Team:UESTC-China

From 2014.igem.org

(Difference between revisions)
(Undo revision 135695 by Zhangmanni (talk))
 
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<head>
<head>
<meta http-equiv="Content-Type" content="text/html;charset=UTF-8" />
<meta http-equiv="Content-Type" content="text/html;charset=UTF-8" />
-
<title>UESTC-China</title>
+
<title>Plant Vs HCHO</title>
 +
 
 +
 
<style>
<style>
 +
.imageRotation{
 +
height:500px;
 +
width:1195px;
 +
overflow:hidden;  /*--超出容器的所有元素都不可见--*/
 +
position:relative;  /*--相对定位--*/
 +
border:10px solid #eee;
 +
bodrer-radius:5px;
 +
-webkit-border-radius:5px;
 +
-moz-border-radius:5px;
 +
}
 +
/*-------------图片容器---------------*/
 +
.imageBox{
 +
position:absolute;  /*--固定定位--*/
 +
height:500px;
 +
top:0px;
 +
left:0px;
 +
overflow:hidden;
 +
}
 +
.imageBox img {
 +
display:block;
 +
height:500px;
 +
width:1195px;
 +
float:left;
 +
border:none;
 +
}
 +
/*-------------标题容器---------------*/
 +
.titleBox{
 +
position:absolute;
 +
bottom:0px;
 +
width:1214px;
 +
height:40px;
 +
overflow:hidden;
 +
}
 +
.titleBox p{
 +
position:absolute;
 +
bottom:-40px;
 +
width:1214px;
 +
height:40px;
 +
margin:0px;
 +
padding:0px 10px;
 +
line-height:40px;
 +
z-index:1;
 +
background-color:#000;
 +
color:#fff;
 +
font-family:"微软雅黑","yahei",calibri, arial, helvetica, sans-serif,Italic;
 +
font-weight: bold;
 +
font-style: sans-serif;
 +
opacity:0.5;
 +
-moz-opacity:0.5;
 +
-webkit-opacity:0.5;
 +
filter:alpha(opacity=50);
 +
}
 +
.titleBox p span{
 +
opacity:1;
 +
-moz-opacity:1;
 +
-webkit-opacity:1;
 +
filter:alpha(opacity=100);
 +
}
 +
.titleBox p.active{
 +
bottom:0px;
 +
}
 +
/*-------------图标容器---------------*/
 +
.icoBox{
 +
position:absolute;  /*--固定定位--*/
 +
bottom:14px;
 +
right:15px;
 +
width:76px;
 +
height:12px;
 +
text-align:center;
 +
line-height:40px;
 +
z-index:2;
 +
}
 +
.icoBox span{
 +
display:block;
 +
float:left;
 +
height:12px;
 +
width:12px;
 +
margin-left:3px;
 +
overflow:hidden;
 +
background:url("https://static.igem.org/mediawiki/2014/a/a2/Ico.png") 0px 0px no-repeat;
 +
cursor:pointer;
 +
}
 +
.icoBox span.active {
 +
background-position:0px -12px;
 +
cursor:default;
 +
}
 +
 +
#content {
 +
    position: relative;
 +
    width:  1215px;
 +
#    margin-left: 10px;
 +
margin: 0 auto;
 +
    padding: 5px 5px 5px 5px;;
 +
    background: white;
 +
    color: black;
 +
    border-left: 1px solid #444444;
 +
    border-right: 1px solid #444444;
 +
    line-height: 1.5em;
 +
    z-index: 2;
 +
}
        
        
Line 47: Line 149:
#header{
#header{
height: 280px;
height: 280px;
-
background-image:url("https://static.igem.org/mediawiki/2014/a/a3/Newbanner.jpg");
+
background-image:url("https://static.igem.org/mediawiki/2014/4/49/New_New_banner.jpg");/* yan change to plant-vs HCHO*/
background-position: top center;
background-position: top center;
background-repeat: no-repeat;
background-repeat: no-repeat;
Line 64: Line 166:
}
}
 +
@font-face {
@font-face {
  font-family: ubuntu;
  font-family: ubuntu;
Line 70: Line 173:
.top-menu-each{
.top-menu-each{
height: 63px;
height: 63px;
 +
font-family: helveticallt,"微软雅黑";
font-family: helveticallt,"微软雅黑";
color: white;
color: white;
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float: left;
float: left;
cursor: pointer;
cursor: pointer;
-
font-weight: bold;
+
font-weight: bold;
}
}
Line 86: Line 190:
.arrow{
.arrow{
float: left;
float: left;
-
background-image: url("http://fudanigem.phx-2.ifreeurl.com/img/arrow.png");
+
background-image: url("http://fudanigem.phx-2.ifreeurl.com/img/arrow.png");/*needn't change*/
line-height: 63px;
line-height: 63px;
width: 12px;
width: 12px;
Line 150: Line 254:
padding-left: 35px;
padding-left: 35px;
padding-right: 10px;
padding-right: 10px;
-
background-image: url('http://fudanigem.phx-2.ifreeurl.com/img/big-photo-hover.png');
+
background-image: url('http://fudanigem.phx-2.ifreeurl.com/img/big-photo-hover.png');/*no change*/
background-repeat: no-repeat;
background-repeat: no-repeat;
margin-top: -130px;
margin-top: -130px;
Line 168: Line 272:
font-size: 30px;
font-size: 30px;
line-height: 30px;
line-height: 30px;
-
font-family: helveticallt,"微软雅黑";
+
font-family: helveticalmd,"微软雅黑";
font-weight: bold;
font-weight: bold;
padding-top: 25px;
padding-top: 25px;
Line 210: Line 314:
height: 180px;
height: 180px;
-
background-color: #394264;
+
background-color:white;
border-top-left-radius:8px;
border-top-left-radius:8px;
border-top-right-radius:8px;
border-top-right-radius:8px;
-
margin-top: 30px;
+
margin-top: 20px;
}
}
#main-content{
#main-content{
Line 229: Line 333:
height: 60px;
height: 60px;
background-color: #394264;
background-color: #394264;
-
font-family: helveticallt,"微软雅黑";
+
font-family: ubuntu,"宋体";
font-size: 18px;
font-size: 18px;
line-height: 60px;
line-height: 60px;
Line 254: Line 358:
#middle-photo{
#middle-photo{
float: left;
float: left;
-
margin-bottom: 25px;
+
margin-bottom: 445px;
}
}
.middle-photo-each{
.middle-photo-each{
-
padding:0px 0px 0px 0px;
+
padding:9px 9px 9px 9px;
background-color: white;
background-color: white;
margin-bottom: 15px;
margin-bottom: 15px;
-
margin-left: 0px;
+
margin-left: 250px;
}
}
Line 277: Line 381:
color: black;
color: black;
font-size: 16px;
font-size: 16px;
-
font-family: helveticallt,"微软雅黑";
+
font-family: "微软雅黑";
line-height: 20px;
line-height: 20px;
}
}
Line 291: Line 395:
}
}
#top{
#top{
-
background-image:url('http://fudanigem.phx-2.ifreeurl.com/img/top.png');
+
background-image: url('https://static.igem.org/mediawiki/2014/8/89/Uestc_top2.png');
-
width: 57px;
+
width: 65px;
-
height: 57px;
+
height: 138px;
-
position: fixed;
+
position: fixed;
-
top: 80%;
+
top: 70%;
-
margin-left: 1105px;
+
margin-left: 1200px;
-
cursor: pointer;
+
cursor: pointer;
 +
z-index: 999;
 +
 
