Team:Bielefeld-CeBiTec/Notebook/Primer

From 2014.igem.org

(Difference between revisions)
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<td width="70%">5‘-CGCATCTTCCCTACTAGACACTCTAGAAGCGGCCGCGAATTCC-3‘</td>
<td width="70%">5‘-CGCATCTTCCCTACTAGACACTCTAGAAGCGGCCGCGAATTCC-3‘</td>
</tr>
</tr>
-
 
+
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">Second part</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">fw_pSB1C3_csoS4A (47 bp)</td>
 +
<td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGAAATCATGCAAGTTGAGAAAACG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">rv_csoS4A_PstI (43 bp)</td>
 +
<td width="70%">5‘-GAGGGATAAGACTTGCTTCCGGCAGCCTCTCGTGCTGCCGATG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">fw_PstI_csoS4A (51 bp)</td>
 +
<td width="70%">5‘-TCATCGGCAGCACGAGAGGCTGCCGGAAGCAAGTCTTATCCCTCTGATTTG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">rv_SpeI_Intergen (52 bp)</td>
 +
<td width="70%">5‘-GTCGTTGCCATAACAGATCCTCTAAAACTAGGTTGAGCACGTTCAGAACGAG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">fw_SpeI_Intergen (49 bp)</td>
 +
<td width="70%">5‘-TCGTTCTGAACGTGCTCAACCTAGTTTTAGAGGATCTGTTATGGCAACG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">rv-csoS1-pSB1C3 (50 bp)</td>
 +
<td width="70%">5‘-CGGACTGCAGCGGCCGCTACTAGTATTAGCTATTCAGATTTGCGATACAC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">fw-csoS1-pSB1C3 (46 bp)</td>
 +
<td width="70%">5‘-GTGTATCGCAAATCTGAATAGCTAATACTAGTAGCGGCCGCTGCAG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">rv-pSB1C3-csoS4A (47 bp)</td>
 +
<td width="70%">5‘-AACGTTTTCTCAACTTGCATGATTTCTCTAGAAGCGGCCGCGAATTC-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">Gluco switch</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">pSB1C3_pre_sRNApfkA (43 bp)</td>
 +
<td width="70%">5‘-TGTGGCTAAAAGAATTAGTACTCTAGAAGCGGCCGCGAATTCC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">pSB1C3_suf_sRNApfkA (43 bp)</td>
 +
<td width="70%">5‘-TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTC-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">Assays</th>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">General primer</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">int_RBS_fw (1) (42 bp)</td>
 +
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATGCATCACGGGAGATGCACTAG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">RBS_int_rev (2) (44 bp)</td>
 +
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCATTTCTCCTCTTTCCCTATAGTG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">RBS_rev (3) (22 bp)</td>
 +
<td width="70%">5‘-TTTCTCCTCTTTCCCTATAGTG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">int_fw (4) (22 bp)</td>
 +
<td width="70%">5‘-TGCATCACGGGAGATGCACTAG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">int_pSB1C3_rev (5) (50 bp)</td>
 +
<td width="70%">5‘-GACTGCAGCGGCCGCTACTAGTATCATTGAAGCTGCCACAAGGCAGGAAC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">pSB1C3_int_fw (6) (46 bp)</td>
 +
