Team:TU Eindhoven/Notebook

From 2014.igem.org

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       <div class="slider">
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         <ul>
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          <li class="category active"><a href="#notebook">NOTEBOOK</a></li>
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<li class="category active"><a href="#january">JANUARY</a></li>
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<li class="category"><a href="#january">JANUARY</a></li>
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           <li class="category"><a href="#february">FEBRUARY</a></li>
           <li class="category"><a href="#february">FEBRUARY</a></li>
           <li class="category"><a href="#march">MARCH</a></li>
           <li class="category"><a href="#march">MARCH</a></li>
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           <li class="category"><a href="#september">SEPTEMBER</a></li>
           <li class="category"><a href="#september">SEPTEMBER</a></li>
           <li class="category"><a href="#october">OCTOBER</a></li>
           <li class="category"><a href="#october">OCTOBER</a></li>
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          <li class="category"><a href="#november">NOVEMBER</a></li>
 
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<div class="main">
<div class="main">
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    <div id="notebook" class="container">
 
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    <div class="header">
 
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        <h1 id="header1">Notebook</h1>
 
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Suspendisse et metus bibendum, dapibus nisl eget, facilisis dolor. Donec nec urna pellentesque, adipiscing est at, interdum risus. Integer interdum ut tellus ac imperdiet. Nam sem orci, ornare tempus leo quis, faucibus dapibus nulla. Cras rutrum lectus magna, id iaculis velit rutrum a. Etiam malesuada erat magna, in euismod nisi mattis et. Etiam malesuada pretium tempus. Fusce dictum porta dignissim.</p>
 
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</div>
 
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</div>
 
-
 
 
     <div id="january" class="container january">
     <div id="january" class="container january">
       <h1 id="header1">January</h1>
       <h1 id="header1">January</h1>
-
     <div>
+
<p id="para">-Background research<br>
 +
</p>
 +
     </div>
 +
<br><br>
     <div id="february" class="container february">
     <div id="february" class="container february">
       <h1 id="header1">February</h1>
       <h1 id="header1">February</h1>
-
     <div>
+
<p id="para">-Background research<br>
 +
</p>
 +
     </div>
 +
<br><br>
     <div id="march" class="container march">
     <div id="march" class="container march">
       <h1 id="header1">March</h1>
       <h1 id="header1">March</h1>
 +
<p id="para">
 +
-Selection of topic<br>
 +
-Further research<br>
 +
-Selection of projec<br>
 +
</p>
     </div>
     </div>
 +
<br><br>
     <div id="april" class="container april">
     <div id="april" class="container april">
       <h1 id="header1">April</h1>
       <h1 id="header1">April</h1>
 +
<p id="para">-Further research <br>
 +
</p>
     </div>
     </div>
 +
<br><br>
     <div id="may" class="container may">
     <div id="may" class="container may">
       <h1 id="header1">May</h1>
       <h1 id="header1">May</h1>
 +
<p id="para"><b><u>General Activities</b></u><br>
 +
Regular team meetings<br>
 +
Looking for sponsors<br>
 +
Month planning<br>
 +
</p>
     </div>
     </div>
 +
<br><br>
     <div id="june" class="container june">
     <div id="june" class="container june">
       <h1 id="header1">June</h1>
       <h1 id="header1">June</h1>
 +
<p id="para"><b><u>Wetlab</u></b><br>
 +
- Arrival of the biobricks<br>
 +
- The designs for the plasmids are approved and ordered Primers<br>
 +
- Preparation of culture media, agar plates, antibiotics and glycerol<br>
 +
- Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX<br>
 +
 +
</p>
     </div>
     </div>
 +
<br><br>
     <div id="july" class="container july">
     <div id="july" class="container july">
       <h1 id="header1">July</h1>
       <h1 id="header1">July</h1>
 +
<p id="para"><b><u>Wetlab</b></u><br>
 +
<b>Week 27: 30 June – 6 July</b><br>
 +
Plasmid amplification  and culturing BBa_K811005(Penn) + CPX<br>
 +
Vector and insert Digestions<br>
 +
BBa_K811005(Penn) + CPX insert ligate pET29a <br>
 +
Colony PCR <br>
 +
<br>
 +
<b>Week 28: 7 July – 13 July</b><br>
 +
Colony PCR <br>
 +
Site directed mutagenesis of CPX <br>
 +
<br>
 +
<b>Week 29: 14 July – 20 July</b><br>
 +
Site directed mutagenesis of CPX <br>
 +
Sequencing of CPX and BBa_K811005(Penn)<br>
 +
<br>
 +
<b>Week 30: 21 July – 27 July</b><br>
 +
Sequencing results came in and were as expected<br>
 +
Protein expression <br>
 +
FACS – DBCO-PEG4-5/6-TAMRA <br>
 +
<br>
 +
<b>Week 31: 28 July – August 3</b><br>
 +
Protein expression<br>
 +
FACS – DBCO-PEG10kDa <br>
 +
 +
</p>
     </div>
     </div>
 +
 +
<br><br>
     <div id="august" class="container august">
     <div id="august" class="container august">
       <h1 id="header1">August</h1>
       <h1 id="header1">August</h1>
 +
<p id="para"><b><u>Wetlab</b></u><br>
 +
<b>Week 32: 4 August – 10 August</b><br>
 +
Protein expression <br>
 +
FACS – Antibody titration <br>
 +
Protein expression curve <br>
 +
<br>
 +
<b>Week 33: 11 August – 17 August</b><br>
 +
FACS - DBCO PEG-5kDa fluorescent <br>
 +
<br>
 +
<b>Week 34: 18 August – 24 August</b><br>
 +
Start Bio-bricking<br>
 +
Start silencing mutagenesis <br>
 +
<br>
 +
<b>Week 35: 25 August – 31 August</b><br>
 +
</p>
     </div>
     </div>
      
      
 +
<br><br>
     <div id="september" class="container september">
     <div id="september" class="container september">
       <h1 id="header1">September</h1>
       <h1 id="header1">September</h1>
 +
<p id="para"><b><u>Wetlab</b></u><br>
 +
<b>Week 36: 1 September – 7 September</b><br>
 +
<br>
 +
<b>Week 37: 8 September – 14 September</b><br>
 +
<br>
 +
<b>Week 38: 15 September – 21 September</b><br>
 +
<br>
 +
<b>Week 39: 22 September – 28 September</b><br>
 +
</p>
     </div>
     </div>
 +
<br><br>
     <div id="october" class="container october">
     <div id="october" class="container october">
       <h1 id="header1">October</h1>
       <h1 id="header1">October</h1>
 +
<p id="para"><b><u>Wetlab</b></u><br>
 +
<b>Week 40: 29 September – 5 October</b><br>
 +
<br>
 +
<b>Week 41: 6 October – 12 October</b><br>
 +
<br>
 +
<b>Week 42: 13 October – 19 October</b><br>
 +
</p>
     </div>
     </div>
      
      
 +
<br><br>
-
    <div id="november" class="container november">
+
 
-
      <h1 id="header1">November</h1>
+
-
    </div>
+
-
     </div>
+
     </div></div></div>
 +
 
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Latest revision as of 12:58, 25 August 2014

TU Eindhoven iGEM Team

Notebook

January

-Background research



February

-Background research



March

-Selection of topic
-Further research
-Selection of projec



April

-Further research



May

General Activities
Regular team meetings
Looking for sponsors
Month planning



June

Wetlab
- Arrival of the biobricks
- The designs for the plasmids are approved and ordered Primers
- Preparation of culture media, agar plates, antibiotics and glycerol
- Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX



July

Wetlab
Week 27: 30 June – 6 July
Plasmid amplification and culturing BBa_K811005(Penn) + CPX
Vector and insert Digestions
BBa_K811005(Penn) + CPX insert ligate pET29a
Colony PCR

Week 28: 7 July – 13 July
Colony PCR
Site directed mutagenesis of CPX

Week 29: 14 July – 20 July
Site directed mutagenesis of CPX
Sequencing of CPX and BBa_K811005(Penn)

Week 30: 21 July – 27 July
Sequencing results came in and were as expected
Protein expression
FACS – DBCO-PEG4-5/6-TAMRA

Week 31: 28 July – August 3
Protein expression
FACS – DBCO-PEG10kDa



August

Wetlab
Week 32: 4 August – 10 August
Protein expression
FACS – Antibody titration
Protein expression curve

Week 33: 11 August – 17 August
FACS - DBCO PEG-5kDa fluorescent

Week 34: 18 August – 24 August
Start Bio-bricking
Start silencing mutagenesis

Week 35: 25 August – 31 August



September

Wetlab
Week 36: 1 September – 7 September

Week 37: 8 September – 14 September

Week 38: 15 September – 21 September

Week 39: 22 September – 28 September



October

Wetlab
Week 40: 29 September – 5 October

Week 41: 6 October – 12 October

Week 42: 13 October – 19 October



Created by iGEM Team TU Eindhoven 2014

Retrieved from "http://2014.igem.org/Team:TU_Eindhoven/Notebook"