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Team:Bielefeld-CeBiTec/Notebook/Primer
From 2014.igem.org
(Difference between revisions)
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<tr bgcolor="#D8D8D8"> | <tr bgcolor="#D8D8D8"> | ||
| - | <th colspan="2" align="left">CCM-Cassette(<i>E. coli</i>)</th> | + | <th colspan="2" align="left">CCM-Cassette (<i>E. coli</i>)</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td width="30%">pSB1C3_pre_ccm (28 bp)</td> | <td width="30%">pSB1C3_pre_ccm (28 bp)</td> | ||
<td width="70%">5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘</td> | <td width="70%">5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘</td> | ||
| + | </tr> | ||
| + | <tr bgcolor="#D8D8D8"> | ||
| + | <th colspan="2" align="left">Cytochrom C (<i>Geobacter sulfurreducens</i>)</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="30%">pccH_fwd (55 bp)</td> | ||
| + | <td width="70%">5‘-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3‘</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="30%">pccH_rev (40 bp)</td> | ||
| + | <td width="70%">5‘-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3‘</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="30%">pSB1C3_pre_pccH (58 bp)</td> | ||
| + | <td width="70%">5‘-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="30%">pSB1C3_suf_pccH (43 bp)</td> | ||
| + | <td width="70%">5‘-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3‘</td> | ||
</tr> | </tr> | ||
Revision as of 17:18, 22 August 2014
Primerlist
| Primer ID | Primer sequence |
|---|---|
| Module 1 | |
| Gene synthesis | |
| pSB1C3_pre_Fum_A (43 bp) | 5‘-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_Fum_A (43 bp) | 5‘-CCAAAGCGCCGAATGCGTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_Fum_BCD (42 bp) | 5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘ |
| pSB1C3_suf_Fum_BCD (43 bp) | 5‘-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| CCM-Cassette (E. coli) | |
| ccm_fwd (58 bp) | 5‘-CTCAGTCCTAGGTATTGTGCAGAGAAAGAGGAGAAAATGCTTGAAGCCAGAGAGTTAC-3‘ |
| ccm_rev (47 bp) | 5‘-TGCAGCGGCCGCTACTAGTATTATTTACTCTCCTGCGGCGACAAATG-3‘ |
| pSB1C3_suf_ccm (50 bp) | 5‘-CATTTGTCGCCGCAGGAGAGTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘ |
| pSB1C3_pre_ccm (28 bp) | 5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘ |
| Cytochrom C (Geobacter sulfurreducens) | |
| pccH_fwd (55 bp) | 5‘-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3‘ |
| pccH_rev (40 bp) | 5‘-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3‘ |
| pSB1C3_pre_pccH (58 bp) | 5‘-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘ |
| pSB1C3_suf_pccH (43 bp) | 5‘-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3‘ |
