Team:Hong Kong HKUST/pneumosensor

From 2014.igem.org

(Difference between revisions)
(Created page with "<html> <head> <link rel="stylesheet" type="text/css" href="https://2014.igem.org/wiki/index.php?title=Template:Team:Hong_Kong_HKUST/indexpage.css&action=raw&ctype=text...")
 
(82 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:Hong_Kong_HKUST/shell|
<html>
<html>
<head>
<head>
-
+
<style type="text/css">
-
<link rel="stylesheet" type="text/css" href="https://2014.igem.org/wiki/index.php?title=Template:Team:Hong_Kong_HKUST/indexpage.css&amp;action=raw&amp;ctype=text/css">
+
div.quick_link_container{
-
<script src="http://ajax.aspnetcdn.com/ajax/jQuery/jquery-1.11.1.min.js"></script>
+
width: 134px;
-
<script>
+
height: 160px;
-
$(document).ready(function(){
+
}
-
  $(window).scroll(function(){
+
div.quick_link_container img{
-
var scrollTop = $(window).scrollTop();
+
width:100%;
-
if (scrollTop > 49) {
+
}
-
$('#menu').addClass('fixed');
+
.quick_link_container > h4 {
-
} else {
+
font-size: 13px;
-
$('#menu').removeClass('fixed');
+
}
-
}
+
div.quick_link_row div{
-
  });
+
width:180px;
-
});
+
}
-
 
+
</style>
-
</script>
+
-
+
-
<script>
+
-
var on = true;
+
-
var myVar = setInterval(function(){ if(on) {slides()}on=true;},3000);
+
-
 
+
-
function slides(i) {
+
-
  document.getElementsByClassName("slide_pic")[1].style.width="0px";
+
-
  document.getElementsByClassName("slide_pic")[2].style.width="100%";
+
-
  var slide = document.getElementsByClassName("slide_pic")[0];
+
-
  document.getElementById("slide").appendChild(slide);
+
-
  if(i) {
+
-
  on = false;
+
-
  }
+
-
}
+
-
function back_slide(i) {
+
-
  document.getElementsByClassName("slide_pic")[1].style.width="0px";
+
-
  document.getElementsByClassName("slide_pic")[0].style.width="100%";
+
-
  var slide = document.getElementsByClassName("slide_pic")[4];
+
-
  var slide2 = document.getElementsByClassName("slide_pic")[0];
+
-
  document.getElementById("slide").insertBefore(slide,slide2);
+
-
  if(i) {
+
-
  on = false;
+
-
  }
+
-
}
+
-
</script>
+
</head>
</head>
-
+
</html>
-
<body>
+
|
-
<div id='nav_container'>
+
<html><body>
-
<nav>
+
-
+
-
<ul id="menu">
+
-
<li><a href="#">Home</a></li>
+
-
        <li><a href="#">Team</a>
+
-
        <!-- First Tier Drop Down -->
+
-
        <ul>
+
-
            <li><a href="#">Members</a></li>
+
-
            <li><a href="#">Advisers</a></li>
+
-
            <li><a href="#">Instructor</a></li>
+
-
        </ul>       
+
-
        </li>
+
-
        <li><a href="#">Project</a>
+
-
        <!-- First Tier Drop Down -->
+
-
        <ul>
+
-
            <li><a href="#">Main Project</a>
+
-
<ul>
+
-
                <li><a href="#">Overview</a></li>
+
-
                <li><a href="#">Module one </a></li>
+
-
                <li><a href="#">Module two</a></li>
+
-
<li><a href="#">Data</a></li>
+
-
<li><a href="#">Characterization</a></li>
+
-
            </ul></li>
+
-
            <li><a href="#">Side Project</a>
+
-
<ul>
+
-
                <li><a href="#">Overview</a></li>
+
-
                <li><a href="#">Parts</a></li>
+
-
                <li><a href="#">Catalogue</a></li>
+
-
<li><a href="#">Feature pages</a></li>
+
-
            </ul></li>
+
-
       
