Team:Hong Kong HKUST/wetlab/protocols

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<li><a href="#">Home</a></li>
 
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<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor">Pneumosensor</a>
 
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<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/module_one">Sensing</a></li>
 
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<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator">Riboregulator</a>
 
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<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization">Characterization</a></li>
 
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<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/results">Results</a></li>
 
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<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab">Wetlab</a>
 
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<h3 style="text-align:center">Protocols</h3>
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<h2>Wetlab</h2>
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<p>We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique
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<p>The wet lab started at the beginning of summer and lasted for three months. Our wet lab work is divided into four parts, namely:
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  can be found here!
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(1) (Detecting Module) ComD & ComE, (2) (Detecting Module) P<sub><i>comCDE</i></sub>, (3) (Regulation Module) ComX & ComW, and
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(4) (Regulation Module) combox. During the summer, lab work such as primer design, digestion, and ligation was carried out extensively
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and useful data were collected. These can be retrieved from Notebook and Protocols pages for details of experimental flows.
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The BioBrick, researcher, environmental and university safety issues can be found in Safety page. In terms of project process,
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some experiments could not be completed due to time constrains and they are outlined in Future Work page.
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<h4> Protocol</h4>
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<h4> <b>Dephosphorylation</b> </h4>
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<h4> <b>Ethanol Precipitation</b> </h4>
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<h4> <b>Gibson Assembly</b> </h4>
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<h4> <b>Ligation</b> </h4>
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<h4> <b>M9 Minimal Medium</b> </h4>
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<h4> <b>Mutagenesis 1 <br>(Multiple Sites)</b></h4>
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<h4> <b>PCR (routine <i>Taq</i>)</b></h4>
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<h4> <b>PCR (Vent)</b> </h4>
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<h4> <b>RPU Measurement</b> </h4>
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<td class= 'site_map_column'><a href="#"><h4>Home</h4></a>
 
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<li class='site_link'><a href="#">Team</li></a>
 
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<li class='site_link'><a href="#">Project</li></a>
 
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<li class='site_link'><a href="#">WetLab</li></a>
 
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<li class='site_link'><a href="#">Achievement</li></a>
 
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<li class='site_link'><a href="#">Human Practice</li></a>
 
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<li class='site_link'><a href="#">Side Project</li></a>
 
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<td class= 'site_map_column'><a href="#"><h4>Team</h4></a>
 
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<li class='site_link'><a href="#">Member</li></a>
 
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<li class='site_link'><a href="#">Advisers</li></a>
 
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<li class='site_link'><a href="#">Instructor</li></a>
 
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<li class='site_link'><a href="#">Attribution</li></a>
 
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<li class='site_link'><a href="#">Acknowledgement</li></a>
 
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<td class= 'site_map_column'><a href="#"><h4>Main Project</h4></a>
 
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<li class='site_link'><a href="#">Overview</li></a>
 
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<li class='site_link'><a href="#">Module one</li></a>
 
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<li class='site_link'><a href="#">Module two</li></a>
 
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<li class='site_link'><a href="#">Data</li></a>
 
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<li class='site_link'><a href="#">Parts</li></a>
 
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<li class='site_link'><a href="#">Catalogue</li></a>
 
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<li class='site_link'><a href="#">Feature Pages</li></a>
 
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<li class='site_link'><a href="#">Report</li></a>
 
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<li class='site_link'><a href="#">Talks</li></a>
 
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<li class='site_link'><a href="#">Medal Requirements</li></a>
 
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Latest revision as of 14:52, 17 October 2014



Protocols

We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique can be found here!

Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement

Retrieved from "http://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/protocols"