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Team:Bielefeld-CeBiTec/Notebook/Journal/C02-fixation/Jul
From 2014.igem.org
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| + | <ul> | ||
| + | <li><b>SBPase (glpX)</b></li> | ||
| + | <ul> | ||
| + | <li>This week we tried to amplify both parts of glpX.</li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#Primer1" target="_blank">Primer1</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#Primer2" target="_blank">Primer2</a> and <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#Primer3" target="_blank">Primer3</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#Primer4" target="_blank">Primer4</a>)</li> | ||
| + | <ul> | ||
| + | <li>Annealing temperature: ...</li> | ||
| + | <li>Bands (not) as expected (... bp)</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | <li><b>Carbnonic anhydrase (can)</b></li> | ||
| + | <ul> | ||
| + | <li>This week we tried to transform the construct.</li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson" target="_blank">Gibson Assembly</a> with csoS1D and pSB1C3</li> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#RestrictionDigestion" target="_blank">Restriction digestion</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>DpnI</i></a></li> | ||
| + | <ul> | ||
| + | <li>Bands (not) as expected (... bp)</li> | ||
| + | </ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ColonyPCR" target="_blank">Colony PCR</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#Primer1" target="_blank">Primer1</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#Primer2" target="_blank">Primer2</a>)</li> | ||
| + | <ul> | ||
| + | <li>Annealing temperature: ...</li> | ||
| + | <li>Bands as expected (1900 bp)</li> | ||
| + | </ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of can</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | <li><b>csoS1D</b></li> | ||
| + | <ul> | ||
| + | <li>This week we tried to transform the construct.</li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson" target="_blank">Gibson Assembly</a> with csoS1D and pSB1C3</li> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#RestrictionDigestion" target="_blank">Restriction digestion</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>DpnI</i></a></li> | ||
| + | <ul> | ||
| + | <li>Bands (not) as expected (... bp)</li> | ||
| + | </ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ColonyPCR" target="_blank">Colony PCR</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#Primer1" target="_blank">Primer1</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#Primer2" target="_blank">Primer2</a>)</li> | ||
| + | <ul> | ||
| + | <li>Annealing temperature: ...</li> | ||
| + | <li>Bands as expected (1000 bp)</li> | ||
| + | </ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#RestrictionDigestion" target="_blank">Restriction digestion</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>EcoRI</i></a> and <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>XbaI</i></a></li> | ||
| + | <ul> | ||
| + | <li>Bands (not) as expected (... bp)</li> | ||
| + | </ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of csoS1D</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | <li><b>Phosphoribulokinase (prkA)</b></li> | ||
| + | <ul> | ||
| + | <li>This week we tried to transform the fragments of the gene synthesis</li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of prkA</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | <li><b>sRNA:pfkA</b></li> | ||
| + | <ul> | ||
| + | <li>This week we tried to transform the fragments of the gene synthesis</li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of sRNA:pfkA</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | <li><b>RuBisCO of <i>H. neapolitanus</i></b></li> | ||
| + | <ul> | ||
| + | <li>This week we tried to transform the fragments of the gene synthesis</li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of Hneap</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | <li><b>RuBisCO of <i>S. elongatus</i></b></li> | ||
| + | <ul> | ||
| + | <li>This week we tried to transform the fragments of the gene synthesis</li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | <li><b>BioBricks (BBa_I719005)</b></li> | ||
| + | <ul> | ||
| + | <li>This week we tried to use the promotor of the BioBrick of the parts distribution.</li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of pSB1C3_T7 BBa_I719005</li> | ||
| + | </ul> | ||
| + | </ul> | ||
| + | |||
| + | </ul> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 10:02, 28 August 2014
 |
July |
 |
- pSB1C3 backbone
- This week we tried to amplify parts for the Gibson Assembly
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (~2070 bp)
- Purification of the backbones with gel extraction
- pHnCBcsoS1D
- This week we aimed to isolate the plasmid of the strain ordered from addgene
- Plasmid isolation of pHnCBcsoS1D
- csoS1D of the carboxysome
- This week we tried to amplify different parts of the carboxysome.
