Team:Bielefeld-CeBiTec/Notebook/Primer

From 2014.igem.org

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<td width="30%">pSB1C3_pre_Fum_BCD (42 bp)</td>
<td width="30%">pSB1C3_pre_Fum_BCD (42 bp)</td>
<td width="70%">5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘</td>
<td width="70%">5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘</td>
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</tr>
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<tr>
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<td width="30%">pSB1C3_suf_Fum_BCD (43 bp)</td>
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<td width="70%">5‘-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘</td>
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</tr>
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<tr bgcolor="#D8D8D8">
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  <th colspan="2" align="left">CCM-Cassette(<i>E. coli</i>)</th>
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</tr>
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<tr>
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<td width="30%">ccm_fwd (58 bp)</td>
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<td width="70%">5‘-CTCAGTCCTAGGTATTGTGCAGAGAAAGAGGAGAAAATGCTTGAAGCCAGAGAGTTAC-3‘</td>
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</tr>
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<tr>
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<td width="30%">ccm_rev (47 bp)</td>
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<td width="70%">5‘-TGCAGCGGCCGCTACTAGTATTATTTACTCTCCTGCGGCGACAAATG-3‘</td>
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</tr>
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<tr>
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<td width="30%">pSB1C3_suf_ccm (50 bp)</td>
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<td width="70%">5‘-CATTTGTCGCCGCAGGAGAGTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘</td>
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</tr>
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<tr>
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<td width="30%">pSB1C3_pre_ccm (28 bp)</td>
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<td width="70%">5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘</td>
</tr>
</tr>

Revision as of 17:15, 22 August 2014


Primerlist

Primer ID Primer sequence
Module 1
Gene synthesis
pSB1C3_pre_Fum_A (43 bp) 5‘-GTCTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3‘
pSB1C3_suf_Fum_A (43 bp) 5‘-CCAAAGCGCCGAATGCGTAATACTAGTAGCGGCCGCTGCAGTC-3‘
pSB1C3_pre_Fum_BCD (42 bp) 5‘-TTGGTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATCC-3‘
pSB1C3_suf_Fum_BCD (43 bp) 5‘-GGGGTGTGGCGTCGATCTAATACTAGTAGCGGCCGCTGCAGTC-3‘
CCM-Cassette(E. coli)
ccm_fwd (58 bp) 5‘-CTCAGTCCTAGGTATTGTGCAGAGAAAGAGGAGAAAATGCTTGAAGCCAGAGAGTTAC-3‘
ccm_rev (47 bp) 5‘-TGCAGCGGCCGCTACTAGTATTATTTACTCTCCTGCGGCGACAAATG-3‘
pSB1C3_suf_ccm (50 bp) 5‘-CATTTGTCGCCGCAGGAGAGTAAATAATACTAGTAGCGGCCGCTGCAGTC-3‘
pSB1C3_pre_ccm (28 bp) 5‘-CTCTGCTAGCACAATACCTAGGACTGAG-3‘

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