}
}
#logo{
#logo{
position: absolute;
position: absolute;
-
background-image: url("https://static.igem.org/mediawiki/2014/9/99/NewLogo.png");
+
width: 154px;
width: 154px;
height: 147px;
height: 147px;
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color:white;
color:white;
}
}
 +
#intro1{
 +
font-family: helveticalmd,"微软雅黑";
 +
font-size: 50px;
 +
margin-top: 20px;
 +
margin-left: 10px;
 +
color: white;
 +
}
 +
#intro2{
 +
font-family: helveticalmd,"微软雅黑";
 +
font-size: 23px;
 +
margin-left: 10px;
 +
margin-right: 10px;
 +
color:white;
 +
text-align:justify;
 +
text-justify:inter-ideograph;
 +
text-indent:2em;
 +
}
 +
#introduction{
 +
background-color:#1bbc9b
 +
}
 +
/*rocket-to-top*/
 +
*{list-style:none;border:0;}
-
#SensorEditingArea{position:absolute; left:0px; top:300px; height:6500px; width:1213px; background-color:#ffffff; padding:60px 0px 0px 0px;}
+
#rocket-to-top div {
 +
    left: 0;
 +
    margin: 0;
 +
    overflow: hidden;
 +
    padding: 0;
 +
    position: absolute;
 +
    top: 0;
 +
    width: 149px;
 +
}
 +
#rocket-to-top .level-2 {
 +
    background: url("https://static.igem.org/mediawiki/2014/a/a6/UestcUp.png") no-repeat scroll -149px 0 transparent;
 +
    display: none;
 +
    height: 250px;
 +
    opacity: 0;
 +
    z-index: 1;
 +
}
 +
#rocket-to-top .level-3 {
 +
    background: none repeat scroll 0 0 transparent;
 +
    cursor: pointer;
 +
    display: block;
 +
    height: 150px;
 +
    z-index: 2;
 +
}
 +
#rocket-to-top {
 +
    background: url("https://static.igem.org/mediawiki/2014/a/a6/UestcUp.png") no-repeat scroll 0 0 transparent;
 +
    cursor: default;
 +
    display: block;
 +
    height: 250px;
 +
    margin: -125px 0 0;
 +
    overflow: hidden;
 +
    padding: 0;
 +
    position: fixed;
 +
    right: 0;
 +
    top: 80%;
 +
    width: 149px;
 +
    z-index: 11;
 +
}
 +
/*rocket-to-top end*/
 +
#intro2 img{
 +
margin:0px 0px 0px -10px;
 +
}
 +
</style>
 +
<script type="text/javascript" src="chrome-extension://bfbmjmiodbnnpllbbbfblcplfjjepjdn/js/injected.js"></script><style>@-moz-keyframes nodeInserted{from{opacity:0.99;}to{opacity:1;}}@-webkit-keyframes nodeInserted{from{opacity:0.99;}to{opacity:1;}}@-o-keyframes nodeInserted{from{opacity:0.99;}to{opacity:1;}}@keyframes nodeInserted{from{opacity:0.99;}to{opacity:1;}}embed,object{animation-duration:.001s;-ms-animation-duration:.001s;-moz-animation-duration:.001s;-webkit-animation-duration:.001s;-o-animation-duration:.001s;animation-name:nodeInserted;-ms-animation-name:nodeInserted;-moz-animation-name:nodeInserted;-webkit-animation-name:nodeInserted;-o-animation-name:nodeInserted;}</style>
 +
</head>
 +
<body>
 +
<div id="total-wrap">
 +
<div id="header">
 +
<div id="emp">
 +
<div id='logo'><a href="https://igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/d/da/NewLogo2.png"></a></div>
 +
</div>
 +
<div id="top-menu">
 +
<div class="top-menu-each">
 +
<a href="https://2014.igem.org/Team:UESTC-China"><p>Home</p></a>
 +
</div>
 +
<div class="top-menu-each">
 +
<div class="arrow"></div>
 +
<p>Team</p>
 +
<div class="top-menu-sub">
 +
<ul>
-
.SensorEditingAreaClass p{position:relative;left:0px; width:1140px; font-size:23px; font-family: calibri, arial, helvetica, sans-serif;  }
+
<a href="https://2014.igem.org/Team:UESTC-China/Team"><li>Members</li></a>
-
.SensorEditingAreaClass a{color:#1f8a70;}
+
<a href="https://2014.igem.org/Team:UESTC-China/Attribution"><li>Attribution</li></a>
 +
<a href="https://igem.org/Team.cgi?year=2014&team_name=UESTC-China"><li>Team Profile</li></a>
 +
<a href="https://2014.igem.org/Team:UESTC-China/Notebook"><li>Notebook</li></a>
 +
</ul>
 +
</div>
 +
</div>
-
#SectionTitles1{
 