<td width="70%">5‘-CTGCCTTGTGGCAGCTTCAATGATACTAGTAGCGGCCGCTGCAGTC-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">SBPase (glpX)</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">SBPase_purify_fw (45 bp)</td>
 +
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGAGGGAATTGAAAAGCGAAAAG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">SBPase_purify_rev (53 bp)</td>
 +
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACGTAAAATACCAATTTATTGAAGCTTATCAT-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">fbaA</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">fbaA_purify_fw (50 bp)</td>
 +
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGTCTAAGATTTTTGATTTCGTAAAACC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">fbaA_purify_rev (41 bp)</td>
 +
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACAGAACGTCGATCGCGTTC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">fbaA_PstI_fw (42 bp)</td>
 +
<td width="70%">5‘-CAAAGCGAACGAAGCTTATCTGCAAGGTCAGCTGGGTAACCC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">fbaA_PstI_rev (43 bp)</td>
 +
<td width="70%">5‘-CTTTCGGGTTACCCAGCTGACCTTGCAGATAAGCTTCGTTCGC-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">tktA</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">tktA_purify_fw (43 bp)</td>
 +
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGTCCTCACGTAAAGAGCTTG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">tktA_purify_rev (43 bp)</td>
 +
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACAGCAGTTCTTTTGCTTTCGC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">tktA_PstI_fw (41 bp)</td>
 +
<td width="70%">5‘-GTTCGGTCCGCTGTTGCCGGAACTCCTCGGCGGTTCTGCTG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">tktA_PstI_rev (42 bp)</td>
 +
<td width="70%">5‘-CAGGTCAGCAGAACCGCCGAGGAGTTCCGGCAACAGCGGACC-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">cg_pgk</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">cg_pgk_purify_fw (42 bp)</td>
 +
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGGCTGTTAAGACCCTCAAG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">cg_pgk_purify_rev (42 bp)</td>
 +
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACTGAGCGAGAATTGCAACGC-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">gapA</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">gapA_purify_fw (47 bp)</td>
 +
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGACTATCAAAGTAGGTATCAACGG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">gapA_purify_rev (43 bp)</td>
 +
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCATTTGGAGATGTGAGCGATCAG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">gapA_NotI_fw (44 bp)</td>
 +
<td width="70%">5‘-CTCACAAAGACTGGCGCGGCGGCCGTGGCGCTTCCCAGAACATC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">gapA_NotI_rev (43 bp)</td>
 +
<td width="70%">5‘-GATGATGTTCTGGGAAGCGCCACGGCCGCCGCGCCAGTCTTTG-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">prkA</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">prkA_purify_fw (40 bp)</td>
 +
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGAGCAAGCCAGATCGTG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">prkA_purify_rev (42 bp)</td>