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (~703 bp)
- Carbonic anhydrase (can) of the carboxysome
- This week we tried to amplify different parts of the carboxysome.
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands (not) as expected (... bp)
- Shell associated protein of the carboxysome (first protein)
- This week we tried to amplify different parts of the carboxysome.
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (... bp)
- csoS1D
- This week we tried to transform the construct.
- Gibson Assembly with csoS1D on pSB1C3
- Transformation with electrocompotetent cells
- Colony PCR (Primer1, Primer2)
- Annealing temperature: ...
- Bands not as expected (... bp). Size looked like template insert CFP.
- Restriction digestion with DpnI
- Bands (not) as expected (... bp)
- Carbonic anhydrase (can)
- This week we tried to transform the construct.
- Gibson Assembly with can and pSB1C3
- Transformation with electrocompotetent cells
- Transformation was not succesful.
- pHnCBcsoS1D backbone
- This week we tried to amplify the backbone of the plasmid.
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (~13,200 bp)
- BioBricks (BBa_k731500 and BBa_Q01400)
- This week we tried to use promotors of two BioBricks of the parts distribution.
- Plasmid isolation of pSB1C3_pTac and pSB1C3_p_TetR
- csoS4AB
- This week we tried amplifiy shell proteins of the carboxysome.
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (... bp)
- csoS1CAB
- This week we tried amplifiy shell proteins of the carboxysome.
- PCR amplification (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (... bp)
- Carbonic anhydrase
- This week we tried to purify the construct from the gel.
- Purification of can with gel extraction
- Bands not as expected (... bp)
- Shell associated protein
- This week we tried to purify the construct from the gel.
- Purification of can with gel extraction
- Bands as expected (~1235 bp)
- Pore protein (csoS1D) of the carboxysom
- This week we tried to purify the construct from the gel.
- Purification of can with gel extraction
- Bands not as expected (~703 bp)
- SBPase (glpX)
- This week we tried to amplify both parts of glpX.
- PCR amplification (Primer1, Primer2 and Primer3, Primer4)
- Annealing temperature: ...
- Bands (not) as expected (... bp)
- Carbnonic anhydrase (can)
- This week we tried to transform the construct.
- Gibson Assembly with csoS1D and pSB1C3
- Restriction digestion with DpnI
- Bands (not) as expected (... bp)
- Colony PCR (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (1900 bp)
- Plasmid isolation of can
- csoS1D
- This week we tried to transform the construct.
- Gibson Assembly with csoS1D and pSB1C3
- Restriction digestion with DpnI
- Bands (not) as expected (... bp)
- Colony PCR (Primer1, Primer2)
- Annealing temperature: ...
- Bands as expected (1000 bp)
- Restriction digestion with EcoRI and XbaI
- Bands (not) as expected (... bp)
- Plasmid isolation of csoS1D
- Phosphoribulokinase (prkA)
- This week we tried to transform the fragments of the gene synthesis
- Transformation with electrocompotetent cells
- Plasmid isolation of prkA
- sRNA:pfkA
- This week we tried to transform the fragments of the gene synthesis
- Transformation with electrocompotetent cells
- Plasmid isolation of sRNA:pfkA
- RuBisCO of H. neapolitanus
- This week we tried to transform the fragments of the gene synthesis
- Transformation with electrocompotetent cells
- Plasmid isolation of Hneap
- RuBisCO of S. elongatus
- This week we tried to transform the fragments of the gene synthesis
- Transformation with electrocompotetent cells
- BioBricks (BBa_I719005)
- This week we tried to use the promotor of the BioBrick of the parts distribution.
- Plasmid isolation of pSB1C3_T7 BBa_I719005