-
position:relative;
 
-
left:30px;
 
-
background-color:#1f8a70;
 
-
padding:0 10px;
 
-
height:40px;
 
-
line-height:40px;
 
-
font-size:24px; font-family: calibri, arial, helvetica, sans-serif; border-bottom:0px; color:#ffffff; font-weight: bold;font-style: Italic; text-align:center; width:1140px; }
 
-
#SectionTitles2{
 
-
position:relative;
 
-
top:350px;
 
-
left:30px;
 
-
background-color:#1f8a70;
 
-
padding:0 10px;
 
-
height:40px;
 
-
line-height:40px;
 
-
font-size:24px; font-family: calibri, arial, helvetica, sans-serif; border-bottom:0px; color:#ffffff; font-weight: bold;font-style: Italic; text-align:center; width:1140px; }
 
-
#SectionTitles3{
+
<div class="top-menu-each">
-
position:relative;
+
<div class="arrow"></div>
-
left:30px;
+
<p>Project</p>
-
top: 720px;
+
<div class="top-menu-sub">
-
background-color:#1f8a70;
+
<ul>
-
padding:0 10px;
+
<a href="https://2014.igem.org/Team:UESTC-China/Project"><li>Overview</li></a>
-
height:40px;
+
<a href="https://2014.igem.org/Team:UESTC-China/Design"><li>Design</li></a>
-
line-height:40px;
+
<a href="https://2014.igem.org/Team:UESTC-China/result"><li>Result</li></a>
-
font-size:24px; font-family: calibri, arial, helvetica, sans-serif; border-bottom:0px; color:#ffffff; font-weight: bold;font-style: Italic; text-align:center; width:1140px; }
+
<a href="https://2014.igem.org/Team:UESTC-China/Futurework"><li>Future work</li></a>
 +
<a href="https://2014.igem.org/Team:UESTC-China/Judingform"><li>Judging Criteria</li></a>
-
#SectionTitles4{
+
</ul>
-
position:relative;
+
</div>
-
top: 1100px;
+
</div>
-
left:30px;
+
<div class="top-menu-each">
-
background-color:#1f8a70;
+
<div class="arrow"></div>
-
padding:0 10px;
+
<p>Modeling</p>
-
height:40px;
+
<div class="top-menu-sub">
-
line-height:40px;
+
<ul>
-
font-size:24px; font-family: calibri, arial, helvetica, sans-serif; border-bottom:0px; color:#ffffff; font-weight: bold;font-style: Italic; text-align:center; width:1140px; }
+
<a href="https://2014.igem.org/Team:UESTC-China/Modeling"><li>Overview</li></a>
 +
<a href="https://2014.igem.org/Team:UESTC-China/Modeling1"><li>HPS/PHI</li></a>
 +
<a href="https://2014.igem.org/Team:UESTC-China/Modeling2"><li>FALDH/FDH</li></a>
 +
<a href="https://2014.igem.org/Team:UESTC-China/Modeling3"><li>AtAHA2</a>
 +
</ul>
 +
</div>
 +
</div>
 +
<div class="top-menu-each">
 +
<div class="arrow"></div>
 +
<p>Parts</p>
 +
<div class="top-menu-sub">
 +
<ul>
 +
<a href="https://2014.igem.org/Team:UESTC-China/BioBrick"><li>BioBrick</li></a>
 +
<a href="https://2014.igem.org/Team:UESTC-China/Protocol"><li>Protocol</li></a>
-
#SectionTitles5{
+
</ul>
-
position:relative;
+
</div>
-
top: 1500px;
+
</div>
-
left:30px;
+
<div class="top-menu-each">
-
padding:0 10px;
+
<div class="arrow"></div>
-
height:40px;
+
<p>Human Practice</p>
-
line-height:40px;
+
<div class="top-menu-sub">
-
font-size:24px; font-family: calibri, arial, helvetica, sans-serif; border-bottom:0px; color:#ffffff; font-weight: bold;font-style: Italic; text-align:center; width:1140px; }
+
<ul>
 +
<a href="https://2014.igem.org/Team:UESTC-China/Questionnaire.html"><li>Questionnaire</li></a>
-
#SectionTitles6{
+
<a href="https://2014.igem.org/Team:UESTC-China/Knowledgequiz"><li>Knowledge quiz</li></a>
-
position:relative;
+
<a href="https://2014.igem.org/Team:UESTC-China/Lecture.html"><li>Lecture</li></a>
-
top: 1850px;
+
<a href="https://2014.igem.org/Team:UESTC-China/Communication.html"><li>Communication</li></a>
-
left:30px;
+
<a href="https://2014.igem.org/Team:UESTC-China/Art"><li>Art</li></a>
-
background-color:#1f8a70;
+
</ul>
-
padding:0 10px;
+
</div>
-
height:40px;
+
</div>
-
line-height:40px;
+
 
-
font-size:24px; font-family: calibri, arial, helvetica, sans-serif; border-bottom:0px; color:#ffffff; font-weight: bold;font-style: Italic; text-align:center; width:1140px; }
+
<div class="top-menu-each">
 +
<div class="arrow"></div>
 +
<p>Safety</p>
 +
<div class="top-menu-sub">
 +
<ul>
 +
<a href="https://2014.igem.org/Team:UESTC-China/Safety"><li>Safety</li></a>
 +
</ul>
 +
</div>
 +
</div>
-
#SectionTitles7{
 
-
top: 2290px;
 
-
position:relative;
 
-
left:30px;
 
-
padding:0 10px;
 
-
height:40px;
 
-
line-height:40px;
 
-
font-size:24px; font-family: calibri, arial, helvetica, sans-serif; border-bottom:0px; color:#ffffff; font-weight: bold;font-style: Italic; text-align:center; width:1140px; }
 
-
#SectionTitles8{
 
-
top: 2650px;
 
-
position:relative;
 
-
left:30px;
 
-
background-color:#1f8a70;
 
-
padding:0 10px;
 
-
height:40px;
 
-
line-height:40px;
 
-
font-size:24px; font-family: calibri, arial, helvetica, sans-serif; border-bottom:0px; color:#ffffff; font-weight: bold;font-style: Italic; text-align:center; width:1140px; }
 
-
#SectionTitles9{
 
-
top: 3000px;
 
-
position:relative;
 
-
left:30px;
 
-
padding:0 10px;
 
-
height:40px;
 
-
line-height:40px;
 
-
font-size:24px; font-family: calibri, arial, helvetica, sans-serif; border-bottom:0px; color:#ffffff; font-weight: bold;font-style: Italic; text-align:center; width:1140px; }
 
-
.textEditingArea{
 
-
top:3800px;
 
-
position:absolute;
 
-
}
 
-
.textEditingTitle{
 
-
position:relative;
 
-
margin-right:20px;
 
-
background-color:#1f8a70;
 
-
left:30px;
 
-
padding:0 10px;
 
-
height:40px;
 
-
line-height:40px;
 
-
font-size:24px; font-family: calibri, arial, helvetica, sans-serif; border-bottom:0px;
 
-
color: #ffffff;text-align:center; width:1100px;
 
-
}
 
-
.textEditingstyle{
 
-
position:fixed;
 
-
color: #000000;line-height:40px;
 
-
font-size:24px; font-family:Microsoft Yahei; border-bottom:0px;
 
-
text-align:left; width:1140px; }
 
-
 
-
#BiosensorsTable{position:absolute;margin-top: 20px; left:0px; width:1000px; font-family:calibri;arial;helvetica;sans-serif; font-size:18px; text-align:center; }
 
-
.mid{background: #FFFFCC;color: #797268; }
 
-
.top{background: #FFFF00; color: #797268; }
 
-
</style>
 
-
</head>
 
-
<body>
 
-
<div id="total-wrap">
 
-
<div id="header">
 
-
  <div id="emp">
 
-
    <div id='logo'><a href="https://igem.org"></a></div>
 
-
  </div>
 
-
  <div id="top-menu">
 
-
    <div class="top-menu-each"> <a href="https://2014.igem.org/Team:UESTC-China">
 
-
      <p>Home</p>
 
-
      </a> </div>
 
-
    <div class="top-menu-each">
 
-
      <div class="arrow"></div>
 
-
      <p>Team</p>
 
-
      <div class="top-menu-sub">
 
-
        <ul>
 
-
          <a href="https://2014.igem.org/Team:UESTC-China/Team">
 
-
          <li>Members</li>
 
-
          </a> <a href="https://2014.igem.org/Team:UESTC-China/Attribution">
 
-
          <li>Attribution</li>
 
-
          </a> <a href="https://igem.org/Team.cgi?year=2014&team_name=UESTC-China">
 