 +
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCAAACGCTAGCGGCAACCGGTG-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">pykF</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">pykF_purify_fw (47 bp)</td>
 +
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGAAAAAGACCAAAATTGTTTGCAC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">pykF_purify_rev (42 bp)</td>
 +
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACAGGACGTGAACAGATGCGG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">pykF_PstI_fw (48 bp)</td>
 +
<td width="70%">5‘-ACCGTCTGGGTAAAGAACTGGCTCTGCAAAGCGGTCTGGCACACAAAG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">pykF_PstI_rev (43 bp)</td>
 +
<td width="70%">5‘-CACCTTTGTGTGCCAGACCGCTTTGCAGAGCCAGTTCTTTACC-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">ldhA</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">ldhA_purify_fw (44 bp)</td>
 +
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGAAACTCGCCGTTTATAGCAC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">ldhA_purify_rev (42 bp)</td>
 +
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCAAACCAGTTCGTTCGGGCAGG-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">glk</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">glk_purify_fw (44 bp)</td>
 +
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGACAAAGTATGCATTAGTCGG-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">glk_purify_rev (43 bp)</td>
 +
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACAGAATGTGACCTAAGGTCTG-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">zwf</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">zwf_purify_fw (41 bp)</td>
 +
<td width="70%">5‘-CTATAGGGAAAGAGGAGAAATATGGCGGTAACGCAAACAGC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">zwf_purify_rev (45 bp)</td>
 +
<td width="70%">5‘-CTAGTGCATCTCCCGTGATGCACTCAAACTCATTCCAGGAACGAC-3‘</td>
 +
</tr>
 +
<tr bgcolor="#D8D8D8">
 +
  <th colspan="2" align="left">Gensynthesen</th>
 +
</tr>
 +
<tr>
 +
<td width="30%">pSB1C3_pre_rbcLS (44 bp)</td>
 +
<td width="70%">5‘-CATCTAGTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">pSB1C3_suf_rbcLS (43 bp)</td>
 +
<td width="70%">5‘-ATCGTCCGGGCCGCTACTAA-TACTAGTAGCGGCCGCTGCAGTC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">pSB1C3_pre_ccbLS (44 bp)</td>
 +
<td width="70%">5‘-CATCTAGTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">pSB1C3_suf_ccbLS (43 bp)</td>
 +
<td width="70%">5‘-TGGTTTACCGCGGCAACTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">pSB1C3_pre_prkA (43 bp)</td>
 +
<td width="70%">5‘-ACACGATCTGGCTTGCTCATCTCTAGAAGCGGCCGCGAATTCC-3‘</td>
 +
</tr>
 +
<tr>
 +
<td width="30%">pSB1C3_suf_prkA (43 bp)</td>
 +
<td width="70%">5‘-CGGTTGCCGCTAGCGTTTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td>
 +
</tr>
</table>
</table>

Revision as of 10:46, 24 August 2014


Primerlist

Primer ID Primer sequence
Module 1
Gene synthesis
pSB1C3_pre_Fum_A (43 bp) 5‘-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘
pSB1C3_suf_Fum_A (43 bp) 5‘-CCAAAGCGCCGAATGCGTAATACTAGTAGCGGCCGCTGCAGTC-3‘
