"
Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Jun
From 2014.igem.org
(Difference between revisions)
(some experiments of week 4 added) |
|||
| Line 104: | Line 104: | ||
<ul> | <ul> | ||
<li>Transformation with electrocompotetent <i> E.coli </i> KRX cells:</li> | <li>Transformation with electrocompotetent <i> E.coli </i> KRX cells:</li> | ||
| - | < | + | <ul> |
<li>K538742: <i>kivD</i> (parts distribution from 2013, plate 1, 24L)</li> | <li>K538742: <i>kivD</i> (parts distribution from 2013, plate 1, 24L)</li> | ||
<li>K539621: <i>ilvC</i> (parts distribution from 2013, plate 1, 24F)</li> | <li>K539621: <i>ilvC</i> (parts distribution from 2013, plate 1, 24F)</li> | ||
<li>K539626: <i>ilvD</i> (parts distribution from 2013, plate 1, 22J)</li> | <li>K539626: <i>ilvD</i> (parts distribution from 2013, plate 1, 22J)</li> | ||
<li>K539627: <i>alsS</i> (parts distribution from 2013, plate 1, 24H)</li> | <li>K539627: <i>alsS</i> (parts distribution from 2013, plate 1, 24H)</li> | ||
| - | </ | + | </ul> |
<li> Resulting colonies were selected to start liquid cultures: </li> | <li> Resulting colonies were selected to start liquid cultures: </li> | ||
| - | < | + | <ul> |
<li>5 ml of LB in 20 ml reaction tube</li> | <li>5 ml of LB in 20 ml reaction tube</li> | ||
<li>incubation over night at 37°C and 250 rpm</li> | <li>incubation over night at 37°C and 250 rpm</li> | ||
| - | </ | + | </ul> |
<li>Plasmid isolation by ThermoScientifici MiniPrep</li> | <li>Plasmid isolation by ThermoScientifici MiniPrep</li> | ||
<li>Sanger sequencing using <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VF" target="_blank">VF</a> and <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VR" target="_blank">VR</a> as sequencing primers</li> | <li>Sanger sequencing using <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VF" target="_blank">VF</a> and <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#VR" target="_blank">VR</a> as sequencing primers</li> | ||
Revision as of 04:49, 27 August 2014
 |
June |
 |
- Transformation with electrocompotetent E.coli KRX cells:
- K538742: kivD (parts distribution from 2013, plate 1, 24L)
- K539621: ilvC (parts distribution from 2013, plate 1, 24F)
- K539626: ilvD (parts distribution from 2013, plate 1, 22J)
- K539627: alsS (parts distribution from 2013, plate 1, 24H)
- Resulting colonies were selected to start liquid cultures:
- 5 ml of LB in 20 ml reaction tube
- incubation over night at 37°C and 250 rpm
- Plasmid isolation by ThermoScientifici MiniPrep
- Sanger sequencing using VF and VR as sequencing primers