+
-
        </ul>
+
-
        </li>
+
-
        <li><a href="#">Human Practice</a>
+
-
<ul>
+
-
                <li><a href="#">Overview</a></li>
+
-
                <li><a href="#">Start up kit</a>
+
-
<ul>
+
-
<li><a href="#">Interview</a></li>
+
-
<li><a href="#">Report</a></li>
+
-
<li><a href="#">Database</a></li>
+
-
</ul></li>
+
-
                <li><a href="#">Talks</a></li>
+
-
<li><a href="#">Outreach</a></li>
+
-
            </ul></li>
+
-
        <li><a href="#">wetlab</a>
+
-
<ul>
+
-
<li><a href="#">Protocol</a></li>
+
-
<li><a href="#">Notebook</a></li>
+
-
<li><a href="#">Safety</a></li>
+
-
</ul></li>
+
-
        <li><a href="#">Achievement</a>
+
-
<ul>
+
-
<li><a href="#">Medal Requirement</a></li>
+
-
<li><a href="#">Deliverable</a></li>
+
-
</ul></li>
+
-
<li class= "logo"><a href="https://2014.igem.org/Main_Page"> <img src="Igemlogo_whiteletters.png" height="45px"> </a> </li>
+
-
<li class= "logo"><a href="http://www.ust.hk/"> <img src="HKUST_logo.png" height="45px"> </a></li>
+
-
+
-
</ul>
+
-
+
-
</nav>
+
-
</div>
+
-
<h1> </h1>
+
-
<!-- ================ do not touch any thing above this, dont even think about it =========================-->
+
-
+
<div id="content_container">
<div id="content_container">
-
<div class= "banner_area">
+
<div class="quick_link_area">
-
<img src= 'banner_mod.png' />
+
-
<!--banner should be larger and height should be more than 400px-->
+
-
</div>
+
-
<hr>
+
 +
<div class= "quick_link_row">
 +
<div>
 +
<a class="quick_link_sub" href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/modules">
 +
<img src="https://static.igem.org/mediawiki/2014/thumb/f/ff/HKUST_Quicklink_Icon_Module.png/610px-HKUST_Quicklink_Icon_Module.png"/>
 +
                                                <p>Modules Description</p>
 +
</a>
 +
</div>
 +
<div>
 +
<a class="quick_link_sub" href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/parts">
 +
<img src="https://static.igem.org/mediawiki/2014/thumb/6/6b/Part_final_final.png/604px-Part_final_final.png"/>
 +
                                                <p>Parts</p>
 +
</a>
 +
</div>
 +
<!--<div>
 +
<a class="quick_link_sub" href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/data">
 +
<img src="https://static.igem.org/mediawiki/2014/4/42/HKUST_Quicklink_Icon_Data.png"/>
 +
                                                <p>Data</p>
 +
</a>
 +
</div>
 +
</div>
 +
<div class= "quick_link_row">-->
 +
<div>
 +
<a class="quick_link_sub" href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/characterization">
 +
<img src="https://static.igem.org/mediawiki/2014/thumb/f/fe/CharacterizationHKUST.png/612px-CharacterizationHKUST.png"/>
 +
                                                <p>Characterization</p>
 +
 +
</a>
 +
</div>
 +
<div>
 +
<a class="quick_link_sub" href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/results">
 +
<img src="https://static.igem.org/mediawiki/2014/thumb/2/27/Results.HKUST2014.png/610px-Results.HKUST2014.png"/>
 +
                                                <p>Results</p>
 +
</a>
 +
</div>
 +
<div>
 +
<a class="quick_link_sub" href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/future_work">
 +
<img src="https://static.igem.org/mediawiki/2014/thumb/4/49/HKUST_Quicklink_Icon_Future_Work.png/612px-HKUST_Quicklink_Icon_Future_Work.png"/>
 +
                                                <p>Future Work</p>
 +
 +
</a>
 +
</div>
 +
</div>
-
<!--two row of content, image first row, text second row -->
+
</div>
-
<div class='content_1'><h3> Pneumosensor Project Abstract </h3>
+
 +
 
 +
<!-- one row of content , two column one picture left-->
 +
<div class='content_1'><h3>Project Pneumosensor Abstract </h3>
<table class="content_table" align= "center" >
<table class="content_table" align= "center" >
-
<tr class= "content_row" >
+
<tr class= "content_row" valign= "top">
-
<td class= "content_cell" colspan= "2">
+
<td class= "content_cell">
-
<div class= "content_area_two_row">
+
<div class= "content_area_one_row">
<div class="content_image">
<div class="content_image">
-
<img src= "potato.jpg"/>
+
<img src= "https://static.igem.org/mediawiki/2014/thumb/2/27/XXX_with_partsHKUST.jpg/800px-XXX_with_partsHKUST.jpg"/>
-
<h5>Fig 1 . Here is the potato.</h5>
+
<h6><b>Figure 1. Pneumosensor Overview Diagram</h6></b><br>
-
<h6> Here is the description of the potato: it is a potato!</h6>
+
<h7> Quorem-sensing peptide CSP produced by <i>S. pneumoniae</i> is detected by the Detection Module; the response regulator ComE links the Detection Module to the &sigma;<sup>x</sup> Promoters Module which ultimately drives the expression of GFP. </h7>
</div>
</div>
</div>
</div>
</td>
</td>
-
</tr>
 
-
<tr class= "content_row">
+
<td class= "content_cell">
-
<td class= "content_cell">
+
<div class= "content_area_one_row">
-
<div class= "content_area_two_row">
+
<p class="first_letter_enhanced"><i>Streptococcus pneumoniae</i> is responsible for approximately 1.6 million deaths every year mostly in developing countries where vaccination  
-
<p><i>Streptococcus pneumoniae</i> is responsible for approximately 1.6 million deaths every year mostly in developing countries where vaccination  
+
is unaffordable (World Health Organization, 2014). Current methods used for diagnosis of pneumococcal diseases include X-ray imaging of chest, PCR assay using blood and Gram stain using sputum (Muench, D. F., 2014). They require sophisticated equipments, help from physicians or laboratory settings, all of which are limited to people in third world countries. To provide an affordable solution to combat pneumococcal diseases, we are engineering an <i>E. coli</i> &quot;Pneumosensor&quot;, that will  
-
is unaffordable. To provide an affordable solution to combat pneumococcal diseases, we are engineering an <i>E. coli</i> &quot;Pneumosensor&quot;, that will  
+
work inside as an <i>in vitro</i> diagnostic device. Such a device should provide diagnosis result in absence of laboratories, medical equipments or doctors, making it more accessible for the people. Through this cheaper and easier diagnosis method, people can prepare themselves better against the  
-
work inside an in vitro diagnostic device. Through this cheaper and easier diagnosis method, people can prepare themselves better against the  
+
transmission and adversity of <i>S. pneumoniae</i>.<br><br>
-
transmission and adversity of <i>S. Pneumoniae</i>. </p>
+
Pneumosensor has a rewired <i>comCDE</i> signal transduction pathway native to <i>S. pneumoniae</i> that detects
-
</div>
+
autoinducer molecules released specifically by <i>S. pneumoniae</i>. Upon detection of <i>Streptococcus pneumonia</i>, Pneumosensor will produce a fluorescence signal. Apart from that, Pneumosensor is also equipped with a new promoter, Com-Box promoter, for the specific and tight regulation of target gene expression in <i>E. coli</i>. </p>
-
</td>
+
</div>
-
<td class= "content_cell">
+
</td>
-
<div class= "content_area_two_row">
+
-
<p>Pneumosensor has a rewired comCDE signal transduction pathway native to <i>S. pneumoniae</i> that detects
+
-
autoinducer molecules released specifically by <i>S. pneumoniae</i>. It is also equipped with a new promoter for the specific and tight regulation of  
+
-
target gene expression in <i>E. coli</i>. In addition, the team is documenting and characterizing existing CR-TA riboregulators for post-transcriptional
+
-
regulation of gene expression. Our work will help other iGEM teams more effectively incorporate this tuning tool into their project design. </p>
+
-
</div>
+
-
</td>
+
 +
</tr>
 +
</div>
 +
</table>
 +
</div>
 +
<br><br>
 +
<!-- end of one row of content , two column one picture left-->
 +
<div class='content_1'>
 +
<table class="content_table" align= "center" >
 +
<tr class= "content_row" >
 +
<td class= "content_cell" colspan= "2">
 +
<div class= "content_area_two_row">
 +
<p>
 +
<u>References</u>
 +
<br>
 +
<br>
 +
Muench, D. F. (2014). Pneumococcal Infections. Retrieved from: http://emedicine.medscape.com/article/225811-overview. [Last Accessed 17, October 2014].
 +
<br>
 +
<br>World Health Organization (2014). Pneumococcal disease. [ONLINE] Retrieved from: http://www.who.int/ith/diseases/pneumococcal/en/. [Last Accessed 17 October 14].
 +
<br>
 +
<br>
 +
</p>
 +
</div>
 +
</td>
</tr>
</tr>
</table>
</table>
</div>
</div>
-
<!--end of two row of content, image first row, text second row -->
 
-
<div id= 'project_buttons'>
 
-
<table id = "project_buttons_table" align= "center" >
 
-
<tr class= "start_up_link_row">
 
-
 
-
<td class= "start_up_link_button">
 
-
<a href="#"><div class= "start_up_link_container">
 
-
<h4> Modules Description </h4>
 
-
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
 
-
visibly touching the cheekbone below the eye. </p>
 
-
</div>
 
-
</td>
 
-
 
-
<td class= "start_up_link_button">
 
-
<a href="#"><div class= "start_up_link_container">
 
-
<h4> Data Page </h4>
 
-
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
 
-
visibly touching the cheekbone below the eye. </p>
 
-
</div>
 
-
</td>
 
-
 
-
<td class= "start_up_link_button">
 
-
<a href="#"><div class= "start_up_link_container">
 
-
<h4> Parts </h4>
 
-
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
 
-
visibly touching the cheekbone below the eye. </p>
 
-
</div>
 
-
</td>
 
-
</tr>
 
-
 
-
<tr class= "start_up_link_row">
 
-
<td class= "start_up_link_button">
 
-
<a href="#"><div class= "start_up_link_container">
 
-
<h4> Results </h4>
 
-
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
 
-
visibly touching the cheekbone below the eye. </p>
 
-
</div>
 
-
</td>
 
-
 
-
<td class= "start_up_link_button">
 
-
<a href="#"><div class= "start_up_link_container">
 
-
<h4> Characterisation </h4>
 
-
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
 
-
visibly touching the cheekbone below the eye. </p>
 
-
</div>
 
-
</td>
 
-
 
-
<td class= "start_up_link_button">
 
-
<a href="#"><div class= "start_up_link_container">
 
-
<h4> Future Work </h4>
 
-
<p> Cheeks are fleshy in humans and other mammals, the skin being suspended by the chin and the jaws, and forming the lateral wall of the human mouth,
 
-
visibly touching the cheekbone below the eye. </p>
 
-
</div>
 
-
</td>
 
-
</tr>
 
-
 
-
</table>
 
-
</div>
 
-
 
-
<hr>
 
-
<table class= "site_map_table" align= "center">
+
</div>
-
<tr>
+
</div>
-
<td class= 'site_map_column'><a href="#"><h4>Home</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Team</li></a>
+
-
<li class='site_link'><a href="#">Project</li></a>
+
-
<li class='site_link'><a href="#">WetLab</li></a>
+
-
<li class='site_link'><a href="#">Achievement</li></a>
+
-
<li class='site_link'><a href="#">Human Practice</li></a>
+
-
<li class='site_link'><a href="#">Side Project</li></a>
+
-
</ul>
+
-
 
