Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Aug

From 2014.igem.org

(Difference between revisions)
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             <div class="content">
             <div class="content">
             <ul>
             <ul>
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        <li><b><i>kivD</i>, <i>alsS</i>, <i>ilvC</i> and <i>ilvD</i></b></li>
+
        <li><b>psB1C3-<i>alsS</i>-<i>ilvC</i>-<i>ilvD</i></b></li>
        <ul>
        <ul>
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  <li>This week we wanted to identify our positive colonies.</li>
+
  <li>This week we wanted to identify positive colonies.</li>
                   <ul>
                   <ul>
                       <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ColonyPCR" target="_blank">Colony PCR</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_alsS_ilvC" target="_blank">fw_alsS_ilvC</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_kivD_ilvD" target="_blank">rv_kivD_ilvD</a>)</li>
                       <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ColonyPCR" target="_blank">Colony PCR</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_alsS_ilvC" target="_blank">fw_alsS_ilvC</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_kivD_ilvD" target="_blank">rv_kivD_ilvD</a>)</li>
              <ul>
              <ul>
        <li>Annealing temperature: 65°C</li>
        <li>Annealing temperature: 65°C</li>
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        <li>Bands not as expected (... bp)</li>
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        <li>Bands not as expected 3,45/li>
              </ul>
              </ul>
                   </ul>
                   </ul>
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             <div class="content">
             <div class="content">
             <ul>
             <ul>
-
        <li><b><i>kivD</i>, <i>alsS</i>, <i>ilvC</i>, <i>ilvD</i> and backbone of pSB1C3</b></li>
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        <li><b><i>alsS</i>, <i>ilvC</i>, <i>ilvD</i>, <i>kivD</i> and backbone of pSB1C3</b></li>
        <ul>
        <ul>
  <li>We started again back by zero to solve our problems.</li>
  <li>We started again back by zero to solve our problems.</li>
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        <li>Bands (not) as expected (... bp)</li>
        <li>Bands (not) as expected (... bp)</li>
              </ul>
              </ul>
-
                       <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson" target="_blank">Gibson Assembly</a> with <i>kivD</i>, <i>alsS</i>, <i>ilvC</i> and <i>ilvD</i> on pSB1C3</li>
+
                       <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson" target="_blank">Gibson Assembly</a> with <i>alsS</i>, <i>ilvC</i>, <i>ilvD</i> and <i>kivD</i> on pSB1C3</li>
                       <li>Transformation with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">electrocompotetent cells</a></li>
                       <li>Transformation with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">electrocompotetent cells</a></li>
                       <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ColonyPCR" target="_blank">Colony PCR</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_alsS_ilvC" target="_blank">fw_alsS_ilvC</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_kivD_ilvD" target="_blank">rv_kivD_ilvD</a>)</li>
                       <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ColonyPCR" target="_blank">Colony PCR</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_alsS_ilvC" target="_blank">fw_alsS_ilvC</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_kivD_ilvD" target="_blank">rv_kivD_ilvD</a>)</li>
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             <div class="content">
             <div class="content">
               <ul>
               <ul>
-
        <li><b><i>kivD</i>, <i>alsS</i>, <i>ilvC</i>, <i>ilvD</i> and backbone pSB1C3</b></li>
+
        <li><b><i>alsS</i>, <i>ilvC</i>, <i>ilvD</i>, <i>kivD</i> and backbone pSB1C3</b></li>
        <ul>
        <ul>
    <li>This week we tried to begin from zero again with optimized conditions and new competent cells.</li>
    <li>This week we tried to begin from zero again with optimized conditions and new competent cells.</li>

Revision as of 16:19, 30 August 2014


August

  • psB1C3-alsS-ilvC-ilvD
  • alsS, ilvC, ilvD, kivD and backbone pSB1C3