Team:UT-Dallas/Notebook/8-10

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<LI> Miniprep potential transformants containing Reporter plasmids for ctxB, tcpJ, tcpP, and tcpS
<LI> Miniprep potential transformants containing Reporter plasmids for ctxB, tcpJ, tcpP, and tcpS
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<UL>  <LI> Clone inoculated from tcpS plate did not show expected yellow phenotype. 2 additional clones from the tcpS plate were inoculated in Chloramphenicol broth overnight to be purified and tested on Monday.  
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        <LI> Clone inoculated from tcpS plate did not show expected yellow phenotype. 2 additional clones from the tcpS plate were inoculated in Chloramphenicol broth overnight to be purified and tested on Monday.  
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Revision as of 17:57, 11 August 2014

Sunday, August 10, 2014

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Purified plasmid DNA from potential Reporter clones for later testing.

Test purified plasmid DNA for correct insert.

Today's tasks:

Miniprep

Inoculated more clones
  • Miniprep potential transformants containing Reporter plasmids for ctxB, tcpJ, tcpP, and tcpS
  • Clone inoculated from tcpS plate did not show expected yellow phenotype. 2 additional clones from the tcpS plate were inoculated in Chloramphenicol broth overnight to be purified and tested on Monday.


Preparing cells for plasmid purification.


Description of image from today

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