Team:Bielefeld-CeBiTec

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<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project"style="color:#000000">Project </a> </td>
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<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results"style="color:#000000">Results </a> </td>
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<h1>Welcome to our project </h1>
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      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC" target="_blank"><img src="https://static.igem.org/mediawiki/2014/d/d2/Bielefeld-CeBiTec_14-10-16_Mediator_NeutralRed.png" width="155px"></a><br>
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      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC" target="_blank"><b>Results of module 1 - rMFC</b></a></font>
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      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation" target="_blank"><img src="https://static.igem.org/mediawiki/2014/c/c7/Bielefeld-CeBiTec_2014-10-12_Carboxysom.png" width="155px"></a><br>
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      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation" target="_blank"><b>Results of module 2 - CO<sub>2</sub> fixation</b></a></font>
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      <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Isobutanol" target="_blank"><img src="https://static.igem.org/mediawiki/2014/1/11/Bielefeld-CeBiTec_14-10-17_IsobutProd_37.png" width="155px"></a><br>
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      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Isobutanol" target="_blank"><b>Results of module 3 - Isobutanol production</b></a></font>
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      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Biosafety" target="_blank"><b>Results of our biosafety</b></a></font>
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      <font size="2" style="text-align:center;"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Synenergene" target="_blank"><b>SYNENERGENE/ Applications</b></a></font>
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<h6>Our project</h6>
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Ecological power management is currently facing three challenges: the storage of electrical power, the development of renewable energy resources, which are not timely and locally synced with demand, and the increase of atmospheric carbon dioxide due to the use of fossil fuels. We address these challenges by implementing a proof of concept production of the biofuel isobutanol from carbon dioxide. In our project, a bacterial microcompartment from cyanobacteria, the carboxysome, was deployed in <i>Escherichia.&nbsp;coli</i> for carbon dioxide fixation under aerobic conditions. We further engineered <i>E.&nbsp;coli</i> to derive the energy for this process from electricity by implementing electron-mediator uptake and recycling. This ‘electricity’ pathway comprised proteins such as the furmarate reductase. Feasibility was analyzed in a self-constructed bioreactor. Ultimately, we established an isobutanol production pathway by heterologous expression of five genes from <i>E.&nbsp;coli</i>, <i>Bacillus&nbsp;subtilis</i> and <i>Lactococcus&nbsp;lactis</i>.
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<h6>Achievements</h6>
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<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Design and construction of a <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC/Construction" target="_blank">rMFC</a></td></tr>
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<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Design and construction of a <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC/Construction" target="_blank">flow cell reactor</a></td></tr>
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<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Construction of an <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/rMFC/Mediators" target="_blank">electrophilic <i>E.coli</i> strain</a></td></tr>
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<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Successful <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Pathway" target="_blank">isobutanol production</a></td></tr>
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<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Construction of a functional microcompartment, the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Carboxysome" target="_blank">carboxysome</a></td></tr>
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<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Genome integration of <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC" target="_blank">OprF</a></td></tr>
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<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Functional <i>in-vitro</i> assay of <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO" target"=_blank">RuBisCo</a></td></tr>
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<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Development of an <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Biosafety" target="_blank">antibiotic-free selection system</a></td></tr>
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<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>
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Several <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice" target="_blank">human practice projects</a></td></tr>
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<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>
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<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Synenergene" target="_blank">SYNENERGENE cooperation</a></td></tr>
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<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>
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<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Wikisession" target="_blank">Help another team</a></td></tr>
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Latest revision as of 14:44, 28 November 2014

Welcome to our project


Results of module 1 - rMFC

Results of module 2 - CO2 fixation

Results of module 3 - Isobutanol production

Results of our biosafety

SYNENERGENE/ Applications
Our project

Ecological power management is currently facing three challenges: the storage of electrical power, the development of renewable energy resources, which are not timely and locally synced with demand, and the increase of atmospheric carbon dioxide due to the use of fossil fuels. We address these challenges by implementing a proof of concept production of the biofuel isobutanol from carbon dioxide. In our project, a bacterial microcompartment from cyanobacteria, the carboxysome, was deployed in Escherichia. coli for carbon dioxide fixation under aerobic conditions. We further engineered E. coli to derive the energy for this process from electricity by implementing electron-mediator uptake and recycling. This ‘electricity’ pathway comprised proteins such as the furmarate reductase. Feasibility was analyzed in a self-constructed bioreactor. Ultimately, we established an isobutanol production pathway by heterologous expression of five genes from E. coli, Bacillus subtilis and Lactococcus lactis.


Achievements

Design and construction of a rMFC
Design and construction of a flow cell reactor
Construction of an electrophilic E.coli strain
Successful isobutanol production
Construction of a functional microcompartment, the carboxysome
Genome integration of OprF
Functional in-vitro assay of RuBisCo
Development of an antibiotic-free selection system
Several human practice projects
SYNENERGENE cooperation
Help another team

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