Team:Caltech/week12

From 2014.igem.org

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<tr><td colspan=2><h1>Week Twelve</h1></td></tr>
<tr><td colspan=2><h1>Week Twelve</h1></td></tr>
 +
 +
<tr>
 +
<td width="25%"  valign="top">
 +
<h4>Sunday, 8/31/14</h4>
 +
</td>
 +
<td valign="top">
 +
<b>Export Systems</b>
 +
<br>Redo of cloning of agr system:
 +
<ul><li>Ran colony PCR on 10 picked overnight colonies.</li>
 +
    <li>colony PCR products were then run on a gel.</li>
 +
    <li>5 LB-CARB liquid cultures were set up and incubated overnight.</li>
 +
</ul>
 +
De-FLAGging pTG005 for LC/MS analysis:
 +
<ul><li>Ran colony PCR on 10 picked overnight colonies.</li>
 +
    <li>colony PCR products were then run on a gel.</li>
 +
    <li>3 LB-CARB liquid cultures were set up and incubated overnight.</li>
 +
</ul>
 +
</td>
 +
</tr>
 +
 +
<tr><td bgColor="#777777" colspan="2" height="1px"></td></tr>
<tr>
<tr>
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<td valign="top">
<td valign="top">
<b>Export Systems</b>
<b>Export Systems</b>
-
<ul>
+
<br>Redo of cloning of agr system:
-
</ul>
+
<ul><li>Miniprepped the 5 liquid cultures from yesterday. Stored the plasmids in the fridge.</li></ul>
 +
De-FLAGging pTG005 for LC/MS analysis:
 +
<ul><li>Miniprepped the 3 liquid cultures from yesterday. Stored the plasmids in the fridge.</li></ul>
<b>lamBCDA & fsrABC Reception Systems</b>
<b>lamBCDA & fsrABC Reception Systems</b>
-
<ul>
 
-
</ul>
 
-
<b>agrBCDA Reception System and Combinatorial Promoters</b>
 
<ul>
<ul>
</ul>
</ul>
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</td>
</td>
<td valign="top">
<td valign="top">
-
<b>Export Systems</b>
 
-
<ul>
 
-
</ul>
 
<b>lamBCDA & fsrABC Reception Systems</b>
<b>lamBCDA & fsrABC Reception Systems</b>
<ul>
<ul>
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<td valign="top">
<td valign="top">
<b>Export Systems</b>
<b>Export Systems</b>
-
<ul>
+
<ul><li>Shipped samples for both agr-recloning subproject (2*5=10 samples) & pTG005-unFLAGging subproject (2*3=6 samples) out for sequencing.</li>
</ul>
</ul>
<b>lamBCDA & fsrABC Reception Systems</b>
<b>lamBCDA & fsrABC Reception Systems</b>
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<td valign="top">
<td valign="top">
<b>Export Systems</b>
<b>Export Systems</b>
-
<ul>
+
<ul><li>Sequencing data appears to confirm successful unFLAGging of pTG005.</li>
-
</ul>
+
-
<b>lamBCDA & fsrABC Reception Systems</b>
+
-
<ul>
+
-
</ul>
+
-
<b>agrBCDA Reception System and Combinatorial Promoters</b>
+
-
<ul>
+
</ul>
</ul>
</td>
</td>
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</td>
</td>
<td valign="top">
<td valign="top">
-
<b>Export Systems</b>
+
</td>
-
<ul>
+
</tr>
-
</ul>
+
 
-
<b>lamBCDA & fsrABC Reception Systems</b>
+
<tr><td bgColor="#777777" colspan="2" height="1px"></td></tr>
-
<ul>
+
 
-
</ul>
+
<tr>
-
<b>agrBCDA Reception System and Combinatorial Promoters</b>
+
<td width="25%"  valign="top">  
-
<ul>
+
<h4>Saturday, 9/6/14</h4>
-
</ul>
+
</td>
 +
<td valign="top">
</td>
</td>
</tr>
</tr>

Latest revision as of 01:02, 15 September 2014
Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions
Notebook
Overview

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week Twelve

Sunday, 8/31/14

 Export Systems
Redo of cloning of agr system:
  • Ran colony PCR on 10 picked overnight colonies.
  • colony PCR products were then run on a gel.
  • 5 LB-CARB liquid cultures were set up and incubated overnight.
De-FLAGging pTG005 for LC/MS analysis:
  • Ran colony PCR on 10 picked overnight colonies.
  • colony PCR products were then run on a gel.
  • 3 LB-CARB liquid cultures were set up and incubated overnight.

Monday, 9/1/14

 Export Systems
Redo of cloning of agr system:
  • Miniprepped the 5 liquid cultures from yesterday. Stored the plasmids in the fridge.
De-FLAGging pTG005 for LC/MS analysis:
  • Miniprepped the 3 liquid cultures from yesterday. Stored the plasmids in the fridge.
lamBCDA & fsrABC Reception Systems

Tuesday, 9/2/14

 lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
  • Ran colony PCR using liquid cultures of Gibson colonies inoculated on Friday, in hopes of obtaining clearer gel results

Wednesday, 9/3/14

 Export Systems
  • Shipped samples for both agr-recloning subproject (2*5=10 samples) & pTG005-unFLAGging subproject (2*3=6 samples) out for sequencing.
lamBCDA & fsrABC Reception Systems
agrBCDA Reception System and Combinatorial Promoters
  • Ran gel of colony PCR products from yesterday
  • Gel still yielded unfavorable results
  • Planning to clone using Golden Gate assembly

Thursday, 9/4/14

 Export Systems
  • Sequencing data appears to confirm successful unFLAGging of pTG005.

Friday, 9/5/14

Saturday, 9/6/14

Retrieved from "http://2014.igem.org/Team:Caltech/week12"