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Team</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Member</li></a>
+
-
<li class='site_link'><a href="#">Advisor</li></a>
+
-
<li class='site_link'><a href="#">Instructor</li></a>
+
-
<li class='site_link'><a href="#">Attribution</li></a>
+
-
<li class='site_link'><a href="#">Acknowledgement</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Main Project</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Overview</li></a>
+
-
<li class='site_link'><a href="#">Module one</li></a>
+
-
<li class='site_link'><a href="#">Module two</li></a>
+
-
<li class='site_link'><a href="#">Data</li></a>
+
-
<li class='site_link'><a href="#">Characterisation</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Side Project</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Overview</li></a>
+
-
<li class='site_link'><a href="#">Parts</li></a>
+
-
<li class='site_link'><a href="#">Catalogue</li></a>
+
-
<li class='site_link'><a href="#">Feature Pages</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Human Practice</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Overview</li></a>
+
-
<li class='site_link'><a href="#">Start up kit</li></a>
+
-
<ul>
+
-
<li class='site_link'><a href="#">Interview</li></a>
+
-
<li class='site_link'><a href="#">Report</li></a>
+
-
<li class='site_link'><a href="#">Database</li></a>
+
-
</ul>
+
-
<li class='site_link'><a href="#">Talks</li></a>
+
-
<li class='site_link'><a href="#">Outreach</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Wetlab</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Protocol</li></a>
+
-
<li class='site_link'><a href="#">Notebook</li></a>
+
-
<li class='site_link'><a href="#">Safety</li></a>
+
-
</ul>
+
-
</td>
+
-
<td class= 'site_map_column'><a href="#"><h4>Achievement</h4></a>
+
-
<ul class= 'site_list'>
+
-
<li class='site_link'><a href="#">Medal Requirements</li></a>
+
-
<li class='site_link'><a href="#">Deliverable</li></a>
+
-
</ul>
+
-
</td>
+
-
</tr>
+
-
 
+
-
</table>
+
 +
</body>
</body>
</html>
</html>
 +
}}

Latest revision as of 19:39, 17 October 2014



Project Pneumosensor Abstract

Figure 1. Pneumosensor Overview Diagram

Quorem-sensing peptide CSP produced by S. pneumoniae is detected by the Detection Module; the response regulator ComE links the Detection Module to the σx Promoters Module which ultimately drives the expression of GFP.

Streptococcus pneumoniae is responsible for approximately 1.6 million deaths every year mostly in developing countries where vaccination is unaffordable (World Health Organization, 2014). Current methods used for diagnosis of pneumococcal diseases include X-ray imaging of chest, PCR assay using blood and Gram stain using sputum (Muench, D. F., 2014). They require sophisticated equipments, help from physicians or laboratory settings, all of which are limited to people in third world countries. To provide an affordable solution to combat pneumococcal diseases, we are engineering an E. coli "Pneumosensor", that will work inside as an in vitro diagnostic device. Such a device should provide diagnosis result in absence of laboratories, medical equipments or doctors, making it more accessible for the people. Through this cheaper and easier diagnosis method, people can prepare themselves better against the transmission and adversity of S. pneumoniae.

Pneumosensor has a rewired comCDE signal transduction pathway native to S. pneumoniae that detects autoinducer molecules released specifically by S. pneumoniae. Upon detection of Streptococcus pneumonia, Pneumosensor will produce a fluorescence signal. Apart from that, Pneumosensor is also equipped with a new promoter, Com-Box promoter, for the specific and tight regulation of target gene expression in E. coli.



References

Muench, D. F. (2014). Pneumococcal Infections. Retrieved from: http://emedicine.medscape.com/article/225811-overview. [Last Accessed 17, October 2014].

World Health Organization (2014). Pneumococcal disease. [ONLINE] Retrieved from: http://www.who.int/ith/diseases/pneumococcal/en/. [Last Accessed 17 October 14].

Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement

Retrieved from "http://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor"