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Team:Evry/Interlab Study/09-13-2014
From 2014.igem.org
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<h2>Other constructions of the Anderson library of constitutive promoters</h2> | <h2>Other constructions of the Anderson library of constitutive promoters</h2> | ||
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| + | <i>PSB1C3+E0240+J23101</i>:<br> | ||
| + | pre-culture of the colony 3 in LB+Cam (1:1000). Incubation 16h at 37°C. | ||
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<i>PSB1C3+E0240+J23105</i>, <i>PSB1C3+E0240+J23106</i> and <i>PSB1C3+E0240+J23107</i>: | <i>PSB1C3+E0240+J23105</i>, <i>PSB1C3+E0240+J23106</i> and <i>PSB1C3+E0240+J23107</i>: | ||
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<li> PCR colony of 7 colonies for each construction following protocol table 1 and 2. | <li> PCR colony of 7 colonies for each construction following protocol table 1 and 2. | ||
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| + | A pre-culture was made with one colony for each construction to make a glycerol stock.<br> | ||
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</ul> | </ul> | ||
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| + | A pre-culture was made with one colony for each construction to make a glycerol stock.<br> | ||
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Latest revision as of 18:56, 17 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101:
pre-culture of the colony 3 in LB+Cam (1:1000). Incubation 16h at 37°C.
PSB1C3+E0240+J23105, PSB1C3+E0240+J23106 and PSB1C3+E0240+J23107:
- PCR colony of 7 colonies for each construction following protocol table 1 and 2.
GEL 1: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23107 construction. PCR product of 8 colonies of the PSB1C3+E0240+J23106 construction.
A pre-culture was made with one colony for each construction to make a glycerol stock.
GEL 2: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23105 construction.
A pre-culture was made with one colony for each construction to make a glycerol stock.
Sep 13