-
          <li>Team Profile</li>
 
-
          </a> <a href="https://2014.igem.org/Team:UESTC-China/Notebook">
 
-
          <li>Notebook</li>
 
-
          </a>
 
-
        </ul>
 
-
      </div>
 
-
    </div>
 
-
    <div class="top-menu-each">
 
-
      <div class="arrow"></div>
 
-
      <p>Project</p>
 
-
      <div class="top-menu-sub">
 
-
        <ul>
 
-
          <a href="https://2014.igem.org/Team:UESTC-China/Project">
 
-
          <li>Overview</li>
 
-
          </a> <a href="https://2014.igem.org/Team:UESTC-China/Design">
 
-
          <li>Design</li>
 
-
          </a> <a href="https://2014.igem.org/Team:UESTC-China/result">
 
-
          <li>Result</li>
 
-
          </a> <a href="https://2014.igem.org/Team:UESTC-China/Futurework">
 
-
          <li>Future work</li>
 
-
          </a>
 
-
        </ul>
 
-
      </div>
 
-
    </div>
 
-
    <div class="top-menu-each">
 
-
      <div class="arrow"></div>
 
-
      <p>Modeling</p>
 
-
      <div class="top-menu-sub">
 
-
        <ul>
 
-
          <a href="https://2014.igem.org/Team:UESTC-China/Modeling">
 
-
          <li>Modeling</li>
 
-
          </a>
 
-
        </ul>
 
-
      </div>
 
-
    </div>
 
-
    <div class="top-menu-each">
 
-
      <div class="arrow"></div>
 
-
      <p>Parts</p>
 
-
      <div class="top-menu-sub">
 
-
        <ul>
 
-
          <a href="https://2014.igem.org/Team:UESTC-China/Material">
 
-
          <li>BioBrick</li>
 
-
          </a> <a href="https://2014.igem.org/Team:UESTC-China/Protocol">
 
-
          <li>Protocol</li>
 
-
          </a>
 
-
        </ul>
 
-
      </div>
 
-
    </div>
 
-
    <div class="top-menu-each">
 
-
      <div class="arrow"></div>
 
-
      <p>Human Practice</p>
 
-
      <div class="top-menu-sub">
 
-
        <ul>
 
-
          <a href="https://2014.igem.org/Team:UESTC-China/HumanPractice.html">
 
-
          <li>Knowledge quiz</li>
 
-
          </a> <a href="https://2014.igem.org/Team:UESTC-China/Lecture.html">
 
-
          <li>Lecture</li>
 
-
          </a> <a href="https://2014.igem.org/Team:UESTC-China/Communication.html">
 
-
          <li>Communication</li>
 
-
          </a> <a href="https://2014.igem.org/Team:UESTC-China/Questionnaire.html">
 
