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Team:TU Eindhoven/Project/protocols
From 2014.igem.org
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<a href="https://static.igem.org/mediawiki/2014/7/7e/Protocol_-_FACS_%28DBCO-56-TAMRA%29.pdf">FACS for sorting with DBCO-TAMRA</a></p> | <a href="https://static.igem.org/mediawiki/2014/7/7e/Protocol_-_FACS_%28DBCO-56-TAMRA%29.pdf">FACS for sorting with DBCO-TAMRA</a></p> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/f/f0/Protocol_-_FACS_%28DBCO-PEG_10_kDa%29.pdf">FACS for sorting with DBCO-PEG(10kDa)</a></p> | ||
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| + | <a href="https://static.igem.org/mediawiki/2014/a/ae/Protocol_-_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a></p> | ||
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| + | <a href="https://static.igem.org/mediawiki/2014/8/81/Protocol_-_Overhang_PCR.pdf">Overhang PCR</a></p> | ||
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| + | <a href="https://static.igem.org/mediawiki/2014/0/0a/Protocol_-_PCR_Purification_of_Insert_Fragment.pdf">PCR purification of DNA fragments</a></p> | ||
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| + | <a href="https://static.igem.org/mediawiki/2014/2/22/Protocol_-_Plasmid_Amplification.pdf">Plasmid Amplification</a></p> | ||
<a href=" | <a href=" | ||
| - | https://static.igem.org/mediawiki/2014/f/ | + | https://static.igem.org/mediawiki/2014/0/07/Protocol_-_Plasmid_and_gene_digestion.pdf">Plasmid Gene Digestion</a></p> |
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| + | <a href=" | ||
| + | https://static.igem.org/mediawiki/2014/2/29/Protocol_-_Plasmid_purification.pdf">Plasmid Purification</a></p> | ||
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| + | <a href=" | ||
| + | https://static.igem.org/mediawiki/2014/1/13/Protocol_-_Preparative_steps.pdf">Preparative Steps</a></p> | ||
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| + | <a href=" | ||
| + | https://static.igem.org/mediawiki/2014/f/f8/Protocol_-_Protein_Expression_Curve.pdf">Protein Expression Curve</a></p> | ||
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| + | <a href=" | ||
| + | https://static.igem.org/mediawiki/2014/4/45/Protocol_-_Protein_expression.pdf">Protein Expression</a></p> | ||
| + | <a href=" | ||
| + | https://static.igem.org/mediawiki/2014/6/62/Protocol_-_Site_Directed_Mutagenesis.pdf">Site Directed Mutagenesis</a></p> | ||
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| + | <a href=" | ||
| + | https://static.igem.org/mediawiki/2014/b/b5/Protocol_-_Transformation.pdf">Transformation</a></p> | ||
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</div> | </div> | ||
Revision as of 18:19, 30 August 2014
Protocols
Introduction
For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.
genetic engineering protocols
Antibody Titration with FACS FACS for sorting with DBCO-TAMRA FACS for sorting with DBCO-PEG(10kDa) Vector Ligation Overhang PCR PCR purification of DNA fragments Plasmid Amplification Plasmid Gene Digestion Plasmid Purification Preparative Steps Protein Expression Curve Protein Expression Site Directed Mutagenesis Transformation
Antibody Titration with FACS FACS for sorting with DBCO-TAMRA FACS for sorting with DBCO-PEG(10kDa) Vector Ligation Overhang PCR PCR purification of DNA fragments Plasmid Amplification Plasmid Gene Digestion Plasmid Purification Preparative Steps Protein Expression Curve Protein Expression Site Directed Mutagenesis Transformation
chemistry protocols
Here will follow all genetic engineering protocols
microfluidics protocols
Here will follow all genetic engineering protocols
