200" height="200">
 +
</div>
 +
</div>
</div>
</td>
</td>
<td class= "start_up_link_button">
<td class= "start_up_link_button">
-
<a href="#"><div class= "start_up_link_container">
+
<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/safety"><div class= "start_up_link_container">
-
<h4> Safety </h4>
+
<div id="quick_link_wetlab">
-
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
+
Safety
-
visibly touching the cheekbone below the eye. </p>
+
<hr>
 +
<img src="https://static.igem.org/mediawiki/2014/e/e1/HKUST_Quicklink_Icon_Safety.png" width="200" height="200">
 +
</div>
 +
</div>
</div>
</td>
</td>
Line 141: Line 80:
</table>
</table>
</div>
</div>
 +
<hr>
<hr>
-
+
</div>
-
<table class= "site_map_table" align= "center">
+
</div>
-
<tr>
+
-
<td class= 'site_map_column'><a href="#"><h4>Home</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Team</li></a>
+
-
<li class='site_link'><a href="#">Project</li></a>
+
-
<li class='site_link'><a href="#">WetLab</li></a>
+
-
<li class='site_link'><a href="#">Achievement</li></a>
+
-
<li class='site_link'><a href="#">Human Practice</li></a>
+
-
<li class='site_link'><a href="#">Side Project</li></a>
+
-
</ul>
+
-
 
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Team</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Member</li></a>
+
-
<li class='site_link'><a href="#">Advisers</li></a>
+
-
<li class='site_link'><a href="#">Instructor</li></a>
+
-
<li class='site_link'><a href="#">Attribution</li></a>
+
-
<li class='site_link'><a href="#">Acknowledgement</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Main Project</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Overview</li></a>
+
-
<li class='site_link'><a href="#">Module one</li></a>
+
-
<li class='site_link'><a href="#">Module two</li></a>
+
-
<li class='site_link'><a href="#">Data</li></a>
+
-
<li class='site_link'><a href="#">Characterisation</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Side Project</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Overview</li></a>
+
-
<li class='site_link'><a href="#">Parts</li></a>
+
-
<li class='site_link'><a href="#">Catalogue</li></a>
+
-
<li class='site_link'><a href="#">Feature Pages</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Human Practice</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Overview</li></a>
+
-
<li class='site_link'><a href="#">Start up kit</li></a>
+
-
<ul>
+
-
<li class='site_link'><a href="#">Interview</li></a>
+
-
<li class='site_link'><a href="#">Report</li></a>
+
-
<li class='site_link'><a href="#">Database</li></a>
+
-
</ul>
+
-
<li class='site_link'><a href="#">Talks</li></a>
+
-
<li class='site_link'><a href="#">Outreach</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Wetlab</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Protocol</li></a>
+
-
<li class='site_link'><a href="#">Notebook</li></a>
+
-
<li class='site_link'><a href="#">Safety</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Achievement</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Medal Requirements</li></a>
+
-
<li class='site_link'><a href="#">Deliverable</li></a>
+
-
</ul>
+
-
</td>
+
-
</tr>
+
-
 
+
-
</table>
+
</body>
</body>
-
</html>
+
</html>  
 +
}}

Latest revision as of 22:01, 17 October 2014



Wetlab

The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely: (1) (Detection Module) ComD & ComE, (2) (Detection Module) PcomCDE, (3) (σX promoters Module) σX & ComW, and (4) (σX promoters Module) PcelA and PcomFA. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows. The BioBrick, researchers, environmental and university safety issues can be found in Safety page. In terms of project process, some experiments could not be completed due to time constrains and they are outlined in Future Work page.


Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement