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Team:Bielefeld-CeBiTec/Notebook/Primer
From 2014.igem.org
(Difference between revisions)
| Line 47: | Line 47: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">sRNA:pfkB_fwd (46 bp)</td> | + | <td width="30%" id="sRNA:pfkb_fwd">sRNA:pfkB_fwd (46 bp)</td> |
<td width="70%">5‘-TACCATGCATAGATATGCAACTGTTTTCTGTTGGGCCATTGCATTG-3‘</td> | <td width="70%">5‘-TACCATGCATAGATATGCAACTGTTTTCTGTTGGGCCATTGCATTG-3‘</td> | ||
</tr> | </tr> | ||
| Line 54: | Line 54: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">VF-Primer (20 bp)</td> | + | <td width="30%" id="VF-Primer">VF-Primer (20 bp)</td> |
<td width="70%">5‘-CCACCTGACGTCTAAGAAAC-3‘</td> | <td width="70%">5‘-CCACCTGACGTCTAAGAAAC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">VR-Primer (22 bp)</td> | + | <td width="30%" id="VR-Primer">VR-Primer (22 bp)</td> |
<td width="70%">5‘-GTATTACCGCCTTTGAGTGAGC-3‘</td> | <td width="70%">5‘-GTATTACCGCCTTTGAGTGAGC-3‘</td> | ||
</tr> | </tr> | ||
| Line 67: | Line 67: | ||
<th colspan="2" align="left">Gene synthesis of the fumarate reductase of <i>B. succinogenes</i></th> | <th colspan="2" align="left">Gene synthesis of the fumarate reductase of <i>B. succinogenes</i></th> | ||
</tr> | </tr> | ||
| - | <tr | + | <tr> |
| - | <td width="30%">pSB1C3_pre_Fum_A (43 bp)</td> | + | <td width="30%" id="pSB1C3_pre_Fum_A">pSB1C3_pre_Fum_A (43 bp)</td> |
<td width="70%">5‘-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | <td width="70%">5‘-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_suf_Fum_A (43 bp)</td> | + | <td width="30%" id="pSB1C3_suf_Fum_A">pSB1C3_suf_Fum_A (43 bp)</td> |
<td width="70%">5‘-CCAAAGCGCCGAATGCGTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-CCAAAGCGCCGAATGCGTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_pre_Fum_BCD (42 bp)</td> | + | <td width="30%" id="pSB1C3_pre_Fum_BCD">pSB1C3_pre_Fum_BCD (42 bp)</td> |
<td width="70%">5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘</td> | <td width="70%">5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_suf_Fum_BCD (43 bp)</td> | + | <td width="30%" id="pSB1C3_suf_Fum_BCD">pSB1C3_suf_Fum_BCD (43 bp)</td> |
<td width="70%">5‘-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">FumBCD_fwd (24 bp)</td> | + | <td width="30%" id="FumBCD_fwd">FumBCD_fwd (24 bp)</td> |
<td width="70%">5‘-CGGCCGCTTCTAGAGAAAGAGGAG-3‘</td> | <td width="70%">5‘-CGGCCGCTTCTAGAGAAAGAGGAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">FumBCD_rev (22 bp)</td> | + | <td width="30%" id="FumBCD_rev">FumBCD_rev (22 bp)</td> |
<td width="70%">5‘-CGGCCGCTACTAGTATTAGATC-3‘</td> | <td width="70%">5‘-CGGCCGCTACTAGTATTAGATC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="FumA_seq">FumA_seq (20 bp)</td> |
<td width="70%">5‘-TGGAAGGCACCTTGGTTCAG-3‘</td> | <td width="70%">5‘-TGGAAGGCACCTTGGTTCAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">FumB_seq (20 bp)</td> | + | <td width="30%" id="FumB_seq">FumB_seq (20 bp)</td> |
<td width="70%">5‘-GCTCTGACCATGGCACATCG-3‘</td> | <td width="70%">5‘-GCTCTGACCATGGCACATCG-3‘</td> | ||
</tr> | </tr> | ||
| Line 103: | Line 103: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">ccm_fwd (58 bp)</td> | + | <td width="30%" id="ccm_fwd">ccm_fwd (58 bp)</td> |
<td width="70%">5‘-CTCAGTCCTAGGTATTGTGCAGAGAAAGAGGAGAAAATGCTTGAAGCCAGAGAGTTAC-3‘</td> | <td width="70%">5‘-CTCAGTCCTAGGTATTGTGCAGAGAAAGAGGAGAAAATGCTTGAAGCCAGAGAGTTAC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">ccm_rev (47 bp)</td> | + | <td width="30%" id="ccm_rev">ccm_rev (47 bp)</td> |
<td width="70%">5‘-TGCAGCGGCCGCTACTAGTATTATTTACTCTCCTGCGGCGACAAATG-3‘</td> | <td width="70%">5‘-TGCAGCGGCCGCTACTAGTATTATTTACTCTCCTGCGGCGACAAATG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_suf_ccm (50 bp)</td> | + | <td width="30%" id="pSB1C3_suf_ccm">pSB1C3_suf_ccm (50 bp)</td> |
<td width="70%">5‘-CATTTGTCGCCGCAGGAGAGTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-CATTTGTCGCCGCAGGAGAGTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_pre_ccm (28 bp)</td> | + | <td width="30%"id="pSB1C3_pre_ccm">pSB1C3_pre_ccm (28 bp)</td> |
<td width="70%">5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘</td> | <td width="70%">5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘</td> | ||
</tr> | </tr> | ||
| Line 122: | Line 122: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pccH_fwd (55 bp)</td> | + | <td width="30%" id="pccH_fwd">pccH_fwd (55 bp)</td> |
<td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3‘</td> | <td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pccH_rev (40 bp)</td> | + | <td width="30%" id="pccH_rev">pccH_rev (40 bp)</td> |
<td width="70%">5‘-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3‘</td> | <td width="70%">5‘-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_pre_pccH (58 bp)</td> | + | <td width="30%" id="pSB1C3_pre_pccH">pSB1C3_pre_pccH (58 bp)</td> |
<td width="70%">5‘-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | <td width="70%">5‘-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_suf_pccH (43 bp)</td> | + | <td width="30%" id="pSB1C3_suf_pccH">pSB1C3_suf_pccH (43 bp)</td> |
<td width="70%">5‘-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
| Line 141: | Line 141: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">T7_frd_Ec_fwd (64 bp)</td> | + | <td width="30%" id="T7_frd_Ec_fwd">T7_frd_Ec_fwd (64 bp)</td> |
<td width="70%">5‘-TAATACGACTCACTATAGGGAAAGAGGAGAAATACTAGATGCAAACCTTTCAAGCCGATCTTGC-3‘</td> | <td width="70%">5‘-TAATACGACTCACTATAGGGAAAGAGGAGAAATACTAGATGCAAACCTTTCAAGCCGATCTTGC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">T7_frd_Ec_rev (44 bp)</td> | + | <td width="30%" id="T7_frd_Ec_rev">T7_frd_Ec_rev (44 bp)</td> |
<td width="70%">5‘-TGCAGCGGCCGCTACTAGTATTAGATTGTAACGACACCAATCAG-3‘</td> | <td width="70%">5‘-TGCAGCGGCCGCTACTAGTATTAGATTGTAACGACACCAATCAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_frd_pre (45 bp)</td> | + | <td width="30%" id="pSB1C3_frd_pre">pSB1C3_frd_pre (45 bp)</td> |
<td width="70%">5‘-GCAAGATCGGCTTGAAAGGTTTGCATCTAGTATTTCTCCTCTTTC-3‘</td> | <td width="70%">5‘-GCAAGATCGGCTTGAAAGGTTTGCATCTAGTATTTCTCCTCTTTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_frd_suf (47 bp)</td> | + | <td width="30%" id="pSB1C3_frd_suf">pSB1C3_frd_suf (47 bp)</td> |
<td width="70%">5‘-CTGATTGGTGTCGTTACAATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-CTGATTGGTGTCGTTACAATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pR6K_dcuB_short (22 bp)</td> | + | <td width="30%" id="pR6K_dcuB_short">pR6K_dcuB_short (22 bp)</td> |
<td width="70%">5‘-AATTAACCCTCACTAAAGGGCG-3‘</td> | <td width="70%">5‘-AATTAACCCTCACTAAAGGGCG-3‘</td> | ||
</tr> | </tr> | ||
| Line 167: | Line 167: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">csoS3_can_fwd (50 bp)</td> | + | <td width="30%" id="csoS3_can_fwd">csoS3_can_fwd (50 bp)</td> |
<td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGTTAAGTAAAGTGTAACGATCATGAACACCC-3‘</td> | <td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGTTAAGTAAAGTGTAACGATCATGAACACCC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_pre_can (46 bp)</td> | + | <td width="30%" id="pSB1C3_pre_can">pSB1C3_pre_can (46 bp)</td> |
<td width="70%">5‘-GTTCATGATCGTTACACTTTACTTAACTCTAGAAGCGGCCGCGAAT-3‘</td> | <td width="70%">5‘-GTTCATGATCGTTACACTTTACTTAACTCTAGAAGCGGCCGCGAAT-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">csoS3_can_rev (41 bp)</td> | + | <td width="30%" id="csoS3_can_rev">csoS3_can_rev (41 bp)</td> |
<td width="70%">5‘-TGCAGCGGCCGCTACTAGTATCATGCGGATGCAACCTCTTC-3‘</td> | <td width="70%">5‘-TGCAGCGGCCGCTACTAGTATCATGCGGATGCAACCTCTTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_suf_can (46 bp)</td> | + | <td width="30%" id="pSB1C3_suf_can">pSB1C3_suf_can (46 bp)</td> |
<td width="70%">5‘-TCGAAGAGGTTGCATCCGCATGATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-TCGAAGAGGTTGCATCCGCATGATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
| Line 186: | Line 186: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">csoS2_sap_1_fwd (43)</td> | + | <td width="30%" id="csoS2_sap_1_fwd">csoS2_sap_1_fwd (43)</td> |
<td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGGTCAAGTCTGTCATTGCGCTGTG-3‘</td> | <td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGGTCAAGTCTGTCATTGCGCTGTG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_pre_sap_1 (43 bp)</td> | + | <td width="30%" id="pSB1C3_pre_sap_1">pSB1C3_pre_sap_1 (43 bp)</td> |
<td width="70%">5‘-AGCGCAATGACAGACTTGACCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | <td width="70%">5‘-AGCGCAATGACAGACTTGACCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">csoS2_sap_1_rev (38 bp)</td> | + | <td width="30%" id="csoS2_sap_1_rev">csoS2_sap_1_rev (38 bp)</td> |
<td width="70%">5‘-GCGCCGAACATGACTTTATCCGCTTGTGAAGGCTTTGC-3‘</td> | <td width="70%">5‘-GCGCCGAACATGACTTTATCCGCTTGTGAAGGCTTTGC-3‘</td> | ||
</tr> | </tr> | ||
| Line 201: | Line 201: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">csoS1D_fwd (40 bp)</td> | + | <td width="30%" id="csoS1D_fwd">csoS1D_fwd (40 bp)</td> |
<td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGTGTCTAGTAGGGAAGATGCG-3‘</td> | <td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGTGTCTAGTAGGGAAGATGCG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">csoS1D_rev (41 bp)</td> | + | <td width="30%" id="csoS1D_rev">csoS1D_rev (41 bp)</td> |
<td width="70%">5‘-TGCAGCGGCCGCTACTAGTATTAGAACCCTTCAGCGCGACG-3‘</td> | <td width="70%">5‘-TGCAGCGGCCGCTACTAGTATTAGAACCCTTCAGCGCGACG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_suf_csoS1D (43bp)</td> | + | <td width="30%" id="pSB1C3_suf_csoS1D">pSB1C3_suf_csoS1D (43bp)</td> |
<td width="70%">5‘-GTCGCGCTGAAGGGTTCTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-GTCGCGCTGAAGGGTTCTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_pre_csoS1D (43 bp)</td> | + | <td width="30%" id="pSB1C3_pre_csoS1D">pSB1C3_pre_csoS1D (43 bp)</td> |
<td width="70%">5‘-CGCATCTTCCCTACTAGACACTCTAGAAGCGGCCGCGAATTCC-3‘</td> | <td width="70%">5‘-CGCATCTTCCCTACTAGACACTCTAGAAGCGGCCGCGAATTCC-3‘</td> | ||
</tr> | </tr> | ||
| Line 220: | Line 220: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">fw_pSB1C3_csoS4A (47 bp)</td> | + | <td width="30%" id="fw_pSB1C3_csoS4A">fw_pSB1C3_csoS4A (47 bp)</td> |
<td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGAAATCATGCAAGTTGAGAAAACG-3‘</td> | <td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGAAATCATGCAAGTTGAGAAAACG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">rv_csoS4A_PstI (43 bp)</td> | + | <td width="30%" id="rv_csoS4A_PstI">rv_csoS4A_PstI (43 bp)</td> |
<td width="70%">5‘-GAGGGATAAGACTTGCTTCCGGCAGCCTCTCGTGCTGCCGATG-3‘</td> | <td width="70%">5‘-GAGGGATAAGACTTGCTTCCGGCAGCCTCTCGTGCTGCCGATG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">fw_PstI_csoS4A (51 bp)</td> | + | <td width="30%" id="fw_PstI_csoS4A">fw_PstI_csoS4A (51 bp)</td> |
<td width="70%">5‘-TCATCGGCAGCACGAGAGGCTGCCGGAAGCAAGTCTTATCCCTCTGATTTG-3‘</td> | <td width="70%">5‘-TCATCGGCAGCACGAGAGGCTGCCGGAAGCAAGTCTTATCCCTCTGATTTG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">rv_SpeI_Intergen (52 bp)</td> | + | <td width="30%" id="rv_SpeI_Intergen">rv_SpeI_Intergen (52 bp)</td> |
<td width="70%">5‘-GTCGTTGCCATAACAGATCCTCTAAAACTAGGTTGAGCACGTTCAGAACGAG-3‘</td> | <td width="70%">5‘-GTCGTTGCCATAACAGATCCTCTAAAACTAGGTTGAGCACGTTCAGAACGAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">fw_SpeI_Intergen (49 bp)</td> | + | <td width="30%" id="fw_SpeI_Intergen">fw_SpeI_Intergen (49 bp)</td> |
<td width="70%">5‘-TCGTTCTGAACGTGCTCAACCTAGTTTTAGAGGATCTGTTATGGCAACG-3‘</td> | <td width="70%">5‘-TCGTTCTGAACGTGCTCAACCTAGTTTTAGAGGATCTGTTATGGCAACG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id"=rv_csoS1_pSB1C3">rv_csoS1_pSB1C3 (50 bp)</td> |
<td width="70%">5‘-CGGACTGCAGCGGCCGCTACTAGTATTAGCTATTCAGATTTGCGATACAC-3‘</td> | <td width="70%">5‘-CGGACTGCAGCGGCCGCTACTAGTATTAGCTATTCAGATTTGCGATACAC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="fw_csoS1_pSB1C3">fw_csoS1_pSB1C3 (46 bp)</td> |
<td width="70%">5‘-GTGTATCGCAAATCTGAATAGCTAATACTAGTAGCGGCCGCTGCAG-3‘</td> | <td width="70%">5‘-GTGTATCGCAAATCTGAATAGCTAATACTAGTAGCGGCCGCTGCAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="rv_pSB1C3_csoS4A">rv_pSB1C3_csoS4A (47 bp)</td> |
<td width="70%">5‘-AACGTTTTCTCAACTTGCATGATTTCTCTAGAAGCGGCCGCGAATTC-3‘</td> | <td width="70%">5‘-AACGTTTTCTCAACTTGCATGATTTCTCTAGAAGCGGCCGCGAATTC-3‘</td> | ||
</tr> | </tr> | ||
| Line 255: | Line 255: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_pre_sRNApfkA (43 bp)</td> | + | <td width="30%" id="pSB1C3_pre_sRNApfkA">pSB1C3_pre_sRNApfkA (43 bp)</td> |
<td width="70%">5‘-TGTGGCTAAAAGAATTAGTACTCTAGAAGCGGCCGCGAATTCC-3‘</td> | <td width="70%">5‘-TGTGGCTAAAAGAATTAGTACTCTAGAAGCGGCCGCGAATTCC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_suf_sRNApfkA (43 bp)</td> | + | <td width="30%" id="pSB1C3_suf_sRNApfkA">pSB1C3_suf_sRNApfkA (43 bp)</td> |
<td width="70%">5‘-TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
| Line 266: | Line 266: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">int_RBS_fw (1) (42 bp)</td> | + | <td width="30%" id="int_RBS_fw">int_RBS_fw (1) (42 bp)</td> |
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATGCATCACGGGAGATGCACTAG-3‘</td> | <td width="70%">5‘-CTATAGGGAAAGAGGAGAAATGCATCACGGGAGATGCACTAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">RBS_int_rev (2) (44 bp)</td> | + | <td width="30%" id="RBS_int_rev">RBS_int_rev (2) (44 bp)</td> |
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCATTTCTCCTCTTTCCCTATAGTG-3‘</td> | <td width="70%">5‘-CTAGTGCATCTCCCGTGATGCATTTCTCCTCTTTCCCTATAGTG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">RBS_rev (3) (22 bp)</td> | + | <td width="30%" id="RBS_rev">RBS_rev (3) (22 bp)</td> |
<td width="70%">5‘-TTTCTCCTCTTTCCCTATAGTG-3‘</td> | <td width="70%">5‘-TTTCTCCTCTTTCCCTATAGTG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">int_fw (4) (22 bp)</td> | + | <td width="30%" id="int_fw">int_fw (4) (22 bp)</td> |
<td width="70%">5‘-TGCATCACGGGAGATGCACTAG-3‘</td> | <td width="70%">5‘-TGCATCACGGGAGATGCACTAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">int_pSB1C3_rev (5) (50 bp)</td> | + | <td width="30%" id="int_pSB1C3_rev">int_pSB1C3_rev (5) (50 bp)</td> |
<td width="70%">5‘-GACTGCAGCGGCCGCTACTAGTATCATTGAAGCTGCCACAAGGCAGGAAC-3‘</td> | <td width="70%">5‘-GACTGCAGCGGCCGCTACTAGTATCATTGAAGCTGCCACAAGGCAGGAAC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_int_fw (6) (46 bp)</td> | + | <td width="30%" id="pSB1C3_int_fw">pSB1C3_int_fw (6) (46 bp)</td> |
<td width="70%">5‘-CTGCCTTGTGGCAGCTTCAATGATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-CTGCCTTGTGGCAGCTTCAATGATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
| Line 293: | Line 293: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">SBPase_purify_fw (45 bp)</td> | + | <td width="30%" id="SBPase_purify_fw">SBPase_purify_fw (45 bp)</td> |
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGAGGGAATTGAAAAGCGAAAAG-3‘</td> | <td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGAGGGAATTGAAAAGCGAAAAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">SBPase_purify_rev (53 bp)</td> | + | <td width="30%" id="SBPase_purfiy_rev">SBPase_purify_rev (53 bp)</td> |
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACGTAAAATACCAATTTATTGAAGCTTATCAT-3‘</td> | <td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACGTAAAATACCAATTTATTGAAGCTTATCAT-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">fw_pSB1_SBPase (58 bp)</td> | + | <td width="30%" id="fw_pSB1_SBPase">fw_pSB1_SBPase (58 bp)</td> |
<td width="70%">5‘-GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGAGGGAATTGAAAAGCGAAAAG-3‘</td> | <td width="70%">5‘-GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGAGGGAATTGAAAAGCGAAAAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">rv_pSB1_SBPase (56 bp)</td> | + | <td width="30%" id="rv_pSB1_SBPase">rv_pSB1_SBPase (56 bp)</td> |
<td width="70%">5‘-GACTGCAGCGGCCGCTACTAGTATCAATGATAAGCTTCAATAAATTGGTATTTTAC-3‘</td> | <td width="70%">5‘-GACTGCAGCGGCCGCTACTAGTATCAATGATAAGCTTCAATAAATTGGTATTTTAC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">fw_SBPase_XbaI (56 bp)</td> | + | <td width="30%" id="fw_SBPase_XbaI">fw_SBPase_XbaI (56 bp)</td> |
<td width="70%">5‘-AAAAGCAAAAGGCTGCGTAAATTTGGACGCATCTTTAACAGAAAATATGAAATCAG-3‘</td> | <td width="70%">5‘-AAAAGCAAAAGGCTGCGTAAATTTGGACGCATCTTTAACAGAAAATATGAAATCAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">rv_SBPase_XbaI (46 bp)</td> | + | <td width="30%" id="rv_SBPase_XbaI">rv_SBPase_XbaI (46 bp)</td> |
<td width="70%">5‘-TTTTCTGTTAAAGATGCGTCCAAATTTACGCAGCCTTTTGCTTTTG-3‘</td> | <td width="70%">5‘-TTTTCTGTTAAAGATGCGTCCAAATTTACGCAGCCTTTTGCTTTTG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">fw_SBPase_pSB1C3 (57 bp)</td> | + | <td width="30%" id="fw_SBPase_pSB1C3">fw_SBPase_pSB1C3 (57 bp)</td> |
<td width="70%">5‘-AATTTATTGAAGCTTATCATTGATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG-3‘</td> | <td width="70%">5‘-AATTTATTGAAGCTTATCATTGATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">rv_SBPase_pSB1 (65 bp)</td> | + | <td width="30%" id="rv_SBPase_pSB1">rv_SBPase_pSB1 (65 bp)</td> |
<td width="70%">5‘-TTTTCGCTTTTCAATTCCCTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCCAGAAATC-3‘</td> | <td width="70%">5‘-TTTTCGCTTTTCAATTCCCTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCCAGAAATC-3‘</td> | ||
</tr> | </tr> | ||
| Line 328: | Line 328: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">fbaA_purify_fw (50 bp)</td> | + | <td width="30%" id="fbaA_purify_fw">fbaA_purify_fw (50 bp)</td> |
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGTCTAAGATTTTTGATTTCGTAAAACC-3‘</td> | <td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGTCTAAGATTTTTGATTTCGTAAAACC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">fbaA_purify_rev (41 bp)</td> | + | <td width="30%" id="fbaA_purify_rev">fbaA_purify_rev (41 bp)</td> |
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACAGAACGTCGATCGCGTTC-3‘</td> | <td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACAGAACGTCGATCGCGTTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">fbaA_PstI_fw (42 bp)</td> | + | <td width="30%" id="fbaA_PstI_fw">fbaA_PstI_fw (42 bp)</td> |
<td width="70%">5‘-CAAAGCGAACGAAGCTTATCTGCAAGGTCAGCTGGGTAACCC-3‘</td> | <td width="70%">5‘-CAAAGCGAACGAAGCTTATCTGCAAGGTCAGCTGGGTAACCC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">fbaA_PstI_rev (43 bp)</td> | + | <td width="30%" id="fbaA_PstI_rev">fbaA_PstI_rev (43 bp)</td> |
<td width="70%">5‘-CTTTCGGGTTACCCAGCTGACCTTGCAGATAAGCTTCGTTCGC-3‘</td> | <td width="70%">5‘-CTTTCGGGTTACCCAGCTGACCTTGCAGATAAGCTTCGTTCGC-3‘</td> | ||
</tr> | </tr> | ||
| Line 347: | Line 347: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">tktA_purify_fw (43 bp)</td> | + | <td width="30%" id="tktA_purify_fw">tktA_purify_fw (43 bp)</td> |
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGTCCTCACGTAAAGAGCTTG-3‘</td> | <td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGTCCTCACGTAAAGAGCTTG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">tktA_purify_rev (43 bp)</td> | + | <td width="30%" id="tktA_purify_rev">tktA_purify_rev (43 bp)</td> |
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACAGCAGTTCTTTTGCTTTCGC-3‘</td> | <td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACAGCAGTTCTTTTGCTTTCGC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">tktA_PstI_fw (41 bp)</td> | + | <td width="30%" id="tktA_PstI_fw">tktA_PstI_fw (41 bp)</td> |
<td width="70%">5‘-GTTCGGTCCGCTGTTGCCGGAACTCCTCGGCGGTTCTGCTG-3‘</td> | <td width="70%">5‘-GTTCGGTCCGCTGTTGCCGGAACTCCTCGGCGGTTCTGCTG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">tktA_PstI_rev (42 bp)</td> | + | <td width="30%" id="tktA_PstI_rev">tktA_PstI_rev (42 bp)</td> |
<td width="70%">5‘-CAGGTCAGCAGAACCGCCGAGGAGTTCCGGCAACAGCGGACC-3‘</td> | <td width="70%">5‘-CAGGTCAGCAGAACCGCCGAGGAGTTCCGGCAACAGCGGACC-3‘</td> | ||
</tr> | </tr> | ||
| Line 366: | Line 366: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">cg_pgk_purify_fw (42 bp)</td> | + | <td width="30%" id="cg_pgk_purify_fw">cg_pgk_purify_fw (42 bp)</td> |
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGGCTGTTAAGACCCTCAAG-3‘</td> | <td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGGCTGTTAAGACCCTCAAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">cg_pgk_purify_rev (42 bp)</td> | + | <td width="30%" id="cg_pgk_purify_rev">cg_pgk_purify_rev (42 bp)</td> |
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACTGAGCGAGAATTGCAACGC-3‘</td> | <td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACTGAGCGAGAATTGCAACGC-3‘</td> | ||
</tr> | </tr> | ||
| Line 377: | Line 377: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">gapA_purify_fw (47 bp)</td> | + | <td width="30%" id="gapA_purify_fw">gapA_purify_fw (47 bp)</td> |
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGACTATCAAAGTAGGTATCAACGG-3‘</td> | <td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGACTATCAAAGTAGGTATCAACGG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">gapA_purify_rev (43 bp)</td> | + | <td width="30%" id="gapA_purify_rev">gapA_purify_rev (43 bp)</td> |
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCATTTGGAGATGTGAGCGATCAG-3‘</td> | <td width="70%">5‘-CTAGTGCATCTCCCGTGATGCATTTGGAGATGTGAGCGATCAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">gapA_NotI_fw (44 bp)</td> | + | <td width="30%" id="gapA_NotI_fw">gapA_NotI_fw (44 bp)</td> |
<td width="70%">5‘-CTCACAAAGACTGGCGCGGCGGCCGTGGCGCTTCCCAGAACATC-3‘</td> | <td width="70%">5‘-CTCACAAAGACTGGCGCGGCGGCCGTGGCGCTTCCCAGAACATC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">gapA_NotI_rev (43 bp)</td> | + | <td width="30%" id="gapA_NotI_rev">gapA_NotI_rev (43 bp)</td> |
<td width="70%">5‘-GATGATGTTCTGGGAAGCGCCACGGCCGCCGCGCCAGTCTTTG-3‘</td> | <td width="70%">5‘-GATGATGTTCTGGGAAGCGCCACGGCCGCCGCGCCAGTCTTTG-3‘</td> | ||
</tr> | </tr> | ||
| Line 396: | Line 396: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">prkA_purify_fw (40 bp)</td> | + | <td width="30%" id="prkA_purify_fw">prkA_purify_fw (40 bp)</td> |
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGAGCAAGCCAGATCGTG-3‘</td> | <td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGAGCAAGCCAGATCGTG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">prkA_purify_rev (42 bp)</td> | + | <td width="30%" id="prkA_purify_rev">prkA_purify_rev (42 bp)</td> |
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCAAACGCTAGCGGCAACCGGTG-3‘</td> | <td width="70%">5‘-CTAGTGCATCTCCCGTGATGCAAACGCTAGCGGCAACCGGTG-3‘</td> | ||
</tr> | </tr> | ||
| Line 407: | Line 407: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pykF_purify_fw (47 bp)</td> | + | <td width="30%" id="pykF_purify_fw">pykF_purify_fw (47 bp)</td> |
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGAAAAAGACCAAAATTGTTTGCAC-3‘</td> | <td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGAAAAAGACCAAAATTGTTTGCAC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pykF_purify_rev (42 bp)</td> | + | <td width="30%" id="pykF_purify_rev">pykF_purify_rev (42 bp)</td> |
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACAGGACGTGAACAGATGCGG-3‘</td> | <td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACAGGACGTGAACAGATGCGG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pykF_PstI_fw (48 bp)</td> | + | <td width="30%" id="pykF_PstI_fw">pykF_PstI_fw (48 bp)</td> |
<td width="70%">5‘-ACCGTCTGGGTAAAGAACTGGCTCTGCAAAGCGGTCTGGCACACAAAG-3‘</td> | <td width="70%">5‘-ACCGTCTGGGTAAAGAACTGGCTCTGCAAAGCGGTCTGGCACACAAAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pykF_PstI_rev (43 bp)</td> | + | <td width="30%" id="pykF_PstI_rev">pykF_PstI_rev (43 bp)</td> |
<td width="70%">5‘-CACCTTTGTGTGCCAGACCGCTTTGCAGAGCCAGTTCTTTACC-3‘</td> | <td width="70%">5‘-CACCTTTGTGTGCCAGACCGCTTTGCAGAGCCAGTTCTTTACC-3‘</td> | ||
</tr> | </tr> | ||
| Line 426: | Line 426: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">ldhA_purify_fw (44 bp)</td> | + | <td width="30%" id="ldhA_purify_fw">ldhA_purify_fw (44 bp)</td> |
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGAAACTCGCCGTTTATAGCAC-3‘</td> | <td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGAAACTCGCCGTTTATAGCAC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">ldhA_purify_rev (42 bp)</td> | + | <td width="30%" id="ldhA_purify_rev">ldhA_purify_rev (42 bp)</td> |
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCAAACCAGTTCGTTCGGGCAGG-3‘</td> | <td width="70%">5‘-CTAGTGCATCTCCCGTGATGCAAACCAGTTCGTTCGGGCAGG-3‘</td> | ||
</tr> | </tr> | ||
| Line 437: | Line 437: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">glk_purify_fw (44 bp)</td> | + | <td width="30%" id="glk_purify_fw">glk_purify_fw (44 bp)</td> |
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGACAAAGTATGCATTAGTCGG-3‘</td> | <td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGACAAAGTATGCATTAGTCGG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">glk_purify_rev (43 bp)</td> | + | <td width="30%" id="glk_purify_rev">glk_purify_rev (43 bp)</td> |
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACAGAATGTGACCTAAGGTCTG-3‘</td> | <td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACAGAATGTGACCTAAGGTCTG-3‘</td> | ||
</tr> | </tr> | ||
| Line 448: | Line 448: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">zwf_purify_fw (41 bp)</td> | + | <td width="30%" id="zwf_purify_fw">zwf_purify_fw (41 bp)</td> |
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGGCGGTAACGCAAACAGC-3‘</td> | <td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGGCGGTAACGCAAACAGC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">zwf_purify_rev (45 bp)</td> | + | <td width="30%" id="zwf_purify_rev">zwf_purify_rev (45 bp)</td> |
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACTCAAACTCATTCCAGGAACGAC-3‘</td> | <td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACTCAAACTCATTCCAGGAACGAC-3‘</td> | ||
</tr> | </tr> | ||
| Line 459: | Line 459: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_pre_rbcLS (44 bp)</td> | + | <td width="30%" id="pSB1C3_pre_rbcLS">pSB1C3_pre_rbcLS (44 bp)</td> |
<td width="70%">5‘-CATCTAGTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | <td width="70%">5‘-CATCTAGTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_suf_rbcLS (43 bp)</td> | + | <td width="30%" id="pSB1C3_suf_rbcLS">pSB1C3_suf_rbcLS (43 bp)</td> |
<td width="70%">5‘-ATCGTCCGGGCCGCTACTAA-TACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-ATCGTCCGGGCCGCTACTAA-TACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_pre_ccbLS (44 bp)</td> | + | <td width="30%" id="pSB1C3_pre_ccbLS">pSB1C3_pre_ccbLS (44 bp)</td> |
<td width="70%">5‘-CATCTAGTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | <td width="70%">5‘-CATCTAGTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_suf_ccbLS (43 bp)</td> | + | <td width="30%" id="pSB1C3_suf_ccbLS">pSB1C3_suf_ccbLS (43 bp)</td> |
<td width="70%">5‘-TGGTTTACCGCGGCAACTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-TGGTTTACCGCGGCAACTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_pre_prkA (43 bp)</td> | + | <td width="30%" id="pSB1C3_pre_prkA">pSB1C3_pre_prkA (43 bp)</td> |
<td width="70%">5‘-ACACGATCTGGCTTGCTCATCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | <td width="70%">5‘-ACACGATCTGGCTTGCTCATCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">pSB1C3_suf_prkA (43 bp)</td> | + | <td width="30%" id="pSB1C3_suf_prkA">pSB1C3_suf_prkA (43 bp)</td> |
<td width="70%">5‘-CGGTTGCCGCTAGCGTTTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-CGGTTGCCGCTAGCGTTTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
| Line 489: | Line 489: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="fw_kivD_pSB1C3">fw_kivD_pSB1C3 (53 bp)</td> |
<td width="70%">5‘-GCAAACTATTTGCTGAACAAAATAAATCATAATACTAGTAGCGGCCGCTGCAG-3‘</td> | <td width="70%">5‘-GCAAACTATTTGCTGAACAAAATAAATCATAATACTAGTAGCGGCCGCTGCAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="rv_alsS_pSB1C3">rv_alsS_pSB1C3 (65 bp)</td> |
<td width="70%">5‘-TTTTTGTTCTTTTGTTGCTTTTGTCAACATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTC-3‘</td> | <td width="70%">5‘-TTTTTGTTCTTTTGTTGCTTTTGTCAACATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">fw_adhA_pSB1C3 (51 bp)</td> | + | <td width="30%" id="fw_adhA_pSB1C3">fw_adhA_pSB1C3 (51 bp)</td> |
<td width="70%">5‘-GGCCGAATGGTCATTGATTTTACTAAATAATACTAGTAGCGGCCGCTGCAG-3‘</td> | <td width="70%">5‘-GGCCGAATGGTCATTGATTTTACTAAATAATACTAGTAGCGGCCGCTGCAG-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="fw_adhA_pSB1C3">fw_adhA_pSB1C3 (43 bp)</td> |
<td width="70%">5‘-CATTGATTTTACTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | <td width="70%">5‘-CATTGATTTTACTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
| Line 508: | Line 508: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%"id="fw_pSB1C3_alsS">fw_pSB1C3_alsS (79 bp)</td> |
<td width="70%">5‘-TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGTTGACAAAAGCAACAAAAGAAC-3‘</td> | <td width="70%">5‘-TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGTTGACAAAAGCAACAAAAGAAC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="rv_ilvC_alsS">rv_ilvC_alsS (64 bp)</td> |
<td width="70%">5‘-GATTCAGTGTATTGAAGTAGTTAGCCATTTTCTCCTCTTTCTAGAGAGCTTTCGTTTTCATGAG-3‘</td> | <td width="70%">5‘-GATTCAGTGTATTGAAGTAGTTAGCCATTTTCTCCTCTTTCTAGAGAGCTTTCGTTTTCATGAG-3‘</td> | ||
</tr> | </tr> | ||
| Line 519: | Line 519: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="fw_alsS_ilvC">fw_alsS_ilvC (65 bp)</td> |
<td width="70%">5‘-CTCATGAAAACGAAAGCTCTCTAGAAAGAGGAGAAAATGGCTAACTACTTCAATACACTGAATC-3‘</td> | <td width="70%">5‘-CTCATGAAAACGAAAGCTCTCTAGAAAGAGGAGAAAATGGCTAACTACTTCAATACACTGAATC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="rev_ilvD_ilvC">rev_ilvD_ilvC (56 bp)</td> |
<td width="70%">5‘-GTGGCGGAACGGTACTTAGGCATTTTCTCCTCTTTTTAACCCGCAACAGCAATACG-3‘</td> | <td width="70%">5‘-GTGGCGGAACGGTACTTAGGCATTTTCTCCTCTTTTTAACCCGCAACAGCAATACG-3‘</td> | ||
</tr> | </tr> | ||
| Line 530: | Line 530: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="fw_ilvC_ilvD">fw_ilvC_ilvD (57 bp)</td> |
<td width="70%">5‘-CGTATTGCTGTTGCGGGTTAAAAAGAGGAGAAAATGCCTAAGTACCGTTCCGCCAC-3‘</td> | <td width="70%">5‘-CGTATTGCTGTTGCGGGTTAAAAAGAGGAGAAAATGCCTAAGTACCGTTCCGCCAC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="rv_kivD_ilvD">rv_kivD_ilvD (67 bp)</td> |
<td width="70%">5‘-GTCTAATAGGTAATCTCCTACTGTATACATTTTCTCCTCTTTTTAACCCCCCAGTTTCGATTTATC-3‘</td> | <td width="70%">5‘-GTCTAATAGGTAATCTCCTACTGTATACATTTTCTCCTCTTTTTAACCCCCCAGTTTCGATTTATC-3‘</td> | ||
</tr> | </tr> | ||
| Line 541: | Line 541: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="fw_ilvD_kivD">fw_ilvD_kivD (67 bp)</td> |
<td width="70%">5‘-GATAAATCGAAACTGGGGGGTTAAAAAGAGGAGAAAATGTATACAGTAGGAGATTACCTATTAGAC-3‘</td> | <td width="70%">5‘-GATAAATCGAAACTGGGGGGTTAAAAAGAGGAGAAAATGTATACAGTAGGAGATTACCTATTAGAC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="rv_pSB1C3_kivD">rv_pSB1C3_kivD (74 bp)</td> |
<td width="70%">5‘-ACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATGATTTATTTTGTTCAGCAAATAGTTTGC-3‘</td> | <td width="70%">5‘-ACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATGATTTATTTTGTTCAGCAAATAGTTTGC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="rv_adhA_kivD">rv_adhA_kivD (69 bp)</td> |
<td width="70%">5‘-GTGTCTTACTACTGCTGCTTTCATTTTCTCCTCTTTTTATGATTTATTTTGTTCAGCAAATAGTTTGC-3‘</td> | <td width="70%">5‘-GTGTCTTACTACTGCTGCTTTCATTTTCTCCTCTTTTTATGATTTATTTTGTTCAGCAAATAGTTTGC-3‘</td> | ||
</tr> | </tr> | ||
| Line 556: | Line 556: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="fw_kivD_adhA">fw_kivD_adhA (69 bp)</td> |
<td width="70%">5‘-GCAAACTATTTGCTGAACAAAATAAATCATAAAAAGAGGAGAAAATGAAAGCAGCAGTAGTAAGACAC-3‘</td> | <td width="70%">5‘-GCAAACTATTTGCTGAACAAAATAAATCATAAAAAGAGGAGAAAATGAAAGCAGCAGTAGTAAGACAC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%"> | + | <td width="30%" id="rev_pSB1C3_adhA">rev_pSB1C3_adhA (80 bp)</td> |
<td width="70%">5‘-GTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTAGTAAAATCAATGACCATTCGGCCTTC-3‘</td> | <td width="70%">5‘-GTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTAGTAAAATCAATGACCATTCGGCCTTC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">fw_pSB1C3_adhA (66 bp)</td> | + | <td width="30%" id="fw_pSB1C3_adhA">fw_pSB1C3_adhA (66 bp)</td> |
<td width="70%">5‘-GATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGAAAGCAGCAGTAGTAAGACAC-3‘</td> | <td width="70%">5‘-GATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGAAAGCAGCAGTAGTAAGACAC-3‘</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="30%">rev_adhA_pSB1C3 (56 bp)</td> | + | <td width="30%" id="rev_adhA_pSB1C3">rev_adhA_pSB1C3 (56 bp)</td> |
<td width="70%">5‘-GTCTTACTACTGCTGCTTTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTC-3‘</td> | <td width="70%">5‘-GTCTTACTACTGCTGCTTTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTC-3‘</td> | ||
</tr> | </tr> | ||
Revision as of 07:53, 26 August 2014
Primerlist
| Primer ID | Primer sequence |
|---|---|
| ????????? | |
| sRNA:pfkB_fwd (46 bp) | 5‘-TACCATGCATAGATATGCAACTGTTTTCTGTTGGGCCATTGCATTG-3‘ |
| General | |
| VF-Primer (20 bp) | 5‘-CCACCTGACGTCTAAGAAAC-3‘ |
| VR-Primer (22 bp) | 5‘-GTATTACCGCCTTTGAGTGAGC-3‘ |
| Module 1 | |
| Gene synthesis of the fumarate reductase of B. succinogenes | |
| pSB1C3_pre_Fum_A (43 bp) | 5‘-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_Fum_A (43 bp) | 5‘-CCAAAGCGCCGAATGCGTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_Fum_BCD (42 bp) | 5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘ |
| pSB1C3_suf_Fum_BCD (43 bp) | 5‘-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| FumBCD_fwd (24 bp) | 5‘-CGGCCGCTTCTAGAGAAAGAGGAG-3‘ |
| FumBCD_rev (22 bp) | 5‘-CGGCCGCTACTAGTATTAGATC-3‘ |
| FumA_seq (20 bp) | 5‘-TGGAAGGCACCTTGGTTCAG-3‘ |
| FumB_seq (20 bp) | 5‘-GCTCTGACCATGGCACATCG-3‘ |
| CCM-Cassette (E. coli) | |
| ccm_fwd (58 bp) | 5‘-CTCAGTCCTAGGTATTGTGCAGAGAAAGAGGAGAAAATGCTTGAAGCCAGAGAGTTAC-3‘ |
| ccm_rev (47 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTATTTACTCTCCTGCGGCGACAAATG-3‘ |
| pSB1C3_suf_ccm (50 bp) | 5‘-CATTTGTCGCCGCAGGAGAGTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_ccm (28 bp) | 5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘ |
| Cytochrom C (Geobacter sulfurreducens) | |
| pccH_fwd (55 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3‘ |
| pccH_rev (40 bp) | 5‘-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3‘ |
| pSB1C3_pre_pccH (58 bp) | 5‘-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_pccH (43 bp) | 5‘-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| ??? (Julian) | |
| T7_frd_Ec_fwd (64 bp) | 5‘-TAATACGACTCACTATAGGGAAAGAGGAGAAATACTAGATGCAAACCTTTCAAGCCGATCTTGC-3‘ |
| T7_frd_Ec_rev (44 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTAGATTGTAACGACACCAATCAG-3‘ |
| pSB1C3_frd_pre (45 bp) | 5‘-GCAAGATCGGCTTGAAAGGTTTGCATCTAGTATTTCTCCTCTTTC-3‘ |
| pSB1C3_frd_suf (47 bp) | 5‘-CTGATTGGTGTCGTTACAATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pR6K_dcuB_short (22 bp) | 5‘-AATTAACCCTCACTAAAGGGCG-3‘ |
| Module 2 | |
| Carbonic anhydrase | |
| csoS3_can_fwd (50 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGTTAAGTAAAGTGTAACGATCATGAACACCC-3‘ |
| pSB1C3_pre_can (46 bp) | 5‘-GTTCATGATCGTTACACTTTACTTAACTCTAGAAGCGGCCGCGAAT-3‘ |
| csoS3_can_rev (41 bp) | 5‘-TGCAGCGGCCGCTACTAGTATCATGCGGATGCAACCTCTTC-3‘ |
| pSB1C3_suf_can (46 bp) | 5‘-TCGAAGAGGTTGCATCCGCATGATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| Shell associated protein | |
| csoS2_sap_1_fwd (43) | 5‘-ATTCGCGGCCGCTTCTAGAGGTCAAGTCTGTCATTGCGCTGTG-3‘ |
| pSB1C3_pre_sap_1 (43 bp) | 5‘-AGCGCAATGACAGACTTGACCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| csoS2_sap_1_rev (38 bp) | 5‘-GCGCCGAACATGACTTTATCCGCTTGTGAAGGCTTTGC-3‘ |
| csoS1D | |
| csoS1D_fwd (40 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGTGTCTAGTAGGGAAGATGCG-3‘ |
| csoS1D_rev (41 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTAGAACCCTTCAGCGCGACG-3‘ |
| pSB1C3_suf_csoS1D (43bp) | 5‘-GTCGCGCTGAAGGGTTCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_csoS1D (43 bp) | 5‘-CGCATCTTCCCTACTAGACACTCTAGAAGCGGCCGCGAATTCC-3‘ |
| Second part | |
| fw_pSB1C3_csoS4A (47 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGAAATCATGCAAGTTGAGAAAACG-3‘ |
| rv_csoS4A_PstI (43 bp) | 5‘-GAGGGATAAGACTTGCTTCCGGCAGCCTCTCGTGCTGCCGATG-3‘ |
| fw_PstI_csoS4A (51 bp) | 5‘-TCATCGGCAGCACGAGAGGCTGCCGGAAGCAAGTCTTATCCCTCTGATTTG-3‘ |
| rv_SpeI_Intergen (52 bp) | 5‘-GTCGTTGCCATAACAGATCCTCTAAAACTAGGTTGAGCACGTTCAGAACGAG-3‘ |
| fw_SpeI_Intergen (49 bp) | 5‘-TCGTTCTGAACGTGCTCAACCTAGTTTTAGAGGATCTGTTATGGCAACG-3‘ |
| rv_csoS1_pSB1C3 (50 bp) | 5‘-CGGACTGCAGCGGCCGCTACTAGTATTAGCTATTCAGATTTGCGATACAC-3‘ |
| fw_csoS1_pSB1C3 (46 bp) | 5‘-GTGTATCGCAAATCTGAATAGCTAATACTAGTAGCGGCCGCTGCAG-3‘ |
| rv_pSB1C3_csoS4A (47 bp) | 5‘-AACGTTTTCTCAACTTGCATGATTTCTCTAGAAGCGGCCGCGAATTC-3‘ |
| Gluco switch | |
| pSB1C3_pre_sRNApfkA (43 bp) | 5‘-TGTGGCTAAAAGAATTAGTACTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_sRNApfkA (43 bp) | 5‘-TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| General primer | |
| int_RBS_fw (1) (42 bp) | 5‘-CTATAGGGAAAGAGGAGAAATGCATCACGGGAGATGCACTAG-3‘ |
| RBS_int_rev (2) (44 bp) | 5‘-CTAGTGCATCTCCCGTGATGCATTTCTCCTCTTTCCCTATAGTG-3‘ |
| RBS_rev (3) (22 bp) | 5‘-TTTCTCCTCTTTCCCTATAGTG-3‘ |
| int_fw (4) (22 bp) | 5‘-TGCATCACGGGAGATGCACTAG-3‘ |
| int_pSB1C3_rev (5) (50 bp) | 5‘-GACTGCAGCGGCCGCTACTAGTATCATTGAAGCTGCCACAAGGCAGGAAC-3‘ |
| pSB1C3_int_fw (6) (46 bp) | 5‘-CTGCCTTGTGGCAGCTTCAATGATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| SBPase (glpX) | |
| SBPase_purify_fw (45 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGAGGGAATTGAAAAGCGAAAAG-3‘ |
| SBPase_purify_rev (53 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACGTAAAATACCAATTTATTGAAGCTTATCAT-3‘ |
| fw_pSB1_SBPase (58 bp) | 5‘-GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGAGGGAATTGAAAAGCGAAAAG-3‘ |
| rv_pSB1_SBPase (56 bp) | 5‘-GACTGCAGCGGCCGCTACTAGTATCAATGATAAGCTTCAATAAATTGGTATTTTAC-3‘ |
| fw_SBPase_XbaI (56 bp) | 5‘-AAAAGCAAAAGGCTGCGTAAATTTGGACGCATCTTTAACAGAAAATATGAAATCAG-3‘ |
| rv_SBPase_XbaI (46 bp) | 5‘-TTTTCTGTTAAAGATGCGTCCAAATTTACGCAGCCTTTTGCTTTTG-3‘ |
| fw_SBPase_pSB1C3 (57 bp) | 5‘-AATTTATTGAAGCTTATCATTGATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG-3‘ |
| rv_SBPase_pSB1 (65 bp) | 5‘-TTTTCGCTTTTCAATTCCCTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCCAGAAATC-3‘ |
| fbaA | |
| fbaA_purify_fw (50 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGTCTAAGATTTTTGATTTCGTAAAACC-3‘ |
| fbaA_purify_rev (41 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACAGAACGTCGATCGCGTTC-3‘ |
| fbaA_PstI_fw (42 bp) | 5‘-CAAAGCGAACGAAGCTTATCTGCAAGGTCAGCTGGGTAACCC-3‘ |
| fbaA_PstI_rev (43 bp) | 5‘-CTTTCGGGTTACCCAGCTGACCTTGCAGATAAGCTTCGTTCGC-3‘ |
| tktA | |
| tktA_purify_fw (43 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGTCCTCACGTAAAGAGCTTG-3‘ |
| tktA_purify_rev (43 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACAGCAGTTCTTTTGCTTTCGC-3‘ |
| tktA_PstI_fw (41 bp) | 5‘-GTTCGGTCCGCTGTTGCCGGAACTCCTCGGCGGTTCTGCTG-3‘ |
| tktA_PstI_rev (42 bp) | 5‘-CAGGTCAGCAGAACCGCCGAGGAGTTCCGGCAACAGCGGACC-3‘ |
| cg_pgk | |
| cg_pgk_purify_fw (42 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGGCTGTTAAGACCCTCAAG-3‘ |
| cg_pgk_purify_rev (42 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACTGAGCGAGAATTGCAACGC-3‘ |
| gapA | |
| gapA_purify_fw (47 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGACTATCAAAGTAGGTATCAACGG-3‘ |
| gapA_purify_rev (43 bp) | 5‘-CTAGTGCATCTCCCGTGATGCATTTGGAGATGTGAGCGATCAG-3‘ |
| gapA_NotI_fw (44 bp) | 5‘-CTCACAAAGACTGGCGCGGCGGCCGTGGCGCTTCCCAGAACATC-3‘ |
| gapA_NotI_rev (43 bp) | 5‘-GATGATGTTCTGGGAAGCGCCACGGCCGCCGCGCCAGTCTTTG-3‘ |
| prkA | |
| prkA_purify_fw (40 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGAGCAAGCCAGATCGTG-3‘ |
| prkA_purify_rev (42 bp) | 5‘-CTAGTGCATCTCCCGTGATGCAAACGCTAGCGGCAACCGGTG-3‘ |
| pykF | |
| pykF_purify_fw (47 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGAAAAAGACCAAAATTGTTTGCAC-3‘ |
| pykF_purify_rev (42 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACAGGACGTGAACAGATGCGG-3‘ |
| pykF_PstI_fw (48 bp) | 5‘-ACCGTCTGGGTAAAGAACTGGCTCTGCAAAGCGGTCTGGCACACAAAG-3‘ |
| pykF_PstI_rev (43 bp) | 5‘-CACCTTTGTGTGCCAGACCGCTTTGCAGAGCCAGTTCTTTACC-3‘ |
| ldhA | |
| ldhA_purify_fw (44 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGAAACTCGCCGTTTATAGCAC-3‘ |
| ldhA_purify_rev (42 bp) | 5‘-CTAGTGCATCTCCCGTGATGCAAACCAGTTCGTTCGGGCAGG-3‘ |
| glk | |
| glk_purify_fw (44 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGACAAAGTATGCATTAGTCGG-3‘ |
| glk_purify_rev (43 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACAGAATGTGACCTAAGGTCTG-3‘ |
| zwf | |
| zwf_purify_fw (41 bp) | 5‘-CTATAGGGAAAGAGGAGAAATATGGCGGTAACGCAAACAGC-3‘ |
| zwf_purify_rev (45 bp) | 5‘-CTAGTGCATCTCCCGTGATGCACTCAAACTCATTCCAGGAACGAC-3‘ |
| Gene synthesis | |
| pSB1C3_pre_rbcLS (44 bp) | 5‘-CATCTAGTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_rbcLS (43 bp) | 5‘-ATCGTCCGGGCCGCTACTAA-TACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_ccbLS (44 bp) | 5‘-CATCTAGTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_ccbLS (43 bp) | 5‘-TGGTTTACCGCGGCAACTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_prkA (43 bp) | 5‘-ACACGATCTGGCTTGCTCATCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_prkA (43 bp) | 5‘-CGGTTGCCGCTAGCGTTTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| Module 3 | |
| Backbone | |
| fw_kivD_pSB1C3 (53 bp) | 5‘-GCAAACTATTTGCTGAACAAAATAAATCATAATACTAGTAGCGGCCGCTGCAG-3‘ |
| rv_alsS_pSB1C3 (65 bp) | 5‘-TTTTTGTTCTTTTGTTGCTTTTGTCAACATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTC-3‘ |
| fw_adhA_pSB1C3 (51 bp) | 5‘-GGCCGAATGGTCATTGATTTTACTAAATAATACTAGTAGCGGCCGCTGCAG-3‘ |
| fw_adhA_pSB1C3 (43 bp) | 5‘-CATTGATTTTACTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| alsS | |
| fw_pSB1C3_alsS (79 bp) | 5‘-TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGTTGACAAAAGCAACAAAAGAAC-3‘ |
| rv_ilvC_alsS (64 bp) | 5‘-GATTCAGTGTATTGAAGTAGTTAGCCATTTTCTCCTCTTTCTAGAGAGCTTTCGTTTTCATGAG-3‘ |
| ilvC | |
| fw_alsS_ilvC (65 bp) | 5‘-CTCATGAAAACGAAAGCTCTCTAGAAAGAGGAGAAAATGGCTAACTACTTCAATACACTGAATC-3‘ |
| rev_ilvD_ilvC (56 bp) | 5‘-GTGGCGGAACGGTACTTAGGCATTTTCTCCTCTTTTTAACCCGCAACAGCAATACG-3‘ |
| ilvD | |
| fw_ilvC_ilvD (57 bp) | 5‘-CGTATTGCTGTTGCGGGTTAAAAAGAGGAGAAAATGCCTAAGTACCGTTCCGCCAC-3‘ |
| rv_kivD_ilvD (67 bp) | 5‘-GTCTAATAGGTAATCTCCTACTGTATACATTTTCTCCTCTTTTTAACCCCCCAGTTTCGATTTATC-3‘ |
| kivD | |
| fw_ilvD_kivD (67 bp) | 5‘-GATAAATCGAAACTGGGGGGTTAAAAAGAGGAGAAAATGTATACAGTAGGAGATTACCTATTAGAC-3‘ |
| rv_pSB1C3_kivD (74 bp) | 5‘-ACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATGATTTATTTTGTTCAGCAAATAGTTTGC-3‘ |
| rv_adhA_kivD (69 bp) | 5‘-GTGTCTTACTACTGCTGCTTTCATTTTCTCCTCTTTTTATGATTTATTTTGTTCAGCAAATAGTTTGC-3‘ |
| adhA | |
| fw_kivD_adhA (69 bp) | 5‘-GCAAACTATTTGCTGAACAAAATAAATCATAAAAAGAGGAGAAAATGAAAGCAGCAGTAGTAAGACAC-3‘ |
| rev_pSB1C3_adhA (80 bp) | 5‘-GTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTAGTAAAATCAATGACCATTCGGCCTTC-3‘ |
| fw_pSB1C3_adhA (66 bp) | 5‘-GATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGAAAGCAGCAGTAGTAAGACAC-3‘ |
| rev_adhA_pSB1C3 (56 bp) | 5‘-GTCTTACTACTGCTGCTTTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTC-3‘ |
| Biosafety | |
