Team:TU Eindhoven/Notebook

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<li class="category active"><a href="#january">JANUARY</a></li>
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        <h1>Notebook</h1>
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     <div id="january" class="container january">
     <div id="january" class="container january">
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       <h1>January</h1>
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       <h1 id="header1">January</h1>
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<p id="para">-Background research<br>
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<div>
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</p>
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  <h2>January 28th </h2>
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    The first meeting. <br>
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The members of the team have gotten to know each other and set up a plan to brain storm.
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We also looked into the possibilities for research in Eindhoven to determine a track for our project</p>
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<br><br>
     <div id="february" class="container february">
     <div id="february" class="container february">
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       <h1>February</h1>
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       <h1 id="header1">February</h1>
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<p id="para">-Background research<br>
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<div class = "para">
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  <h2>February 12th </h2>
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  The subjects found in January's brainstorm session were researched by subgroups of our team.
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Then we presented the ideas to each other and we voted for the most promising ideas to take a closer look.
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</p>
</p>
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</div>
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    </div>
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<div class = "para">
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<br><br>
-
  <h2>February 24th </h2>
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  We assigned roles to all the members of the team. These roles included responsibilities for Human Recources, finances, sponsoring, health and safety, modeling, lab work, and public relations.
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</br>
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The brainstorm ideas were reviewed again. And by means of a discussion we decided that our general idea would focus around the use of bacteria in the human body.
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 +
    <div id="march" class="container march">
 +
      <h1 id="header1">March</h1>
 +
<p id="para">
 +
-Selection of topic<br>
 +
-Further research<br>
 +
-Selection of projec<br>
</p>
</p>
-
</div>
 
-
 
     </div>
     </div>
-
    <div id="march" class="container march">
 
-
      <h1>March</h1>
 
-
    </div>
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<br><br>
 +
 
     <div id="april" class="container april">
     <div id="april" class="container april">
-
       <h1>April</h1>
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       <h1 id="header1">April</h1>
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+
<p id="para">-Further research <br>
 +
</p>
     </div>
     </div>
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    <div id="may" class="container may">
 
-
      <h1>May</h1>
 
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    </div>
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<br><br>
-
    <div id="june" class="container june">
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-
      <h1>June</h1>
+
 +
    <div id="may" class="container may">
 +
      <h1 id="header1">May</h1>
 +
<p id="para"><b><u>General Activities</b></u><br>
 +
Regular team meetings<br>
 +
Looking for sponsors<br>
 +
Month planning<br>
 +
</p>
     </div>
     </div>
-
    <div id="july" class="container july">
 
-
      <h1>July</h1>
 
-
   
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<br><br>
 +
    <div id="june" class="container june">
 +
      <h1 id="header1">June</h1>
 +
<p id="para"><b><u>Wetlab</u></b><br>
 +
- Arrival of the biobricks<br>
 +
- The designs for the plasmids are approved and ordered Primers<br>
 +
- Preparation of culture media, agar plates, antibiotics and glycerol<br>
 +
- Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX<br>
 +
</p>
 +
    </div>
 +
<br><br>
-
  <h2>July 7th - July 13th</h2>
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    <div id="july" class="container july">
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    Deze week hebben we ook hele leuke dingen gedaan.</p>
+
      <h1 id="header1">July</h1>
-
 
+
<p id="para"><b><u>Wetlab</b></u><br>
 +
<b>Week 27: 30 June – 6 July</b><br>
 +
Plasmid amplification  and culturing BBa_K811005(Penn) + CPX<br>
 +
Vector and insert Digestions<br>
 +
BBa_K811005(Penn) + CPX insert ligate pET29a <br>
 +
Colony PCR <br>
 +
<br>
 +
<b>Week 28: 7 July – 13 July</b><br>
 +
Colony PCR <br>
 +
Site directed mutagenesis of CPX <br>
 +
<br>
 +
<b>Week 29: 14 July – 20 July</b><br>
 +
Site directed mutagenesis of CPX <br>
 +
Sequencing of CPX and BBa_K811005(Penn)<br>
 +
<br>
 +
<b>Week 30: 21 July – 27 July</b><br>
 +
Sequencing results came in and were as expected<br>
 +
Protein expression <br>
 +
FACS – DBCO-PEG4-5/6-TAMRA <br>
 +
<br>
 +
<b>Week 31: 28 July – August 3</b><br>
 +
Protein expression<br>
 +
FACS – DBCO-PEG10kDa <br>
 +
</p>
 +
    </div>
 +
<br><br>
-
</div>
+
    <div id="august" class="container august">
-
+
      <h1 id="header1">August</h1>
 +
<p id="para"><b><u>Wetlab</b></u><br>
 +
<b>Week 32: 4 August – 10 August</b><br>
 +
Protein expression <br>
 +
FACS – Antibody titration <br>
 +
Protein expression curve <br>
 +
<br>
 +
<b>Week 33: 11 August – 17 August</b><br>
 +
FACS - DBCO PEG-5kDa fluorescent <br>
 +
<br>
 +
<b>Week 34: 18 August – 24 August</b><br>
 +
Start Bio-bricking<br>
 +
Start silencing mutagenesis <br>
 +
<br>
 +
<b>Week 35: 25 August – 31 August</b><br>
 +
</p>
 +
    </div>
 +
   
 +
<br><br>
 +
    <div id="september" class="container september">
 +
      <h1 id="header1">September</h1>
 +
<p id="para"><b><u>Wetlab</b></u><br>
 +
<b>Week 36: 1 September – 7 September</b><br>
 +
<br>
 +
<b>Week 37: 8 September – 14 September</b><br>
 +
<br>
 +
<b>Week 38: 15 September – 21 September</b><br>
 +
<br>
 +
<b>Week 39: 22 September – 28 September</b><br>
 +
</p>
 +
    </div>
 +
<br><br>
 +
    <div id="october" class="container october">
 +
      <h1 id="header1">October</h1>
 +
<p id="para"><b><u>Wetlab</b></u><br>
 +
<b>Week 40: 29 September – 5 October</b><br>
 +
<br>
 +
<b>Week 41: 6 October – 12 October</b><br>
 +
<br>
 +
<b>Week 42: 13 October – 19 October</b><br>
 +
</p>
 +
    </div>
 +
   
 +
<br><br>
 +
 
-
<div id="august" class="container august">
 
-
      <h1>August</h1>
 
-
     </div>
+
     </div></div></div>
-
    <div id="september" class="container september">
+
-
      <h1>September</h1>
+
-
    </div>
 
-
    <div id="october" class="container october">
 
-
      <h1>October</h1>
 
-
    </div>
 
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Latest revision as of 12:58, 25 August 2014

TU Eindhoven iGEM Team

Notebook

January

-Background research



February

-Background research



March

-Selection of topic
-Further research
-Selection of projec



April

-Further research



May

General Activities
Regular team meetings
Looking for sponsors
Month planning



June

Wetlab
- Arrival of the biobricks
- The designs for the plasmids are approved and ordered Primers
- Preparation of culture media, agar plates, antibiotics and glycerol
- Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX



July

Wetlab
Week 27: 30 June – 6 July
Plasmid amplification and culturing BBa_K811005(Penn) + CPX
Vector and insert Digestions
BBa_K811005(Penn) + CPX insert ligate pET29a
Colony PCR

Week 28: 7 July – 13 July
Colony PCR
Site directed mutagenesis of CPX

Week 29: 14 July – 20 July
Site directed mutagenesis of CPX
Sequencing of CPX and BBa_K811005(Penn)

Week 30: 21 July – 27 July
Sequencing results came in and were as expected
Protein expression
FACS – DBCO-PEG4-5/6-TAMRA

Week 31: 28 July – August 3
Protein expression
FACS – DBCO-PEG10kDa



August

Wetlab
Week 32: 4 August – 10 August
Protein expression
FACS – Antibody titration
Protein expression curve

Week 33: 11 August – 17 August
FACS - DBCO PEG-5kDa fluorescent

Week 34: 18 August – 24 August
Start Bio-bricking
Start silencing mutagenesis

Week 35: 25 August – 31 August



September

Wetlab
Week 36: 1 September – 7 September

Week 37: 8 September – 14 September

Week 38: 15 September – 21 September

Week 39: 22 September – 28 September



October

Wetlab
Week 40: 29 September – 5 October

Week 41: 6 October – 12 October

Week 42: 13 October – 19 October



Created by iGEM Team TU Eindhoven 2014

Retrieved from "http://2014.igem.org/Team:TU_Eindhoven/Notebook"