Team:TU Eindhoven/Notebook

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<h2>Notebook</h2>
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          <li class="category active"><a href="#notebook">NOTEBOOK</a></li>
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<li class="category active"><a href="#january">JANUARY</a></li>
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           <li class="category"><a href="#february">FEBRUARY</a></li>
           <li class="category"><a href="#february">FEBRUARY</a></li>
           <li class="category"><a href="#march">MARCH</a></li>
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  <span>Current solution</span>   
 
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      <p class="para">Bacteria can be made undetectable to the immune system with the use of encapsulation. Hydrogels are suitable for this purpose. Hydrogels are water-absorbing (synthetic) polymers, and are therefore able to form a layer around the bacteria. Due the low reactivity of this hydrogel capsule towards the immune system and permeability to small essential molecules (for example nutrients and wastes). With the use of microfluidic techniques, the amount of bacterial cells per liquid droplet is relatively easy to control. In the produced microfluidic droplet, hydrogel formation can be induced. The result is a hydrogelation from the outside towards the core of the droplet, surrounding the entire group of bacteria. The problems with these current techniques are: inhomogeneous hydrogelation (due to gelation form the outside to the core), uncontrollable cell growth inside the encapsulation, thus incontrollable drug release, and non-degradable encapsulations. The conventional encapsulation technique is visualised in Figure 1. </p>
 
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      </div><div class="header">
 
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        <h1>Notebook</h1>
 
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<div class = "infotext">
 
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<p>iGEM TU Eindhoven - Microencapsulation</p><br>
 
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<p>On this page our progress is kept neatly in chronological order</p>
 
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     <div id="january" class="container january">
     <div id="january" class="container january">
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       <h1>January</h1>
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       <h1 id="header1">January</h1>
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<p id="para">-Background research<br>
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<div class = "infotext">
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</p>
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  <h2>January 28th </h2>
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    The first meeting. <br>
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The members of the team have gotten to know each other and set up a plan to brain storm.
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We also looked into the possibilities for research in Eindhoven to determine a track for our project</p>
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<br><br>
     <div id="february" class="container february">
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       <h1>February</h1>
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       <h1 id="header1">February</h1>
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<p id="para">-Background research<br>
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<div class = "infotext">
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  <h2>February 12th </h2>
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  The subjects found in January's brainstorm session were researched by subgroups of our team.
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Then we presented the ideas to each other and we voted for the most promising ideas to take a closer look.
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</p>
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<br><br>
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  <h2>February 24th </h2>
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  We assigned roles to all the members of the team. These roles included responsibilities for Human Recources, finances, sponsoring, health and safety, modeling, lab work, and public relations.
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</br>
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The brainstorm ideas were reviewed again. And by means of a discussion we decided that our general idea would focus around the use of bacteria in the human body.
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 +
    <div id="march" class="container march">
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      <h1 id="header1">March</h1>
 +
<p id="para">
 +
-Selection of topic<br>
 +
-Further research<br>
 +
-Selection of projec<br>
</p>
</p>
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</div>
 
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     </div>
     </div>
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    <div id="march" class="container march">
 
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      <h1>March</h1>
 
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    </div>
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<br><br>
 +
 
     <div id="april" class="container april">
     <div id="april" class="container april">
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       <h1>April</h1>
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       <h1 id="header1">April</h1>
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<p id="para">-Further research <br>
 +
</p>
     </div>
     </div>
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    <div id="may" class="container may">
 
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      <h1>May</h1>
 
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    </div>
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<br><br>
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    <div id="june" class="container june">
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      <h1>June</h1>
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 +
    <div id="may" class="container may">
 +
      <h1 id="header1">May</h1>
 +
<p id="para"><b><u>General Activities</b></u><br>
 +
Regular team meetings<br>
 +
Looking for sponsors<br>
 +
Month planning<br>
 +
</p>
     </div>
     </div>
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    <div id="july" class="container july">
 
