Team:UFAM Brazil/7-7-2014

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<p>The inoculum in liquid media grew and we extracted plasmidial DNA from JM110 and C2925 with pUC72.</p>
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<p>The inoculum in liquid medium grew and we extracted plasmidial DNA from JM110 and C2925 with pUC72.</p>
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<p>We got the DNA diluted from the 2013 DNA kit (Parts Kit) the following biobricks to perform a transformation: BBa_E0840 e BBa_K346004. </p>
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<p>We got the DNA diluted from the 2013 DNA kit (Parts Kit). Of the following biobricks, to perform the transformation: BBa_E0840 and BBa_K346004. </p>
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<p> We transformed JM110 by electroporation– and did a wash with sorbitol 1M – and commercial DH10B with the following biobricks: BBa_E0840 e BBa_K346004.</p>
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<p> We transformed JM110 by electroporation – and washed it with sorbitol 1M – and commercial DH10B with the following biobricks: BBa_E0840 and BBa_K346004.</p>
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Latest revision as of 23:13, 16 October 2014

07/07/2014

The inoculum in liquid medium grew and we extracted plasmidial DNA from JM110 and C2925 with pUC72.

We got the DNA diluted from the 2013 DNA kit (Parts Kit). Of the following biobricks, to perform the transformation: BBa_E0840 and BBa_K346004.

We transformed JM110 by electroporation – and washed it with sorbitol 1M – and commercial DH10B with the following biobricks: BBa_E0840 and BBa_K346004.

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