pSB1C3_pre_Fum_BCD (42 bp) 5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘
pSB1C3_suf_Fum_BCD (43 bp) 5‘-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘
CCM-Cassette (E. coli)
ccm_fwd (58 bp) 5‘-CTCAGTCCTAGGTATTGTGCAGAGAAAGAGGAGAAAATGCTTGAAGCCAGAGAGTTAC-3‘
ccm_rev (47 bp) 5‘-TGCAGCGGCCGCTACTAGTATTATTTACTCTCCTGCGGCGACAAATG-3‘
pSB1C3_suf_ccm (50 bp) 5‘-CATTTGTCGCCGCAGGAGAGTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘
pSB1C3_pre_ccm (28 bp) 5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘
Cytochrom C (Geobacter sulfurreducens)
pccH_fwd (55 bp) 5‘-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3‘
pccH_rev (40 bp) 5‘-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3‘
pSB1C3_pre_pccH (58 bp) 5‘-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘
pSB1C3_suf_pccH (43 bp) 5‘-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3‘
??? (Julian)
T7_frd_Ec_fwd (64 bp) 5‘-TAATACGACTCACTATAGGGAAAGAGGAGAAATACTAGATGCAAACCTTTCAAGCCGATCTTGC-3‘
T7_frd_Ec_rev (44 bp) 5‘-TGCAGCGGCCGCTACTAGTATTAGATTGTAACGACACCAATCAG-3‘
pSB1C3_frd_pre (45 bp) 5‘-GCAAGATCGGCTTGAAAGGTTTGCATCTAGTATTTCTCCTCTTTC-3‘
pSB1C3_frd_suf (47 bp) 5‘-CTGATTGGTGTCGTTACAATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘
pR6K_dcuB_short (22 bp) 5‘-AATTAACCCTCACTAAAGGGCG-3‘
Module 2
Carbonic anhydrase
csoS3_can_fwd (50 bp) 5‘-ATTCGCGGCCGCTTCTAGAGTTAAGTAAAGTGTAACGATCATGAACACCC-3‘
pSB1C3_pre_can (46 bp) 5‘-GTTCATGATCGTTACACTTTACTTAACTCTAGAAGCGGCCGCGAAT-3‘
csoS3_can_rev (41 bp) 5‘-TGCAGCGGCCGCTACTAGTATCATGCGGATGCAACCTCTTC-3‘
pSB1C3_suf_can (46 bp) 5‘-TCGAAGAGGTTGCATCCGCATGATACTAGTAGCGGCCGCTGCAGTC-3‘
Shell associated protein
csoS2_sap_1_fwd (43) 5‘-ATTCGCGGCCGCTTCTAGAGGTCAAGTCTGTCATTGCGCTGTG-3‘
pSB1C3_pre_sap_1 (43 bp) 5‘-AGCGCAATGACAGACTTGACCTCTAGAAGCGGCCGCGAATTCC-3‘
csoS2_sap_1_rev (38 bp) 5‘-GCGCCGAACATGACTTTATCCGCTTGTGAAGGCTTTGC-3‘
csoS1D
csoS1D_fwd (40 bp) 5‘-ATTCGCGGCCGCTTCTAGAGTGTCTAGTAGGGAAGATGCG-3‘
csoS1D_rev (41 bp) 5‘-TGCAGCGGCCGCTACTAGTATTAGAACCCTTCAGCGCGACG-3‘
pSB1C3_suf_csoS1D (43bp) 5‘-GTCGCGCTGAAGGGTTCTAATACTAGTAGCGGCCGCTGCAGTC-3‘
pSB1C3_pre_csoS1D (43 bp) 5‘-CGCATCTTCCCTACTAGACACTCTAGAAGCGGCCGCGAATTCC-3‘
Second part
fw_pSB1C3_csoS4A (47 bp) 5‘-ATTCGCGGCCGCTTCTAGAGAAATCATGCAAGTTGAGAAAACG-3‘
rv_csoS4A_PstI (43 bp) 5‘-GAGGGATAAGACTTGCTTCCGGCAGCCTCTCGTGCTGCCGATG-3‘
fw_PstI_csoS4A (51 bp) 5‘-TCATCGGCAGCACGAGAGGCTGCCGGAAGCAAGTCTTATCCCTCTGATTTG-3‘
rv_SpeI_Intergen (52 bp) 5‘-GTCGTTGCCATAACAGATCCTCTAAAACTAGGTTGAGCACGTTCAGAACGAG-3‘
fw_SpeI_Intergen (49 bp) 5‘-TCGTTCTGAACGTGCTCAACCTAGTTTTAGAGGATCTGTTATGGCAACG-3‘
rv-csoS1-pSB1C3 (50 bp) 5‘-CGGACTGCAGCGGCCGCTACTAGTATTAGCTATTCAGATTTGCGATACAC-3‘
fw-csoS1-pSB1C3 (46 bp) 5‘-GTGTATCGCAAATCTGAATAGCTAATACTAGTAGCGGCCGCTGCAG-3‘
rv-pSB1C3-csoS4A (47 bp) 5‘-AACGTTTTCTCAACTTGCATGATTTCTCTAGAAGCGGCCGCGAATTC-3‘
Gluco switch
pSB1C3_pre_sRNApfkA (43 bp) 5‘-TGTGGCTAAAAGAATTAGTACTCTAGAAGCGGCCGCGAATTCC-3‘
pSB1C3_suf_sRNApfkA (43 bp) 5‘-TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTC-3‘
Assays
General primer
int_RBS_fw (1) (42 bp) 5‘-CTATAGGGAAAGAGGAGAAATGCATCACGGGAGATGCACTAG-3‘
