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Team:Evry/Interlab Study/08-28-2014
From 2014.igem.org
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<br/><h2> Contruction n°3: PSB1C3 with K823012-E1010</h2> | <br/><h2> Contruction n°3: PSB1C3 with K823012-E1010</h2> | ||
| + | <br/>PCR colony was done on 7 colonies: | ||
| + | <ol> | ||
| + | <li>Steril H2O:34,5µL | ||
| + | <li>5X Q5 Reaction buffer: 10µL | ||
| + | <li>Template:2µL | ||
| + | <li>10mM dNTP: 1µL | ||
| + | <li>10µM Primer forward VF2: 1µL | ||
| + | <li>10µM Primer reverse VR: 1µL | ||
| + | <li> Q5 DNA Polymerase: 0,5µL | ||
| + | </ol> | ||
| + | <br/>A migration of PCR product was done. We expect to observe in each wells a band of 1176bp: | ||
| + | <br/><img src="https://static.igem.org/mediawiki/2014/b/b6/Evry2014_J23115-E0240_gel_migration_.jpg" alt="image not found" /> | ||
| + | <br/> | ||
| + | <br/> 6 colonies have a band at 1200 bp around so we decided to choose colonies n°2, 3 and 4. | ||
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<h2>Other constructions of the Anderson library of constitutive promoters</h2> | <h2>Other constructions of the Anderson library of constitutive promoters</h2> | ||
Latest revision as of 02:04, 20 September 2014
Contruction n°2: PSB1C3 with J23101-E1010
Contruction n°3: PSB1C3 with K823012-E1010
PCR colony was done on 7 colonies:
- Steril H2O:34,5µL
- 5X Q5 Reaction buffer: 10µL
- Template:2µL
- 10mM dNTP: 1µL
- 10µM Primer forward VF2: 1µL
- 10µM Primer reverse VR: 1µL
- Q5 DNA Polymerase: 0,5µL
A migration of PCR product was done. We expect to observe in each wells a band of 1176bp:
6 colonies have a band at 1200 bp around so we decided to choose colonies n°2, 3 and 4.
Other constructions of the Anderson library of constitutive promoters
Promoters were resuspended from 2014 distribution kit plates with 10 µl of sterile MiliQ watter and stored at -20°C.
Aug 28
