Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Jun

From 2014.igem.org

(Difference between revisions)
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      <li><b><i>kivD</i></b></li>
      <li><b><i>kivD</i></b></li>
      <ul>
      <ul>
-
  <li>This week we tried to combine kivD with the other using the Gibson Assembly.</li>
+
  <li>This week we tried to combine <i>kivD</i> with the other using the Gibson Assembly.</li>
                   <ul>
                   <ul>
                     <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_ilvD_kivD" target="_blank">fw_ilvD_kivD</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_pSB1C3_kivD" target="_blank">rv_pSB1C3_kivD</a>)</li>
                     <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_ilvD_kivD" target="_blank">fw_ilvD_kivD</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_pSB1C3_kivD" target="_blank">rv_pSB1C3_kivD</a>)</li>
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               <li><b><i>alsS</i></b></li>
               <li><b><i>alsS</i></b></li>
      <ul>
      <ul>
-
  <li>This week we tried to combine alsS with the other using the Gibson Assembly.</li>
+
  <li>This week we tried to combine <i>alsS</i> with the other using the Gibson Assembly.</li>
                   <ul>
                   <ul>
                     <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_alsS_pSB1C3" target="_blank">fw_alsS_pSB1C3</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_ilvC_alsS" target="_blank">rv_ilvC_alsS</a>)</li>
                     <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_alsS_pSB1C3" target="_blank">fw_alsS_pSB1C3</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_ilvC_alsS" target="_blank">rv_ilvC_alsS</a>)</li>
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               <li><b><i>ilvC</i></b></li>
               <li><b><i>ilvC</i></b></li>
      <ul>
      <ul>
-
  <li>This week we tried to combine ilvC with the other using the Gibson Assembly.</li>
+
  <li>This week we tried to combine <i>ilvC</i> with the other using the Gibson Assembly.</li>
                   <ul>
                   <ul>
                     <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_alsS_ilvC" target="_blank">fw_alsS_ilvC</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_ilvD_ilvC" target="_blank">rv_ilvD_ilvC</a>)</li>
                     <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PCR" target="_blank">PCR amplification</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_alsS_ilvC" target="_blank">fw_alsS_ilvC</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_ilvD_ilvC" target="_blank">rv_ilvD_ilvC</a>)</li>

Revision as of 10:33, 27 August 2014


June

Week 4     06/23 - 06/29
Week 4     06/23 - 06/29
  • kivD
    • This week we aim to transform the construct from the parts distribution.
      • Transformation with electrocompotetent cells
        • K538742: pSB1C3-kivD (parts distribution from 2013, plate 1, 24L)
      • Plasmid isolation of kivD
      • Sequencing confirmed identities of all parts, but not whole sequence of the parts was covered. (VF and VR)
      • Glycerin stock created: E. coli KRX pSB1C3-kivD
  • alsS
    • This week we aim to transform the construct from the parts distribution.
      • Transformation with electrocompotetent cells
        • K539627: pSB1C3-alsS (parts distribution from 2013, plate 1, 24H)
      • Plasmid isolation of alsS
      • Sequencing confirmed identities of all parts, but not whole sequence of the parts was covered. (VF and VR)
      • Glycerin stock created: E. coli KRX pSB1C3-alsS
  • ilvC
    • This week we aim to transform the construct from the parts distribution.
      • Transformation with electrocompotetent cells
        • K539621: pSB1C3-ilvC (parts distribution from 2013, plate 1, 24F)
      • Plasmid isolation of ilC
      • Sequencing confirmed identities of all parts, but not whole sequence of the parts was covered. (VF and VR)
      • Glycerin stock created: E. coli KRX pSB1C3-ilvC
  • ilvD
    • This week we aim to transform the construct from the parts distribution.
      • Transformation with electrocompotetent cells
        • K539626: pSB1C3-ilvD (parts distribution from 2013, plate 1, 22J)
      • Plasmid isolation of ilvD
      • Sequencing confirmed identities of all parts, but not whole sequence of the parts was covered. (VF and VR)
      • Glycerin stock created: E. coli KRX pSB1C3-ilvD
Week 5     06/30 - 07/06
Week 5 06/30 - 07/06

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