RBS_int_rev (2) (44 bp) 5‘-CTAGTGCATCTCCCGTGATGCATTTCTCCTCTTTCCCTATAGTG-3‘
RBS_rev (3) (22 bp) 5‘-TTTCTCCTCTTTCCCTATAGTG-3‘
int_fw (4) (22 bp) 5‘-TGCATCACGGGAGATGCACTAG-3‘
int_pSB1C3_rev (5) (50 bp) 5‘-GACTGCAGCGGCCGCTACTAGTATCATTGAAGCTGCCACAAGGCAGGAAC-3‘
pSB1C3_int_fw (6) (46 bp) 5‘-CTGCCTTGTGGCAGCTTCAATGATACTAGTAGCGGCCGCTGCAGTC-3‘
SBPase (glpX)
SBPase_purify_fw (45 bp) 5‘-CTATAGGGAAAGAGGAGAAATATGAGGGAATTGAAAAGCGAAAAG-3‘
SBPase_purify_rev (53 bp) 5‘-CTAGTGCATCTCCCGTGATGCACGTAAAATACCAATTTATTGAAGCTTATCAT-3‘
fbaA
fbaA_purify_fw (50 bp) 5‘-CTATAGGGAAAGAGGAGAAATATGTCTAAGATTTTTGATTTCGTAAAACC-3‘
fbaA_purify_rev (41 bp) 5‘-CTAGTGCATCTCCCGTGATGCACAGAACGTCGATCGCGTTC-3‘
fbaA_PstI_fw (42 bp) 5‘-CAAAGCGAACGAAGCTTATCTGCAAGGTCAGCTGGGTAACCC-3‘
fbaA_PstI_rev (43 bp) 5‘-CTTTCGGGTTACCCAGCTGACCTTGCAGATAAGCTTCGTTCGC-3‘
tktA
tktA_purify_fw (43 bp) 5‘-CTATAGGGAAAGAGGAGAAATATGTCCTCACGTAAAGAGCTTG-3‘
tktA_purify_rev (43 bp) 5‘-CTAGTGCATCTCCCGTGATGCACAGCAGTTCTTTTGCTTTCGC-3‘
tktA_PstI_fw (41 bp) 5‘-GTTCGGTCCGCTGTTGCCGGAACTCCTCGGCGGTTCTGCTG-3‘
tktA_PstI_rev (42 bp) 5‘-CAGGTCAGCAGAACCGCCGAGGAGTTCCGGCAACAGCGGACC-3‘
cg_pgk
cg_pgk_purify_fw (42 bp) 5‘-CTATAGGGAAAGAGGAGAAATATGGCTGTTAAGACCCTCAAG-3‘
cg_pgk_purify_rev (42 bp) 5‘-CTAGTGCATCTCCCGTGATGCACTGAGCGAGAATTGCAACGC-3‘
gapA
gapA_purify_fw (47 bp) 5‘-CTATAGGGAAAGAGGAGAAATATGACTATCAAAGTAGGTATCAACGG-3‘
gapA_purify_rev (43 bp) 5‘-CTAGTGCATCTCCCGTGATGCATTTGGAGATGTGAGCGATCAG-3‘
gapA_NotI_fw (44 bp) 5‘-CTCACAAAGACTGGCGCGGCGGCCGTGGCGCTTCCCAGAACATC-3‘
gapA_NotI_rev (43 bp) 5‘-GATGATGTTCTGGGAAGCGCCACGGCCGCCGCGCCAGTCTTTG-3‘
prkA
prkA_purify_fw (40 bp) 5‘-CTATAGGGAAAGAGGAGAAATATGAGCAAGCCAGATCGTG-3‘
prkA_purify_rev (42 bp) 5‘-CTAGTGCATCTCCCGTGATGCAAACGCTAGCGGCAACCGGTG-3‘
pykF
pykF_purify_fw (47 bp) 5‘-CTATAGGGAAAGAGGAGAAATATGAAAAAGACCAAAATTGTTTGCAC-3‘
pykF_purify_rev (42 bp) 5‘-CTAGTGCATCTCCCGTGATGCACAGGACGTGAACAGATGCGG-3‘
pykF_PstI_fw (48 bp) 5‘-ACCGTCTGGGTAAAGAACTGGCTCTGCAAAGCGGTCTGGCACACAAAG-3‘
pykF_PstI_rev (43 bp) 5‘-CACCTTTGTGTGCCAGACCGCTTTGCAGAGCCAGTTCTTTACC-3‘
ldhA
ldhA_purify_fw (44 bp) 5‘-CTATAGGGAAAGAGGAGAAATATGAAACTCGCCGTTTATAGCAC-3‘
ldhA_purify_rev (42 bp) 5‘-CTAGTGCATCTCCCGTGATGCAAACCAGTTCGTTCGGGCAGG-3‘
glk
glk_purify_fw (44 bp) 5‘-CTATAGGGAAAGAGGAGAAATATGACAAAGTATGCATTAGTCGG-3‘
glk_purify_rev (43 bp) 5‘-CTAGTGCATCTCCCGTGATGCACAGAATGTGACCTAAGGTCTG-3‘
zwf
zwf_purify_fw (41 bp) 5‘-CTATAGGGAAAGAGGAGAAATATGGCGGTAACGCAAACAGC-3‘
zwf_purify_rev (45 bp) 5‘-CTAGTGCATCTCCCGTGATGCACTCAAACTCATTCCAGGAACGAC-3‘
Gensynthesen
pSB1C3_pre_rbcLS (44 bp) 5‘-CATCTAGTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘
pSB1C3_suf_rbcLS (43 bp) 5‘-ATCGTCCGGGCCGCTACTAA-TACTAGTAGCGGCCGCTGCAGTC-3‘
pSB1C3_pre_ccbLS (44 bp) 5‘-CATCTAGTATTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘
pSB1C3_suf_ccbLS (43 bp) 5‘-TGGTTTACCGCGGCAACTAATACTAGTAGCGGCCGCTGCAGTC-3‘
pSB1C3_pre_prkA (43 bp) 5‘-ACACGATCTGGCTTGCTCATCTCTAGAAGCGGCCGCGAATTCC-3‘
pSB1C3_suf_prkA (43 bp) 5‘-CGGTTGCCGCTAGCGTTTAATACTAGTAGCGGCCGCTGCAGTC-3‘

Retrieved from "http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer"