Team:UFAM Brazil/7-5-2014

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<tr><td><h1>07/04/2014</h1></td></tr>
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<tr><td><h1>07/05/2014</h1></td></tr>
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Results with the antibiotics tests:
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Results of the antibiotics tests:
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<img src="https://static.igem.org/mediawiki/2014/6/6a/UFAM_Brazil_2014_7-5-2014.jpg" width="400" heigth="500" >
<img src="https://static.igem.org/mediawiki/2014/6/6a/UFAM_Brazil_2014_7-5-2014.jpg" width="400" heigth="500" >
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<p><b>Legend</b></p>
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<p style="background-color:green ">Green: it grew.</p>
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<p style="background-color:yellow">Yellow: It didn’t grow at a good amount</p>
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<p style="background-color:red">Red: It didn’t grow</p>
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<td><p><b>Legend</b></p><p style="background-color:green">Green: it grew.</p><p style="background-color:yellow">Yellow: It didn’t grow at a good amount</p><p style="background-color:red">Red: It didn’t grow</p></td>
 
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<tr><td  colspan="3" align="center"><p> Pictures of our trials using antibiotic in the JM110 e ERC2925 E.coli lines
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<tr><td  colspan="3" align="center"><p> Pictures of our trials using antibiotic on JM110 and ERC2925 <i>E. coli</i>
ER5C2925 – Trial with Streptomycin, in order: <i>20 ug/ml, 40 ug/ml, 100 ug/ml and 150 ug/ml</i>:
ER5C2925 – Trial with Streptomycin, in order: <i>20 ug/ml, 40 ug/ml, 100 ug/ml and 150 ug/ml</i>:
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<tr><td  colspan="3" align="center"><p> ER5C2925 – Trials with Ampicillin: <i>50ug/ml and100ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p> ER5C2925 – Trials with Ampicillin: <i>50ug/ml and 100ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p> ER5C2925 – Trials with Tetracycline :<i> 12 ug/ml and 24 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p> ER5C2925 – Trials with Tetracycline:<i> 12 ug/ml and 24 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p> ER5C2925 – Trials with Kanamycin: <i>25 ug/ml e 50 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p> ER5C2925 – Trials with Kanamycin: <i>25 ug/ml and 50 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p> ER5C2925 – Trials with <i>Chloramphenicol: 34 ug/ml and 68 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p> ER5C2925 – Trials with Chloramphenicol: <i>34 ug/ml and 68 ug/ml</i>:</p>
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</td></tr>
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<tr><td  colspan="3" align="center"><p>JM110 – Trials with <i>Streptomycin: 20 ug/ml, 40 ug/ml, 100 ug/ml and 150 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p>JM110 – Trials with Streptomycin: <i>20 ug/ml, 40 ug/ml, 100 ug/ml and 150 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p>JM110 – Trials with<i> Tetraciclyn:12 ug/ml e 24 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p>JM110 – Trials with Tetraciclyn: <i>12 ug/ml and 24 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p>JM110 – Trial with <i>Ampicylin 50 ug/ml 100 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p>JM110 – Trial with Ampicylin <i>50 ug/ml and 100 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p>JM110 – Trial with <i>Kanamycin: 25 ug/ml and 50 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p>JM110 – Trial with Kanamycin: <i>25 ug/ml and 50 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p>JM110 – Trials with <i>chloramphenicol: 34 ug/ml and 68 ug/ml</i>:</p>
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<tr><td  colspan="3" align="center"><p>JM110 – Trials with chloramphenicol: <i>34 ug/ml and 68 ug/ml</i>:</p>
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<tr><td  colspan="3" ><p>Today we also cryopreserved the bacteria with glycerol and we inoculated LB soft medium to store them.</p>
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<tr><td  colspan="3" ><p>Today we also cryopreserved the bacteria with glycerol and was inoculated in LB soft medium to store them.</p>
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<p> We transformed using the Transformation Protocol using Sorbitol in the li <i>E. coli</i> JM110 e ERC2925 lines with the pUC72 plasmid. </p>
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<p> We transformed using the Transformation Protocol, using Sorbitol on <i>E. coli</i> JM110 and ERC2925 with the pUC72 plasmid. </p>
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Latest revision as of 23:01, 16 October 2014

07/05/2014

Results of the antibiotics tests:

Legend

Green: it grew.

Yellow: It didn’t grow at a good amount

Red: It didn’t grow

Pictures of our trials using antibiotic on JM110 and ERC2925 E. coli ER5C2925 – Trial with Streptomycin, in order: 20 ug/ml, 40 ug/ml, 100 ug/ml and 150 ug/ml:

ER5C2925 – Trials with Ampicillin: 50ug/ml and 100ug/ml:

ER5C2925 – Trials with Tetracycline: 12 ug/ml and 24 ug/ml:

ER5C2925 – Trials with Kanamycin: 25 ug/ml and 50 ug/ml:

ER5C2925 – Trials with Chloramphenicol: 34 ug/ml and 68 ug/ml:

JM110 – Trials with Streptomycin: 20 ug/ml, 40 ug/ml, 100 ug/ml and 150 ug/ml:

JM110 – Trials with Tetraciclyn: 12 ug/ml and 24 ug/ml:

JM110 – Trial with Ampicylin 50 ug/ml and 100 ug/ml:

JM110 – Trial with Kanamycin: 25 ug/ml and 50 ug/ml:

JM110 – Trials with chloramphenicol: 34 ug/ml and 68 ug/ml:

Today we also cryopreserved the bacteria with glycerol and was inoculated in LB soft medium to store them.

We transformed using the Transformation Protocol, using Sorbitol on E. coli JM110 and ERC2925 with the pUC72 plasmid.

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