Team:Evry/Interlab Study/08-23-2014

From 2014.igem.org

(Difference between revisions)
Line 6: Line 6:
     <div class="cd-timeline-content">
     <div class="cd-timeline-content">
       <p align="justify">
       <p align="justify">
-
 
+
<h2>Contruction n°1: PSB1C3 with I20260</h2>
-
<u> Contruction n°1: PSB1C3 with I20260</u>
+
<br/>Digestion of PSB1C3 and PSB1K3-I20260 by EcoRI and PstI
<br/>Digestion of PSB1C3 and PSB1K3-I20260 by EcoRI and PstI
<br/>
<br/>
Line 14: Line 13:
<br/>
<br/>
-
<br/><u> Contruction n°2: PSB1C3 with J23101-E1010</u>
+
<br/><h2>Contruction n°2: PSB1C3 with J23101-E1010</h2>
<br/>
<br/>
<br/>
<br/>
-
<br/><u> Contruction n°3: PSB1C3 with K823012-E1010</u>
+
<br/><h2> Contruction n°3: PSB1C3 with K823012-E1010</h2>
<br/>
<br/>

Revision as of 16:18, 28 August 2014

Picture

Contruction n°1: PSB1C3 with I20260


Digestion of PSB1C3 and PSB1K3-I20260 by EcoRI and PstI

Enzyme inactivation: heating 20 minutes at 80°C
Loading on a 1% agarose gel to perform an extraction from gel

Contruction n°2: PSB1C3 with J23101-E1010




Contruction n°3: PSB1C3 with K823012-E1010


Aug 23