Team:Caltech/week13

From 2014.igem.org

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<table>
<table>
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<tr><td colspan=2><h1>Week Twelve</h1></td></tr>
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<tr><td colspan=2><h1>Week Thirteen</h1></td></tr>
<tr>
<tr>
<td width="25%"  valign="top">  
<td width="25%"  valign="top">  
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<h4>Friday, 9/5/14</h4>
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<h4>Sunday, 9/7/14</h4>
</td>
</td>
<td valign="top">
<td valign="top">
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<b>Export Systems</b>
 
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<ul>
 
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</ul>
 
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<b>lamBCDA & fsrABC Reception Systems</b>
 
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<ul>
 
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</ul>
 
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<b>agrBCDA Reception System and Combinatorial Promoters</b>
 
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<ul>
 
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</ul>
 
</td>
</td>
</tr>
</tr>
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<h4>Monday, 9/1/14</h4>
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<h4>Monday, 9/8/14</h4>
</td>
</td>
<td valign="top">
<td valign="top">
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<b>Export Systems</b>
 
-
<ul>
 
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</ul>
 
<b>lamBCDA & fsrABC Reception Systems</b>
<b>lamBCDA & fsrABC Reception Systems</b>
<ul>
<ul>
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<h4>Tuesday, 9/2/14</h4>
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<h4>Tuesday, 9/9/14</h4>
</td>
</td>
<td valign="top">
<td valign="top">
<b>Export Systems</b>
<b>Export Systems</b>
-
<ul>
+
<br>Preparation of fsr system for LC/MS analysis:
 +
<ul><li>Made new LB-CARB plates, since the old stock had run out.</li>
 +
    <li>Transformed pTG005-unFLAG into DH5&alpha;. Cells were plated & incubated overnight.</li>
</ul>
</ul>
<b>lamBCDA & fsrABC Reception Systems</b>
<b>lamBCDA & fsrABC Reception Systems</b>
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</ul>
</ul>
<b>agrBCDA Reception System and Combinatorial Promoters</b>
<b>agrBCDA Reception System and Combinatorial Promoters</b>
-
<ul><li>Ran colony PCR using liquid cultures of Gibson colonies inoculated on Friday, in hopes of obtaining clearer gel results</li>
+
<ul>
</ul>
</ul>
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<td width="25%"  valign="top">  
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<h4>Wednesday, 9/3/14</h4>
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<h4>Wednesday, 9/10/14</h4>
</td>
</td>
<td valign="top">
<td valign="top">
<b>Export Systems</b>
<b>Export Systems</b>
-
<ul>
+
<br>Preparation of fsr system for LC/MS analysis:
 +
<ul><li>Set up a liquid culture of pTG005-unFLAG cells.</li>
</ul>
</ul>
<b>lamBCDA & fsrABC Reception Systems</b>
<b>lamBCDA & fsrABC Reception Systems</b>
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</ul>
</ul>
<b>agrBCDA Reception System and Combinatorial Promoters</b>
<b>agrBCDA Reception System and Combinatorial Promoters</b>
-
<ul><li>Ran gel of colony PCR products from yesterday </li>
+
<ul>
-
    <li>Gel still yielded unfavorable results</li>
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    <li>Planning to clone using Golden Gate assembly </li>
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</ul>
</ul>
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<h4>Thursday, 9/4/14</h4>
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<h4>Thursday, 9/11/14</h4>
</td>
</td>
<td valign="top">
<td valign="top">
<b>Export Systems</b>
<b>Export Systems</b>
-
<ul>
+
<br>Preparation of fsr system for LC/MS analysis:
 +
<ul><li>Set up 0 nM, 250 nM, & 500 nM aTc inductions of pTG005-unFLAG expressing cells in MOPS media. 5 μL aliquots of last night's liquid culture was used to start each culture.</li>
 +
    <li>Made a glycerol stock of pTG005-noFLAG</li>
</ul>
</ul>
<b>lamBCDA & fsrABC Reception Systems</b>
<b>lamBCDA & fsrABC Reception Systems</b>
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<tr>
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<td width="25%"  valign="top">  
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<h4>Friday, 9/5/14</h4>
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<h4>Friday, 9/12/14</h4>
</td>
</td>
<td valign="top">
<td valign="top">
<b>Export Systems</b>
<b>Export Systems</b>
-
<ul>
+
<br>Preparation of fsr system for LC/MS analysis:
 +
<ul><li>aTc-induced MOPS liquid cultures from yesterday were spun down, and then the supernatant was loaded into C18 columns, washed with 10% acetonitrile, and then eluted with 60% acetonitrile.</li>
</ul>
</ul>
<b>lamBCDA & fsrABC Reception Systems</b>
<b>lamBCDA & fsrABC Reception Systems</b>
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<tr>
<tr>
<td width="25%"  valign="top">  
<td width="25%"  valign="top">  
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<h4>Saturday, 9/6/14</h4>
+
<h4>Saturday, 9/13/14</h4>
</td>
</td>
<td valign="top">
<td valign="top">
<b>Export Systems</b>
<b>Export Systems</b>
-
<ul>
+
<br>Preparation of fsr system for LC/MS analysis:
-
</ul>
+
<ul><li>Vacufuged pTG005-unFLAG acetonitrile samples to completion.</li>
-
<b>lamBCDA & fsrABC Reception Systems</b>
+
-
<ul>
+
-
</ul>
+
-
<b>agrBCDA Reception System and Combinatorial Promoters</b>
+
-
<ul>
+
</ul>
</ul>
</td>
</td>

Latest revision as of 01:34, 15 September 2014
Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions
Notebook
Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week Thirteen

Sunday, 9/7/14

Monday, 9/8/14

 lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Tuesday, 9/9/14

 Export Systems
Preparation of fsr system for LC/MS analysis:
  • Made new LB-CARB plates, since the old stock had run out.
  • Transformed pTG005-unFLAG into DH5α. Cells were plated & incubated overnight.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Wednesday, 9/10/14

 Export Systems
Preparation of fsr system for LC/MS analysis:
  • Set up a liquid culture of pTG005-unFLAG cells.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Thursday, 9/11/14

 Export Systems
Preparation of fsr system for LC/MS analysis:
  • Set up 0 nM, 250 nM, & 500 nM aTc inductions of pTG005-unFLAG expressing cells in MOPS media. 5 μL aliquots of last night's liquid culture was used to start each culture.
  • Made a glycerol stock of pTG005-noFLAG
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Friday, 9/12/14

 Export Systems
Preparation of fsr system for LC/MS analysis:
  • aTc-induced MOPS liquid cultures from yesterday were spun down, and then the supernatant was loaded into C18 columns, washed with 10% acetonitrile, and then eluted with 60% acetonitrile.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters

Saturday, 9/13/14

 Export Systems
Preparation of fsr system for LC/MS analysis:
  • Vacufuged pTG005-unFLAG acetonitrile samples to completion.

Retrieved from "http://2014.igem.org/Team:Caltech/week13"