-
          <li>Questionnaire</li>
 
-
          </a>
 
-
        </ul>
 
-
      </div>
 
-
    </div>
 
-
    <div class="top-menu-each">
 
-
      <div class="arrow"></div>
 
-
      <p>Safety</p>
 
-
      <div class="top-menu-sub">
 
-
        <ul>
 
-
          <a href="https://2014.igem.org/Team:UESTC-China/Safety">
 
-
          <li>Safety</li>
 
-
          </a>
 
-
        </ul>
 
-
      </div>
 
-
    </div>
 
-
  </div>
 
</div>
</div>
-
<div id="middle-photo">
+
</div>
-
  <div id="SensorEditingArea" class="SensorEditingAreaClass">
+
<div class="imageRotation">
-
  <div class="tableEditing">
+
<div class="imageBox" style="width: 1214px; left: 0px;">
-
    <div id="SectionTitles1" style="width:500px;">DNA ligation with T4 DNA Ligase
+
<div><img src="https://static.igem.org/mediawiki/2014/2/21/All_students1.jpg"></div>
-
      </h1>
+
<div><img src="https://static.igem.org/mediawiki/2014/1/15/NewPlant1.jpg"></div>
-
      <table border="1" id="BiosensorsTable" cellspacing="0";>
+
<div><img src="https://static.igem.org/mediawiki/2014/8/83/Home1.jpg"></div>
-
        <tr>
+
<div><img src="https://static.igem.org/mediawiki/2014/8/8b/Xiaojiejie.jpg"></div>
-
          <th class="top">Component</th>
+
                <div><img src="https://static.igem.org/mediawiki/2014/c/ce/Humanpractice.jpg"></div>
-
          <th class="top">20ul Reaction</th>
+
         <!--<div><img src="https://static.igem.org/mediawiki/2014/1/14/All_students3.jpg"></div>-->
-
        </tr>
+
</div>
-
        <tr>
+
    <div class="titleBox">
-
          <td class="mid">1.33X Master Mix</td>
+
    <p class="active" style="bottom: 0px;"><span>Our Team: Brilliant Guys</span></p>
-
          <td class="mid"> 15ul</td>
+
         <p style="bottom: -40px;">Plants: Transgenic Tobaccos</p>
-
        </tr>
+
         <p style="bottom: -40px;">Super Plant: Plants vs HCOH</p>
-
        <tr>
+
         <p style="bottom: -40px;">Safety: Operation</p>
-
          <td class="mid">Insert DNA </td>
+
         <p style="bottom: -40px;">Human Practice</p>
-
          <td class="mid"> the moles of insert DNA to vector DNA is 5:1</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">>Vector DNA</td>
+
-
          <td class="mid"> 50ng</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">ddH2O</td>
+
-
          <td class="mid"> To 20ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Incubate at 50℃ for 1 hour</td>
+
-
          <td class="mid"></td>
+
-
        </tr>
+
-
      </table>
+
-
    </div>
+
-
    <div id="SectionTitles2" style="width:500px;">DNA ligation with Gibson Assemble
+
-
      </h1>
+
-
      <table border="1" id="BiosensorsTable" cellspacing="0";>
+
-
        <tr>
+
-
          <th class="top">Component</th>
+
-
          <th class="top">20ul Reaction </th>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Vector DNA</td>
+
-
          <td class="mid">50ng</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Insert DNA</td>
+
-
          <td class="mid"> the moles of insert DNA to vector DNA is 5:1</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10X T4 DNA ligase buffer</td>
+
-
          <td class="mid">2ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">T4 DNA ligase</td>
+
-
          <td class="mid">1ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">ddH2O</td>
+
-
          <td class="mid">to 20ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Incubate at 25℃ for 12 hours</td>
+
-
          <td class="mid"></td>
+
-
        </tr>
+
-
      </table>
+
-
    </div>
+
-
    <div id="SectionTitles3" style="width:500px;">Double Digestion of DNA
+
-
      </h1>
+
-
      <table border="1" id="BiosensorsTable" cellspacing="0";>
+
-
        <tr>
+
-
          <th class="top">Component</th>
+
-
          <th class="top">50ul Reaction </th>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10x FastDigest buffer</td>
+
-
          <td class="mid">5ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Restriction enzyme 1</td>
+
-
          <td class="mid"> 1ul</td>
+
-
         </tr>
+
-
        <tr>
+
-
          <td class="mid">Restriction enzyme 2</td>
+
-
          <td class="mid">1ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">DNA</td>
+
-
          <td class="mid">1-2ug</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">ddH2O</td>
+
-
          <td class="mid">to 50ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Incubate at 37℃ for 2 hours</td>
+
-
          <td class="mid"></td>
+
-
        </tr>
+
-
      </table>
+
-
    </div>
+
-
    <div id="SectionTitles4" style="width:500px;">PCR protocol for KOD-Plus-Neo
+
-
      </h1>
+
-
      <table border="1" id="BiosensorsTable" cellspacing="0";>
+
-
        <tr>
+
-
          <th class="top">Component</th>
+
-
          <th class="top">50ul Reaction </th>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">ddH2O</td>
+
-
          <td class="mid">33ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10X buffer for KOD-Plus-Neo</td>
+
-
          <td class="mid"> 5ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">MgSO4</td>
+
-
          <td class="mid">1ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10mM dNTPs</td>
+
-
          <td class="mid">1ug</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10uM forward primer</td>
+
-
          <td class="mid">1ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10uM reverse primer</td>
+
-
          <td class="mid"> 1ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Template</td>
+
-
          <td class="mid">1ul </td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">KOD enzyme</td>
+
-
          <td class="mid"> 1ul</td>
+
-
        </tr>
+
-
      </table>
+
-
    </div>
+
-
    <div id="SectionTitles5" style="width:500px;">
+
-
      <table border="1" id="BiosensorsTable" cellspacing="0";>
+
-
        <tr>
+
-
          <th class="top"></th>
+
-
          <th class="top">Temperature </th>
+
-
          <th class="top">Time </th>
+
-
          <th class="top"> Cycle</th>
+
-
         </tr>
+
-
        <tr>
+
-
          <td class="mid">Step1</td>
+
-
          <td class="mid">94℃</td>
+
-
          <td class="mid">30s</td>
+
-
          <td class="mid">X35 cycles</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid"></td>
+
-
          <td class="mid"> 56℃</td>
+
-
          <td class="mid">30s</td>
+
-
          <td class="mid"></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid"></td>
+
-
          <td class="mid">68℃</td>
+
-
          <td class="mid">30s/kb</td>
+
-
          <td class="mid"></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Step3</td>
+
-
          <td class="mid">68℃</td>
+
-
          <td class="mid">5min</td>
+
-
          <td class="mid">X1 cycles</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid"></td>
+
-
          <td class="mid">10℃</td>
+
-
          <td class="mid">10min</td>
+
-
          <td class="mid"></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10uM reverse primer</td>
+
-
          <td class="mid"> 1ul</td>
+
-
          <td class="mid">ddH2O</td>
+
-
          <td class="mid">33ul</td>
+
-
        </tr>
+
-
      </table>
+
-
    </div>
+
-
    <div id="SectionTitles6" style="width:500px;">PCR protocol for KOD-Plus-Neo
+
-
      </h1>
+
-
      <table border="1" id="BiosensorsTable" cellspacing="0";>
+
-
        <tr>
+
-
          <th class="top">Component</th>
+
-
          <th class="top">50ul Reaction </th>
+
-
         </tr>
+
-
        <tr>
+
-
          <td class="mid">ddH2O</td>
+
-
          <td class="mid">32ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10X buffer for KOD-Plus-Neo</td>
+
-
          <td class="mid"> 5ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">MgSO4</td>
+
-
          <td class="mid">3ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10mM dNTPs</td>
+
-
          <td class="mid">5ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10uM forward primer</td>
+
-
          <td class="mid">1ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10uM reverse primer</td>
+
-
          <td class="mid"> 1ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Template</td>
+
-
          <td class="mid">1ul </td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">KOD enzyme</td>
+
-
          <td class="mid"> 1ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Each template will be diluted by the number of moles into 2-10ng/ul.</td>
+
-
          <td class="mid"></td>
+
-
        </tr>
+
-
      </table>
+
-
    </div>
+
-
    <div id="SectionTitles7" style="width:500px;">
+
-
      <table border="1" id="BiosensorsTable" cellspacing="0";>
+
-
        <tr>
+
-
          <th class="top"></th>
+
-
          <th class="top">Temperature </th>
+
-
          <th class="top">Time </th>
+
-
          <th class="top"> Cycle</th>
+
-
         </tr>
+
-
        <tr>
+
-
          <td class="mid">Step1</td>
+
-
          <td class="mid">94℃</td>
+
-
          <td class="mid">5min</td>
+
-
          <td class="mid">X1 cycle</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Step2</td>
+
-
          <td class="mid"> 94℃</td>
+
-
          <td class="mid">30s</td>
+
-
          <td class="mid">X35 cycles</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid"></td>
+
-
          <td class="mid">56℃</td>
+
-
          <td class="mid">30s</td>
+
-
          <td class="mid"></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid"></td>
+
-
          <td class="mid">68℃</td>
+
-
          <td class="mid">30s/kb</td>
+
-
          <td class="mid"></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Step3</td>
+
-
          <td class="mid"> 68℃</td>
+
-
          <td class="mid">5min</td>
+
-
          <td class="mid">X1 cycles</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid"></td>
+
-
          <td class="mid"> 10℃</td>
+
-
          <td class="mid">10min</td>
+
-
          <td class="mid"></td>
+
-
        </tr>
+
-
      </table>
+
-
    </div>
+
-
    <div id="SectionTitles8" style="width:500px;">Colony PCR with Taq DNA polymerase
+
-
      </h1>
+
-
      <table border="1" id="BiosensorsTable" cellspacing="0";>
+
-
         <tr>
+
-
          <th class="top">Component</th>
+
-
          <th class="top">25ul Reaction </th>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">ddH2O</td>
+
-
          <td class="mid">15.8ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10X Taq buffer</td>
+
-
          <td class="mid"> 2.5ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">2.mM dNTPs</td>
+
-
          <td class="mid">0.5ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10uM forward primer</td>
+
-
          <td class="mid">0.5ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">10uM reverse primer</td>
+
-
          <td class="mid">0.5ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Taq enzyme</td>
+
-
          <td class="mid"> 0.2ul</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Water with colony </td>
+
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          <td class="mid">5ul </td>
+
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        </tr>
+
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      </table>
+
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    </div>
+
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    <div id="SectionTitles9" style="width:500px;">
+
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      <table border="1" id="BiosensorsTable" cellspacing="0";>
+
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        <tr>
+
-
          <th class="top"></th>
+
-
          <th class="top">Temperature </th>
+
-
          <th class="top">Time </th>
+
-
          <th class="top"> Cycle</th>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Step1</td>
+
-
          <td class="mid">95℃</td>
+
-
          <td class="mid">5min</td>
+
-
          <td class="mid">X1 cycles</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Step2</td>
+
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          <td class="mid"> 94℃</td>
+
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          <td class="mid">30s</td>
+
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          <td class="mid">X35 cycles</td>
+
-
        </tr>
+
-
        <tr>
+
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          <td class="mid"></td>
+
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          <td class="mid">56℃</td>
+
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          <td class="mid">30s</td>
+
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          <td class="mid"></td>
+
-
        </tr>
+
-
        <tr>
+
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          <td class="mid"></td>
+
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          <td class="mid">72℃</td>
+
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          <td class="mid">1min/kb</td>
+
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          <td class="mid"></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid">Step3</td>
+
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          <td class="mid">72℃</td>
+
-
          <td class="mid">10min</td>
+
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          <td class="mid">X1 cycles</td>
+
-
        </tr>
+
-
        <tr>
+
-
          <td class="mid"></td>
+
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          <td class="mid">10℃</td>
+
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          <td class="mid">10min</td>
+
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          <td class="mid"></td>
+
-
        </tr>
+
-
      </table>
+
     </div>
     </div>
 +
<div class="icoBox">
 +
<span class="active" rel="1">1</span>
 +
<span rel="2" class="">2</span>
 +
<span rel="3" class="">3</span>
 +
<span rel="4" class="">4</span>
 +
        <span rel="5" class="">5</span>
</div>
</div>
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  <div class="textEditingArea" ><div class="textEditingTitle" style="width: 800px">E. coli Calcium Chloride competent cell protocol</br>
+
</div>
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  <p class="textEditingstyle">1)Streak E.coli cells (DH5a) on an LB plate; (BL21(DE3)LysS cells on LB plate+34 mg/ml chloramphenicol)<br>
+
<div id="introduction">
-
2) Allow cells to grow at 37℃ overnight<br>
+
<p id="intro1"><b>Introduction</b></p>
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3)Place one colony in 10 ml LB media (+antibiotic selection if necessary), grow overnight at 37℃<br>
+
<br/>
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4) Take 2 ml LB media and save for blank. Transfer 5 ml overnight DH5a culture into 500 ml LB media in 3 L flask<br>
+
<p id="intro2" style="color:white;text-indent: 0em">
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5) Allow cell to grow at 37℃ (250 rpm), until OD600= 0.4 (~2-3 hours)<br>
+
With the rapid society development, a large amount of pollutants are full of our daily life. Nowadays, chemical materials are widely used in our surroundings indoor, such as building materials, paint in new apartments, artificial adhesives in numerous household products and etc, which may lead to excess release of formaldehyde in closed space. Exposing to the substantial formaldehyde may cause irritation, allergic asthma and neurasthenia, as well as carcinogenesis. Nowadays, people mainly get rid of the formaldehyde by increasing the rate of ventilating or using air purifier in those polluted spaces. However, these methods show some limitation, and cannot degrade formaldehyde safely and effectively.
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6) Transfer cells to 2 centrifuge bottles (250 ml), and place cells on ice for 20 min<br>
+
<br/><br/>
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7) Centrifuge cells in at 4oC for 10 min at 3,000 g and subsequent resuspension may be done in the same bottle. Cells must remain cold for the rest of the procedure: Transport tubes on ice and resuspend on ice in the cold room<br>
+
To get the inspiration for the design of our project, we did a survey of the knowledge concerning formaldehyde pollution. The results showed that people paid high attention to the indoor formaldehyde pollution, and preferred to adopt a safe and environmental friendly method to decrease the concentration of formaldehyde effectively. A methodology by using green plants got the highest score among all provided approaches. Based on the results of this survey, we decided to use synthetic biology strategy to design a super plant that can remove formaldehyde safely and effectively. 
-
8) Pour off media and resuspend cells in 30 ml of cold 0.1 M CaCl2. Transfer the suspended cells into 50 ml polypropylene tubes, and incubate on ice for 30 min<br>
+
<br/><br/>
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9) Centrifuge cells at 4O℃ for 10 min at 3,000 g<br>
+
After doing sufficient literature research, we got to know that there is a formaldehyde-fixation pathway in methylotrophs which is called ribulose monophosphate (RuMP) pathway. HPS and PHI are the two key enzymes in this pathway. Besides, there is a formaldehyde folate-independent pathway in some certain plant species. FALDH and FDH play an important role in this pathway. In our 2014 iGEM project, these two pathways were integrated through the methodology of synthetic biology. For this, formaldehyde metabolism related genes from both methylotrophs and plants were transferred into tobacco to rebuild a new formaldehyde metabolism pathway in our super plant.
-
10) Pour supernatant and resuspend cells (by pipetting) in 8 ml cold 0.1M CaCl2 containing 15% glycerol. Transfer 140 ml into (1.5 ml) Ependorff tubes placed on ice. Freeze the cells in liquid nitrogen. Cells stored at -80oC can be used for transformation for up to ~6 months<br>
+
<br/><br/>
-
11) Add 10 to 40 ng (10 to 25 ml volume) of DNA to 250 ml of competent cells in step<br>
+
To further increase the degradation efficiency of formaldehyde, we co-expressed gene <i>AHA2</i>, which is original from <i>Arabidopsis thaliana</i>, to regulate stomata opening. In addition, by taking into account of biosafety of genetically modified organism, we introduced the gene of cysteine protease into our super plants. The transgenic tobacco pollen will be aborted while cysteine protease gene is specifically expressed in tobacco tapetum.
-
12) Incubate the mixture on ice for 30 minutes.<br>
+
<br/><br/>
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13) Transfer the reaction to a 42℃ water for 1min.<br>
+
With more and more achievements have been harvested about the super plant, we organized various activities such as questionnaire survey, school seminar series, video making, etc, to vigorously promote synthetic biology, iGEM and our super plant. Along with the happy summer vacation, we had completed our project successfully with the efforts of all members. Let's meet at MIT and share each other's achievenments.
-
14) Add 0.9 ml of LB culture to each tube and incubate at 37℃ for 1 hour in a roller drum (250 rpm) to allow cells to recover and express the antibiotic resistance marker. <br>
+
<br/><br/>
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15) Incubate on ice for 2 minutes. <br>
+
 
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16) Spread the appropriate quantity of cells (50 to 100 ml) on selective media. Store the remaining cells at 4℃. <br>
+
<img  style="height:300px;width:1215px" src="https://static.igem.org/mediawiki/2014/8/89/Jiantu5.jpg" alt="pic">
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(A) E. coli cells from the control tube without DNA in step 12 above are plated on selective medium and nonselective medium. The first plating ensures that the selective medium is working properly since no growth should be observed. The second plating provides the number of viable cells in the absence of selective medium. <br>
+
-
(B) E. coli cells being tested for competency are plated on LB agar containing ampicillin (50 mg/ml final concentration) to ensure that the transformation efficiency has not decreased over time due to storage.<br>
+
-
17) Incubate all plates overnight at 37℃ (agar side up). <br>
+
-
18) Count the number of colonies.<br>
+
</p>
</p>
</div>
</div>
-
  </div>
 
-
 
+
<div id="footer">
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+
 
 +
<img  src='https://static.igem.org/mediawiki/2014/3/32/Foot6.jpg' />
 +
 
 +
 
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</div>
 +
</div>
 +
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Latest revision as of 15:14, 17 October 2014

Plant Vs HCHO

Our Team: Brilliant Guys

Plants: Transgenic Tobaccos

Super Plant: Plants vs HCOH

Safety: Operation

Human Practice

1 2 3 4 5

Introduction


With the rapid society development, a large amount of pollutants are full of our daily life. Nowadays, chemical materials are widely used in our surroundings indoor, such as building materials, paint in new apartments, artificial adhesives in numerous household products and etc, which may lead to excess release of formaldehyde in closed space. Exposing to the substantial formaldehyde may cause irritation, allergic asthma and neurasthenia, as well as carcinogenesis. Nowadays, people mainly get rid of the formaldehyde by increasing the rate of ventilating or using air purifier in those polluted spaces. However, these methods show some limitation, and cannot degrade formaldehyde safely and effectively.

To get the inspiration for the design of our project, we did a survey of the knowledge concerning formaldehyde pollution. The results showed that people paid high attention to the indoor formaldehyde pollution, and preferred to adopt a safe and environmental friendly method to decrease the concentration of formaldehyde effectively. A methodology by using green plants got the highest score among all provided approaches. Based on the results of this survey, we decided to use synthetic biology strategy to design a super plant that can remove formaldehyde safely and effectively.

After doing sufficient literature research, we got to know that there is a formaldehyde-fixation pathway in methylotrophs which is called ribulose monophosphate (RuMP) pathway. HPS and PHI are the two key enzymes in this pathway. Besides, there is a formaldehyde folate-independent pathway in some certain plant species. FALDH and FDH play an important role in this pathway. In our 2014 iGEM project, these two pathways were integrated through the methodology of synthetic biology. For this, formaldehyde metabolism related genes from both methylotrophs and plants were transferred into tobacco to rebuild a new formaldehyde metabolism pathway in our super plant.

To further increase the degradation efficiency of formaldehyde, we co-expressed gene AHA2, which is original from Arabidopsis thaliana, to regulate stomata opening. In addition, by taking into account of biosafety of genetically modified organism, we introduced the gene of cysteine protease into our super plants. The transgenic tobacco pollen will be aborted while cysteine protease gene is specifically expressed in tobacco tapetum.

With more and more achievements have been harvested about the super plant, we organized various activities such as questionnaire survey, school seminar series, video making, etc, to vigorously promote synthetic biology, iGEM and our super plant. Along with the happy summer vacation, we had completed our project successfully with the efforts of all members. Let's meet at MIT and share each other's achievenments.

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