Team:UT-Dallas/Notebook/8-14

From 2014.igem.org

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<UL>
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<LI> Miniprep ctxA, ctxB.
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<LI> Glycerol stock + Miniprep reporters for toxT & tcpD
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<LI> Test digest and ran gel for ctxA, ctxB.
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<LI> Gel extraction + purification of RBS-carb back bone
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<LI> Inoculate 2 colonies from each plate transformed yesterday (second batch, reporter vectors on Carb).
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<LI> Test Digest + Gel Electrophoresis Reporters on Carb
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<LI> Prepared for sequencing: diluted to the correct volume and concentration.
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<LI> Ligation + Transformation: B1 reporters vectors on Carb back bone
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<LI> Purified RBS (digested overnight yesterday).
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<LI> Inoculation more RBS for later use.
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<LI> Transformed of overnight ligation yesterday (third batch, reporter vectors with promoters under Chlora).
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<LI> Inoculate more colonies from ctxA, ctxB plates.
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<img class="pic" src="http://i.imgur.com/lm53eED.jpg" width="400px" align="right"/>
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       <br> RBS-Carb Gel was good.  
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       <br> Updating the wiki notebook from the slowest computer in the building because currently we can't log into any other computer. Rishika is gel-ing the ctx-s.
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Revision as of 20:19, 20 August 2014

Thursday, August 14, 2014

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Today's tasks:
  • Glycerol stock + Miniprep reporters for toxT & tcpD
  • Gel extraction + purification of RBS-carb back bone
  • Test Digest + Gel Electrophoresis Reporters on Carb
  • Ligation + Transformation: B1 reporters vectors on Carb back bone
  • Inoculation more RBS for later use.


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