Team:TU Eindhoven/Project/protocols

From 2014.igem.org

(Difference between revisions)

Latest revision as of 19:19, 31 August 2014

Project Description

Protocols

Introduction

For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.

genetic engineering protocols

The following protocols are used in the Biolab during the modification of bacteria.

Colony PCR Antibody Titration with FACS

FACS for sorting with DBCO-TAMRA

FACS for sorting with DBCO-PEG(10kDa)

Vector Ligation

Overhang PCR

PCR purification of DNA fragments

Plasmid Amplification

Plasmid Gene Digestion

Plasmid Purification

Preparative Steps

Protein Expression Curve

Protein Expression

Site Directed Mutagenesis

Vector Transformation

chemistry protocols

SPAAC reaction monitoring with UV-Visible Spectroscopy

microfluidics protocols

Here will follow all microfluidic engineering protocols

@@ Line 58: / Line 58: @@
 	       <div class="Jasper">
 	   <span>genetic engineering protocols</span>
-<br>
+<br>   	<p class="para">The following protocols are used in the Biolab during the modification of bacteria.
-	       	<p class="para"><a href="https://static.igem.org/mediawiki/2014/6/6e/Protocol_-_Colony_PCR.pdf">Colony PCR</a></p>
+<br><br>
+  </p>
+	       	<p class="para"><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6e/Protocol_-_Colony_PCR.pdf">Colony PCR</a>
-<br> <br>
+<a target="_blank" href="https://static.igem.org/mediawiki/2014/b/b1/Protocol_-_FACS_%28Antibody_Titration%29.pdf">Antibody Titration with FACS</a>
-<a href="https://static.igem.org/mediawiki/2014/b/b1/Protocol_-_FACS_%28Antibody_Titration%29.pdf">Antibody Titration with FACS</a></p>
+<br><br>
-<a href="https://static.igem.org/mediawiki/2014/7/7e/Protocol_-_FACS_%28DBCO-56-TAMRA%29.pdf">FACS for sorting with DBCO-TAMRA</a></p>
+<a target="_blank"  href="https://static.igem.org/mediawiki/2014/7/7e/Protocol_-_FACS_%28DBCO-56-TAMRA%29.pdf">FACS for sorting with DBCO-TAMRA</a>
+<br><br>
-<a href="https://static.igem.org/mediawiki/2014/f/f0/Protocol_-_FACS_%28DBCO-PEG_10_kDa%29.pdf">FACS for sorting with DBCO-PEG(10kDa)</a></p>
+<a target="_blank" href="https://static.igem.org/mediawiki/2014/f/f0/Protocol_-_FACS_%28DBCO-PEG_10_kDa%29.pdf">FACS for sorting with DBCO-PEG(10kDa)</a>
+<br><br>
-<a href="https://static.igem.org/mediawiki/2014/a/ae/Protocol_-_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a></p>
+<a target="_blank" href="https://static.igem.org/mediawiki/2014/a/ae/Protocol_-_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a></p>
-<a href="https://static.igem.org/mediawiki/2014/8/81/Protocol_-_Overhang_PCR.pdf">Overhang PCR</a></p>
+<a target="_blank" href="https://static.igem.org/mediawiki/2014/8/81/Protocol_-_Overhang_PCR.pdf">Overhang PCR</a></p>
-<a href="https://static.igem.org/mediawiki/2014/0/0a/Protocol_-_PCR_Purification_of_Insert_Fragment.pdf">PCR purification of DNA fragments</a></p>
+<a target="_blank"  href="https://static.igem.org/mediawiki/2014/0/0a/Protocol_-_PCR_Purification_of_Insert_Fragment.pdf">PCR purification of DNA fragments</a></p>
-<a href="https://static.igem.org/mediawiki/2014/2/22/Protocol_-_Plasmid_Amplification.pdf">Plasmid Amplification</a></p>
+<a target="_blank" href="https://static.igem.org/mediawiki/2014/2/22/Protocol_-_Plasmid_Amplification.pdf">Plasmid Amplification</a></p>
-<a href="
+<a target="_blank" href="
 https://static.igem.org/mediawiki/2014/0/07/Protocol_-_Plasmid_and_gene_digestion.pdf">Plasmid Gene Digestion</a></p>
-<a href="
+<a target="_blank" href="
 https://static.igem.org/mediawiki/2014/2/29/Protocol_-_Plasmid_purification.pdf">Plasmid Purification</a></p>
-<a href="
+<a target="_blank" href="
 https://static.igem.org/mediawiki/2014/1/13/Protocol_-_Preparative_steps.pdf">Preparative Steps</a></p>
-<a href="
+<a target="_blank" href="
 https://static.igem.org/mediawiki/2014/f/f8/Protocol_-_Protein_Expression_Curve.pdf">Protein Expression Curve</a></p>
-<a href="
+<a target="_blank" href="
 https://static.igem.org/mediawiki/2014/4/45/Protocol_-_Protein_expression.pdf">Protein Expression</a></p>
-<a href="
+<a target="_blank" href="
 https://static.igem.org/mediawiki/2014/6/62/Protocol_-_Site_Directed_Mutagenesis.pdf">Site Directed Mutagenesis</a></p>
-<a href="
+<a target="_blank" href="
-https://static.igem.org/mediawiki/2014/b/b5/Protocol_-_Transformation.pdf">Transformation</a></p>
+https://static.igem.org/mediawiki/2014/b/b5/Protocol_-_Transformation.pdf">Vector Transformation</a></p>
@@ Line 112: / Line 118: @@
 	       <div class="Jasper">
 	   <span>chemistry protocols</span>
-	       	<p class="para">Here will follow all genetic engineering protocols</p>
+	       	<p class="para">
+<a href="
+https://static.igem.org/mediawiki/2014/3/3d/Protocol_-_Monitoring_SPAAC_with_UVVIS.pdf">SPAAC reaction monitoring with UV-Visible Spectroscopy</a></p>
 		</div>
 	      	 <div class="clear"></div>
@@ Line 127: / Line 138: @@
 	       <div class="Jasper">
 	   <span>microfluidics protocols</span>
-	       	<p class="para">Here will follow all genetic engineering protocols</p>
+	       	<p class="para">Here will follow all microfluidic engineering protocols</p>
 		</div>
 	      	 <div class="clear"></div>