Team:Evry/Interlab Study/09-13-2014

Construction n°1: PSB1C3 with I20260

Construction n°2: PSB1C3 with J23101-E1010

Construction n°3: PSB1C3 with K823012-E1010

Other constructions of the Anderson library of constitutive promoters

PSB1C3+E0240+J23101:
pre-culture of the colony 3 in LB+Cam (1:1000). Incubation 16h at 37°C.

PSB1C3+E0240+J23105, PSB1C3+E0240+J23106 and PSB1C3+E0240+J23107:

• PCR colony of 7 colonies for each construction following protocol table 1 and 2.
  

  

  GEL 1: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23107 construction. PCR product of 8 colonies of the PSB1C3+E0240+J23106 construction.
  
  A pre-culture was made with one colony for each construction to make a glycerol stock.
  
  
  

  

  GEL 2: 1% agarose gel. PCR product of 7 colonies of the PSB1C3+E0240+J23105 construction.

A pre-culture was made with one colony for each construction to make a glycerol stock.

Sep 13