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      <h1>July</h1>
 
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<br><br>
 +
    <div id="june" class="container june">
 +
      <h1 id="header1">June</h1>
 +
<p id="para"><b><u>Wetlab</u></b><br>
 +
- Arrival of the biobricks<br>
 +
- The designs for the plasmids are approved and ordered Primers<br>
 +
- Preparation of culture media, agar plates, antibiotics and glycerol<br>
 +
- Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX<br>
 +
</p>
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    </div>
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<br><br>
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  <h2>July 7th - July 13th</h2>
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    <div id="july" class="container july">
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    Deze week hebben we ook hele leuke dingen gedaan.</p>
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      <h1 id="header1">July</h1>
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+
<p id="para"><b><u>Wetlab</b></u><br>
 +
<b>Week 27: 30 June – 6 July</b><br>
 +
Plasmid amplification  and culturing BBa_K811005(Penn) + CPX<br>
 +
Vector and insert Digestions<br>
 +
BBa_K811005(Penn) + CPX insert ligate pET29a <br>
 +
Colony PCR <br>
 +
<br>
 +
<b>Week 28: 7 July – 13 July</b><br>
 +
Colony PCR <br>
 +
Site directed mutagenesis of CPX <br>
 +
<br>
 +
<b>Week 29: 14 July – 20 July</b><br>
 +
Site directed mutagenesis of CPX <br>
 +
Sequencing of CPX and BBa_K811005(Penn)<br>
 +
<br>
 +
<b>Week 30: 21 July – 27 July</b><br>
 +
Sequencing results came in and were as expected<br>
 +
Protein expression <br>
 +
FACS – DBCO-PEG4-5/6-TAMRA <br>
 +
<br>
 +
<b>Week 31: 28 July – August 3</b><br>
 +
Protein expression<br>
 +
FACS – DBCO-PEG10kDa <br>
 +
</p>
 +
    </div>
 +
<br><br>
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</div>
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    <div id="august" class="container august">
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      <h1 id="header1">August</h1>
 +
<p id="para"><b><u>Wetlab</b></u><br>
 +
<b>Week 32: 4 August – 10 August</b><br>
 +
Protein expression <br>
 +
FACS – Antibody titration <br>
 +
Protein expression curve <br>
 +
<br>
 +
<b>Week 33: 11 August – 17 August</b><br>
 +
FACS - DBCO PEG-5kDa fluorescent <br>
 +
<br>
 +
<b>Week 34: 18 August – 24 August</b><br>
 +
Start Bio-bricking<br>
 +
Start silencing mutagenesis <br>
 +
<br>
 +
<b>Week 35: 25 August – 31 August</b><br>
 +
</p>
 +
    </div>
 +
   
 +
<br><br>
 +
    <div id="september" class="container september">
 +
      <h1 id="header1">September</h1>
 +
<p id="para"><b><u>Wetlab</b></u><br>
 +
<b>Week 36: 1 September – 7 September</b><br>
 +
<br>
 +
<b>Week 37: 8 September – 14 September</b><br>
 +
<br>
 +
<b>Week 38: 15 September – 21 September</b><br>
 +
<br>
 +
<b>Week 39: 22 September – 28 September</b><br>
 +
</p>
 +
    </div>
 +
<br><br>
 +
    <div id="october" class="container october">
 +
      <h1 id="header1">October</h1>
 +
<p id="para"><b><u>Wetlab</b></u><br>
 +
<b>Week 40: 29 September – 5 October</b><br>
 +
<br>
 +
<b>Week 41: 6 October – 12 October</b><br>
 +
<br>
 +
<b>Week 42: 13 October – 19 October</b><br>
 +
</p>
 +
    </div>
 +
   
 +
<br><br>
 +
 
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<div id="august" class="container august">
 
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      <h1>August</h1>
 
-
     </div>
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     </div></div></div>
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    <div id="september" class="container september">
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      <h1>September</h1>
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-
    </div>
 
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    <div id="october" class="container october">
 
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      <h1>October</h1>
 
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    </div>
 
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Latest revision as of 12:58, 25 August 2014

TU Eindhoven iGEM Team

Notebook

January

-Background research



February

-Background research



March

-Selection of topic
-Further research
-Selection of projec



April

-Further research



May

General Activities
Regular team meetings
Looking for sponsors
Month planning



June

Wetlab
- Arrival of the biobricks
- The designs for the plasmids are approved and ordered Primers
- Preparation of culture media, agar plates, antibiotics and glycerol
- Amplification of PET-29a(+), pEVOL-pAzF, BBa_K811005(Penn) & CPX



July

Wetlab
Week 27: 30 June – 6 July
Plasmid amplification and culturing BBa_K811005(Penn) + CPX
Vector and insert Digestions
BBa_K811005(Penn) + CPX insert ligate pET29a
Colony PCR

Week 28: 7 July – 13 July
Colony PCR
Site directed mutagenesis of CPX

Week 29: 14 July – 20 July
Site directed mutagenesis of CPX
Sequencing of CPX and BBa_K811005(Penn)

Week 30: 21 July – 27 July
Sequencing results came in and were as expected
Protein expression
FACS – DBCO-PEG4-5/6-TAMRA

Week 31: 28 July – August 3
Protein expression
FACS – DBCO-PEG10kDa



August

Wetlab
Week 32: 4 August – 10 August
Protein expression
FACS – Antibody titration
Protein expression curve

Week 33: 11 August – 17 August
FACS - DBCO PEG-5kDa fluorescent

Week 34: 18 August – 24 August
Start Bio-bricking
Start silencing mutagenesis

Week 35: 25 August – 31 August



September

Wetlab
Week 36: 1 September – 7 September

Week 37: 8 September – 14 September

Week 38: 15 September – 21 September

Week 39: 22 September – 28 September



October

Wetlab
Week 40: 29 September – 5 October

Week 41: 6 October – 12 October

Week 42: 13 October – 19 October



Created by iGEM Team TU Eindhoven 2014

Retrieved from "http://2014.igem.org/Team:TU_Eindhoven/Notebook"