Team:Bielefeld-CeBiTec

From 2014.igem.org

(Difference between revisions)
 
(50 intermediate revisions not shown)
Line 1: Line 1:
-
{{Template:Team:Bielefeld-CeBiTec/CSS/main.css}}
+
{{Template:Team:Bielefeld-CeBiTec/igem.css}}
-
{{Template:Team:Bielefeld-CeBiTec/Header}}
+
{{Template:Team:Bielefeld-CeBiTec/main.css}}
 +
{{Template:Team:Bielefeld-CeBiTec/basic.tmpl}}
<html>
<html>
-
 
-
<style>
 
-
td {
 
-
    font-size:18px
 
-
}
 
-
</style>
 
-
 
-
<table width="70%" align="center">
 
-
 
-
<!--navigation menu -->
 
-
<td align="center" colspan="3">
 
-
 
-
<table  width="950px">
 
-
<tr heigth="15px"></tr>
 
-
<tr heigth="75px">
 
<!-- automatisches Setzen des Projektüberblicks-->
<!-- automatisches Setzen des Projektüberblicks-->
<style>
<style>
#project_overview{
#project_overview{
 +
  float:left;
}
}
<!--relative Angabe für die Überlappenden Module-->
<!--relative Angabe für die Überlappenden Module-->
-
#module_eins{
+
#moduleX{
-
position:relative;
+
-
top:-400px;
+
-
left:-350px;
+
}
}
 +
 +
#module1{
 +
  float:left;
 +
  position:relative;
 +
  left:-170px;
 +
}
 +
#fullpicture{
#fullpicture{
-
float:left;
+
    margin-left:auto;
 +
    margin-right:auto;
 +
    width: 932px;
 +
    padding:0px;
 +
    height:400px;
 +
}
 +
#fullpicture1{
 +
    background-image: url(https://static.igem.org/mediawiki/2014/6/61/Bielefeld-CeBiTec_14-06-08_Project_Module_1.png);
 +
    background-size: 100% 100%;
 +
    float: left;
 +
    height:400px;
 +
    width:446px;
 +
}
 +
#fullpicture1:hover{
 +
    background-image:url(https://static.igem.org/mediawiki/2014/d/d3/Bielefeld-CeBiTec_2014-06-04_Modul1.png);
 +
}
 +
#fullpicture2{
 +
    background-image: url(https://static.igem.org/mediawiki/2014/f/f6/Bielefeld-CeBiTec_14-06-08_Project_Space_1.png);
 +
    background-size: 100% 100%;
 +
    float: left;
 +
    margin-left: -85px;
 +
    height:400px;
 +
    width:158px;   
 +
}
 +
#fullpicture3{
 +
    background-image: url(https://static.igem.org/mediawiki/2014/0/03/Bielefeld-CeBiTec_14-06-08_Project_Module_2.png);
 +
    background-size:100% 100%;
 +
    float: left;
 +
    margin-left: -85px;
 +
    height:400px;
 +
    width:330px;
 +
}
 +
#fullpicture3:hover{
 +
    background-image:url(https://static.igem.org/mediawiki/2014/b/bb/Bielefeld-CeBiTec_2014-06-07_Modul2.png);
 +
}
 +
#fullpicture4{
 +
    background-image: url(https://static.igem.org/mediawiki/2014/9/9c/Bielefeld-CeBiTec_14-06-08_Project_Space_2.png);
 +
    background-size:100% 100%;
 +
    float: left;
 +
    margin-left: -97px;
 +
    height:400px;
 +
    width:196px;
 +
}
 +
#fullpicture5{
 +
    background-image: url(https://static.igem.org/mediawiki/2014/b/b3/Bielefeld-CeBiTec_14-06-08_Project_Module_3.png);
 +
    background-size:100% 100%;
 +
    float: left;
 +
    margin-left: -140px;
 +
    height:400px;
 +
    width:209px;
 +
}
 +
#fullpicture5:hover{
 +
    background-image:url(https://static.igem.org/mediawiki/2014/7/75/Bielefeld-CeBiTec_2014-06-07_Modul3.png);
}
}
Line 38: Line 81:
<body>
<body>
-
<br>
 
-
<h1>Welcome to our project </h1>
 
-
<div id="fullpicture"><img src="https://static.igem.org/mediawiki/2014/6/61/Bielefeld-CeBiTec_14-06-08_Project_Module_1.png"></div>
+
<h1>Welcome to our project </h1>
-
<div id="fullpicture"><img src="https://static.igem.org/mediawiki/2014/f/f6/Bielefeld-CeBiTec_14-06-08_Project_Space_1.png"></div>
+
<table style="background-color:transparent;">
-
<div id="fullpicture"><img src="https://static.igem.org/mediawiki/2014/0/03/Bielefeld-CeBiTec_14-06-08_Project_Module_2.png"></div>
+
<tr>
-
<div id="fullpicture"><img src="https://static.igem.org/mediawiki/2014/9/9c/Bielefeld-CeBiTec_14-06-08_Project_Space_2.png"></div>
+
<td><!--modul 1-->
-
<div id="fullpicture"><img src="https://static.igem.org/mediawiki/2014/b/b3/Bielefeld-CeBiTec_14-06-08_Project_Module_3.png"></div>
+
<div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:155px">
 +
      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC" target="_blank"><img src="https://static.igem.org/mediawiki/2014/d/d2/Bielefeld-CeBiTec_14-10-16_Mediator_NeutralRed.png" width="155px"></a><br>
 +
      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC" target="_blank"><b>Results of module 1 - rMFC</b></a></font>
 +
    </div>
 +
</td>
 +
<td><!--modul 2-->
 +
<div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:155px">
 +
      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation" target="_blank"><img src="https://static.igem.org/mediawiki/2014/c/c7/Bielefeld-CeBiTec_2014-10-12_Carboxysom.png" width="155px"></a><br>
 +
      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation" target="_blank"><b>Results of module 2 - CO<sub>2</sub> fixation</b></a></font>
 +
    </div>
 +
</td>
 +
<td><!--modul 3-->
 +
<div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:155px">
 +
      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Isobutanol" target="_blank"><img src="https://static.igem.org/mediawiki/2014/1/11/Bielefeld-CeBiTec_14-10-17_IsobutProd_37.png" width="155px"></a><br>
 +
      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Isobutanol" target="_blank"><b>Results of module 3 - Isobutanol production</b></a></font>
 +
    </div>
 +
</td>
 +
<td><!--biosafety-->
 +
<div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:155px">
 +
      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Biosafety" target="_blank"><img src="https://static.igem.org/mediawiki/2014/7/7b/Bielefeld-CeBiTec_2014-10-13_Biosafety_Selection.png" width="155px"></a><br>
 +
      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Biosafety" target="_blank"><b>Results of our biosafety</b></a></font>
 +
    </div>
 +
</td>
 +
<td><!--synenergene-->
 +
<div class="element" style="margin:10px; padding:10px; text-align:center; float:left; width:155px">
 +
      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Synenergene" target="_blank"><img src="https://static.igem.org/mediawiki/2014/5/51/Bielefeld-CeBiTec_2014-08-31_Fig._4_-_Szen_Windkraft.png" width="155px"></a><br>
 +
      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Synenergene" target="_blank"><b>SYNENERGENE/ Applications</b></a></font>
 +
    </div>
 +
</td>
 +
</tr>
 +
</table>
 +
<div class="element">
 +
<div id="text">
 +
<h6>Our project</h6>
 +
<p>
 +
Ecological power management is currently facing three challenges: the storage of electrical power, the development of renewable energy resources, which are not timely and locally synced with demand, and the increase of atmospheric carbon dioxide due to the use of fossil fuels. We address these challenges by implementing a proof of concept production of the biofuel isobutanol from carbon dioxide. In our project, a bacterial microcompartment from cyanobacteria, the carboxysome, was deployed in <i>Escherichia.&nbsp;coli</i> for carbon dioxide fixation under aerobic conditions. We further engineered <i>E.&nbsp;coli</i> to derive the energy for this process from electricity by implementing electron-mediator uptake and recycling. This ‘electricity’ pathway comprised proteins such as the furmarate reductase. Feasibility was analyzed in a self-constructed bioreactor. Ultimately, we established an isobutanol production pathway by heterologous expression of five genes from <i>E.&nbsp;coli</i>, <i>Bacillus&nbsp;subtilis</i> and <i>Lactococcus&nbsp;lactis</i>.
 +
</p><br>
 +
</div>
 +
</div>
 +
<!--Zusammengesetzte Projektabbildung-->
 +
<div class="element">
 +
  <div id="fullpicture">
 +
  <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/rMFC"style="color:#000000">
 +
      <div id="fullpicture1">
 +
        <p class="ribbon"></p>
 +
      </div>
 +
  </a>
 +
  <div id="fullpicture2">
 +
      <p class="ribbon"></p>
 +
  </div>
 +
  <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/CO2-fixation"style="color:#000000">
 +
      <div id="fullpicture3">
 +
        <p class="ribbon"></p>
 +
      </div>
 +
  </a>
 +
  <div id="fullpicture4">
 +
      <p class="ribbon"></p>
 +
  </div>
 +
  <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Isobutanol"style="color:#000000">
 +
      <div id="fullpicture5">
 +
        <p class="ribbon"></p>
 +
      </div>
 +
  </a>
 +
  </div>
 +
</div>
-
 
+
<div class="element">
-
<br>
+
<div id="text">
-
<br>
+
<h6>Achievements</h6>
-
<br>
+
<p>
-
 
+
<table style="background-color:transparent; cellspacing=3;">
-
<!--gesamte Projektübersicht-Bild-->
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Design and construction of a <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC/Construction" target="_blank">rMFC</a></td></tr>
-
<div id="project_overview"><img src="https://static.igem.org/mediawiki/2014/6/63/Bielefeld-CeBiTec_14-06-01_projekt_overview.png" width="950px" usemap="#Projekt" ;></div>
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Design and construction of a <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC/Construction" target="_blank">flow cell reactor</a></td></tr>
-
 
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Construction of an <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/rMFC/Mediators" target="_blank">electrophilic <i>E.coli</i> strain</a></td></tr>
-
<!--Modul1-Bild-->
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Successful <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Pathway" target="_blank">isobutanol production</a></td></tr>
-
<div id="module_eins"><img src="https://static.igem.org/mediawiki/2014/d/d3/Bielefeld-CeBiTec_2014-06-04_Modul1.png" height="400px";></div>
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Construction of a functional microcompartment, the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Carboxysome" target="_blank">carboxysome</a></td></tr>
-
 
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Genome integration of <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC" target="_blank">OprF</a></td></tr>
-
 
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Functional <i>in-vitro</i> assay of <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO" target"=_blank">RuBisCo</a></td></tr>
-
 
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Development of an <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Biosafety" target="_blank">antibiotic-free selection system</a></td></tr>
-
<map name="Projekt">
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>
-
 
+
Several <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice" target="_blank">human practice projects</a></td></tr>
-
<!-- Link project 1 -->
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>
-
<area id="m1" shape="poly" coords="0,0, 440,0, 390,160, 410,200, 365,400, 0,400"
+
<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Synenergene" target="_blank">SYNENERGENE cooperation</a></td></tr>
-
          href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Module1">
+
<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>
-
 
+
<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Wikisession" target="_blank">Help another team</a></td></tr>
-
<!-- Link to project 2 -->
+
</table>
-
<area id="m2" shape="poly" coords="515,0, 725,0, 670,210, 745,310, 760,400, 440,400, 480,205, 460,165"
+
</p>
-
          href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Module2">
+
</div>
-
 
+
</div>
-
<!-- Link to project 3 -->
+
-
<area id="m3" shape="poly" coords="780,0, 935,0, 935,400, 825,400, 785,260, 725,220"
+
-
          href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Module3">
+
</body>
</body>
-
 
-
</td>
 
-
 
</html>
</html>

Latest revision as of 14:44, 28 November 2014

Welcome to our project


Results of module 1 - rMFC

Results of module 2 - CO2 fixation

Results of module 3 - Isobutanol production

Results of our biosafety

SYNENERGENE/ Applications
Our project

Ecological power management is currently facing three challenges: the storage of electrical power, the development of renewable energy resources, which are not timely and locally synced with demand, and the increase of atmospheric carbon dioxide due to the use of fossil fuels. We address these challenges by implementing a proof of concept production of the biofuel isobutanol from carbon dioxide. In our project, a bacterial microcompartment from cyanobacteria, the carboxysome, was deployed in Escherichia. coli for carbon dioxide fixation under aerobic conditions. We further engineered E. coli to derive the energy for this process from electricity by implementing electron-mediator uptake and recycling. This ‘electricity’ pathway comprised proteins such as the furmarate reductase. Feasibility was analyzed in a self-constructed bioreactor. Ultimately, we established an isobutanol production pathway by heterologous expression of five genes from E. coli, Bacillus subtilis and Lactococcus lactis.


Achievements

Design and construction of a rMFC
Design and construction of a flow cell reactor
Construction of an electrophilic E.coli strain
Successful isobutanol production
Construction of a functional microcompartment, the carboxysome
Genome integration of OprF
Functional in-vitro assay of RuBisCo
Development of an antibiotic-free selection system
Several human practice projects
SYNENERGENE cooperation
Help another team

Retrieved from "http://2014.igem.org/Team:Bielefeld-CeBiTec"